Abstract:
AIMS:The goal of this research is to model the effects of two osmotic solutions factors on the reduction of selected microorganisms, and to assess the application in osmotic dehydration process of animal raw materials from the aspect of microbiological safety. METHODS AND RESULTS:Sugar beet molasses and aquatic osmotic solution were prepared and inoculated with Escherichia coli, Listeria monocytogenes and Salmonella spp. Varied factors of osmotic solutions were: time, temperature and concentration of osmotic solutions. Samples of osmotic solutions were subjected to standard and modified microbiological ISO methods. The result showed that increase in factors had a positive effect on the reduction of microbiological load, while the time of the process was the most influential technological parameter. Number of L. monocytogenes was reduced to <10 CFU per g at the end of the process in the highest concentration of sugar beet molasses at all process temperatures. CONCLUSIONS:Developed mathematical models of reduction ratios for tested microorganisms were statistically significant, allowing good prediction of reduction ratio values based on applied factors. SIGNIFICANCE AND IMPACT OF THE STUDY:Obtained levels of reduction of all tested microorganisms present good base for the production of safe osmotically dehydrated products of animal origin.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Filipović I,Markov S,Filipović V,Filipović J,Vidaković A,Novković N,Rafajlovska Vdoi
10.1111/jam.13927subject
Has Abstractpub_date
2018-09-01 00:00:00pages
843-852issue
3eissn
1364-5072issn
1365-2672journal_volume
125pub_type
杂志文章abstract:AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13615
更新日期:2018-01-01 00:00:00
abstract:AIMS:To evaluate mannan oligosaccharide (MOS) and threonine effects on performance, small intestine morphology and Salmonella spp. counts in Salmonella Enteritidis-challenged birds. METHODS AND RESULTS:One-day-old chicks (1d) were distributed into five treatments: nonchallenged animals fed basal diet (RB-0), animals f...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12108
更新日期:2013-04-01 00:00:00
abstract:AIMS:To determine if nisin F-loaded self-setting brushite cement could control the growth of Staphylococcus aureus in vivo. METHODS AND RESULTS: Brushite cement was prepared by mixing equimolar concentrations of β-tricalcium phosphate and monocalcium phosphate monohydrate. Nisin F was added at 5·0, 2·5 and 1·0% (w/w)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05241.x
更新日期:2012-04-01 00:00:00
abstract:AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04358.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03042.x
更新日期:2006-12-01 00:00:00
abstract:AIMS:To predict and validate survival of non-acid adapted Escherichia coli O157 in an environment mimicking the human stomach. METHODS AND RESULTS:Survival was predicted mathematically from inactivation rates at various, but constant pH values. Predictions were subsequently validated experimentally in a pH-controlled ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01865.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02282.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12960
更新日期:2015-12-01 00:00:00
abstract::The ability of artificial neural networks (ANN), fuzzy systems (FS) and multiple linear regression (MLR) to fit the biological activity surface describing the inhibition of Listeria monocytogenes by benzoic and cinnamic acid derivatives was compared. MLR and ANN were also compared for their ability to select the prope...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:AIMS:Investigation of waste pulps and soda pulp bleaching with xylanase (X) and laccase mediator system (LMS) alone and in conjunction (one after the other) (XLMS). METHODS AND RESULTS:Soda and different grades of waste pulp fibres [used for making three-layered duplex sheets - top layer (TL), protective layer (PL) an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03251.x
更新日期:2007-08-01 00:00:00
abstract:AIMS:To evaluate a standard aerosolization method for uniformly depositing threat-representative spores onto surfaces. METHODS AND RESULTS:Lyophilized Bacillus anthracis ΔSterne spores, coated in silica, were aerosolized into a containment chamber and deposited onto nine surface types by two independent laboratories. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12509
更新日期:2014-07-01 00:00:00
abstract:AIM:To quantify microbial aerosols generated by a series of laboratory accidents and to use these data in risk assessment. METHODS AND RESULTS:A series of laboratory accident scenarios have been devised and the microbial aerosol generated by them has been measured using a range of microbial air samplers. The accident ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02798.x
更新日期:2006-04-01 00:00:00
abstract::An extracellular phenolic acid esterase produced by the fungus Penicillium expansum in solid state culture released ferulic and rho-coumaric acid from methyl esters of the acids, and from the phenolic-carbohydrate esters O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->3)-O-beta- D-xylopyranosyl-(1-->4)-D-xylopy...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00307.x
更新日期:1997-12-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a laboratory biofilm growth reactor system that simulated the toilet bowl environment and which could be used for biocide efficacy testing. METHODS AND RESULTS:A microbial biofilm reactor system incorporating intermittent flow and nutrient provision was designed. The react...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01342.x
更新日期:2001-07-01 00:00:00
abstract:AIMS:Most studies of biofilm effects on dental materials use single-species biofilms, or consortia. Microcosm biofilms grown directly from saliva or plaque are much more diverse, but difficult to characterize. We used the Human Oral Microbial Identification Microarray (HOMIM) to validate a reproducible oral microcosm m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05439.x
更新日期:2012-12-01 00:00:00
abstract:AIMS:Nutritional requirements of Lactobacillus fermentum Ogi E1 were studied in order to define a simplified fermentation medium. METHODS AND RESULTS:When grown with MRS-medium in 2l bioreactors, a biphasic pattern of growth and metabolite production was observed. Study of nutritional requirements resulted in a simpli...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01272.x
更新日期:2001-04-01 00:00:00
abstract:AIMS:To evaluate the efficacy of acidic electrolysed water (EW) in the presence of organic matter (bovine serum) on the inoculated surfaces of lettuce and spinach. MATERIALS AND RESULTS:Lettuce and spinach leaves were inoculated with a cocktail of three strains each of Escherichia coli O157:H7, Salmonella Typhimurium ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03928.x
更新日期:2008-12-01 00:00:00
abstract::Antifungal metabolites produced by Bacillus pumilus in Potato Dextrose Broth (PDB) were isolated from culture supernatant fluid by precipitation with ammonium sulphate. The antifungal metabolites inhibited mycelial growth of many species of Aspergillus, Penicillium and Fusarium. They also inhibited production of aflat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00431.x
更新日期:1998-06-01 00:00:00
abstract::A Gram-positive rod was isolated from a commercial grape wine undergoing a sluggish/stuck alcoholic fermentation. The organism produced L-lactic acid from glucose, possessed weak catalase activity, and fermented relatively few carbohydrates, i.e. glucose, fructose, sucrose, raffinose (weakly) and mannitol. The 16S rRN...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00399.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. METHODS AND RESULTS:Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A21...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14548
更新日期:2020-04-01 00:00:00
abstract:AIMS:To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS:The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campyloba...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02616.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04411.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:The objective of this study was to determine the technological behaviour (implantation and biogenic amines production) of Carnobacterium divergens V41, an anti-Listeria bacteriocin producer (divercin V41), after inoculation in cold smoked salmon (CSS). METHODS AND RESULTS:Implantation of the strain was followed b...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01561.x
更新日期:2002-01-01 00:00:00
abstract::The classification of Bacillus thuringiensis strains has been revised and updated based on flagellar antigens which have been in use for many years. Sixty-nine serotypes and 13 sub-antigenic groups have now been identified, giving 82 serovars among the 3500 B. thuringiensis isolates of the IEBC Collection. The number ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00710.x
更新日期:1999-04-01 00:00:00
abstract:AIMS:The aim of this study was the molecular identification of Salmonella enterica serovar Infantis (S. Infantis) isolated from poultry samples and their antimicrobial resistance and virulence profiling. METHODS AND RESULTS:A total of 149 isolates, belonging to genus Salmonella, originally isolated from 340 suspected ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14282
更新日期:2019-07-01 00:00:00
abstract::The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01189.x
更新日期:2000-11-01 00:00:00
abstract:AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03617.x
更新日期:2008-03-01 00:00:00
abstract:AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03386.x
更新日期:2007-11-01 00:00:00
abstract:AIM:A new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO(3) and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. METHODS AND RESULTS:Two evaluations of EC-Blue-10 were carried out. Firstly, EC-Blue-10 w...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03627.x
更新日期:2008-04-01 00:00:00
abstract:AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features simil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01651.x
更新日期:2002-01-01 00:00:00