Immunodetection of the toxic portion of Vip3A reveals differential temporal regulation of its secretion among Bacillus thuringiensis strains.

abstract：AIMS:To enhance the recovery of f(2) bacteriophage and poliovirus by an established method based on the adsorption to and elution from positively-charged Al(OH)(3)-treated silica gel. METHODS AND RESULTS:Polyaluminum Chloride (PAC) was added to water samples to neutralize the negatively charged materials, which can re...

journal_title：Journal of applied microbiology

authors： Zhang X,Zhao Z,Shen Z,Wang XW,Guo J,Jin M,Wang J,Qiu Z,Li JW

更新日期：2009-02-01 00:00:00

abstract：AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...

journal_title：Journal of applied microbiology

authors： Martínez JM,Gómez EV,Pemán J,Cantón E,García MG,del Castillo Agudo L

更新日期：2010-06-01 00:00:00

abstract：AIMS:The metabolism of phenanthrene and anthracene by a moderate thermophilic Nocardia otitidiscaviarum strain TSH1 was examined. METHODS AND RESULTS:When strain TSH1 was grown in the presence of anthracene, four metabolites were identified as 1,2-dihydroxy-1,2-dihydroanthracene, 3-(2-carboxyvinyl)naphthalene-2-carbox...

journal_title：Journal of applied microbiology

authors： Zeinali M,Vossoughi M,Ardestani SK

更新日期：2008-08-01 00:00:00

abstract：AIMS:Bacillus anthracis strains of various origins were analysed with the view to describe intrinsic and persistent structural components of the Bacillus collagen-like protein of anthracis glycoprotein associated anthrose containing tetrasaccharide in the exosporium. METHODS AND RESULTS:The tetrasaccharide consists of...

journal_title：Journal of applied microbiology

authors： Tamborrini M,Oberli MA,Werz DB,Schürch N,Frey J,Seeberger PH,Pluschke G

更新日期：2009-05-01 00:00:00

abstract：AIMS:We aimed at expressing heterologous proteins in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees, as a prerequisite for future studies on the molecular pathogenesis of P. larvae infections. METHODS AND RESULTS:For this purpose, we established a protocol for the transformation of the p...

journal_title：Journal of applied microbiology

authors： Poppinga L,Genersch E

更新日期：2012-03-01 00:00:00

abstract：:The survival and environmental spread of Salmonella bacteria from domestic toilets was examined in homes, where a family member had recently suffered an attack of salmonellosis. In four out of six households tested, Salmonella bacteria persisted in the biofilm material found under the recess of the toilet bowl rim whi...

journal_title：Journal of applied microbiology

authors： Barker J,Bloomfield SF

更新日期：2000-07-01 00:00:00

abstract：AIM:This study was performed to isolate and characterize novel antifungal lipopeptide from Bacillus cereus. METHODS AND RESULTS:Elucidation of its chemical structure was carried out by electrospray ionization mass spectra (ESI-MS) and Fourier transform infrared spectroscopy (FT-IR). The compound is a cyclic heptapepti...

journal_title：Journal of applied microbiology

authors： Ajesh K,Sudarslal S,Arunan C,Sreejith K

更新日期：2013-12-01 00:00:00

abstract：AIMS:To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS:Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amende...

journal_title：Journal of applied microbiology

authors： Fremaux B,Prigent-Combaret C,Delignette-Muller ML,Mallen B,Dothal M,Gleizal A,Vernozy-Rozand C

更新日期：2008-01-01 00:00:00

abstract：AIMS:We propose and test an efficient and rapid protocol for the detection of toxigenic Fusarium isolates producing three main types of Fusarium-associated mycotoxins (fumonisins, trichothecenes and zearelanone). METHODS AND RESULTS:The novel approach utilizes partially multiplexed markers based on genes essential for...

journal_title：Journal of applied microbiology

authors： Dawidziuk A,Koczyk G,Popiel D,Kaczmarek J,Buśko M

更新日期：2014-06-01 00:00:00

abstract：AIMS:Algae are favourable as a biofuel source because of the potential high oil content and fast generation of biomass. However, one of the challenges for this technology is achieving high oil content while maintaining exponential or high growth of the organism. Introducing a two-stage reactor to optimize both growth a...

journal_title：Journal of applied microbiology

authors： Csavina JL,Stuart BJ,Riefler RG,Vis ML

更新日期：2011-08-01 00:00:00

abstract：AIMS:A Portable Multi-use Automated Concentration System (PMACS) concentrates micro-organisms from large volumes of water through automated dead-end ultrafiltration and backflushing. The ability to detect microbial targets from ground, surface and cooling tower waters collected using standard methods was compared with ...

journal_title：Journal of applied microbiology

authors： Leskinen SD,Kearns EA,Jones WL,Miller RS,Bevitas CR,Kingsley MT,Brigmon RL,Lim DV

更新日期：2012-08-01 00:00:00

abstract：AIMS:To assess the behaviour of Aspergillus section Nigri IOC 4573 in mango nectar as affected by temperature and pH. METHODS AND RESULTS:A central composite design (2(2)+2*2+3) was used to estimate the influence of temperature (17·2-22·8°C) and pH (3·28-4·7) on A. section Nigri growth (λ, lag time; μ, growth rate). S...

journal_title：Journal of applied microbiology

authors： Silva AR,Sant'Ana AS,Massaguer PR

更新日期：2010-09-01 00:00:00

abstract：AIMS:A novel alginate oligomer (OligoG CF-5/20) has been shown to potentiate antifungal therapy against a range of fungal pathogens. The current study assessed the effect of this oligomer on in vitro virulence factor expression and epithelial invasion by Candida species. METHODS AND RESULTS:Plate substrate assays and ...

journal_title：Journal of applied microbiology

authors： Pritchard MF,Jack AA,Powell LC,Sadh H,Rye PD,Hill KE,Thomas DW

更新日期：2017-09-01 00:00:00

abstract：AIMS:To determine the effect of antimicrobial selective pressure on the transmission of antimicrobial resistant and sensitive strains of Salmonella in poultry. METHODS AND RESULTS:Eight pens housed 12 broiler chicks each. Two chicks in four of the pens were inoculated with a Salm. Typhimurium strain resistant to 12 an...

journal_title：Journal of applied microbiology

authors： Bauer-Garland J,Frye JG,Gray JT,Berrang ME,Harrison MA,Fedorka-Cray PJ

更新日期：2006-12-01 00:00:00

abstract：AIMS:Lactococcus lactis N8 and Saccharomyces boulardii SAA655 were investigated for their ability to synthesize B-vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. METHODS AND RESULTS:In this study, ultra-high performance liquid chromatography and microbiological as...

journal_title：Journal of applied microbiology

authors： Chandrasekar Rajendran SC,Chamlagain B,Kariluoto S,Piironen V,Saris PEJ

更新日期：2017-06-01 00:00:00

abstract：AIMS:Investigation of waste pulps and soda pulp bleaching with xylanase (X) and laccase mediator system (LMS) alone and in conjunction (one after the other) (XLMS). METHODS AND RESULTS:Soda and different grades of waste pulp fibres [used for making three-layered duplex sheets - top layer (TL), protective layer (PL) an...

journal_title：Journal of applied microbiology

authors： Kapoor M,Kapoor RK,Kuhad RC

更新日期：2007-08-01 00:00:00

abstract：:The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...

journal_title：Journal of applied microbiology

authors： Méchin L,Dubois-Brissonnet F,Heyd B,Leveau JY

更新日期：1999-05-01 00:00:00

abstract：AIMS:To determine roles of cortex lytic enzymes (CLEs) in Bacillus megaterium spore germination. METHODS AND RESULTS:Genes for B. megaterium CLEs CwlJ and SleB were inactivated and effects of loss of one or both on germination were assessed. Loss of CwlJ or SleB did not prevent completion of germination with agents th...

journal_title：Journal of applied microbiology

authors： Setlow B,Peng L,Loshon CA,Li YQ,Christie G,Setlow P

更新日期：2009-07-01 00:00:00

abstract：AIMS:Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. METHODS AND RESULTS:Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity p...

journal_title：Journal of applied microbiology

authors： Daba GM,Ishibashi N,Zendo T,Sonomoto K

更新日期：2017-11-01 00:00:00

abstract：AIMS:To determine how hydrated Bacillus anthracis spores are killed in a high-temperature gas environment (HTGE), and how spores of several Bacillus species including B. anthracis are killed by UV radiation, dry heat, wet heat and desiccation. METHODS AND RESULTS:Hydrated B. anthracis spores were HTGE treated at c. 22...

journal_title：Journal of applied microbiology

authors： Setlow B,Parish S,Zhang P,Li YQ,Neely WC,Setlow P

更新日期：2014-04-01 00:00:00

abstract：AIMS:The present study aimed to determine the microsatellite length polymorphism (MLP) genotypic patterns and antifungal susceptibility profiles of Candida albicans isolated from patients with candiduria. METHODS AND RESULTS:DNA of 50 C. albicans isolates was used for molecular identification based on the ITS1 -5.8s-I...

journal_title：Journal of applied microbiology

authors： Gharaghani M,Rezaei-Matehkolaei A,Hardani AK,Zarei Mahmoudabadi A

更新日期：2021-01-18 00:00:00

abstract：AIM:This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. METHODS AND RESULTS:Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains ...

journal_title：Journal of applied microbiology

authors： dos Santos SG,Diniz CG,da Silva VL,Souza NC,de Lima FL,Bomfim MR,de Carvalho MA,Farias LM

更新日期：2007-10-01 00:00:00

abstract：:Sustainable economic and industrial growth is the need of the hour and it requires renewable energy resources having better performance and compatibility with existing fuel infrastructure from biological routes. Isoprenoids (C ≥ 5) can be a potential alternative due to their diverse nature and physiochemical propertie...

journal_title：Journal of applied microbiology

authors： Gupta P,Phulara SC

更新日期：2015-09-01 00:00:00

abstract：AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...

journal_title：Journal of applied microbiology

authors： Wang L,Li Y,Mustapha A

更新日期：2009-11-01 00:00:00

abstract：:An extracellular phenolic acid esterase produced by the fungus Penicillium expansum in solid state culture released ferulic and rho-coumaric acid from methyl esters of the acids, and from the phenolic-carbohydrate esters O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl]-(1-->3)-O-beta- D-xylopyranosyl-(1-->4)-D-xylopy...

journal_title：Journal of applied microbiology

authors： Donaghy J,McKay AM

更新日期：1997-12-01 00:00:00

abstract：AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...

journal_title：Journal of applied microbiology

authors： Montanari G,Borsari A,Chiavari C,Ferri G,Zambonelli C,Grazia L

更新日期：2004-01-01 00:00:00

abstract：AIMS:The aims of this study were to determine the occurrence of Fusarium graminearum species complex (FGSC) on soybean pods, seeds and roots, including rhizoplane, during the period of soybean crop in rotation with wheat and to evaluate the FGSC dynamics on wheat and soybean residues during two soybean growing seasons ...

journal_title：Journal of applied microbiology

authors： Chiotta ML,Alaniz Zanon MS,Palazzini JM,Alberione E,Barros GG,Chulze SN

更新日期：2021-01-01 00:00:00

abstract：AIMS:Phenotypic and genotypic bacteria identification methods were compared for their efficacy in determining the composition of competitive exclusion (CE) products. METHODS AND RESULTS:Phenotypic methods used for bacterial identification were fatty acid methyl ester profiles, biochemical assays and carbohydrate utili...

journal_title：Journal of applied microbiology

authors： Wagner RD,Paine DD,Cerniglia CE

更新日期：2003-01-01 00:00:00

abstract：AIMS:In this work, the 'hi-tech' complex biocleaning and restoration of the 14th-century fresco Triumph of Death (5·6 × 15·0 m) at the Camposanto Monumental Cemetery (Pisa, Italy) is reported. Since 2000, the restoration based on the biological cleaning of noble medieval frescoes, has been successfully utilized in this...

journal_title：Journal of applied microbiology

authors： Ranalli G,Zanardini E,Andreotti A,Colombini MP,Corti C,Bosch-Roig P,De Nuntiis P,Lustrato G,Mandrioli P,Rampazzi L,Giantomassi C,Zari D

更新日期：2018-09-01 00:00:00

abstract：AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...

journal_title：Journal of applied microbiology

authors： Horakova K,Mlejnkova H,Mlejnek P

更新日期：2008-10-01 00:00:00