Abstract:
AIMS:Acinetobacter baumannii is a global health problem, which threatens many healthcare settings. The current study aims to develop a detection assay for Ac. baumannii using unmodified gold nanoparticles (AuNPs). METHODS AND RESULTS:Fifty-three Ac. baumannii clinical isolates were collected from Egyptian hospitals. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction using boiling method and PCR amplification of the 23S-16S rRNA intergenic spacer sequences (ITS). AuNPs were synthesized using citrate reduction method. Detection and optimization of Ac. baumannii amplicons using unmodified spherical AuNPs were performed using species-specific DNA oligonucleotide. The nano-gold assay was able to colorimetrically detect and distinguish Ac. baumannii from other Gram-negative bacteria. The turnaround time of the assay is about 2 h including sample treatment and amplification. The assay detection limit is 0·8125 ng of DNA. CONCLUSIONS:The developed colorimetric assay is sensitive, fast and reliable and can be used for identification of Ac. baumannii. SIGNIFICANCE AND IMPACT OF THE STUDY:There is a need to develop robust, rapid, and specific methods for detection of Ac. baumannii isolated from clinical specimens. The developed nanogold assay prototype allows sensitive, specific and rapid detection of amplified DNA of A. baumannii and represents a reliable diagnostic tool to aid routine laboratory identification of this pathogen.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Khalil MA,Azzazy HM,Attia AS,Hashem AGdoi
10.1111/jam.12546subject
Has Abstractpub_date
2014-08-01 00:00:00pages
465-71issue
2eissn
1364-5072issn
1365-2672journal_volume
117pub_type
杂志文章abstract:AIMS:The aim of this study was to develop a method based on immunomagnetic capture and polymerase chain reaction (IC-PCR assay) for detection of Cryptosporidium parvum and Giardia intestinalis in sewage sludge. METHODS AND RESULTS:The detection limit of the IC-PCR assay for both organisms was 625 oocysts and cysts ml(...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01468.x
更新日期:2001-12-01 00:00:00
abstract::The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01021.x
更新日期:2000-04-01 00:00:00
abstract:AIMS:Cucumber angular leaf spot caused by Pseudomonas syringae pv. lachrymans (Psl) is an important and destructive disease worldwide, and no effective technique has been developed for the control of the pathogen. Detection of infection or latent in cucumber plants is critical to evaluate disease progress and strengthe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13356
更新日期:2017-02-01 00:00:00
abstract:AIM:Nano-biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. METHODS AND RESULTS:The pigment extracted from D. maris ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14889
更新日期:2020-10-08 00:00:00
abstract:AIMS:The purpose of this study was to investigate the inactivation kinetics of Staphylococcus aureus in a ham model system by high hydrostatic pressure at ambient (25 degrees C) and selected temperatures (45, 55 degrees C). Selective [Baird Parker (BP) agar] and nonselective [brain heart infusion (BHI) agar] growth med...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03698.x
更新日期:2008-06-01 00:00:00
abstract:AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02700.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To offset the negative effects of aeration on bacterial cellulose (BC) production by acetic acid bacteria using enmeshed cellulose microfibrils (CM) on luffa sponge matrices (LSM). METHODS AND RESULTS:The CM were enmeshed on LSM (LSM-CM). The optimal amount of LSM-CM was determined for BC production under aerated...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13290
更新日期:2016-12-01 00:00:00
abstract:AIM:To quantify microbial aerosols generated by a series of laboratory accidents and to use these data in risk assessment. METHODS AND RESULTS:A series of laboratory accident scenarios have been devised and the microbial aerosol generated by them has been measured using a range of microbial air samplers. The accident ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02798.x
更新日期:2006-04-01 00:00:00
abstract::Probiotics are increasingly being added to food in order to develop products with health-promoting properties. Particularly, Saccharomyces cereviceae var. boulardii yeast is recently being investigated like a starting-culture for development of functional and probiotic foods. Although the literature is abundant on the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14037
更新日期:2018-10-01 00:00:00
abstract:AIMS:To study the expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies (MAbs) C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. METHODS AND RESULTS:The reaction patterns of antibodies to L. monocytogenes held in stressful environments for a short...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02035.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04770.x
更新日期:2010-10-01 00:00:00
abstract::In this study, three flor strains of Saccharomyces cerevisiae were genetically characterized. They were isolated from biofilms on Sardinian sherry-like wines produced at family-run wineries where pure cultures of yeasts were not used. The study aimed to investigate the life cycle of these naturally-occurring flor stra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01163.x
更新日期:2000-10-01 00:00:00
abstract:AIMS:Apply response surface methodology (RSM) to develop and optimize an economical medium for lichenysin production, which is a surfactant produced by Bacillus licheniformis and evaluate the application of lichenysin in the prevention and disruption of pathogenic micro-organism biofilm that creates health problems in ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12992
更新日期:2016-01-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05296.x
更新日期:2012-06-01 00:00:00
abstract:AIMS:To help gain a better understanding of factors influencing the establishment within the oral cavity of Streptococcus salivarius K12, a commensal oral bacterium, we characterized its behaviour in artificial saliva. METHODS AND RESULTS:Streptococcus salivarius K12 was grown in artificial saliva complemented with a ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05077.x
更新日期:2011-09-01 00:00:00
abstract:AIMS:Investigation of waste pulps and soda pulp bleaching with xylanase (X) and laccase mediator system (LMS) alone and in conjunction (one after the other) (XLMS). METHODS AND RESULTS:Soda and different grades of waste pulp fibres [used for making three-layered duplex sheets - top layer (TL), protective layer (PL) an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03251.x
更新日期:2007-08-01 00:00:00
abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore resistance to and killing by a novel sporicide, dimethyldioxirane (DMDO) that was generated in situ from acetone and potassium peroxymonosulfate at neutral pH. METHODS AND RESULTS:Spores of B. subtilis were effectively killed by DMDO. Rates of killing by DMDO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03000.x
更新日期:2006-11-01 00:00:00
abstract:AIM:To evaluate changes in the resident microbial population in the cranial vaginal mucosa induced by a progesterone-releasing intravaginal device (PRID) compared to the vaginal microbiota of cows with reproductive disorders. METHODS AND RESULTS:Vaginal discharge was evaluated by clinical examination and a Vaginitis D...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13239
更新日期:2016-11-01 00:00:00
abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract::The genus Lactococcus comprises 12 species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium, probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13179
更新日期:2016-10-01 00:00:00
abstract:AIMS: The aim of this study was to generate temperature-sensitive Edwardsiella tarda mutant and to evaluate potential of the mutant as a vaccine in olive flounder (Paralichthys olivaceus). METHODS AND RESULTS: A temperature-sensitive E. tarda mutant was generated by replacement of alr gene promoter with cI857-λP(R) pr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05328.x
更新日期:2012-08-01 00:00:00
abstract:AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13516
更新日期:2017-09-01 00:00:00
abstract:AIMS:The environmental pathogen Streptococcus uberis causes intramammary infections in dairy cows. Because biofilm growth might contribute to Strep. uberis mastitis, we conducted a biological screen to identify genes potentially involved in the regulation of biofilm growth. METHODS AND RESULTS:By screening a transposo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12664
更新日期:2015-01-01 00:00:00
abstract:AIMS:To monitor emissions of NH(3) and N(2)O during composting and link these to ammonia oxidation rates and the community structure of ammonia oxidizing bacteria (AOB). METHODS AND RESULTS:A laboratory-scale compost reactor treating organic household waste was run for 2 months. NH(3) emissions peaked when pH started ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04111.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:Adenosine triphosphate (ATP) during the enzymatic production of glutathione is necessary. In this study, our aims were to investigate the reason for low glutathione production in Escherichia coli coupled with an ATP regeneration system and to develop a new strategy to improve the system. METHODS AND RESULTS:Gluta...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03415.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01914.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To study the genetic basis of C(4)-dicarboxylate transport (Dct) in relation to symbiotic nitrogen fixation in Mesorhizobium ciceri. METHODS AND RESULTS:A Tn5-induced mutant strain (TL16) of M. ciceri, unable to grow on C(4)-dicarboxylates, was isolated from the wild-type strain TAL 620. The mutant lacked activit...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03432.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03042.x
更新日期:2006-12-01 00:00:00
abstract:AIMS:To predict and validate survival of non-acid adapted Escherichia coli O157 in an environment mimicking the human stomach. METHODS AND RESULTS:Survival was predicted mathematically from inactivation rates at various, but constant pH values. Predictions were subsequently validated experimentally in a pH-controlled ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01865.x
更新日期:2003-01-01 00:00:00
abstract::Traditional microbiological methods are still used extensively for analysis of micro-organisms in water. However, they are inefficient due to a high labour input requirement, a low sample capacity, and often a long time lag before results are available. Analytical stages involving incubation and growth (enrichments an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.1998.tb05300.x
更新日期:1998-12-01 00:00:00