Abstract:
AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains and was evaluated using the fractional inhibited concentration index. The effect of PLE on FLC intracellular uptake was investigated in FLC-resistant C. albicans cells by liquid chromatography-tandem mass spectrometry, and the effect on efflux drug pump was assessed by measuring the efflux of Rhodamine 123 (Rh123). PLE significantly inhibited the efflux, but not the absorption, of Rh123 in FLC-resistant strains in phosphate-buffered saline with 5% glucose. Overexpression of the multidrug-resistance gene CDR1 in FLC-resistant C. albicans isolates was detected, and the introduction of PLE to the cells showed a significant reduction of the CDR1 expression in those FLC-resistant isolates. CONCLUSIONS:These findings indicate that PLE could reverse the fungal resistant to FLC by inhibiting the efflux of FLC from C. albicans, and this effect may be related to the efflux pump. SIGNIFICANCE AND IMPACT OF THE STUDY:These results indicate that the combination of PLE and FLC may provide an approach for the clinical therapy of fungus infection induced by FLC-resistant strains.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Guo XL,Leng P,Yang Y,Yu LG,Lou HXdoi
10.1111/j.1365-2672.2007.03617.xsubject
Has Abstractpub_date
2008-03-01 00:00:00pages
831-8issue
3eissn
1364-5072issn
1365-2672pii
JAM3617journal_volume
104pub_type
杂志文章abstract:AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01310.x
更新日期:2001-05-01 00:00:00
abstract:AIMS:Salmonella Hadar, Salmonella Brancaster and Salmonella Enteritidis are the main Salmonella enterica ssp. enterica serovars isolated from poultry in Senegal. Our objective was to analyse the pulsed-field gel electrophoresis (PFGE) and antibioresistance patterns of strains belonging to these serovars and to assess t...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2005.02618.x
更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.03193.x
更新日期:2007-03-01 00:00:00
abstract:AIM:To evaluate the effect of NaCl, growth medium and temperature on the antimicrobial activity of bovine lactoferrin (LF) against Escherichia coli O157:H7 in the presence of different chelating agents. METHODS AND RESULTS:LF (32 mg ml(-1)) was tested against E. coli O157:H7 strain 3081 in Luria broth (LB) and All Pur...
journal_title:Journal of applied microbiology
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更新日期:2006-02-01 00:00:00
abstract:AIMS:As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. METHODS AND RESULTS:We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell...
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更新日期:2020-05-01 00:00:00
abstract::The genus Lactococcus comprises 12 species, some known for decades and others more recently described. Lactococcus piscium, isolated in 1990 from rainbow trout, is a psychrotrophic lactic acid bacterium, probably disregarded because most of the strains are unable to grow at 30°C. During the last 10 years, this species...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-09-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.02992.x
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2003.02079.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm m...
journal_title:Journal of applied microbiology
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更新日期:2012-12-01 00:00:00
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更新日期:2014-05-01 00:00:00
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更新日期:2018-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13465
更新日期:2017-06-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2014-11-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2021-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05047.x
更新日期:2011-08-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2010-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04411.x
更新日期:2010-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14177
更新日期:2019-03-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02428.x
更新日期:2005-01-01 00:00:00
abstract::The inactivation of Bacillus subtilis spores by ultrasonic treatments under static pressure (Mano-Sonication, MS) and a combined MS/heat treatment (Mano-Thermo-Sonication) was investigated. The sporicidal effect of MS treatments depended on static pressure, amplitude of ultrasonic waves and treatment temperature. At 7...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00593.x
更新日期:1998-11-01 00:00:00
abstract:AIMS:We have been for a species of thermophilic bacteria that can effectively decompose collagen and collagen peptides that tend to be hard-to-degrade proteins because of their high content of proline residues. This study focused upon the enzymatic degradation of prolyl peptides by thermophilic bacteria. METHODS AND R...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02210.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Probiotics have the ability to enhance the immune system, produce anti-inflammatory action and promote wound healing process. The first aim of the study was to isolate pathogenic micro-organisms from sites of chronic ulcerative lesion. The second aim was to evaluate probiotic efficacy of SYNBIO® (1:1 combination o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00