Abstract：
:An alginate gel bead artificial biofilm system was used to assay biofilm susceptibility to four biocides and to analyse the extent to which each agent penetrated the biofilm. Chlorine, glutaraldehyde, an isothiazolone, and a quaternary ammonium compound were tested on alginate-entrapped Enterobacter aerogenes in gel beads ranging from 1.8 to 6 mm in diameter. Gel-entrapped bacteria were less susceptible to all four antimicrobial agents than were planktonic micro-organisms. The degree of kill measured in artificial biofilm gel beads depended on the size of the gel bead and the cell density at which it was loaded. Disinfection efficacy decreased as gel bead radius or cell density increased. The manifest dependence of biofilm disinfection efficacy on the physical properties of the artificial biofilm (radius and cell density) suggests the impingement of transport limitation of biocide transport into the biofilm. A previously developed theory of biocide reaction and diffusion in biofilm was tested by calculating an appropriate Thiele modulus. In accordance with the theory, the efficacy of all four biocides decreased, albeit noisily, as the Thiele modulus exceeded 1. This result demonstrates that transport limitation can impact antimicrobial performance against biofilms not only of oxidizing biocides but also of nonoxidizing agents.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Stewart PS,Grab L,Diemer JAdoi
10.1046/j.1365-2672.1998.853529.xsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
495-500issue
3eissn
1364-5072issn
1365-2672journal_volume
85pub_type
杂志文章abstract：AIM:To determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. METHODS:Putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm....
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03338.x
更新日期：2007-11-01 00:00:00
abstract：AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05263.x
更新日期：2012-05-01 00:00:00
abstract：AIMS:To investigate the involvement of oxidative stress and thiol redox state (TRS) in sclerotial differentiation of Sclerotium rolfsii and Sclerotinia sclerotiorum. METHODS AND RESULTS:Oxidative stress in these fungi was assessed by lipid peroxidation, which was higher in comparison with their nonsclerotiogenic count...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03527.x
更新日期：2008-01-01 00:00:00
abstract：AIMS:Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). METHODS AND RESULTS:Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. B...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12546
更新日期：2014-08-01 00:00:00
abstract：AIMS:The aim of this study was to determine the survival and growth characteristics of Salmonella enterica in sound and chill-injured tomatoes as influenced by co-infection with proteolytic moulds. METHODS AND RESULTS:Sound (not chill injured) raw, ripe tomatoes (Lycopersicon esculentum Mill. 'Roma') were inoculated w...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01995.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13309
更新日期：2016-12-01 00:00:00
abstract：:The purpose of this article is to review coculture fermentations in industrial biotechnology. Examples for the advantageous utilization of cocultures instead of single cultivations include the production of bulk chemicals, enzymes, food additives, antimicrobial substances and microbial fuel cells. Coculture fermentati...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2672.2009.04659.x
更新日期：2010-08-01 00:00:00
abstract：AIMS:To assess antibiotic resistance in airborne bacteria associated with coarse particulate matter (PM10) in an urban area, with specific considerations about the Staphylococcus genus. METHODS AND RESULTS:Disc diffusion test was performed on 243 microbial strains, isolated from PM10 in winter and summer and belonging...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.05018.x
更新日期：2011-06-01 00:00:00
abstract：AIMS:To assess the antimicrobial action of three natural-derived products (essential oil, decoction and hydrosol of Satureja thymbra) against biofilms, composed of useful, spoilage and pathogenic bacteria (formed as monoculture or/and mixed-culture), and to compare their efficiency with three standard acid and alkaline...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03694.x
更新日期：2008-06-01 00:00:00
abstract：:Probiotics are increasingly being added to food in order to develop products with health-promoting properties. Particularly, Saccharomyces cereviceae var. boulardii yeast is recently being investigated like a starting-culture for development of functional and probiotic foods. Although the literature is abundant on the...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.14037
更新日期：2018-10-01 00:00:00
abstract：:Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.13207
更新日期：2016-09-01 00:00:00
abstract：AIMS:Implant-associated infections arise from the formation of bacterial biofilms, which are difficult to be treated with conventional antibiotics. Therefore, there is a need for new implant functionalizations, which inhibit biofilm formation. The aim of the present study was to characterize the effect of synthetic pep...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14701
更新日期：2020-11-01 00:00:00
abstract：AIMS:The transfer of tetO gene conferring resistance to tetracycline was studied between Campylobacter jejuni strains, in the digestive tract of chickens. METHODS AND RESULTS:In vitro conjugation experiments were first performed in order to select donor/recipient couples for further in vivo assay. Then, chickens were ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02306.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:Cotton seeds are frequently treated with acid to remove fibres and reduce seed-transmitted diseases. This process also eliminates beneficial bacteria on the seed surface. The goal of this research was to seek and apply beneficial bacteria to acid delinted cotton seeds to evaluate their growth-promoting and salt st...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13414
更新日期：2017-04-01 00:00:00
abstract：AIMS:The aims of the project were threefold: to survey the use of disinfectants in the UK food industry; to assess the product and environmental microflora of selected food factories for the persistence of Listeria monocytogenes and Escherichia coli; and to determine the disinfectant resistance of any persistent strain...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：AIMS:The ComPath project is a pan-European programme dedicated to the monitoring of antimicrobial susceptibility of pathogens from diseased dogs and cats using standardized methods and centralized minimum inhibitory concentration (MIC) determination. Here, the susceptibility of major pathogens is reported from antimicr...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13287
更新日期：2016-11-01 00:00:00
abstract：AIMS:To generate stable acid-resistant Bifidobacterium strains isolated from human subjects and characterize the phenotypic changes of the acid-resistant derivatives. METHODS AND RESULTS:The ability of 20 Bifidobacterium strains isolated from human faeces to survive to simulated gastrointestinal transit was evaluated,...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03342.x
更新日期：2007-10-01 00:00:00
abstract：AIMS:To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS:Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents th...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04210.x
更新日期：2009-07-01 00:00:00
abstract：AIM:Calibration of impedance measurement was performed vs the Association Françoise de Normalisation (AFNOR) MPN method with a view to rapid enumeration of Escherichia coli in live marine bivalve molluscs. METHODS AND RESULTS:Linear regression models between log10 MPN and detection time (DT) were adjusted for several ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02218.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To study the effect of the type of antibiotic used in medicated diets against pathogens and the feeding level on the microbial biodiversity in the rabbit caecum. METHODS AND RESULTS:Three groups of eight does were given a diet unsupplemented (NAB) or with 100 ppm of bacitracin (BAC) or tiamulin (TIA). Litter size...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03309.x
更新日期：2007-10-01 00:00:00
abstract：AIMS:The application of essential oils (EOs) and their components as food preservatives is promising but requires a deeper understanding of their mechanisms of action. This study aims to evaluate the effects of thyme EO, carvacrol, citral and 2-(E)-hexenal, on whole-genome gene expression (the transcriptome), as well a...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14048
更新日期：2018-11-01 00:00:00
abstract：AIMS:To assess the efficacy of a β-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. METHODS AND RESULTS:Three different doses (0.5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,随机对照试验
doi：10.1111/jam.12713
更新日期：2015-02-01 00:00:00
abstract：AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12585
更新日期：2014-10-01 00:00:00
abstract：AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2005.02700.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:Isolates of Candida valida, Rhodotorula glutinis and Trichosporon asahii from the rhizosphere of sugar beet in Egypt were examined for their ability to colonize roots, to promote plant growth and to protect sugar beet from Rhizoctonia solani AG-2-2 diseases, under glasshouse conditions. METHODS AND RESULTS:Root c...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.02043.x
更新日期：2004-01-01 00:00:00
abstract：AIM:To develop co-aggregated bacterial inoculant comprising of Methylobacterium oryzae CBMB20/Methylobacterium suomiense CBMB120 strains with Azospirillum brasilense (CW903) strain and testing their efficiency as inoculants for plant growth promotion (PGP). METHODS AND RESULTS:Biofilm formation and co-aggregation effi...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12384
更新日期：2014-02-01 00:00:00
abstract：AIMS:To compare the bacterial coliforms detected from occurrences in three zones of a water distribution system supplied by two separate water sources. METHODS AND RESULTS:Conventional and standardized protocols for identifying enterobacterial populations were applied. Additional tests to confirm isolates were include...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03141.x
更新日期：2007-03-01 00:00:00
abstract：AIMS:To investigate the ecophysiology of populations of polyphosphate-accumulating organisms (PAO) and glycogen-accumulating organisms (GAO) in communities of a novel acetate fed process removing phosphate from wastewater. Attempts were made to see if acetate could be replaced by an alternative carbon source which did ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.03857.x
更新日期：2008-11-01 00:00:00
abstract：AIMS:Pseudomonas spp. are considered the most important milk spoilage organisms. Here we describe development of a fluorescence in situ hybridization (FISH) probe specific for detection and enumeration of Pseudomonas spp. in milk. METHODS AND RESULTS:16S rRNA sequences were analysed to develop specific oligonucleotide...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.01930.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02385.x
更新日期：2004-01-01 00:00:00
