Abstract：
AIMS:To evaluate a standard aerosolization method for uniformly depositing threat-representative spores onto surfaces. METHODS AND RESULTS:Lyophilized Bacillus anthracis ΔSterne spores, coated in silica, were aerosolized into a containment chamber and deposited onto nine surface types by two independent laboratories. Laboratory A produced a mean loading concentration of 1·78 × 10(5) CFU cm(-2) ; coefficient of variation (CV) was <40% for 96% of samples. Laboratory B produced a mean loading concentration of 7·82 × 10(6) CFU cm(-2) ; 68% of samples demonstrated CV <40%. CONCLUSIONS:This method has been shown to meet the goal of loading threat-representative spores onto surfaces with low variability at concentrations relevant to the Department of Defense. SIGNIFICANCE AND IMPACT OF THE STUDY:As demonstrated in 2001, a biological attack using anthrax disseminated as a dry powder is a credible threat. This method will provide a means to load spores onto surfaces that mimic a 'real-world' scenario of an aerosolized anthrax attack. The method has utility for evaluating sporicidal technologies and for nondecontamination studies, for example fate and transport or reaerosolization.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Harnish D,Heimbuch BK,McDonald M,Kinney K,Dion M,Stote R,Rastogi V,Smith L,Wallace L,Lumley A,Schreuder-Gibson H,Wander JDdoi
10.1111/jam.12509subject
Has Abstractpub_date
2014-07-01 00:00:00pages
40-9issue
1eissn
1364-5072issn
1365-2672journal_volume
117pub_type
杂志文章abstract：AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12960
更新日期：2015-12-01 00:00:00
abstract：:Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.13207
更新日期：2016-09-01 00:00:00
abstract：AIMS:We aimed at expressing heterologous proteins in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees, as a prerequisite for future studies on the molecular pathogenesis of P. larvae infections. METHODS AND RESULTS:For this purpose, we established a protocol for the transformation of the p...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.05214.x
更新日期：2012-03-01 00:00:00
abstract：AIMS:This study aimed at characterizing the lactic acid bacteria microbiota and selecting mixed endogenous starters to be used for sourdough fermentation of spelt or emmer flours. METHODS AND RESULTS:Identification of lactic acid bacteria was carried out by partial sequencing of the 16S rRNA, recA, 16S/23S rRNA spacer...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04497.x
更新日期：2010-03-01 00:00:00
abstract：AIMS:To compare survival of enterohaemorrhagic Escherichia coli (EHEC) strains of two clonal groups in a simulated gastric environment and to quantify the effect of storage in an acidic food, apple juice, on subsequent survival of EHEC in the simulated gastric environment. METHODS AND RESULTS:To characterize acid resi...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03099.x
更新日期：2007-02-01 00:00:00
abstract：AIMS:The fate of Listeria monocytogenes Scott A, was studied in broth, at different a(w)s (by adding NaCl or KCl from 0.0 to 1.4 mol l(-1)), pHs (from 4.0 to 7.3 by adding lactic acid), and nisin concentrations (from 0 to 100 IU ml(-1)). METHODS AND RESULTS:Increasing salt and nisin concentrations and decreasing pH re...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.03117.x
更新日期：2007-03-01 00:00:00
abstract：AIMS:To devise a protocol for heterologous expression and purification of a partial toxic portion of the Bacillus thuringiensis (Bt) vegetative insecticidal protein Vip3A and using it as an antigen for anti-Vip3A polyclonal antibody development. Also, to evaluate the regulation of Vip3A secretion into culture supernata...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13775
更新日期：2018-08-01 00:00:00
abstract：AIMS:To study the expression of cellular antigens of Listeria monocytogenes that react with monoclonal antibodies (MAbs) C11E9 and EM-7G1 under acid-, salt- or temperature-induced stress environments. METHODS AND RESULTS:The reaction patterns of antibodies to L. monocytogenes held in stressful environments for a short...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.02035.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:This study aimed to explore potential drug targets of Streptococcus suis at the system level. METHODS AND RESULTS:A homologous protein mapping method was used in the construction of a protein-protein interaction (PPI) network of S. suis, which presented 1147 nonredundant interaction pairs among 286 proteins. The ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14950
更新日期：2020-11-29 00:00:00
abstract：:A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00412.x
更新日期：1998-05-01 00:00:00
abstract：AIMS:"Soppressata molisana", a fermented sausage produced in southern Italy, is commonly obtained without starter addition. However, the use of starter cultures is more and more recommended in meat fermentation processes in order to guarantee stable production performance. In this study, the survival of the Staphylococ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2002.01512.x
更新日期：2002-01-01 00:00:00
abstract：:The enzymatic activity and viability of Escherichia coli O157:H7 in natural river water was determined by flow cytometry. River water was collected at two sites (an agricultural area and an industrial area) on the Aigawa River (Osaka, Japan). To facilitate estimation of the physiology of E. coli O157 in natural river ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.00960.x
更新日期：2000-02-01 00:00:00
abstract：AIMS:To study the behaviour of salmonellae inoculated in the dry, raw materials, related to chocolate confectionery manufacture, as affected by the strain, cell preparation method and storage temperature. METHODS AND RESULTS:Outbreak and non outbreak-associated salmonellae were used for the inoculation of cocoa butter...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04144.x
更新日期：2009-06-01 00:00:00
abstract：AIMS:To establish a laboratory model to compare the effectiveness of detergent-based disinfection procedures for reducing cross-contamination risks during handling of contaminated chicken. METHODS AND RESULTS:During handling of chickens, artificially contaminated with Salmonella enteritidis PT4, the organism was widel...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2003.02127.x
更新日期：2003-01-01 00:00:00
abstract：AIMS:To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. METHODS AND RESULTS:After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated Bacillu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2012.05395.x
更新日期：2012-10-01 00:00:00
abstract：AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03658.x
更新日期：2008-05-01 00:00:00
abstract：AIMS:To monitor emissions of NH(3) and N(2)O during composting and link these to ammonia oxidation rates and the community structure of ammonia oxidizing bacteria (AOB). METHODS AND RESULTS:A laboratory-scale compost reactor treating organic household waste was run for 2 months. NH(3) emissions peaked when pH started ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04111.x
更新日期：2009-05-01 00:00:00
abstract：AIMS:Microbial community associated with hydrogen production and volatile fatty acids (VFAs) accumulation was characterized in acidogenic hydrogenesis using molasses wastewater as a feedstock. METHODS AND RESULTS:Hydrogen and VFAs production were measured under an organic loading rate (OLR) from 19 to 35 g-COD l-1 da...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13316
更新日期：2016-12-01 00:00:00
abstract：AIMS:The isolation and partial characterization of anti-Listeria bacteriocin producing strains present in milk from areas of northern Greece in view of their potential use as protective cultures in food fermentations. METHODS AND RESULTS:Three hundred and thirty-two isolates were obtained from milk samples intended fo...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04932.x
更新日期：2011-03-01 00:00:00
abstract：AIMS:To investigate the factors affecting expression and solubilization of Escherichia coli maltodextrin glucosidase in E. coli. METHODS AND RESULTS:Expression level and solubilization of the recombinant E. coli maltodextrin glucosidase was studied in E. coli at different temperatures, in presence of overexpressed Gro...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03519.x
更新日期：2008-01-01 00:00:00
abstract：:The study of wild strains from natural habitats is a useful means of understanding better the heterogeneity within a species of biotechnological importance, and of obtaining atypical isolates with unknown capabilities. In the present research carried out on different Lactobacillus helveticus strains isolated from natu...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1997.00312.x
更新日期：1998-01-01 00:00:00
abstract：AIMS:To investigate the effects of ohmic heating (OH) and water bath heating (WB) on the membrane permeability, membrane structure, intracellular organization and leakage of intracellular substances of Escherichia coli O157:H7 at the same inactivation level and at a heating temperature of 72°C. METHODS AND RESULTS:Flo...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14411
更新日期：2019-11-01 00:00:00
abstract：:Cancer is a prominent cause of morbidity and mortality worldwide, in spite of advances in therapeutic interventions and supportive care. In 2018 alone, there were 18·1 million new cancer cases and 9·6 million deaths indicating the need for novel anticancer agents. Plant-based products have often been linked with prote...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/jam.14981
更新日期：2020-12-23 00:00:00
abstract：AIMS:Changes in fermentation pattern during the treatment of organic wastes containing solid materials by thermophilic anaerobic microflora were investigated with respect to product formation and bacterial community structure during hydrogen production. METHODS AND RESULTS:Anaerobic microflora enriched from sludge com...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02939.x
更新日期：2006-08-01 00:00:00
abstract：AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13309
更新日期：2016-12-01 00:00:00
abstract：AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02491.x
更新日期：2005-01-01 00:00:00
abstract：AIMS:To evaluate ATP bioluminescence-based hygiene monitoring systems under conditions relevant to fish processing environments. METHODS AND RESULTS:The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14292
更新日期：2019-07-01 00:00:00
abstract：AIMS:To determine whether isolates of Listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. METHODS AND RESULTS:Strains were assessed for their ability to adsorb to a test surface over a short time period. Although some differences in numbers of bound cells...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01419.x
更新日期：2001-10-01 00:00:00
abstract：AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2006.02989.x
更新日期：2006-07-01 00:00:00
abstract：AIMS:To investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. METHODS AND RESULTS:Culturable bacteria were isolated from buried tyre shreds and identified using fatty acid methyl ester analysis. Isolates belonged to taxonomic gr...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04157.x
更新日期：2009-06-01 00:00:00
