Abstract:
AIMS:This study focuses on the bactericidal mechanism of the new combination of disinfectant glutaraldehyde-didecyldimethylammonium bromide (GA-DDAB) against Escherichia coli. METHODS AND RESULTS:Escherichia coli were exposed to GA-DDAB, and assays for cell morphology, K+ and Ca2+ leakage, H+ -ATPase activity and DNA degradation were performed. GA-DDAB damaged the cell wall and disrupted cell-membrane integrity. Leakage of K+ and Ca2+ increased, resulting in significantly lower intracellular concentrations within 60 min of treatment. In addition, H+ -ATPase was inactivated and DNA was degraded. CONCLUSIONS:Leakage of intracellular components indicated that GA-DDAB damaged the cell membrane of E. coli. This may have caused the observed disruption in equilibria of metal ions, inactivation of H+ -ATPase, and DNA damage. SIGNIFICANCE AND IMPACT OF THE STUDY:Using a low concentration of GA and DDAB, a new combination disinfectant was developed. GA-DDAB displayed higher antimicrobial activity than treatment with GA or DDAB alone. Therefore, GA-DDAB may be a more cost-effective and efficient antimicrobial agent than others in use today. Furthermore, this study provided a paradigm for developing high-efficiency disinfectant to help address the growing problem of bacterial resistance.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Lin W,Guan X,Cao J,Niu B,Chen Qdoi
10.1111/jam.13384subject
Has Abstractpub_date
2017-03-01 00:00:00pages
676-685issue
3eissn
1364-5072issn
1365-2672journal_volume
122pub_type
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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