Abstract:
AIMS:The major aims of this study are to determine the capability of sulphur oxidizing bacterium (SOB-1) to desulphurize dibenzothiophene (DBT) and crude oil, detection of the reaction kinetics and identify the proposed pathway of DBT desulphurization. METHODS AND RESULTS:The isolate was genetically identified based on 16S rRNA gene sequencing as Klebsiella oxytoca and deposited in the Genebank database under the accession number: MT355440. The HPLC analysis of the remaining DBT concentration revealed that, SOB-1 could desulphurize 90% of DBT (0·25 mmol l-1 ) within 96 h. The maximum production of sulphate ions from the desulphurization of DBT (0·36 mmol l-1 ) and crude oil (0·4 mmol l-1 ) could be quantitatively detected after 48 h of incubation at 30°C. The high values of correlation coefficient (R2 ) obtained at all studied concentrations; suggested that biodesulfurization kinetics of DBT follows the first-order reaction model. The kinetics studies showed that, DBT may have an inhibitory effect on SOB-1 when the initial concentration exceeded 0·75 mmol l-1 . The GC-MS analysis exhibited four main metabolites rather than DBT. The most important ones are 2-hydroxybiphenyl (2-HBP) and methoxybiphenyl n(2-MBP). CONCLUSIONS:Klebsiella oxytoca SOB-1 catalyzes the desulphurization of DBT through 4S pathway and forms four main metabolic products. The release of sulphate ion and formation of 2-HBP indicating the elimination of sulphur group without altering the carbon skeleton of DBT. The bacterial strain could also catalyzes desulphurization of crude oil. The desulphurization kinetics follows the first-order reaction model. SIGNIFICANCE AND IMPACT OF THE STUDY:Klebsiella oxytoca SOB-1 could be used as a promising industrial and environmental biodesulfurizing agent as it is not affecting carbon skeleton of thiophenic compounds and forming less toxic metabolic product (2-MBP).
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Mawad AMM,Hassanein M,Aldaby ES,Yousef Ndoi
10.1111/jam.14829subject
Has Abstractpub_date
2020-08-19 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract:AIMS:This work focuses on the development of a method for the identification of pathogenic yeast. With this aim, we target the nucleotide sequence of the RPS0 gene of pathogenic yeast species with specific PCR primers. PCR analysis was performed with both the genomic DNA, whole cells of clinical isolates of Candida spe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04595.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:To determine whether the beer-spoilage ability is an intrinsic character of Lactobacillus paracollinoides and identify a genetic marker for differentiating the beer-spoilage ability of strains belonging to this species. METHODS AND RESULTS:The ribotype of a nonspoilage strain, Lact. brevis ATCC8291, was found to ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02350.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04561.x
更新日期:2010-04-01 00:00:00
abstract:AIMS:Campylobacter contamination in French chicken production from the farm to the consumer was determined using a PCR assay for bacteria detection and identification. METHODS AND RESULTS:Samples were bird droppings from poultry houses, neck skins, livers, hearts, gizzards, wings, legs and escalopes from slaughterhous...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01380.x
更新日期:2001-08-01 00:00:00
abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore resistance to and killing by a novel sporicide, dimethyldioxirane (DMDO) that was generated in situ from acetone and potassium peroxymonosulfate at neutral pH. METHODS AND RESULTS:Spores of B. subtilis were effectively killed by DMDO. Rates of killing by DMDO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03000.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:The present work aimed to obtain bioinsecticide over-producing mutants through classical mutagenesis of vegetative cells of Bacillus thuringiensis (Bt) by using u.v. and nitrous acid, and to evidence the involvement of cell metabolism in delta-endotoxin synthesis. METHODS AND RESULTS:Vegetative cells of Bt were t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02323.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04082.x
更新日期:2009-04-01 00:00:00
abstract:AIMS:To investigate the occurrence and extent of Saccharomyces cerevisiae and Oenococcus oeni interactions. METHODS AND RESULTS:Interactions between S. cerevisiae and O. oeni were investigated by double-layer and well-plate assays showing the occurrence of specific interactions for each yeast-malolactic bacteria (MLB)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02579.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The aims of this study were to develop a sensitive and more rapid detection of Propionibacterium acidipropionici DH42 in silage and rumen fluid samples, and to explore its 16S rRNA sequence-based phylogeny. METHODS AND RESULTS:Nested polymerase chain reaction (PCR) was used with DH42-specific primers dhb1 and dhb...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02282.x
更新日期:2004-01-01 00:00:00
abstract::New types of antimicrobial compounds were identified in the culture filtrate of Lactobacillus plantarum VTT E-78076. Activity was detected in the low molecular mass fraction separated by gel chromatography. This fraction totally inhibited the growth of the Gram-negative test organism, Pantoea agglomerans (Enterobacter...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00632.x
更新日期:1999-01-01 00:00:00
abstract:AIMS:The goal of this study was to develop and test the efficacy of a PCR assay for the environmental detection of the nifH gene of Methanobrevibacter smithii, a methanogen found in human faeces and sewage. METHODS AND RESULTS:PCR primers for the nifH gene of M. smithii were designed, tested and used to detect the pre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02989.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:The study was undertaken to investigate the occurrence of glutamate dehydrogenase activity in different species of lactobacilli, and to determine, in a series of cheese-making trials, the effects of glutamate dehydrogenase-producing adjunct cultures on sensory attribute development during the maturation of cheddar...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03017.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:To identify bacterial pathogens of diseased NiIe tilapia Oreochromis niloticus and determine their virulence. METHODS AND RESULTS:Sixteen bacterial isolates were recovered from diseased Nile tilapias (O. niloticus) reared in floating cages in Adolfo Lopez Mateos (ALM), Sanalona and Dique IV dams in Sinaloa, Mexic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12280
更新日期:2013-09-01 00:00:00
abstract::Bacterial isolates from bean-sprouts were screened for anti-Listeria monocytogenes bacteriocins using a well diffusion method. Thirty-four of 72 isolates inhibited the growth of L. monocytogenes Scott A. One, HPB 1688, which had the biggest inhibition zone against L. monocytogenes Scott A, was selected for subsequent ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00262.x
更新日期:1997-10-01 00:00:00
abstract:AIMS:To determine the differences in competitive fitness among Escherichia coli strains with different plasmid profiles when grown in suspension with commensal faecal bacteria from growing swine fed chlortetracycline-supplemented or unsupplemented diets. METHODS AND RESULTS:Five multiple drug-resistant (MDR) E. coli s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05365.x
更新日期:2012-09-01 00:00:00
abstract:AIMS:To assess the efficacy of a β-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. METHODS AND RESULTS:Three different doses (0.5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,随机对照试验
doi:10.1111/jam.12713
更新日期:2015-02-01 00:00:00
abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00412.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS:Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in bi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04508.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:To investigate the nitrogen-microbial community dynamics during composting of a mixture of nitrifying waste activated sludge (WAS) and fine organic fraction of residual household waste (RHW). To examine whether the addition of nitrifying sludge could promote ammonia oxidation and reduce ammonia emissions. METHODS...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12651
更新日期:2014-12-01 00:00:00
abstract:AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2004.02162.x
更新日期:2004-01-01 00:00:00
abstract::Self-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04024.x
更新日期:2009-04-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05296.x
更新日期:2012-06-01 00:00:00
abstract::An extracellular alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus woesei has been cloned and sequenced. The 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species P. furiosus. By using a shuttle cloning vector for halophilic bacteria,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00988.x
更新日期:2000-03-01 00:00:00
abstract:AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13309
更新日期:2016-12-01 00:00:00
abstract:AIMS:This study investigated whether the higher incidence of recovery from meat of Listeria innocua compared with L. monocytogenes could be due to the laboratory media used, leading to an artificially lower detection of the pathogenic species, L. monocytogenes. METHODS AND RESULTS:Minced beef was inoculated with L. mo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01336.x
更新日期:2001-06-01 00:00:00
abstract:AIM:To investigate the effect of corn starch (CS) edible films with spice oils on the stability of raw beef during refrigerated storage. METHODS AND RESULTS:The antimicrobial properties of corn starch films containing 0-4·0% (w/v) ratios of clove (CL) and cinnamon (CI) essential oils (EOs) were tested against seven me...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12932
更新日期:2015-12-01 00:00:00
abstract::Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal salts medium and incubated at 25 degrees C for 48 h to allow production of protease. A zinc-metalloacid protease was purified from the cell-free concentrate by anion exchange and gel filtration chromatography. The purified proteas...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01108.x
更新日期:2000-08-01 00:00:00
abstract:AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) ,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04820.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14472
更新日期:2020-02-01 00:00:00
abstract:AIMS:To develop a real-time PCR assay targeting the Escherichia coli flagellar antigen H21 for identification and surveillance of clinically important Shiga toxin-producing E. coli (STEC) serotypes classified in seropathotype C. METHODS AND RESULTS:The fliC allele of STEC O91:H21 strain B2F1 was amplified and sequence...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03607.x
更新日期:2008-03-01 00:00:00