Abstract:
AIMS:This study aimed to explore potential drug targets of Streptococcus suis at the system level. METHODS AND RESULTS:A homologous protein mapping method was used in the construction of a protein-protein interaction (PPI) network of S. suis, which presented 1147 nonredundant interaction pairs among 286 proteins. The parameters of PPI networks were calculated and showed scale-free network properties. Forty-one possibly essential proteins identified from 47 highly connected proteins were selected as potential drug target candidates. Of these proteins, 30 were already regarded as drug targets in other bacterial species. Six transporters with high connections to other functional proteins were identified as probably not essential but important functional proteins. Afterward, the subnetwork centered with cell division protein FtsZ was used in confirming the PPI network through bacterial two-hybrid analysis. CONCLUSIONS:The predicted PPI network covers 13.04% of the proteome in S. suis. The selected 41 potential drug target candidates are conserved between S. suis and several model bacteria. SIGNIFICANCE AND IMPACT OF STUDY:The predictions included proteins known to be drug targets, and a verifying experiment confirmed the reliability of predicted interactions. This work is the first to present systematic computational PPI data for S. suis and provides potential drug targets, which are valuable in exploring novel anti-streptococcus drugs.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Tan MF,Zou G,Wei Y,Liu WQ,Li HQ,Hu Q,Zhang LS,Zhou Rdoi
10.1111/jam.14950subject
Has Abstractpub_date
2020-11-29 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract:AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03813.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2004.02329.x
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03086.x
更新日期:2007-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00632.x
更新日期:1999-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01944.x
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abstract:AIM:To determine the contribution of enterococci and coliforms from bovine faeces and teats to contamination of raw milk. METHODS:Putative enterococci (n = 301) and coliforms (n = 365) were isolated from bovine faeces (n = 20), cows' teats (n = 20), the raw milk (n = 1) and the milking environment (n = 4) on one farm....
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03338.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2020-02-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2006-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
abstract::Nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (CLA) from free linoleic acid in vitro. Two strains of Propionibacterium freudenreichii ssp. freudenreichii and one strain ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01995.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02242.x
更新日期:2004-01-01 00:00:00
abstract::With the emergence of antibiotic resistance, the interest for antimicrobial agents has recently increased again in public health. Copper was recognized in 2008 by the United States Environmental Protection Agency (EPA) as the first metallic antimicrobial agent. This led to many investigations of the various properties...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13681
更新日期:2018-05-01 00:00:00
abstract:AIMS:To assess the biodiversity of a large number of microbial fuel cell (MFC) anodes from a variety of MFC designs, all enriched with domestic wastewater, using a molecular fingerprinting method. METHODS AND RESULTS:We optimized a protocol allowing the rapid characterization of MFC communities using terminal restrict...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04735.x
更新日期:2010-09-01 00:00:00