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Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test.

Abstract：

AIMS:The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91. METHODS AND RESULTS:The published primers complementary to JUMPstart and gnd gene, the conserved flanking sequences of O-antigen genes clusters in E. coli and related species were used to amplify the 10-kbp O91 O-antigen biosynthesis locus of STEC O91. A DNA library representative of this cluster allowed two O91 specific probes to be identified, and two specific PCR O91 serotyping tests to be successfully developed. CONCLUSIONS:These results confirm that the O-antigen gene cluster sequences of E. coli allow rapidly a specific O-antigen PCR assay to be designed. SIGNIFICANCE AND IMPACT OF THE STUDY:These findings increase the number of PCR-assays available to replace the classical O-serotyping among E. coli O-antigen.

journal_name

J Appl Microbiol

journal_title

Journal of applied microbiology

authors

Perelle S,Dilasser F,Grout J,Fach P

doi

10.1046/j.1365-2672.2002.01743.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

758-64

issue

5

eissn

1364-5072

issn

1365-2672

pii

1743

journal_volume

93

pub_type

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