Bactericidal mechanism of glutaraldehyde-didecyldimethylammonium bromide as a disinfectant against Escherichia coli.

Abstract:

AIMS:This study focuses on the bactericidal mechanism of the new combination of disinfectant glutaraldehyde-didecyldimethylammonium bromide (GA-DDAB) against Escherichia coli. METHODS AND RESULTS:Escherichia coli were exposed to GA-DDAB, and assays for cell morphology, K+ and Ca2+ leakage, H+ -ATPase activity and DNA degradation were performed. GA-DDAB damaged the cell wall and disrupted cell-membrane integrity. Leakage of K+ and Ca2+ increased, resulting in significantly lower intracellular concentrations within 60 min of treatment. In addition, H+ -ATPase was inactivated and DNA was degraded. CONCLUSIONS:Leakage of intracellular components indicated that GA-DDAB damaged the cell membrane of E. coli. This may have caused the observed disruption in equilibria of metal ions, inactivation of H+ -ATPase, and DNA damage. SIGNIFICANCE AND IMPACT OF THE STUDY:Using a low concentration of GA and DDAB, a new combination disinfectant was developed. GA-DDAB displayed higher antimicrobial activity than treatment with GA or DDAB alone. Therefore, GA-DDAB may be a more cost-effective and efficient antimicrobial agent than others in use today. Furthermore, this study provided a paradigm for developing high-efficiency disinfectant to help address the growing problem of bacterial resistance.

journal_name

J Appl Microbiol

authors

Lin W,Guan X,Cao J,Niu B,Chen Q

doi

10.1111/jam.13384

subject

Has Abstract

pub_date

2017-03-01 00:00:00

pages

676-685

issue

3

eissn

1364-5072

issn

1365-2672

journal_volume

122

pub_type

杂志文章
  • Surface charge influences enterococcal prevalence in mixed-species biofilms.

    abstract:AIM:To study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous Enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. METHODS AND RESULTS:Six Enterococcus faecalis strains were paired with 15 other microbial isolates to form mix...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03187.x

    authors: van Merode AE,Pothoven DC,van der Mei HC,Busscher HJ,Krom BP

    更新日期:2007-05-01 00:00:00

  • Characterization and validation of a chemiluminescent assay based on a 1,2-dioxetane for rapid detection of enterococci in contaminated water and comparison with standard methods and qPCR.

    abstract:AIMS:To evaluate the potential for using a novel chemiluminescence-based enzyme assay for rapid detection of enterococci in water contaminated with faecal waste. METHODS AND RESULTS:The novel assay (EntLight) was based on the enzymatic hydrolysis of the chemiluminescent 1,2-dioxetane [(4-methoxy-4(3-β-d-glucoside-4-ch...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05051.x

    authors: Bukh AS,Roslev P

    更新日期:2011-08-01 00:00:00

  • Characterization of bacterial coliform occurrences in different zones of a drinking water distribution system.

    abstract:AIMS:To compare the bacterial coliforms detected from occurrences in three zones of a water distribution system supplied by two separate water sources. METHODS AND RESULTS:Conventional and standardized protocols for identifying enterobacterial populations were applied. Additional tests to confirm isolates were include...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03141.x

    authors: Blanch AR,Galofré B,Lucena F,Terradillos A,Vilanova X,Ribas F

    更新日期:2007-03-01 00:00:00

  • Antibiotic resistance in bacteria associated with coarse atmospheric particulate matter in an urban area.

    abstract:AIMS:To assess antibiotic resistance in airborne bacteria associated with coarse particulate matter (PM10) in an urban area, with specific considerations about the Staphylococcus genus. METHODS AND RESULTS:Disc diffusion test was performed on 243 microbial strains, isolated from PM10 in winter and summer and belonging...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05018.x

    authors: Gandolfi I,Franzetti A,Bertolini V,Gaspari E,Bestetti G

    更新日期:2011-06-01 00:00:00

  • Strain- and matrix-dependent adhesion of Lactobacillus plantarum is mediated by proteinaceous bacterial compounds.

    abstract:AIMS:The ability of 31 Lactobacillus plantarum strains to adhere to biological matrixes was evaluated, and the molecules involved in adherence were studied. METHODS AND RESULTS:Mucin, basement membrane proteins and Caco-2 cells were used in adhesion tests. These in vitro assays, together with a yeast agglutination tes...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03086.x

    authors: Tallon R,Arias S,Bressollier P,Urdaci MC

    更新日期:2007-02-01 00:00:00

  • A comparison of methods used to extract bacterial DNA from raw milk and raw milk cheese.

    abstract:AIMS:  In this study, we compare seven different methods which have been designed or modified to extract total DNA from raw milk and raw milk cheese with a view to its subsequent use for the PCR of bacterial DNA. MATERIALS AND RESULTS:  Seven extraction methods were employed to extract total DNA from these foods, and ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05294.x

    authors: Quigley L,O'Sullivan O,Beresford TP,Paul Ross R,Fitzgerald GF,Cotter PD

    更新日期:2012-07-01 00:00:00

  • Evaluation of a protocol for molecular broad-range diagnosis of culture-negative bacterial infections in clinical routine diagnosis.

    abstract:AIM:Introduction of a protocol for broad-range diagnosis of bacterial infections, which remain negative in culture. METHODS AND RESULTS:The new TaqMan real-time PCR assay amplifies part of the 16S rRNA gene. Species are identified by subsequent sequencing and phylogenetic blast analysis. The analytical sensitivity sho...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03648.x

    authors: Schabereiter-Gurtner C,Nehr M,Apfalter P,Makristathis A,Rotter ML,Hirschl AM

    更新日期:2008-04-01 00:00:00

  • Effects of sodium bisulfate on the bacterial population structure of dairy cow waste.

    abstract:AIMS:To determine the effects of sodium bisulfate (SBS) on the bacterial populations in cattle waste. METHODS AND RESULTS:We applied SBS at 0, 60, 70 or 100 kg week(-1) to cattle waste as it accumulated on the floors of four cattle pens, housing eight cattle each. We observed significant pH decreases in all of the tre...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05047.x

    authors: McGarvey JA,Stackhouse KR,Miller WG,Stanker LH,Hnasko R,Mitloehner F

    更新日期:2011-08-01 00:00:00

  • Occurrence of Helicobacter pylori in surface water in the United States.

    abstract::The primary mode of transmission of the human pathogen Helicobacter pylori is unresolved. This study examined the possibility that H. pylori is water-borne. Because methods for the direct culture of H. pylori from water samples remain elusive, a microscopic technique was used for detection of this organism. Actively r...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00912.x

    authors: Hegarty JP,Dowd MT,Baker KH

    更新日期:1999-11-01 00:00:00

  • Detection of quorum sensing signal molecules in the family Vibrionaceae.

    abstract:AIMS:The aim of this study was to detect the production of three kinds of quorum sensing (QS) signal molecules, i.e. the N-acyl-homoserine lactone (AHL), the autoinducer-2 (AI-2) and the cholerae autoinducer-1-like (CAI-1-like) molecules in 25 Vibrionaceae strains. METHODS AND RESULTS:The QS signal molecules in 25 Vib...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04998.x

    authors: Yang Q,Han Y,Zhang XH

    更新日期:2011-06-01 00:00:00

  • The composition and stability of the faecal microbiota of Merino sheep.

    abstract:AIMS:To determine the composition and temporal stability of the gut (faecal) microbiota of sheep (Ovis aries). METHODS AND RESULTS:Microbial population dynamics was conducted using ARISA (28 sheep) and 16S rRNA sequencing (11 sheep). Firmicutes and Bacteroidetes were the predominant bacterial phyla, constituting ~80% ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14468

    authors: Mamun MAA,Sandeman M,Rayment P,Brook-Carter P,Scholes E,Kasinadhuni N,Piedrafita D,Greenhill AR

    更新日期:2020-01-01 00:00:00

  • Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types.

    abstract:AIMS:To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS:Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amende...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03532.x

    authors: Fremaux B,Prigent-Combaret C,Delignette-Muller ML,Mallen B,Dothal M,Gleizal A,Vernozy-Rozand C

    更新日期:2008-01-01 00:00:00

  • Bacterial succession during curing process of a skate (Dipturus batis) and isolation of novel strains.

    abstract:AIMS:To study the succession of cultivated and uncultivated microbes during the traditional curing process of skate. METHODS AND RESULTS:The microbial diversity was evaluated by sequencing 16Sr RNA clone libraries and cultivation in variety of media from skate samples taken periodically during a 9-day curing process. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05349.x

    authors: Reynisson E,Thornór Marteinsson V,Jónsdóttir R,Magnússon SH,Hreggvidsson GO

    更新日期:2012-08-01 00:00:00

  • A novel polymer produced by a bacterium isolated from a deep-sea hydrothermal vent polychaete annelid.

    abstract:AIMS:The objective of the present work was to describe an aerobic, mesophilic and heterotrophic marine bacterium, designated HYD657, able to produce an exopolysaccharide (EPS). It was isolated from a East Pacific Rise deep-sea hydrothermal vent polychaete annelid. METHODS AND RESULTS:This micro-organism, on the basis ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01689.x

    authors: Cambon-Bonavita MA,Raguénès G,Jean J,Vincent P,Guezennec J

    更新日期:2002-01-01 00:00:00

  • Intrinsic tolerance of Bifidobacterium species to heat and oxygen and survival following spray drying and storage.

    abstract:AIMS:This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS:Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and most...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02648.x

    authors: Simpson PJ,Stanton C,Fitzgerald GF,Ross RP

    更新日期:2005-01-01 00:00:00

  • Identification of pathogenic yeast species by polymerase chain reaction amplification of the RPS0 gene intron fragment.

    abstract:AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04595.x

    authors: Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

    更新日期:2010-06-01 00:00:00

  • Metabolic defence against oxidative stress: the road less travelled so far.

    abstract::Bacteria have survived, and many have thrived, since antiquity in the presence of the highly-reactive chalcogen-oxygen (O2 ). They are known to evoke intricate strategies to defend themselves from the reactive by-products of oxygen-reactive oxygen species (ROS). Many of these detoxifying mechanisms have been extensive...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13509

    authors: Lemire J,Alhasawi A,Appanna VP,Tharmalingam S,Appanna VD

    更新日期:2017-10-01 00:00:00

  • Brettanomyces bruxellensis evolution and volatile phenols production in red wines during storage in bottles.

    abstract:AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04561.x

    authors: Coulon J,Perello MC,Lonvaud-Funel A,de Revel G,Renouf V

    更新日期:2010-04-01 00:00:00

  • Evaluation of Legionella pneumophila contamination in Italian hotel water systems by quantitative real-time PCR and culture methods.

    abstract:AIMS:This study was designed to define the extent of water contamination by Legionella pneumophila of certain Italian hotels and to compare quantitative real-time PCR with the conventional culture method. METHODS AND RESULTS:Nineteen Italian hotels of different sizes were investigated. In each hotel three hot water sa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04553.x

    authors: Bonetta S,Bonetta S,Ferretti E,Balocco F,Carraro E

    更新日期:2010-05-01 00:00:00

  • PCR-fingerprinting and RAPD approaches for tracing the source of yeast contamination in a carbonated orange juice production chain.

    abstract:AIMS:To investigate the sort and the origin of the contamination of a packed fruit juice. METHODS AND RESULTS:Fifty-eight yeast isolates were collected in a survey of two different visits to a carbonated orange juice factory. In each visit, samples were collected, six times, from seven points in the production chain. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02542.x

    authors: Pina C,Teixeiró P,Leite P,Villa M,Belloch C,Brito L

    更新日期:2005-01-01 00:00:00

  • Crenarchaeota and Euryarchaeota in temperate estuarine sediments.

    abstract:AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01297.x

    authors: Abreu C,Jurgens G,De Marco P,Saano A,Bordalo AA

    更新日期:2001-05-01 00:00:00

  • Production of massoia lactone by Aureobasidium pullulans YTP6-14 isolated from the Gulf of Thailand and its fragrant biosurfactant properties.

    abstract:AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13598

    authors: Luepongpattana S,Thaniyavarn J,Morikawa M

    更新日期:2017-12-01 00:00:00

  • Preliminary analysis of Chloroflexi populations in full-scale UASB methanogenic reactors.

    abstract:AIMS:The phylum Chloroflexi is frequently found in high abundance in methanogenic reactors, but their role is still unclear as most of them remain uncultured and understudied. Hence, a detailed analysis was performed in samples from five up-flow anaerobic sludge blanket (UASB) full-scale reactors fed different industri...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14115

    authors: Bovio P,Cabezas A,Etchebehere C

    更新日期:2019-02-01 00:00:00

  • Development of multiplex real-time PCR for simultaneous detection of three Potyviruses in tobacco plants.

    abstract:AIMS:To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus (TEV), Potato virus Y (PVY) and Tobacco vein banding mosaic virus (TVBMV). METHODS AND RESULTS:Specific primer and probe combinations for TEV and TVBMV were developed from the coa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12071

    authors: Dai J,Peng H,Chen W,Cheng J,Wu Y

    更新日期:2013-02-01 00:00:00

  • Trends, application and future prospectives of microbial carbonic anhydrase mediated carbonation process for CCUS.

    abstract::Growing industrialization and the desire for a better economy in countries has accelerated the emission of greenhouse gases (GHGs), by more than the buffering capacity of the earth's atmosphere. Among the various GHGs, carbon dioxide occupies the first position in the anthroposphere and has detrimental effects on the ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13589

    authors: Bhagat C,Dudhagara P,Tank S

    更新日期:2018-02-01 00:00:00

  • Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.

    abstract:AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04393.x

    authors: Baudoin E,Couillerot O,Spaepen S,Moënne-Loccoz Y,Nazaret S

    更新日期:2010-01-01 00:00:00

  • The regulatory effects of whey retentate from bifidobacteria fermented milk on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME).

    abstract:AIMS:To investigate the effects of whey retentate from Bifidobacteria fermented milk. METHODS AND RESULTS:The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01482.x

    authors: Meddah AT,Yazourh A,Desmet I,Risbourg B,Verstraete W,Romond MB

    更新日期:2001-12-01 00:00:00

  • Evaluation of real-time PCR methods for quantification of Acanthamoeba in anthropogenic water and biofilms.

    abstract:AIMS:To assess two real-time PCR methods (the Riviere and Qvarnstrom assays) for environmental Acanthamoeba. METHODS AND RESULTS:DNA extracted from Acanthamoeba castellanii taken from water and biofilms of cooling towers was analysed by the Riviere and Qvarnstrom assays. To quantify environmental Acanthamoeba, the cal...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04708.x

    authors: Chang CW,Wu YC,Ming KW

    更新日期:2010-09-01 00:00:00

  • RT-TGGE as a guide for the successful isolation of phosphonoacetate degrading bacteria.

    abstract:AIMS:Use of molecular techniques for the isolation of bacteria capable of phosphonoacetate mineralization as carbon, phosphorus and energy source. METHODS AND RESULTS:RNA extracts obtained at three different stages of an enrichment selecting for phosphonoacetate degrading bacteria were reverse transcribed using 16S rR...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03213.x

    authors: Panas P,McMullan G,Dooley JS

    更新日期:2007-07-01 00:00:00

  • Fermentative profile and bacterial diversity of corn silages inoculated with new tropical lactic acid bacteria.

    abstract:AIMS:This study aimed to evaluate the effects of inoculation of strains of lactic acid bacteria (LAB) isolated from sugarcane grown in a Brazil on the quality of corn silage. METHODS AND RESULTS:Three strains of Lactobacillus buchneri (UFLA SLM11, UFLA SLM103 and UFLA SLM108), five strains of Lactobacillus plantarum (...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12980

    authors: Santos AO,Ávila CL,Pinto JC,Carvalho BF,Dias DR,Schwan RF

    更新日期:2016-02-01 00:00:00