Abstract：
AIM:In the study, we investigated the regulatory effects of these genes (codY, comA, degU and spo0A) on the biosynthesis of three lipopeptides (bacillomycin D, fengycin and surfactin) in Bacillus amyloliquefaciens. METHODS AND RESULTS:The codY, comA, degU and spo0A genes in B. amyloliquefaciens fmbJ were knocked out. The results showed that the productions of bacillomycin D were significantly reduced compared with that of fmbJ. Their deletion induced great changes in the levels of transcripts specifying metabolic pathways, quorum sensing system and substance transport system in fmbJ. Moreover, overexpression of these genes improved the productions of bacillomycin D. In particular, the overexpression of spo0A enhanced bacillomycin D yield up to 648·9 ± 60·9 mg l-1 from 277·3 ± 30·5 mg l-1 . In addition, the yields of surfactin in fmbJΔcodY and fmbJΔdegU were significantly improved, and the regulatory factor CodY had no significant effect on the synthesis of fengycin. CONCLUSIONS:These genes positively regulated the expression of bacillomycin D and fengycin synthase genes in strain fmbJ. However, codY and degU negatively regulated surfactin biosynthesis. Moreover, it was found that CodY had a concentration dependence on bacillomycin D synthesis. Spo0A might play a direct regulatory role in the synthesis and secretion of bacillomycin D. SIGNIFICANCE AND IMPACT OF THE STUDY:This study indicated that genetic engineering of regulatory genes was an effective strategy to improve the yields of antimicrobial lipopeptides and provided promising strains for industrial production of lipopeptides.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Sun J,Liu Y,Lin F,Lu Z,Lu Ydoi
10.1111/jam.15007subject
Has Abstractpub_date
2021-01-18 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract：AIM:The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. METHODS AND RE...
journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
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doi：10.1046/j.1365-2672.1998.853464.x
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journal_title：Journal of applied microbiology
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doi：10.1111/j.1365-2672.2007.03661.x
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journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01651.x
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journal_title：Journal of applied microbiology
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
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journal_title：Journal of applied microbiology
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更新日期：2021-01-07 00:00:00
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journal_title：Journal of applied microbiology
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更新日期：2017-11-01 00:00:00
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journal_title：Journal of applied microbiology
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abstract：AIMS:To investigate the production of the tumour promoter 1,2-sn-diacylglycerol (DAG) by a human gut isolate of Bifidobacterium longum biovar infantis. METHODS AND RESULTS:Bifidobacterium longum biovar infantis was grown in vitro using anaerobic static batch cultures in the presence of phosphatidylcholine (PC) and tra...
journal_title：Journal of applied microbiology
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更新日期：2008-11-01 00:00:00
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
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journal_title：Journal of applied microbiology
pub_type： 杂志文章
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更新日期：2008-12-01 00:00:00
abstract：:Basil essential oils, including basil sweet linalool (BSL) and basil methyl chavicol (BMC), were screened for antimicrobial activity against a range of Gram-positive and Gram-negative bacteria, yeasts and moulds using an agar well diffusion method. Both essential oils showed antimicrobial activity against most of the ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00338.x
更新日期：1998-02-01 00:00:00
abstract：AIMS:This work was carried out to complete the sequence of the arc cluster involved in arginine catabolism in Oenococcus oeni, and particularly to characterize the genes encoding proteins involved in arginine transport. METHODS AND RESULTS:Using molecular cloning, two loci encoding proteins involved in the arginine-or...
journal_title：Journal of applied microbiology
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更新日期：2003-01-01 00:00:00
