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Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease.

Abstract：

AIMS:The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. METHODS AND RESULTS:Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must be added in the case of freeze-drying conidia. Addition of other protectants (Tween-20, peptone, sucrose, glucose, starch and peptone + starch) to skimmed milk before freeze-drying did not improve the conidial viability which was obtained with skimmed milk alone. Glycerol had a negative effect on the lyophilization of E. nigrum conidia. Epicoccum nigrum conidia freeze-dried with skimmed milk, or fluidized bed-dried alone maintained an initial viability for 30 and 90 days, respectively, for storage at room temperature. Epicoccum nigrum conidial viability after spray-drying was lower than 10%. CONCLUSIONS:The best method to dry E. nigrum conidia was fluidized bed-drying. Conidia without protectants dried by this method had 100% viability and survived for 90 days at room temperature. SIGNIFICANCE AND IMPACT OF STUDY:This paper deals with methods for the potential formulation of a biocontrol agent which is being tested for eventual commercialization.

journal_name

J Appl Microbiol

journal_title

Journal of applied microbiology

authors

Larena I,De Cal A,Liñán M,Melgarejo P

doi

10.1046/j.1365-2672.2003.01860.x

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

508-14

issue

3

eissn

1364-5072

issn

1365-2672

pii

1860

journal_volume

94

pub_type

杂志文章

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