Evaluation of a protocol for molecular broad-range diagnosis of culture-negative bacterial infections in clinical routine diagnosis.

abstract：:Grab samples of drinking water collected from reservoirs and from creeks flowing over pristine land, farmland or land having mixed use were analysed for their physicochemical and microbiological characteristics. A significant difference between sites for conductivity and sites for pH was noted using a two-way ANOVA. N...

journal_title：Journal of applied microbiology

authors： Thurman R,Faulkner B,Veal D,Cramer G,Meiklejohn M

更新日期：1998-04-01 00:00:00

abstract：AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

journal_title：Journal of applied microbiology

authors： Wang L,Li Y,Mustapha A

更新日期：2009-11-01 00:00:00

abstract：AIMS:Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. METHODS AND RESULTS:One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Anti...

journal_title：Journal of applied microbiology

authors： Rodriguez Jimenez A,Dechamps E,Giaux A,Goetghebuer L,Bauwens M,Willenz P,Flahaut S,Laport MS,George IF

更新日期：2021-01-09 00:00:00

abstract：AIMS:(i) To analyse the increase in calcium ion uptake caused by several cationic dyes on Candida albicans, (ii) to postulate a mechanism, (iii) to define the effects of Zn ions on the phenomenon, and (iv) to propose the use of the dyes or their derivatives against C. albicans. METHODS AND RESULTS:Cells were grown in ...

journal_title：Journal of applied microbiology

authors： Calahorra M,Sánchez NS,Peña A

更新日期：2018-08-28 00:00:00

abstract：:A number of bacterial strains used for meat fermentations were screened for proteolytic activity. A strain of Micrococcus which was found to be proteolytic was evaluated for the effects of environmental conditions on its proteolytic activity against pork myofibrillar proteins using response surface methodology. Three ...

journal_title：Journal of applied microbiology

authors： Kenneally PM,Fransen NG,Grau H,O'Neill EE,Arendt EK

更新日期：1999-12-01 00:00:00

abstract：AIMS:Monitoring for Salmonella in slaughter pigs is important to enable targeted control measures to be applied on problem farms and at the abattoir. The aim of this study was to determine whether pooled serum and meat juice could be used to identify finishing pig herds with a high prevalence of infection. METHODS AND...

journal_title：Journal of applied microbiology

authors： Davies RH,Heath PJ,Coxon SM,Sayers AR

更新日期：2003-01-01 00:00:00

abstract：AIM:To apply a quantitative reverse-transcription PCR (qRT-PCR) method to determine the total viable count (TVC) on meat samples. METHODS AND RESULTS:Using two sets of primers to target the ribonuclease-P (RNase P) RNA transcripts of Gram-positive and Gram-negative bacteria, standard curves were generated using the Li...

journal_title：Journal of applied microbiology

authors： Dolan A,Burgess CM,Fanning S,Duffy G

更新日期：2010-07-01 00:00:00

abstract：:The effects of nisin and monolaurin, alone and in combination, were investigated on Bacillus licheniformis spores in milk at 37 degrees C. In the absence of inhibitors, germinated spores developed into growing vegetative cells and started sporulation at the end of the exponential phase. In the presence of nisin (25 IU...

journal_title：Journal of applied microbiology

authors： Mansour M,Amri D,Bouttefroy A,Linder M,Milliere JB

更新日期：1999-02-01 00:00:00

abstract：AIMS:The essential oil of Melaleuca alternifolia (tea tree) has been evaluated as a potential eco-friendly antifungal agent against Botrytis cinerea. In this study, we investigated the antifungal activity and mode of action of tea tree oil (TTO) and its components against B. cinerea. METHODS AND RESULTS:Of the compone...

journal_title：Journal of applied microbiology

authors： Yu D,Wang J,Shao X,Xu F,Wang H

更新日期：2015-11-01 00:00:00

abstract：AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...

journal_title：Journal of applied microbiology

authors： Vreulink JM,Stone W,Botha A

更新日期：2010-10-01 00:00:00

abstract：AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...

journal_title：Journal of applied microbiology

authors： Guo XL,Leng P,Yang Y,Yu LG,Lou HX

更新日期：2008-03-01 00:00:00

abstract：AIMS:In China, apple production areas are largely from the coastal to inland areas and across varied climate zones. However, the relationship among soil micro-organisms, environmental factors and fruit quality has not been clearly confirmed in orchards. Here we attempted to identify the variation of soil bacteria in th...

journal_title：Journal of applied microbiology

authors： Chai X,Yang Y,Wang X,Hao P,Wang L,Wu T,Zhang X,Xu X,Han Z,Wang Y

更新日期：2020-10-04 00:00:00

abstract：AIMS:To clarify the effects of Lactobacillus gasseri CP2305 (CP2305) on quality of life and clinical symptoms and its functional mechanisms in patients with irritable bowel syndrome (IBS). METHODS AND RESULTS:After the patients were administered CP2305 daily for 4 weeks, the IBS-severity index score was significantly ...

journal_title：Journal of applied microbiology

authors： Nobutani K,Sawada D,Fujiwara S,Kuwano Y,Nishida K,Nakayama J,Kutsumi H,Azuma T,Rokutan K

更新日期：2017-01-01 00:00:00

abstract：AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...

journal_title：Journal of applied microbiology

authors： Purchase D,Scholes LN,Revitt DM,Shutes RB

更新日期：2009-04-01 00:00:00

abstract：AIMS:To generate stable acid-resistant Bifidobacterium strains isolated from human subjects and characterize the phenotypic changes of the acid-resistant derivatives. METHODS AND RESULTS:The ability of 20 Bifidobacterium strains isolated from human faeces to survive to simulated gastrointestinal transit was evaluated,...

journal_title：Journal of applied microbiology

authors： Collado MC,Sanz Y

更新日期：2007-10-01 00:00:00

abstract：:Although there is great scientific and technological interest in examining the physiology and bioenergetics of microorganisms from extreme environments, difficulties encountered in their cultivation and lack of genetic systems hampers the investigation of these issues. As such, we have adapted methods for continuous c...

journal_title：Journal of applied microbiology

authors： Rinker KD,Han CJ,Kelly RM

更新日期：1998-12-01 00:00:00

abstract：AIMS:To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis. METHODS AND RESULTS:Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and tra...

journal_title：Journal of applied microbiology

authors： Vulevic J,Gibson GR

更新日期：2008-11-01 00:00:00

abstract：AIMS:Molecular subtyping is commonly used in foodborne disease surveillance and microbial source tracking. There is a knowledge gap regarding the molecular ecology of foodborne pathogens in non-food-associated environments. The objective of this study was to isolate and subtype foodborne pathogens from pristine natural...

journal_title：Journal of applied microbiology

authors： Ahlstrom CA,Manuel CS,Den Bakker HC,Wiedmann M,Nightingale KK

更新日期：2018-02-01 00:00:00

abstract：AIMS:The main objective of the study is to develop and improve quick bacterial tests to select the best candidates for the bioaugmentation of metal-contaminated soil, coupled with phytoextraction. METHODS AND RESULTS:Bacteria isolates (181) were selected from a collection originated from a Cu-contaminated sediment, on...

journal_title：Journal of applied microbiology

authors： Braud AM,Hubert M,Gaudin P,Lebeau T

更新日期：2015-08-01 00:00:00

abstract：AIMS:To isolate an iron-reducing bacterium and examine its ability of Fe(III) oxide reduction and dechlorination. METHODS AND RESULTS:A fermentative facultative anaerobe, strain L17 isolated from subterranean sediment, can reduce Fe(III) oxides and carbon tetrachloride (CT). It was identified as Klebsiella pneumoniae ...

journal_title：Journal of applied microbiology

authors： Li XM,Zhou SG,Li FB,Wu CY,Zhuang L,Xu W,Liu L

更新日期：2009-01-01 00:00:00

abstract：AIM:To screen a lactic acid bacteria (LAB) strain for good adaption in alfalfa ensiling and to evaluate its effects on alfalfa ensiling. METHODS AND RESULTS:Lactobacillus plantarum (LP) strain ZZU203 was selected due to its superior ability to acidify structural carbohydrate metabolite and alfalfa powder. Alfalfa was ...

journal_title：Journal of applied microbiology

authors： Zhao SS,Wang YP,Yang FY,Wang Y,Zhang H

更新日期：2020-08-01 00:00:00

abstract：AIM:This study aimed to evaluate the patterns and potential risk factors associated with the occurrence of Salmonella sp. and Escherichia coli in selected United States swine feed mills. METHODS AND RESULTS:A total of 405 samples were collected during fall 2018, spring and summer 2019 from selected sites including flo...

journal_title：Journal of applied microbiology

authors： Magossi G,Lambertini E,Noll L,Bai J,Jones C,Nagaraja TG,Phebus R,Woodworth J,Trinetta V

更新日期：2020-12-01 00:00:00

abstract：:The bactericidal activity of three bacteriocin-producing lactic acid bacteria alone and in combination with milk lactoperoxidase (LP) system activation against Listeria monocytogenes in refrigerated raw milk was studied. After 4 d at 4 degrees C, the population of L. monocytogenes in milk inoculated with bacteriocin-p...

journal_title：Journal of applied microbiology

authors： Rodríguez E,Tomillo J,Nuñez M,Medina M

更新日期：1997-09-01 00:00:00

abstract：AIMS:Filoviruses are associated with high morbidity and lethality rates in humans, are capable of human-to-human transmission, via infected material such as blood, and are believed to have low infectious doses for humans. Filoviruses are able to infect via the respiratory route and are lethal at very low doses in exper...

journal_title：Journal of applied microbiology

authors： Piercy TJ,Smither SJ,Steward JA,Eastaugh L,Lever MS

更新日期：2010-11-01 00:00:00

abstract：AIMS:To evaluate the prevalence of Cryptosporidium and Giardia in surface water supplies from the province of Alava, northern Spain, and to investigate possible associations among the presence of these pathogenic protozoa with microbiological, physicochemical and atmospheric parameters. METHODS AND RESULTS:A total of ...

journal_title：Journal of applied microbiology

authors： Carmena D,Aguinagalde X,Zigorraga C,Fernández-Crespo JC,Ocio JA

更新日期：2007-03-01 00:00:00

abstract：AIM:Nano-biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. METHODS AND RESULTS:The pigment extracted from D. maris ...

journal_title：Journal of applied microbiology

authors： Venil CK,Malathi M,Velmurugan P,Renuka Devi P

更新日期：2020-10-08 00:00:00

abstract：:The effect of yoghurt culture Rx on the survival of Staphylococcus aureus CCM 5984 added to milk in various concentrations was observed during the fermentation and storage of yoghurt. The end of the fermentation process (3.5 h) was only accompanied by a slight reduction. During the storage of yoghurt at 4 degrees C a ...

journal_title：Journal of applied microbiology

authors： Pazakova J,Turek P,Laciakova A

更新日期：1997-05-01 00:00:00

abstract：:An extracellular alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus woesei has been cloned and sequenced. The 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species P. furiosus. By using a shuttle cloning vector for halophilic bacteria,...

journal_title：Journal of applied microbiology

authors： Frillingos S,Linden A,Niehaus F,Vargas C,Nieto JJ,Ventosa A,Antranikian G,Drainas C

更新日期：2000-03-01 00:00:00

abstract：AIMS:The aim of this study was to detect the production of three kinds of quorum sensing (QS) signal molecules, i.e. the N-acyl-homoserine lactone (AHL), the autoinducer-2 (AI-2) and the cholerae autoinducer-1-like (CAI-1-like) molecules in 25 Vibrionaceae strains. METHODS AND RESULTS:The QS signal molecules in 25 Vib...

journal_title：Journal of applied microbiology

authors： Yang Q,Han Y,Zhang XH

更新日期：2011-06-01 00:00:00

abstract：AIMS:The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91. METHODS AND RESULTS:The published primers complementary to JUMPstart and gnd gene, the conserved fla...

journal_title：Journal of applied microbiology

authors： Perelle S,Dilasser F,Grout J,Fach P

更新日期：2002-01-01 00:00:00