Abstract:
AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were purified from LWW02, and changes in their structure were confirmed by thin-layer chromatography and electrospray ionization mass spectrometry. Comparing with the wild-type strain BAA-894, slower growth, higher membrane permeability, higher surface hydrophobicity, stronger ability of autoaggregation and biofilm formation were observed for the mutant strain LWW02. CONCLUSIONS:The gene ESA_04107 encodes heptosyltransferase I in C. sakazakii ATCC BAA-894. The cleavage of LPS in C. sakazakii could cause its outer membrane defects and increase its ability to form biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY:The study is important for understanding the pathogenic mechanism and efficient control of C. sakazakii.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Wang L,Hu X,Tao G,Wang Xdoi
10.1111/j.1365-2672.2012.05263.xsubject
Has Abstractpub_date
2012-05-01 00:00:00pages
985-97issue
5eissn
1364-5072issn
1365-2672journal_volume
112pub_type
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