Protein-protein interaction network and potential drug target candidates of Streptococcus suis.

Abstract:

AIMS:This study aimed to explore potential drug targets of Streptococcus suis at the system level. METHODS AND RESULTS:A homologous protein mapping method was used in the construction of a protein-protein interaction (PPI) network of S. suis, which presented 1147 nonredundant interaction pairs among 286 proteins. The parameters of PPI networks were calculated and showed scale-free network properties. Forty-one possibly essential proteins identified from 47 highly connected proteins were selected as potential drug target candidates. Of these proteins, 30 were already regarded as drug targets in other bacterial species. Six transporters with high connections to other functional proteins were identified as probably not essential but important functional proteins. Afterward, the subnetwork centered with cell division protein FtsZ was used in confirming the PPI network through bacterial two-hybrid analysis. CONCLUSIONS:The predicted PPI network covers 13.04% of the proteome in S. suis. The selected 41 potential drug target candidates are conserved between S. suis and several model bacteria. SIGNIFICANCE AND IMPACT OF STUDY:The predictions included proteins known to be drug targets, and a verifying experiment confirmed the reliability of predicted interactions. This work is the first to present systematic computational PPI data for S. suis and provides potential drug targets, which are valuable in exploring novel anti-streptococcus drugs.

journal_name

J Appl Microbiol

authors

Tan MF,Zou G,Wei Y,Liu WQ,Li HQ,Hu Q,Zhang LS,Zhou R

doi

10.1111/jam.14950

subject

Has Abstract

pub_date

2020-11-29 00:00:00

eissn

1364-5072

issn

1365-2672

pub_type

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