Synergistic mode of action of catechin, vanillic and protocatechuic acids to inhibit the adhesion of uropathogenic Escherichia coli on silicone surfaces.

Abstract:

AIMS:To study the individual and combined contribution of catechin, protocatechuic and vanillic acids to inhibit the adhesion of uropathogenic Escherichia coli (UPEC) on the surface of silicone catheters. METHODS AND RESULTS:The adhesion of UPEC to silicone catheters during the exposure to nonlethal concentrations of phenolic compounds was measured, as well as changes in motility, presence of fimbriae, extra-cellular polymeric substances, surface charge, hydrophobicity and membrane fluidity. The phenolic combination reduced 26-51% of motility, 1 log CFU per cm2 of adhered bacteria and 20-40% the carbohydrate and protein content in the biofilm matrix. Curli fimbriae, surface charge and cell hydrophobicity were affected to a greater extent by the phenolic combination. In the mixture, vanillic acid was the most effective for reducing bacterial adhesion, extra-polymeric substance production, motility, curli fimbriae and biofilm structure. Notwithstanding, protocatechuic acid caused major changes in the bacterial cell surface properties, whereas catechin affected the cell membrane functionality. CONCLUSION:Catechin, protocatechuic and vanillic acids have different bacterial cell targets, explaining the synergistic effect of their combination against uropathogenic E. coli. SIGNIFICANCE AND IMPACT OF STUDY:This study shows the contribution of catechin, protocatechuic and vanillic acids in producing a synergistic mixture against the adhesion of uropathogenic E. coli on silicone catheters. The action of catechin, vanillic and protocatechuic acids included specific contributions of each compound against the E. coli membrane's integrity, motility, surface properties and production of extracellular polymeric substances. Therefore, the studied mixture of phenolic compounds could be used as an antibiotic alternative to reduce urinary tract infections associated with silicone catheters.

journal_name

J Appl Microbiol

authors

Bernal-Mercado AT,Gutierrez-Pacheco MM,Encinas-Basurto D,Mata-Haro V,Lopez-Zavala AA,Islas-Osuna MA,Gonzalez-Aguilar GA,Ayala-Zavala JF

doi

10.1111/jam.14472

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

387-400

issue

2

eissn

1364-5072

issn

1365-2672

journal_volume

128

pub_type

杂志文章
  • The microstructure and distribution of micro-organisms within mature Serra cheese.

    abstract::The distribution of micro-organisms in mature Serra, a traditional Portuguese cheese made from unpasteurised ewes' milk without added starter culture, was examined by light microscopy and electron microscopy. Four populations of micro-organisms were recognized according to their position within the cheese: (i) those p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00375.x

    authors: Parker ML,Gunning PA,Macedo AC,Malcata FX,Brocklehurst TF

    更新日期:1998-04-01 00:00:00

  • The application of viable count procedures for measuring viable cells in the various marine environments.

    abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04411.x

    authors: Altug G,Cardak M,Ciftci PS,Gurun S

    更新日期:2010-01-01 00:00:00

  • Expression of the hilA Salmonella typhimurium gene in a poultry Salm. enteritidis isolate in response to lactate and nutrients.

    abstract::Pathogens express virulence genes in response to the combination of environmental conditions present in the host environment. The crop is the first gastrointestinal environment encountered in birds. However, feed withdrawal alters the crop environment resulting in an increased pH, and decreased concentrations of lacta...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01089.x

    authors: Durant JA,Corrier DE,Stanker LH,Ricke SC

    更新日期:2000-07-01 00:00:00

  • Viscosinamide, a new cyclic depsipeptide with surfactant and antifungal properties produced by Pseudomonas fluorescens DR54.

    abstract::Pseudomonas fluorescens DR54 showed antagonistic properties against plant pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. Antifungal activity was extractable from spent growth media, and fractionation by semi-preparative HPLC resulted in isolation of an active compound, which was identif...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00798.x

    authors: Nielsen TH,Christophersen C,Anthoni U,Sørensen J

    更新日期:1999-07-01 00:00:00

  • Variability and functional role of chromosomal sequences in 16SrI-B subgroup phytoplasmas including aster yellows and related strains.

    abstract:AIMS:Partial genetic characterization of several chromosomal regions on 35 16SrI-B phytoplasma strains maintained in periwinkle and collected in different geographical areas from plants of diverse species. METHODS AND RESULTS:Genes coding for ribosomal protein rpL22, elongation factor EF-Tu and random cloned sequences...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01809.x

    authors: Botti S,Bertaccini A

    更新日期:2003-01-01 00:00:00

  • The Pseudomonas group as an indicator of potential regrowth in water distribution systems.

    abstract::The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01021.x

    authors: Ribas F,Perramon J,Terradillos A,Frias J,Lucena F

    更新日期:2000-04-01 00:00:00

  • Antifungal activity of tyrosol and farnesol used in combination against Candida species in the planktonic state or forming biofilms.

    abstract:AIM:This study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. METHODS AND RESULTS:The effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentrati...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13513

    authors: Monteiro DR,Arias LS,Fernandes RA,Deszo da Silva LF,de Castilho MOVF,da Rosa TO,Vieira APM,Straioto FG,Barbosa DB,Delbem ACB

    更新日期:2017-08-01 00:00:00

  • Degradation of euptox A by tannase-producing rumen bacteria from migratory goats.

    abstract:AIMS:The gut microbiota capable of degrading plant biomass and antinutritional phytometabolites are of immense importance. This study reports isolation and characterization of tannase-producing rumen bacteria that could also degrade euptox A (9-oxo-10,11-dehydroageraphorone) present in Eupatorium adenophorum (Spreng). ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13563

    authors: Sharma D,Mal G,Kannan A,Bhar R,Sharma R,Singh B

    更新日期:2017-11-01 00:00:00

  • The survival of Salmonella enterica serovar Typhimurium DT104 and total viable counts on beef surfaces at different relative humidities and temperatures.

    abstract:AIMS:The aim of this study was to investigate changes in Salmonella and total viable count (TVC) survival on beef carcass surfaces stored for 72 h under different combinations of relative humidity (i.e. RH 75% or 96%) and temperature (5 degrees C or 10 degrees C). METHODS AND RESULTS:The influence of low water activit...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03989.x

    authors: Kinsella KJ,Prendergast DM,McCann MS,Blair IS,McDowell DA,Sheridan JJ

    更新日期:2009-01-01 00:00:00

  • Genetic fingerprinting of Flavobacterium columnare isolates from cultured fish.

    abstract:AIMS:To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. METHODS AND RESULTS:Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragm...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02314.x

    authors: Arias CR,Welker TL,Shoemaker CA,Abernathy JW,Klesius PH

    更新日期:2004-01-01 00:00:00

  • Polymorphism of Listeria monocytogenes and Listeria innocua strains isolated from short-ripened cheeses.

    abstract::Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00339.x

    authors: Margolles A,Mayo B,de los Reyes-Gavilán CG

    更新日期:1998-02-01 00:00:00

  • Adaptation of Pseudomonas aeruginosa ATCC 15442 to didecyldimethylammonium bromide induces changes in membrane fatty acid composition and in resistance of cells.

    abstract::The role of membrane fatty acid composition in the resistance of Pseudomonas aeruginosa ATCC 15442 to the bactericidal activity of didecyldimethyl ammonium bromide (DDAB) was investigated. In this study, the strain was sub-cultured in a medium with increasing DDAB concentrations. After adaptation, Ps. aeruginosa was a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00770.x

    authors: Méchin L,Dubois-Brissonnet F,Heyd B,Leveau JY

    更新日期:1999-05-01 00:00:00

  • Metabolic defence against oxidative stress: the road less travelled so far.

    abstract::Bacteria have survived, and many have thrived, since antiquity in the presence of the highly-reactive chalcogen-oxygen (O2 ). They are known to evoke intricate strategies to defend themselves from the reactive by-products of oxygen-reactive oxygen species (ROS). Many of these detoxifying mechanisms have been extensive...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13509

    authors: Lemire J,Alhasawi A,Appanna VP,Tharmalingam S,Appanna VD

    更新日期:2017-10-01 00:00:00

  • Rapid in vivo screening system for anti-oxidant activity using bacterial redox sensor strains.

    abstract:AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04514.x

    authors: Park SJ,Chung HY,Lee JH

    更新日期:2010-04-01 00:00:00

  • Impact of the addition of a nitrifying activated sludge on ammonia oxidation during composting of residual household wastes.

    abstract:AIMS:To investigate the nitrogen-microbial community dynamics during composting of a mixture of nitrifying waste activated sludge (WAS) and fine organic fraction of residual household waste (RHW). To examine whether the addition of nitrifying sludge could promote ammonia oxidation and reduce ammonia emissions. METHODS...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12651

    authors: Zeng Y,Dabert P,Le Roux S,Mognol J,De Macedo FJ,De Guardia A

    更新日期:2014-12-01 00:00:00

  • Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles.

    abstract::Although there is great scientific and technological interest in examining the physiology and bioenergetics of microorganisms from extreme environments, difficulties encountered in their cultivation and lack of genetic systems hampers the investigation of these issues. As such, we have adapted methods for continuous c...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.1998.tb05290.x

    authors: Rinker KD,Han CJ,Kelly RM

    更新日期:1998-12-01 00:00:00

  • Design and evaluation of PCR primers which differentiate Escherichia coli O157:H7 and related serotypes.

    abstract:AIMS:To develop methods to differentiate Escherichia coli O157:H7 and related serotypes by the use of amplicon length polymorphism (ALP) analysis based on identifying DNA sequence deletions within highly homologous regions of three sequenced E. coli strains. METHODS AND RESULTS:Potential primer locations along the anc...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03987.x

    authors: Rice WC

    更新日期:2009-01-01 00:00:00

  • Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease.

    abstract:AIMS:The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. METHODS AND RESULTS:Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01860.x

    authors: Larena I,De Cal A,Liñán M,Melgarejo P

    更新日期:2003-01-01 00:00:00

  • A novel echinocandin MIG0310 with anticandida activity from newly isolated Fusarium sp. strain MS-R1.

    abstract:AIM:To isolate and identify an anticandida compound from newly isolated Fusarium strain MS-R1 and characterization of its activity against standard and clinical strains of Candida. METHODS AND RESULTS:The fungal strain, Fusarium strain MS-R1, was isolated from soil. Molecular identification according to the internal t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12493

    authors: Masaphy S

    更新日期:2014-06-01 00:00:00

  • Construction of green fluorescent protein (GFP)-marked strains of Bradyrhizobium for ecological studies.

    abstract:AIM:To introduce the gfp gene encoding green fluorescent protein (GFP) into bradyrhizobia for their identification in nodules, soil and carrier-based inoculants. METHODS AND RESULTS:Bradyrhizobium sp. strains M29 and GN7, which nodulate mungbean (Vigna radiata), were conjugated with Escherichia coli S17-1 carrying pla...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01768.x

    authors: Bhatia R,Dogra RC,Sharma PK

    更新日期:2002-01-01 00:00:00

  • Microbiological characterization and probiotic potential of koko and koko sour water, African spontaneously fermented millet porridge and drink.

    abstract:AIMS:To identify and examine the diversity of predominant lactic acid bacteria (LAB) in koko and koko sour water (KSW) from different Ghanaian production sites with regard to pattern of fermentation (API 50 CHL), genotype, antimicrobial activity, and resistance to low pH and bile salts. METHODS AND RESULTS:In total 21...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2004.02162.x

    authors: Lei V,Jakobsen M

    更新日期:2004-01-01 00:00:00

  • Brettanomyces bruxellensis evolution and volatile phenols production in red wines during storage in bottles.

    abstract:AIMS:The presence of Brettanomyces bruxellensis is an important issue during winemaking because of its volatile phenols production capacities. The aim of this study is to provide information on the ability of residual B. bruxellensis populations to multiply and spoil finished wines during storage in bottles. METHODS A...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04561.x

    authors: Coulon J,Perello MC,Lonvaud-Funel A,de Revel G,Renouf V

    更新日期:2010-04-01 00:00:00

  • Thyme essential oil as an alternative mechanism: biofungicide-causing sensitivity of Mycosphaerella graminicola.

    abstract:AIMS:To understand the mode of action of thyme essential oil as an alternative biofungicide. METHODS AND RESULTS:The chemical composition of thyme essential oil isolated by hydrodistillation from the aerial parts of Thymus vulgaris was analyzed. The main constituents of thyme essential oil were thymol (76·96%), ρ-cyme...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13408

    authors: Ben Jabeur M,Somai-Jemmali L,Hamada W

    更新日期:2017-04-01 00:00:00

  • Proteomic identification of membrane proteins regulating antimicrobial peptide resistance in Vibrio parahaemolyticus.

    abstract:AIMS:To identify proteins regulating antimicrobial peptide (AMP) resistance in Vibrio parahaemolyticus using membrane subproteome analysis. METHODS AND RESULTS:Three synthetic AMPs (Q4, Q6 and H1) and a natural one from fish (pleurocidin) were used for selection of AMP-resistant strains. Differential expression patter...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04544.x

    authors: Shen CJ,Kuo TY,Lin CC,Chow LP,Chen WJ

    更新日期:2010-04-01 00:00:00

  • Relationships between the density of different indicator organisms on sheep and beef carcasses and in frozen beef and sheep meat.

    abstract:AIM:To describe the relationship between the concentration of different indicator bacteria in red meat. METHODS AND RESULTS:Enumeration data for aerobic plate count (APC), Enterobacteriaceae, coliforms and Escherichia coli biotype I were analysed from an Australia-wide survey of beef carcasses, sheep carcasses, frozen...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03060.x

    authors: Jordan D,Phillips D,Sumner J,Morris S,Jenson I

    更新日期:2007-01-01 00:00:00

  • Evidence of gushing induction by Penicillium oxalicum proteins.

    abstract:AIMS:The aim of this study was to elucidate whether grape-associated fungi exert an influence on gushing by their production of surface-active compounds. METHODS AND RESULTS:In preliminary experiments, 58 grape-associated isolates of species within Penicillium and Aspergillus genera were tested for their ability to mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13366

    authors: Vogt EI,Kupfer VM,Vogel RF,Niessen L

    更新日期:2017-03-01 00:00:00

  • Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China.

    abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12456

    authors: Li D,Gu AZ,He M

    更新日期:2014-06-01 00:00:00

  • Low light intensity and nitrogen starvation modulate the chlorophyll content of Scenedesmus dimorphus.

    abstract:AIMS:Chlorophyll is a photosynthetic pigment found in plants and algal organisms and is a bioproduct with human health benefits and a great potential for use in the food industry. The chlorophyll content in microalgae strains varies in response to environmental factors. In this work, we assessed the effect of nitrogen ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13007

    authors: Ferreira VS,Pinto RF,Sant'Anna C

    更新日期:2016-03-01 00:00:00

  • Validation of the dorsal air pouch model to predict and examine immunostimulatory responses in the gut.

    abstract:AIMS:To validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics Lactobacillus paracasei subsp. paracasei B112, DC205, DC215 and DC412 strains in the gut mucosa. METHODS AND RESULTS:Only the DC412 strain interacted strongly with the cells forming the ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04421.x

    authors: Kourelis A,Zinonos I,Kakagianni M,Christidou A,Christoglou N,Yiannaki E,Testa T,Kotzamanidis C,Litopoulou-Tzanetaki E,Tzanetakis N,Yiangou M

    更新日期:2010-01-01 00:00:00

  • Growth, pigment production and protease activity of Monascus purpureus as affected by salt, sodium nitrite, polyphosphate and various sugars.

    abstract::The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00821.x

    authors: Tseng YY,Chen MT,Lin CF

    更新日期:2000-01-01 00:00:00