Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase.

abstract：AIM:The objective of this study was to evaluate the probiotic potential of lactic acid bacteria (LAB) isolated from the intestinal ecosystem of rainbow trout. METHODS AND RESULTS:Among LAB isolates, 10 of them were selected and screened for resistance to acid and bile salts, pancreatin, sodium chloride and temperature...

journal_title：Journal of applied microbiology

authors： Mortezaei F,Royan M,Allaf Noveirian H,Babakhani A,Alaie Kordghashlaghi H,Balcázar JL

更新日期：2020-10-01 00:00:00

abstract：AIMS:To evaluate four DNA extraction methods to elucidate the most effective method for bacterial DNA recovery from human milk (HM). METHODS AND RESULTS:Human milk DNA was extracted using the following methods: (i) Qiagen MagAttract Microbial DNA Isolation Kit (kit QM), (ii) Norgen Milk Bacterial DNA Isolation Kit (ki...

journal_title：Journal of applied microbiology

authors： Cheema AS,Stinson LF,Lai CT,Geddes DT,Payne MS

更新日期：2021-01-01 00:00:00

abstract：AIM:To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. METHODS AND RESULTS:T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1). Suspension of T. scotoductus SA-01 cells also reduced Cr(VI) aero...

journal_title：Journal of applied microbiology

authors： Opperman DJ,van Heerden E

更新日期：2007-11-01 00:00:00

abstract：AIMS:The major aims of this study are to determine the capability of sulphur oxidizing bacterium (SOB-1) to desulphurize dibenzothiophene (DBT) and crude oil, detection of the reaction kinetics and identify the proposed pathway of DBT desulphurization. METHODS AND RESULTS:The isolate was genetically identified based o...

journal_title：Journal of applied microbiology

authors： Mawad AMM,Hassanein M,Aldaby ES,Yousef N

更新日期：2020-08-19 00:00:00

abstract：AIMS:To evaluate the use of relatively low levels of hydrogen peroxide vapour (HPV) for the inactivation of Bacillus anthracis spores within an indoor environment. METHODS AND RESULTS:Laboratory-scale decontamination tests were conducted using bacterial spores of both B. anthracis Ames and Bacillus atrophaeus inoculat...

journal_title：Journal of applied microbiology

authors： Wood JP,Calfee MW,Clayton M,Griffin-Gatchalian N,Touati A,Ryan S,Mickelsen L,Smith L,Rastogi V

更新日期：2016-12-01 00:00:00

abstract：AIMS:To isolate an iron-reducing bacterium and examine its ability of Fe(III) oxide reduction and dechlorination. METHODS AND RESULTS:A fermentative facultative anaerobe, strain L17 isolated from subterranean sediment, can reduce Fe(III) oxides and carbon tetrachloride (CT). It was identified as Klebsiella pneumoniae ...

journal_title：Journal of applied microbiology

authors： Li XM,Zhou SG,Li FB,Wu CY,Zhuang L,Xu W,Liu L

更新日期：2009-01-01 00:00:00

abstract：AIM:Shiga toxin-producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by the availability of sensitive methods for detecting these pathogens. In this study, we developed novel too...

journal_title：Journal of applied microbiology

authors： Kirchner M,Sayers E,Cawthraw S,Duggett N,Gosling R,Jenkins C,Dallman TJ,Mueller-Doblies D,Anjum MF

更新日期：2019-09-01 00:00:00

abstract：AIMS:This study aimed at evaluating the inhibitory effects of various organic extracts of Ocimum basilicum against some species of Bipolaris and Cochliobolus with GC-MS and HPLC analysis. METHODS AND RESULTS:The ethyl acetate extract consisted of methyl cinnamate as the most abundant component, while butylated hydroxy...

journal_title：Journal of applied microbiology

authors： Elsherbiny EA,Safwat NA,Elaasser MM

更新日期：2017-10-01 00:00:00

abstract：AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...

journal_title：Journal of applied microbiology

authors： Asvapathanagul P,Olson BH

更新日期：2017-01-01 00:00:00

abstract：AIM:To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8. METHODS AND RESULTS:DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richnes...

journal_title：Journal of applied microbiology

authors： García Bernal M,Trabal Fernández N,Saucedo Lastra PE,Medina Marrero R,Mazón-Suástegui JM

更新日期：2017-03-01 00:00:00

abstract：:Currently, symbiotic rhizobia (sl., rhizobium) refer to the soil bacteria in α- and β-Proteobacteria that can induce root and/or stem nodules on some legumes and a few of nonlegumes. In the nodules, rhizobia convert the inert dinitrogen gas (N2 ) into ammonia (NH3 ) and supply them as nitrogen nutrient to the host pla...

journal_title：Journal of applied microbiology

authors： Chen WF,Wang ET,Ji ZJ,Zhang JJ

更新日期：2020-12-10 00:00:00

abstract：AIMS:There is emerging evidence that point-of-use (POU) water treatment can be effective in the improvement of microbial water quality and the decrease in diarrhoeal disease incidence in humanitarian emergencies. Coagulant/disinfection products (CDPs) have the distinct advantage of providing microbial quality improveme...

journal_title：Journal of applied microbiology

authors： Marois-Fiset JT,Shaheed A,Brown J,Dorea CC

更新日期：2016-09-01 00:00:00

abstract：AIM:To evaluate the antifungal activity of extracts of Chamaecostus cuspidatus against Candida and Trichophyton species. METHODS AND RESULTS:Crude ethanol extracts of leaves, stems and rhizomes were prepared and evaluated for antimicrobial activity. Only the rhizomes extract (RE) showed antifungal activity but had no ...

journal_title：Journal of applied microbiology

authors： Barros Cota B,Batista Carneiro de Oliveira D,Carla Borges T,Cristina Catto A,Valverde Serafim C,Rogelis Aquiles Rodrigues A,Kohlhoff M,Leomar Zani C,Assunção Andrade A

更新日期：2021-01-01 00:00:00

abstract：AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences...

journal_title：Journal of applied microbiology

authors： Botti S,Bertaccini A

更新日期：2003-01-01 00:00:00

abstract：AIMS:The cell-surface display of chitinase Chi9602ΔSP was constructed on Bacillus thuringiensis BMB171 by using two repeat N-terminal regions of autolysin (Mbgn)2 as the anchoring motif. This study aimed to prepare a chitinolytic whole-cell B. thuringiensis biocatalyst with improved catalytic and antifungal activities....

journal_title：Journal of applied microbiology

authors： Tang M,Sun X,Zhang S,Wan J,Li L,Ni H

更新日期：2017-01-01 00:00:00

abstract：AIM:To investigate the effect of corn starch (CS) edible films with spice oils on the stability of raw beef during refrigerated storage. METHODS AND RESULTS:The antimicrobial properties of corn starch films containing 0-4·0% (w/v) ratios of clove (CL) and cinnamon (CI) essential oils (EOs) were tested against seven me...

journal_title：Journal of applied microbiology

authors： Radha Krishnan K,Babuskin S,Rakhavan KR,Tharavin R,Azhagu Saravana Babu P,Sivarajan M,Sukumar M

更新日期：2015-12-01 00:00:00

abstract：AIMS:Growth of the lactic acid bacterium (LAB) Oenococcus oeni, which is involved in malolactic fermentation during the winemaking process, is stimulated by peptides originating from yeast. In this study, we investigated the impact of peptides on O. oeni growth, peptidase activity and the expression of genes encoding t...

journal_title：Journal of applied microbiology

authors： Ritt JF,Remize F,Grandvalet C,Guzzo J,Atlan D,Alexandre H

更新日期：2009-03-01 00:00:00

abstract：:The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...

journal_title：Journal of applied microbiology

authors： Pascual S,De Cal A,Magan N,Melgarejo P

更新日期：2000-11-01 00:00:00

abstract：AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...

journal_title：Journal of applied microbiology

authors： Crowley KM,Prendergast DM,Sheridan JJ,McDowell DA

更新日期：2010-10-01 00:00:00

abstract：AIMS:Algae are favourable as a biofuel source because of the potential high oil content and fast generation of biomass. However, one of the challenges for this technology is achieving high oil content while maintaining exponential or high growth of the organism. Introducing a two-stage reactor to optimize both growth a...

journal_title：Journal of applied microbiology

authors： Csavina JL,Stuart BJ,Riefler RG,Vis ML

更新日期：2011-08-01 00:00:00

abstract：AIMS:To examine the involvement of N-acyl homoserine lactone (AHL) quorum sensing in alkaline metalloprotease (aprX) promoter regulation in a Pseudomonas fluorescens milk isolate. METHODS AND RESULTS:N-acyl homoserine lactone signals from P. fluorescens strain 395 were separated and detected by thin layer chromatograp...

journal_title：Journal of applied microbiology

authors： Liu M,Wang H,Griffiths MW

更新日期：2007-12-01 00:00:00

abstract：AIMS:To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS:The MPN method was a combination of a...

journal_title：Journal of applied microbiology

authors： Jenkins MB,Endale DM,Fisher DS

更新日期：2008-06-01 00:00:00

abstract：AIMS:Chlamydia trachomatis and herpes simplex virus (HSV) are the most prevalent bacterial and viral sexually transmitted infections. Due to the chronic nature of their infections, they are able to interact with titanium-dioxide (TiO2 ) nanoparticles (NPs) applied as food additives or drug delivery vehicles. The aim of...

journal_title：Journal of applied microbiology

authors： Bogdanov A,Janovák L,Lantos I,Endrész V,Sebők D,Szabó T,Dékány I,Deák J,Rázga Z,Burián K,Virok DP

更新日期：2017-11-01 00:00:00

abstract：:Application of high hydrostatic pressure (200, 300, 350 and 400 MPa) at 5 degrees C for 30 min to different micro-organisms, including Gram-positive and Gram-negative bacteria, moulds and yeasts, proved to be more effective in inactivating these organisms than treatments at 20 degrees C for 10 min and at 10 degrees C ...

journal_title：Journal of applied microbiology

authors： Arroyo G,Sanz PD,Préstamo G

更新日期：1999-03-01 00:00:00

abstract：:A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...

journal_title：Journal of applied microbiology

authors： McGuigan KG,Joyce TM,Conroy RM,Gillespie JB,Elmore-Meegan M

更新日期：1998-06-01 00:00:00

abstract：AIMS:To investigate the ecophysiology of populations of polyphosphate-accumulating organisms (PAO) and glycogen-accumulating organisms (GAO) in communities of a novel acetate fed process removing phosphate from wastewater. Attempts were made to see if acetate could be replaced by an alternative carbon source which did ...

journal_title：Journal of applied microbiology

authors： Schroeder S,Ahn J,Seviour RJ

更新日期：2008-11-01 00:00:00

abstract：AIMS:To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America. METHODS AND RESULTS:16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), mu...

journal_title：Journal of applied microbiology

authors： Clementino MM,Fernandes CC,Vieira RP,Cardoso AM,Polycarpo CR,Martins OB

更新日期：2007-07-01 00:00:00

abstract：AIMS:Poor colonization of the rhizosphere is a major constraint of seed treatment biological control. The objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribu...

journal_title：Journal of applied microbiology

authors： Walker R,Rossall S,Asher MJ

更新日期：2002-01-01 00:00:00

abstract：AIMS:Due to their antimicrobial activity, silver nanoparticles (Ag-NPs) are being increasingly used in a number of industrial products. The accumulation of Ag-NPs in the soil might affect plant growth-promoting rhizobacteria and, in turn, the plants. We describe the effects of Ag-NPs on the soil bacteria Azotobacter vi...

journal_title：Journal of applied microbiology

authors： Gambino M,Marzano V,Villa F,Vitali A,Vannini C,Landini P,Cappitelli F

更新日期：2015-05-01 00:00:00

abstract：AIMS:To characterize the non-spoilage variant obtained from beer-spoilage Lactobacillus brevis ABBC45C and to identify a potential genetic marker capable of discriminating beer-spoilage L. brevis strains from non-spoilers. METHODS AND RESULTS:A non-spoilage variant was obtained from beer-spoilage L. brevis ABBC45C by ...

journal_title：Journal of applied microbiology

authors： Suzuki K,Koyanagi M,Yamashita H

更新日期：2004-01-01 00:00:00