Immune gene expression in gilthead seabream (Sparus aurata) after Lymphocystis disease virus (LCDV-Sa) challenge resulting in asymptomatic infection.

Abstract:

AIMS:To determine the immune gene expression response of gilthead seabream (Sparus aurata) that is experimentally infected with the lymphocystivirus LCDV-Sa. METHODS AND RESULTS:Viral DNA and transcripts were detected by qPCR in all samples from fish injected with LCDV-Sa, demonstrating that the virus establish a systemic and asymptomatic infection. The expression of 23 immune-related genes was also analysed by RT-qPCR in the head kidney (HK) and intestine at several times post-infection (dpi). In HK, the expression of five type I interferon (IFN)-related genes (ifn, irf3, mx2, mx3 and isg15), il10 and ck10 was upregulated at 1-3 dpi, while genes related to the inflammation process (tnfα, il1ß, il6, casp1) were not differentially expressed or even downregulated. The expression profile in the intestine was different regarding type I INF-related genes. An upregulated c3 and ighm expression was observed in both HK and intestine at 3-8 dpi. Finally, the transcription of nccrp1 and mhcIIα was induced in HK, whereas tcrβ expression was downregulated in both organs. CONCLUSIONS:LCDV-Sa seems to trigger an immune response in gilthead seabream characterized by a partial activation of type I IFN system and a lack of systemic inflammatory response which may be related to viral persistence. SIGNIFICANCE AND IMPACT OF THE STUDY:The immune response observed in gilthead seabream infected by LCDV-Sa could be implicated in the establishment of an asymptomatic persistent infection.

journal_name

J Appl Microbiol

authors

Leiva-Rebollo R,Labella AM,Borrego JJ,Castro D

doi

10.1111/jam.14454

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

41-53

issue

1

eissn

1364-5072

issn

1365-2672

journal_volume

128

pub_type

杂志文章
  • Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models.

    abstract:AIMS:The pathogenic potential of Arcobacter butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays. METHODS AND RESULTS:Five of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12979

    authors: Karadas G,Bücker R,Sharbati S,Schulzke JD,Alter T,Gölz G

    更新日期:2016-01-01 00:00:00

  • Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR.

    abstract:AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatm...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04354.x

    authors: Búfalo MC,Figueiredo AS,de Sousa JP,Candeias JM,Bastos JK,Sforcin JM

    更新日期:2009-11-01 00:00:00

  • Possible mechanism of antifungal phenazine-1-carboxamide from Pseudomonas sp. against dimorphic fungi Benjaminiella poitrasii and human pathogen Candida albicans.

    abstract:AIM:Investigation of antifungal mechanism of phenazine 1-carboxamide (PC) produced by a Pseudomonas strain MCC2142. METHODS AND RESULTS:An antifungal metabolite produced by a Pseudomonas was purified and identified as PC. Human pathogenic fungi such as Candida albicans, Candida glabrata, Cryptococcus neoformans, Fusar...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12675

    authors: Tupe SG,Kulkarni RR,Shirazi F,Sant DG,Joshi SP,Deshpande MV

    更新日期:2015-01-01 00:00:00

  • A preliminary study of the effect of probiotic Streptococcus salivarius K12 on oral malodour parameters.

    abstract:AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02837.x

    authors: Burton JP,Chilcott CN,Moore CJ,Speiser G,Tagg JR

    更新日期:2006-04-01 00:00:00

  • Role of botrytized grape micro-organisms in SO2 binding phenomena.

    abstract:AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01200.x

    authors: Barbe JC,De Revel G,Joyeux A,Bertrand A,Lonvaud-Funel A

    更新日期:2001-01-01 00:00:00

  • Intrinsic tolerance of Bifidobacterium species to heat and oxygen and survival following spray drying and storage.

    abstract:AIMS:This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS:Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and most...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02648.x

    authors: Simpson PJ,Stanton C,Fitzgerald GF,Ross RP

    更新日期:2005-01-01 00:00:00

  • The role of tissue type, sampling and nucleic acid purification methodology on the inferred composition of Pacific oyster (Crassostrea gigas) microbiome.

    abstract:AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14326

    authors: Pathirana E,McPherson A,Whittington R,Hick P

    更新日期:2019-08-01 00:00:00

  • Immuno-detection of anthrose containing tetrasaccharide in the exosporium of Bacillus anthracis and Bacillus cereus strains.

    abstract:AIMS:Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium. METHODS AND RESULTS:The tetrasaccharide consists of...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04129.x

    authors: Tamborrini M,Oberli MA,Werz DB,Schürch N,Frey J,Seeberger PH,Pluschke G

    更新日期:2009-05-01 00:00:00

  • Selection of biochemical mutants of Aspergillus niger resistant to some abiotic stresses with increased inulinase production.

    abstract:AIMS:The present work aimed at evaluating the usefulness of selecting different kinds of biochemical mutants of Aspergillus niger to increase inulinase production in submerged culture. METHODS AND RESULTS:Conidia of A. niger 13/36, an active producer of inulinase, were subjected to mutagenesis with both u.v. and N-met...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.02027.x

    authors: Skowronek M,Fiedurek J

    更新日期:2003-01-01 00:00:00

  • Bactericidal mechanism of glutaraldehyde-didecyldimethylammonium bromide as a disinfectant against Escherichia coli.

    abstract:AIMS:This study focuses on the bactericidal mechanism of the new combination of disinfectant glutaraldehyde-didecyldimethylammonium bromide (GA-DDAB) against Escherichia coli. METHODS AND RESULTS:Escherichia coli were exposed to GA-DDAB, and assays for cell morphology, K+ and Ca2+ leakage, H+ -ATPase activity and DNA ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13384

    authors: Lin W,Guan X,Cao J,Niu B,Chen Q

    更新日期:2017-03-01 00:00:00

  • Characterization of Serratia marcescens surviving in disinfecting footbaths.

    abstract:AIM:To determine if disinfecting footbaths in the food industry were contaminated with bacteria and to characterize some of the bacteria present. METHODS AND RESULTS:Bacterial strains were isolated from disinfecting footbaths containing TEGO 103G (amphoteric disinfectant) or TP-99 (alkyl amino acetate-based disinfecta...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01968.x

    authors: Langsrud S,Møretrø T,Sundheim G

    更新日期:2003-01-01 00:00:00

  • Use of pulsed field gel electrophoresis as an epidemiological tool for analysis of sporadic associated strains of Salmonella typhi isolated in Taiwan.

    abstract::In order to characterize the subtypes of Salmonella typhi which cause sporadic disease in Taiwan, 55 isolates of Salm. typhi obtained from unrelated patients of sporadic cases during 1992-96 were subjected to chromosomal DNA digestion and pulsed field gel electrophoresis (PFGE). When DNAs of these 55 Salm. typhi strai...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00720.x

    authors: Tsen HY,Lin JS,Hu HH,Liu PR,Wang TK

    更新日期:1999-05-01 00:00:00

  • Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.

    abstract:AIMS:Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA)...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05189.x

    authors: Chee JY,Lau NS,Samian MR,Tsuge T,Sudesh K

    更新日期:2012-01-01 00:00:00

  • Construction of genetically engineered Streptococcus gordonii strains to provide control in QPCR assays for assessing microbiological quality of recreational water.

    abstract:AIMS:Quantitative polymerase chain reaction (QPCR) methods for beach monitoring by estimating abundance of Enterococcus spp. in recreational waters use internal, positive controls which address only the amplification of target DNA. In this study two internal, positive controls were developed to control for both amplifi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03936.x

    authors: James JB,Genthner FJ

    更新日期:2008-12-01 00:00:00

  • Diversity of antifungal and plant-associated Serratia plymuthica strains.

    abstract::A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01064.x

    authors: Berg G

    更新日期:2000-06-01 00:00:00

  • The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017.

    abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01590.x

    authors: Borah B,Thakur PS,Nigam JN

    更新日期:2002-01-01 00:00:00

  • Elevation of Francisella philomiragia subsp. noatunensis Mikalsen et al. (2007) to Francisella noatunensis comb. nov. [syn. Francisella piscicida Ottem et al. (2008) syn. nov.] and characterization of Francisella noatunensis subsp. orientalis subsp. nov.,

    abstract:AIMS:This study was conducted to clarify the taxonomic status of Francisella sp. strain Ehime-1, a fish pathogen, in relation to the fish pathogens F. piscicida and F. philomiragia subsp. noatunensis and to F. philomiragia subsp. philomiragia. METHODS AND RESULTS:Francisella sp. Ehime-1 was compared to F. piscicida, F...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04092.x

    authors: Ottem KF,Nylund A,Karlsbakk E,Friis-Møller A,Kamaishi T

    更新日期:2009-04-01 00:00:00

  • Degradation of euptox A by tannase-producing rumen bacteria from migratory goats.

    abstract:AIMS:The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng). ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13563

    authors: Sharma D,Mal G,Kannan A,Bhar R,Sharma R,Singh B

    更新日期:2017-11-01 00:00:00

  • Rapid and sensitive detection of Escherichia coli O157:H7 in bovine faeces by a multiplex PCR.

    abstract::Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00938.x

    authors: Hu Y,Zhang Q,Meitzler JC

    更新日期:1999-12-01 00:00:00

  • Ready-to-eat vegetable salads served in Nigerian restaurants: a potential source of multidrug-resistant bacteria.

    abstract:AIM:Public health promotion of fruit and vegetables as healthier sources of nutrition in Nigeria has led to an increase in consumption of fruit and vegetables, particularly in the form of ready-to-eat (RTE) salads. However, the continual association of drug-resistant bacteria with fruit and vegetables creates an additi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14693

    authors: Okafor-Elenwo EJ,Imade OS

    更新日期:2020-11-01 00:00:00

  • Bacteriophages and bacteriophage-derived endolysins as potential therapeutics to combat Gram-positive spore forming bacteria.

    abstract::Since their discovery in 1915, bacteriophages have been routinely used within Eastern Europe to treat a variety of bacterial infections. Although initially ignored by the West due to the success of antibiotics, increasing levels and diversity of antibiotic resistance is driving a renaissance for bacteriophage-derived ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.12881

    authors: Nakonieczna A,Cooper CJ,Gryko R

    更新日期:2015-09-01 00:00:00

  • Effect of NaCl-tolerant lactic acid bacteria and NaCl on the fermentation characteristics and aerobic stability of silage.

    abstract::NaCl-tolerant lactic acid bacteria (LAB) strains LC-10 (Lactobacillus casei) and LP-15 (Lact. plantarum) and NaCl were used as additives to sorghun (Sorghum bicolor). Numbers of LAB were significantly (P < 0.05) higher in all the additive-treated silages than in the control silage at an early stage of ensiling. During...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00229.x

    authors: Cai Y,Ohmomo S,Ogawa M,Kumai S

    更新日期:1997-09-01 00:00:00

  • The effects of different hygiene procedures in reducing bacterial contamination in a model domestic kitchen.

    abstract:AIMS:Few studies have compared the effectiveness of hygienic cleaning under simulated use conditions. This study compares commonly used and novel cleaning methods for food contact and hand contact surfaces in kitchens. METHODS AND RESULTS:We report results from two surveys on Norwegian consumers' cleaning procedures. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12869

    authors: Røssvoll E,Langsrud S,Bloomfield S,Moen B,Heir E,Møretrø T

    更新日期:2015-08-01 00:00:00

  • Genetic correlation between chromium resistance and reduction in Bacillus brevis isolated from tannery effluent.

    abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04326.x

    authors: Verma T,Garg SK,Ramteke PW

    更新日期:2009-11-01 00:00:00

  • Fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria.

    abstract:AIMS:This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (LAB) isolated from sugarcane grown in a Brazil on the quality of corn silage. METHODS AND RESULTS:Three strains of Lactobacillus buchneri (UFLA SLM11, UFLA SLM103 and UFLA SLM108), five strains of Lactobacillus plantarum (...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12980

    authors: Santos AO,Ávila CL,Pinto JC,Carvalho BF,Dias DR,Schwan RF

    更新日期:2016-02-01 00:00:00

  • Effect of lipopeptides of antagonistic strains of Bacillus subtilis on the morphology and ultrastructure of the cucurbit fungal pathogen Podosphaera fusca.

    abstract:AIMS:To analyse the morphological and ultrastructural effects of lipopeptides of cell-free liquid cultures from the antagonistic Bacillus subtilis strains, UMAF6614 and UMAF6639, on the cucurbit powdery mildew fungus, Podosphaera fusca, conidial germination. METHODS AND RESULTS:Butanolic extracts from cell-free supern...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03323.x

    authors: Romero D,de Vicente A,Olmos JL,Dávila JC,Pérez-García A

    更新日期:2007-10-01 00:00:00

  • Combining spore germination and heat inactivation to decontaminate materials contaminated with Bacillus anthracis spores.

    abstract:AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14474

    authors: Buhr TL,Minter ZA,Kennihan NL,Young AA,Borgers-Klonkowski EL,Osborn EB,Bohmke MD,Hamilton SM,Kimani MB,Miller CT,Mackie RS,Innocenti JM,Bensman MD,Lilly SD

    更新日期:2020-01-01 00:00:00

  • Selective inhibition of Erwinia amylovora by the herbicidally active germination-arrest factor (GAF) produced by Pseudomonas bacteria.

    abstract:AIMS: The germination-arrest factor (GAF) produced by Pseudomonas fluorescens WH6, and identified as 4-formylaminooxyvinylglycine, specifically inhibits the germination of a wide range of grassy weeds. This study was undertaken to determine whether GAF has antimicrobial activity in addition to its inhibitory effects on...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05098.x

    authors: Halgren A,Azevedo M,Mills D,Armstrong D,Thimmaiah M,McPhail K,Banowetz G

    更新日期:2011-10-01 00:00:00

  • Insights into the mechanisms of desiccation resistance of the Patagonian PAH-degrading strain Sphingobium sp. 22B.

    abstract:AIM:To analyse the physiological response of Sphingobium sp. 22B to water stress. METHODS AND RESULTS:The strain was grown under excess of carbon source and then subjected to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB) and transmission e...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13742

    authors: Madueño L,Coppotelli BM,Festa S,Alvarez HM,Morelli IS

    更新日期:2018-06-01 00:00:00

  • A simple decontamination approach using hydrogen peroxide vapour for Bacillus anthracis spore inactivation.

    abstract:AIMS:To evaluate the use of relatively low levels of hydrogen peroxide vapour (HPV) for the inactivation of Bacillus anthracis spores within an indoor environment. METHODS AND RESULTS:Laboratory-scale decontamination tests were conducted using bacterial spores of both B. anthracis Ames and Bacillus atrophaeus inoculat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13284

    authors: Wood JP,Calfee MW,Clayton M,Griffin-Gatchalian N,Touati A,Ryan S,Mickelsen L,Smith L,Rastogi V

    更新日期:2016-12-01 00:00:00