Design and evaluation of PCR primers which differentiate Escherichia coli O157:H7 and related serotypes.

abstract：AIMS:To assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, Thermophilin 1277, production by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277, which was produced by S. thermophilus SBT1277, showed an antimicrobial activity against several lactic...

journal_title：Journal of applied microbiology

authors： Kabuki T,Uenishi H,Watanabe M,Seto Y,Nakajima H

更新日期：2007-04-01 00:00:00

abstract：AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...

journal_title：Journal of applied microbiology

authors： Pathirana E,McPherson A,Whittington R,Hick P

更新日期：2019-08-01 00:00:00

abstract：AIMS:To search for culturable Burkholderia species associated with annual ryegrass in soils from natural pastures in Portugal, with plant growth-promoting effects. METHODS AND RESULTS:Annual ryegrass seedlings were used to trap Burkholderia from two different soils in laboratory conditions. A combined approach using g...

journal_title：Journal of applied microbiology

authors： Castanheira N,Dourado AC,Kruz S,Alves PI,Delgado-Rodríguez AI,Pais I,Semedo J,Scotti-Campos P,Sánchez C,Borges N,Carvalho G,Barreto Crespo MT,Fareleira P

更新日期：2016-03-01 00:00:00

abstract：AIMS:The cell-surface display of chitinase Chi9602ΔSP was constructed on Bacillus thuringiensis BMB171 by using two repeat N-terminal regions of autolysin (Mbgn)2 as the anchoring motif. This study aimed to prepare a chitinolytic whole-cell B. thuringiensis biocatalyst with improved catalytic and antifungal activities....

journal_title：Journal of applied microbiology

authors： Tang M,Sun X,Zhang S,Wan J,Li L,Ni H

更新日期：2017-01-01 00:00:00

abstract：AIMS:To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. METHODS AND RESULTS:Processed semen was spiked with known amounts of MAP. Semen from different bulls as well as semen of diff...

journal_title：Journal of applied microbiology

authors： Herthnek D,Englund S,Willemsen PT,Bölske G

更新日期：2006-05-01 00:00:00

abstract：AIMS:To isolate and characterize bradyrhizobia that nodulate yardlong bean and sunnhemp in Guam. METHODS AND RESULTS:Bradyrhizobia populations that nodulate yardlong bean and sunnhemp in Guam were examined for genetic diversity and their relatedness to Bradyrhizobium japonicum and B. elkanii reference strains. Genomic...

journal_title：Journal of applied microbiology

authors： You Z,Marutani M,Borthakur D

更新日期：2002-01-01 00:00:00

abstract：AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was de...

journal_title：Journal of applied microbiology

authors： Passone MA,Resnik SL,Etcheverry MG

更新日期：2005-01-01 00:00:00

abstract：:The ability of artificial neural networks (ANN), fuzzy systems (FS) and multiple linear regression (MLR) to fit the biological activity surface describing the inhibition of Listeria monocytogenes by benzoic and cinnamic acid derivatives was compared. MLR and ANN were also compared for their ability to select the prope...

journal_title：Journal of applied microbiology

authors： Ramos-Nino ME,Ramirez-Rodriguez CA,Clifford MN,Adams MR

更新日期：1997-02-01 00:00:00

abstract：AIMS:We aimed to systematically understand the composting processes by a comparison of microbial communities during four full-scale composting processes. METHODS AND RESULTS:Microbial communities during the four different full-scale composting processes were analysed by denaturing gradient gel electrophoresis combined...

journal_title：Journal of applied microbiology

authors： Ishii K,Takii S

更新日期：2003-01-01 00:00:00

abstract：:An alginate gel bead artificial biofilm system was used to assay biofilm susceptibility to four biocides and to analyse the extent to which each agent penetrated the biofilm. Chlorine, glutaraldehyde, an isothiazolone, and a quaternary ammonium compound were tested on alginate-entrapped Enterobacter aerogenes in gel b...

journal_title：Journal of applied microbiology

authors： Stewart PS,Grab L,Diemer JA

更新日期：1998-09-01 00:00:00

abstract：AIMS:To establish a laboratory model to compare the effectiveness of detergent-based disinfection procedures for reducing cross-contamination risks during handling of contaminated chicken. METHODS AND RESULTS:During handling of chickens, artificially contaminated with Salmonella enteritidis PT4, the organism was widel...

journal_title：Journal of applied microbiology

authors： Barker J,Naeeni M,Bloomfield SF

更新日期：2003-01-01 00:00:00

abstract：AIM:A new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO(3) and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. METHODS AND RESULTS:Two evaluations of EC-Blue-10 were carried out. Firstly, EC-Blue-10 w...

journal_title：Journal of applied microbiology

authors： Kodaka H,Mizuochi S,Saito M,Matsuoka H

更新日期：2008-04-01 00:00:00

abstract：AIMS:Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii. METHODS AND RESULTS:Germ-free Artemia nauplii were cultured using Baker's yeast and dead Aer. hydrophila. Live Aer. hydrophila ...

journal_title：Journal of applied microbiology

authors： Gunasekara RA,Cornillie P,Casteleyn C,De Spiegelaere W,Sorgeloos P,Simoens P,Bossier P,Van den Broeck W

更新日期：2011-01-01 00:00:00

abstract：AIMS:The objective of this study was to determine antimicrobial activities of essential oils (EOs) against bovine respiratory disease (BRD) pathogens and nasopharyngeal commensal bacteria, as well as cytotoxicity in bovine turbinate (BT) cells in vitro. METHODS AND RESULTS:The chemical composition of 16 EOs was determ...

journal_title：Journal of applied microbiology

authors： Amat S,Baines D,Timsit E,Hallewell J,Alexander TW

更新日期：2019-06-01 00:00:00

abstract：AIMS:The present study was aimed at finding the optimal conditions for immobilization of Bacillus licheniformis KBR6 cells in calcium-alginate (Ca-alginate) beads and determining the operational stability during the production of tannin-acyl-hydrolase (tannase) under semicontinous cultivation. METHODS AND RESULTS:The ...

journal_title：Journal of applied microbiology

authors： Mohapatra PK,Mondal KC,Pati BR

更新日期：2007-06-01 00:00:00

abstract：AIMS:To identify proteins regulating antimicrobial peptide (AMP) resistance in Vibrio parahaemolyticus using membrane subproteome analysis. METHODS AND RESULTS:Three synthetic AMPs (Q4, Q6 and H1) and a natural one from fish (pleurocidin) were used for selection of AMP-resistant strains. Differential expression patter...

journal_title：Journal of applied microbiology

authors： Shen CJ,Kuo TY,Lin CC,Chow LP,Chen WJ

更新日期：2010-04-01 00:00:00

abstract：:Pseudomonas fluorescens DR54 showed antagonistic properties against plant pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. Antifungal activity was extractable from spent growth media, and fractionation by semi-preparative HPLC resulted in isolation of an active compound, which was identif...

journal_title：Journal of applied microbiology

authors： Nielsen TH,Christophersen C,Anthoni U,Sørensen J

更新日期：1999-07-01 00:00:00

abstract：AIMS:Algae are favourable as a biofuel source because of the potential high oil content and fast generation of biomass. However, one of the challenges for this technology is achieving high oil content while maintaining exponential or high growth of the organism. Introducing a two-stage reactor to optimize both growth a...

journal_title：Journal of applied microbiology

authors： Csavina JL,Stuart BJ,Riefler RG,Vis ML

更新日期：2011-08-01 00:00:00

abstract：AIMS:We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab sampling procedure. METHODS AND RESULTS:Bacillus anthracis Sterne or Bacillu...

journal_title：Journal of applied microbiology

authors： Hutchison JR,Piepel GF,Amidan BG,Hess BM,Sydor MA,Deatherage Kaiser BL

更新日期：2018-05-01 00:00:00

abstract：AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...

journal_title：Journal of applied microbiology

authors： Guo L,Long M,Huang Y,Wu G,Deng W,Yang X,Li B,Meng Y,Cheng L,Fan L,Zhang H,Zou L

更新日期：2015-07-01 00:00:00

abstract：AIMS:The ability of 31 Lactobacillus plantarum strains to adhere to biological matrixes was evaluated, and the molecules involved in adherence were studied. METHODS AND RESULTS:Mucin, basement membrane proteins and Caco-2 cells were used in adhesion tests. These in vitro assays, together with a yeast agglutination tes...

journal_title：Journal of applied microbiology

authors： Tallon R,Arias S,Bressollier P,Urdaci MC

更新日期：2007-02-01 00:00:00

abstract：AIMS:To investigate the effect of silver-based antimicrobial material incorporated in the inner liners of refrigerators on food safety and quality. METHODS AND RESULTS:In the first stage, the bactericidal effect was tested in the laboratory. Silver-containing samples and control plates were inoculated with different b...

journal_title：Journal of applied microbiology

authors： Kampmann Y,De Clerck E,Kohn S,Patchala DK,Langerock R,Kreyenschmidt J

更新日期：2008-06-01 00:00:00

abstract：AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences...

journal_title：Journal of applied microbiology

authors： Botti S,Bertaccini A

更新日期：2003-01-01 00:00:00

abstract：AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...

journal_title：Journal of applied microbiology

authors： Yu L,Zhang W,Liu T,Wang X,Peng J,Li S,Jin Q

更新日期：2007-12-01 00:00:00

abstract：AIMS:The aim of the present study was to describe an aerobic, mesophilic and heterotrophic bacterium, designated RA26, able to produce a medium-chain-length polyhydroxyalkanoate (PHA). It was isolated from a French Polynesian bacterial mat located in the atoll of Rangiroa. METHODS AND RESULTS:This micro-organism, on t...

journal_title：Journal of applied microbiology

authors： Simon-Colin C,Alain K,Colin S,Cozien J,Costa B,Guezennec JG,Raguénès GH

更新日期：2008-02-01 00:00:00

abstract：AIMS:To validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics Lactobacillus paracasei subsp. paracasei B112, DC205, DC215 and DC412 strains in the gut mucosa. METHODS AND RESULTS:Only the DC412 strain interacted strongly with the cells forming the ...

journal_title：Journal of applied microbiology

authors： Kourelis A,Zinonos I,Kakagianni M,Christidou A,Christoglou N,Yiannaki E,Testa T,Kotzamanidis C,Litopoulou-Tzanetaki E,Tzanetakis N,Yiangou M

更新日期：2010-01-01 00:00:00

abstract：AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...

journal_title：Journal of applied microbiology

authors： Babakhani S,Fahmi A,Katebi H,Ouria A,Majnouni-Toutakhane A,Ganbarov K,Kafil HS

更新日期：2020-10-07 00:00:00

abstract：AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...

journal_title：Journal of applied microbiology

authors： Cambon-Bonavita MA,Raguénès G,Jean J,Vincent P,Guezennec J

更新日期：2002-01-01 00:00:00

abstract：AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...

journal_title：Journal of applied microbiology

authors： Moresco V,Damazo NA,Barardi CR

更新日期：2016-02-01 00:00:00

abstract：AIMS:A number of previous studies have shown that plant extracts can inhibit formation of dental plaque. The ability of extracts of Rosmarinus officianalis L., Salvia officianalis L., unfermented cocoa, red grape seed and green tea to inhibit plaque bacteria, glucosyltransferase activity, glucan and plaque formation in...

journal_title：Journal of applied microbiology

authors： Smullen J,Finney M,Storey DM,Foster HA

更新日期：2012-10-01 00:00:00