Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894.

Abstract:

AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were purified from LWW02, and changes in their structure were confirmed by thin-layer chromatography and electrospray ionization mass spectrometry. Comparing with the wild-type strain BAA-894, slower growth, higher membrane permeability, higher surface hydrophobicity, stronger ability of autoaggregation and biofilm formation were observed for the mutant strain LWW02. CONCLUSIONS:The gene ESA_04107 encodes heptosyltransferase I in C. sakazakii ATCC BAA-894. The cleavage of LPS in C. sakazakii could cause its outer membrane defects and increase its ability to form biofilms. SIGNIFICANCE AND IMPACT OF THE STUDY:The study is important for understanding the pathogenic mechanism and efficient control of C. sakazakii.

journal_name

J Appl Microbiol

authors

Wang L,Hu X,Tao G,Wang X

doi

10.1111/j.1365-2672.2012.05263.x

subject

Has Abstract

pub_date

2012-05-01 00:00:00

pages

985-97

issue

5

eissn

1364-5072

issn

1365-2672

journal_volume

112

pub_type

杂志文章