Abstract:
AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) , 0·95, 0·90 and 0·85) for 6 weeks. METHODS AND RESULTS:Real-time PCR was used to amplify the nor-1 gene (target gene), and benA56 (β-tubulin gene) used as a control gene. Expression of three structural genes, nor-1 (=aflD), ver-1 (=aflM), and omtA (=aflP), and the regulatory gene aflR of the aflatoxin biosynthetic pathway were also assayed. There were significant differences between nor-1 gene expression at the three a(w) levels; higher expression at 0·90 a(w) in weeks 1-3, when compared to 0·95. In contrast, in the driest treatment (0·85 a(w) ) none or very low nor-1 expression occurred. The populations of A. flavus colony-forming units (CFUs g(-1) ) increased over time with the highest at 0·95 a(w) . Highest AFB(1) production was at 0·90 and 0·95 a(w) from weeks 3-6. A(w) had a significant effect on aflR transcription at 0·95 a(w) over the 6-week period, while at 0·90 a(w) , only in the last 2 weeks. CONCLUSIONS: Correlations between different factors showed that log AFB(1) × log CFUs, log AFB(1) × a(w) , and log CFUs × a(w) were statistically significant, while log CFUs × RQ-PCR and RQ-PCR × a(w) were not. The AflR gene may not have an important role in the regulation of nor-1 expression in food matrices (e.g. peanuts). SIGNIFICANCE AND IMPACT OF THE STUDY:Determination of correlations between nor-1 expression and aflatoxin production by A. flavus in raw peanuts under different a(w) levels could be helpful to predict potential risk of aflatoxin production during storage of this hygroscopic food product and minimize contamination with the AFB(1) .
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Abdel-Hadi A,Carter D,Magan Ndoi
10.1111/j.1365-2672.2010.04820.xsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
1914-22issue
6eissn
1364-5072issn
1365-2672pii
JAM4820journal_volume
109pub_type
杂志文章abstract:AIMS:To identify the chemical structure of a bacteriocin, thermophilin 1277, produced by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277 was purified and partial N-terminal sequence analysis revealed 6 unidentified amino acids amongst 31 amino acids residues. A 2.7-kbp region containing the t...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2008.04059.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also a...
journal_title:Journal of applied microbiology
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更新日期:2010-08-01 00:00:00
abstract:AIMS:To contribute to the understanding of Cytisus scoparius success at invading and establishing itself in Australia. METHODS AND RESULTS:Root-nodule bacteria isolated from C. scoparius, growing on five different sites and originally introduced to Australia, were compared with isolates from indigenous plants growing ...
journal_title:Journal of applied microbiology
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更新日期:2006-06-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2010-01-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/jam.14770
更新日期:2021-01-01 00:00:00
abstract::Differences in opacity between wells of a microtitre plate containing different volumes of inoculated growth medium reflected planktonic growth without any contribution from cells attached at the well surface. Simple algebra and a knowledge of the dependence of optical density upon sample path length (volume) for susp...
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更新日期:1998-05-01 00:00:00
abstract::Additives for use in animal nutrition are regulated under Regulation (EC) No. 1831/2003. The scope of this paper addresses the specific microbiological issues relevant to a microbial feed additive, containing a Bacillus spp. and uses as an example a product with the trade name, Calsporin. Bacillus subtilis C-3102 is t...
journal_title:Journal of applied microbiology
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更新日期:2006-09-01 00:00:00
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更新日期:2014-12-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02385.x
更新日期:2004-01-01 00:00:00
abstract::Two abundant surface proteins, EA1 and Sap, are components of the Bacillus anthracis surface layer (S-layer). Their corresponding genes have been cloned, shown to be clustered on the chromosome and sequenced. EA1 and Sap each possess three 'S-layer homology' motifs. Single and double disrupted mutants were constructed...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00882.x
更新日期:1999-08-01 00:00:00
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更新日期:2017-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02923.x
更新日期:2006-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13775
更新日期:2018-08-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1111/jam.15007
更新日期:2021-01-18 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00134.x
更新日期:1997-04-01 00:00:00
abstract::With the emergence of antibiotic resistance, the interest for antimicrobial agents has recently increased again in public health. Copper was recognized in 2008 by the United States Environmental Protection Agency (EPA) as the first metallic antimicrobial agent. This led to many investigations of the various properties...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13681
更新日期:2018-05-01 00:00:00
abstract:AIMS:This meta-analysis aims to assess the effect of lactic acid bacteria (LAB) inoculation on fermentation parameters, microbiological composition and aerobic stability of corn silage. METHODS AND RESULTS:Databases (PubMed, ScienceDirect and Scopus) were searched from 1980 to 2017. The criteria for inclusion were: ra...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.14084
更新日期:2018-08-24 00:00:00
abstract:AIMS:The production of putrescine is a relevant property related to food quality and safety. Morganella morganii is responsible for putrescine production in fresh fish decomposition. The aim of this study was to gain deeper insights into the genetic determinants for putrescine production in M. morganii. METHODS AND RE...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.03188.x
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abstract:AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2007.03617.x
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abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title:Journal of applied microbiology
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更新日期:2020-03-01 00:00:00
abstract:AIM:Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, Sitophilus oryzae, Sitophilus zeamais, Tribolium confusum and Tribolium castaneum to elucidate if they can be the origin of Type I sourdough micro-organisms (Lactobacillus sanfrancisc...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.13546
更新日期:2017-10-01 00:00:00
abstract:AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04082.x
更新日期:2009-04-01 00:00:00
abstract::Due to the increasing number of nosocomial infections and multidrug-resistant bacterial strains, Staphylococcus aureus is now a major worldwide concern. Rapid detection and characterization of this bacterium has become an important issue for biomedical applications. Biosensors are increasingly appearing as low-cost, e...
journal_title:Journal of applied microbiology
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更新日期:2017-11-01 00:00:00
abstract:AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04591.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:To evaluate the performance of three Salmonella plating media (Rambach, Xylose Lysine Deoxycholate agar and modified Brilliant Green Agar plus Novobiocin) as part of the ISO 6579: 2002 (Annex D) on poultry environmental samples. METHODS AND RESULTS:The samples analysed were those for the European Union Salmonella...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04386.x
更新日期:2009-12-01 00:00:00
abstract:AIMS:To estimate the effect of pH on the structures of bacterial community during fermentation of vegetable wastes and to investigate the relationship between bacterial community dynamics and product distribution. METHODS AND RESULTS:The bacterial communities in five batch tests controlled at different pH values [unco...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03321.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:The objective of this study was to access APC-effector cell cluster formation in genetically susceptible BALB/c (H-2(d) ) mice infected with highly virulent Moscow strain of ectromelia virus (ECTV-MOS) and estimate of lymphocyte activation based upon expression of CD62L and CD44 molecules. METHODS AND RESULTS:APC...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04813.x
更新日期:2010-11-01 00:00:00
abstract:AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01767.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To evaluate the sensitivity and specificity of the BioFire Diagnostics FilmArray(®) system in combination with their Biothreat Panel for the detection of Bacillus anthracis (Ba), Francisella tularensis (Ft) and Yersinia pestis (Yp) DNA, and demonstrate the detection of Ba spores. METHODS AND RESULTS:DNA samples f...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12107
更新日期:2013-04-01 00:00:00
abstract:AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13309
更新日期:2016-12-01 00:00:00