Abstract:
AIMS:To generate continuous minimum inhibitory concentration (MIC) data that describes the discrete nature of experimentally derived population MIC data. METHODS AND RESULTS:A logistic model was fitted to experimentally derived MIC population cumulative distributions from clinical isolates of Haemophilus influenzae, Moraxella catarrhalis, Streptococcus pneumoniae and Staphylococcus aureus (European Committee on Antimicrobial Susceptibility Testing, BSAC and MYSTIC population susceptibility databases). From the model continuous distributions of population susceptibility were generated. The experimentally observed population distributions based on discrete MIC could be reproduced from this underlying continuous distribution. Monte Carlo (MC) simulation was used to confirm findings. Where the discrete experimental data contained few or no isolates with MIC greater or less than the antimicrobial concentration range tested, the true mean MIC was a factor of 0.707 times that normally reported and may be of little clinical significance. Where data contained isolates beyond the range of concentration used, the true MIC was dependent on the SD and the number of isolates and could be clinically significant. Subpopulations of differing susceptibilities could be modelled successfully using a modified logistic equation: this allows a more accurate examination of the data from these databases. CONCLUSIONS:The mean MIC and SD of population data currently reported are incorrect as the method of obtaining such parameters relies on normally distributed data which current MIC population data are not. SIGNIFICANCE AND IMPACT OF THE STUDY:Obtaining the distribution parameters from the underlying continuous distribution of MIC can be carried out using a simple logistic equation. MC simulation using these values allows easy visualization of the discrete data. The analyses of subpopulations within the data should increase the usefulness of horizontal studies.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Lambert RJ,Lambert Rdoi
10.1111/j.1365-2672.2006.02842.xsubject
Has Abstractpub_date
2006-05-01 00:00:00pages
999-1010issue
5eissn
1364-5072issn
1365-2672pii
JAM2842journal_volume
100pub_type
杂志文章abstract:AIMS:This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. METHODS AND RESULTS:The effect of Candida infection on host cell viability was evaluated by the microscopy of t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12817
更新日期:2015-07-01 00:00:00
abstract:AIMS:To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS:Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in bi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04508.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:To evaluate the potential for using a novel chemiluminescence-based enzyme assay for rapid detection of enterococci in water contaminated with faecal waste. METHODS AND RESULTS:The novel assay (EntLight) was based on the enzymatic hydrolysis of the chemiluminescent 1,2-dioxetane [(4-methoxy-4(3-β-d-glucoside-4-ch...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05051.x
更新日期:2011-08-01 00:00:00
abstract:AIMS:To study the expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies (MAbs) C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. METHODS AND RESULTS:The reaction patterns of antibodies to L. monocytogenes held in stressful environments for a short...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02035.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02989.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:The transfer of tetO gene conferring resistance to tetracycline was studied between Campylobacter jejuni strains, in the digestive tract of chickens. METHODS AND RESULTS:In vitro conjugation experiments were first performed in order to select donor/recipient couples for further in vivo assay. Then, chickens were ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02306.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To investigate the effects of the endophyte Bacillus subtilisALB629 on the growth of cacao seedlings at early developmental stage and to evaluate its antimicrobial properties. METHODS AND RESULTS:Germinating cacao seeds were inoculated with ALB629, and seedlings growth was evaluated 30 days later. Significant inc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12485
更新日期:2014-06-01 00:00:00
abstract:AIMS:To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS:Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04210.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. METHODS AND RESULTS:One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Anti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14999
更新日期:2021-01-09 00:00:00
abstract:AIMS:To determine the occurrence of Escherichia coli harbouring virulence markers of shiga- or entero-toxins and resistance to antimicrobials in surface waters. METHODS AND RESULTS:Surface water samples were collected at six locations of the river Gomti. E. coli isolates (n = 90) were characterized for their pathogeni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03879.x
更新日期:2008-12-01 00:00:00
abstract:AIMS:To determine the 16S rRNA gene fingerprints of Bacillus thuringiensis strains to reveal phylogenetic relationships among them. METHODS AND RESULTS:Using 16S rRNA gene restriction fragment length polymorphisms generated by HindIII and EcoRI, 86 Bacillus thuringiensis strains were classified. This includes 80 B. th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01227.x
更新日期:2001-01-01 00:00:00
abstract:AIMS:To investigate the influence of micro-organisms associated with copper corrosion on 'blue water' corrosion in drinking water. METHODS AND RESULTS:Laboratory rigs comprising of polycarbonate containers attached to annealed copper plumbing tubes were filled with Melbourne drinking water and sterilized by autoclavin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02341.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Yersinia enterocolitica causes several syndromes in humans. The most common presentation is enterocolitis in children, presenting as fever and diarrhoea. A Y. enterocolitica multiple strain infection in twin infants was investigated. METHODS AND RESULTS:One isolate was recovered from one patient and two morpholog...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01252.x
更新日期:2001-03-01 00:00:00
abstract:AIMS:This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin-8 (IL-8) gene expression in the co-culture of the Bacillus and the pathogen in vitro. METHODS AND RESULTS:Bacillus subtilis, Bacillus indicus and Bacillus coagulans were initially turned to spore and h...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14898
更新日期:2020-10-15 00:00:00
abstract:AIMS:To evaluate the phenotypic expression of auto-aggregation, adhesion to mucin and biofilm formation of lactobacilli isolated from human vagina and the presence of related genes. METHODS AND RESULTS:Seven different strains of three Lactobacillus species (Lactobacillus gasseri, Lactobacillus rhamnosus and Lactobacil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12642
更新日期:2014-12-01 00:00:00
abstract:AIMS: To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. M...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05321.x
更新日期:2012-08-01 00:00:00
abstract:AIM:To isolate and identify an anticandida compound from newly isolated Fusarium strain MS-R1 and characterization of its activity against standard and clinical strains of Candida. METHODS AND RESULTS:The fungal strain, Fusarium strain MS-R1, was isolated from soil. Molecular identification according to the internal t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12493
更新日期:2014-06-01 00:00:00
abstract::The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01054.x
更新日期:2000-05-01 00:00:00
abstract:AIMS:The aims of this study were to: (1) estimate the effectiveness of ultraviolet radiation (UV) and sulfuric-acid based fertilizer (SA), at reducing levels of generic Escherichia coli in surface irrigation water and on produce and surface soil in open produce fields; and (2) describe the population dynamics of generi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.15011
更新日期:2021-01-22 00:00:00
abstract:AIMS:To determine the effects of sodium bisulfate (SBS) on the bacterial populations in cattle waste. METHODS AND RESULTS:We applied SBS at 0, 60, 70 or 100 kg week(-1) to cattle waste as it accumulated on the floors of four cattle pens, housing eight cattle each. We observed significant pH decreases in all of the tre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05047.x
更新日期:2011-08-01 00:00:00
abstract:AIMS:To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects. METHODS AND RESULTS:Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using g...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13025
更新日期:2016-03-01 00:00:00
abstract:AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04595.x
更新日期:2010-06-01 00:00:00
abstract::The effect of the kinetics of water potential variation (psi) on the viability of bacteria subjected to hyperosmotic stresses in water-glycerol solution was studied. The three bacteria used were Lactobacillus plantarum L-73, Leuconostoc mesenteroides LM057 and Escherichia coli TG1. These strains were submitted to a fi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1997.tb03303.x
更新日期:1997-01-01 00:00:00
abstract:AIM:Evaluation of the genetic variability of stolbur phytoplasma infecting grapevines, bindweeds and vegetables, collected in different central and southern Italian regions. MATERIALS AND RESULTS:Phytoplasma isolates belonging to stolbur subgroup 16SrXII-A were subjected to molecular characterization by polymerase cha...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04835.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:Few studies have compared the effectiveness of hygienic cleaning under simulated use conditions. This study compares commonly used and novel cleaning methods for food contact and hand contact surfaces in kitchens. METHODS AND RESULTS:We report results from two surveys on Norwegian consumers' cleaning procedures. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12869
更新日期:2015-08-01 00:00:00
abstract:AIMS:To compare the bacterial coliforms detected from occurrences in three zones of a water distribution system supplied by two separate water sources. METHODS AND RESULTS:Conventional and standardized protocols for identifying enterobacterial populations were applied. Additional tests to confirm isolates were include...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03141.x
更新日期:2007-03-01 00:00:00
abstract:AIMS:To develop methods to differentiate Escherichia coli O157:H7 and related serotypes by the use of amplicon length polymorphism (ALP) analysis based on identifying DNA sequence deletions within highly homologous regions of three sequenced E. coli strains. METHODS AND RESULTS:Potential primer locations along the anc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03987.x
更新日期:2009-01-01 00:00:00
abstract:AIMS:The isolation of high-quality RNA from cheese is a prerequisite for analysis of in situ gene expression of dairy micro-organisms. METHODS AND RESULTS:A method for rapid isolation of bacterial cells from cheese using cold citrate buffer followed by mechanical cell disruption was developed. RNA was extracted from e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03869.x
更新日期:2008-11-01 00:00:00
abstract:AIMS:This paper describes optimization of electrotransformation of Mu transposition complexes into Lactococcus lactis cells and identification of genes affecting nisin production. METHODS AND RESULTS:The highest transformation efficiency, 1.1 x 10(2) transformants microg(-1) of input transposon DNA, was achieved when ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03962.x
更新日期:2009-01-01 00:00:00
abstract:AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13615
更新日期:2018-01-01 00:00:00