Abstract：
AIMS:To understand the mode of action of thyme essential oil as an alternative biofungicide. METHODS AND RESULTS:The chemical composition of thyme essential oil isolated by hydrodistillation from the aerial parts of Thymus vulgaris was analyzed. The main constituents of thyme essential oil were thymol (76·96%), ρ-cymene (9·89%), γ-terpinene (1·92%) and caryophyllene oxide (1·69%). The antifungal activity of the oil and its pure major component (thymol) was assessed by the in vitro assay against Mycosphaerella graminicola. Thyme oil exhibited higher antifungal activity than thymol. The expression pattern of genes involved in fungal development and detoxification acting in M. graminicola under thyme oil and thymol treatment was analyzed. Thyme oil overexpressed, more than thymol, the genes encoding for the efflux pump (MgMfs1, MgAtr4), the regulatory subunit of protein kinase A (PKA) (MgBcy1) and the MAPK MgHog1. Thyme oil repressed the expression of the genes encoding for the efflux pump MgAtr4, the MAPK (MgSlt2) and the regulatory subunit of PKA (MgBcy1). However, thymol repressed only MgAtr4 and MgSlt2 expression. CONCLUSIONS:These data highlight the ability of thyme oil to target genes involved in fungal development and virulence of the yeast-like fungi M. graminicola, which explain its higher antifungal activity. SIGNIFICANCE AND IMPACT OF THE STUDY:These findings will probably be useful to design an alternative biofungicide which will not lead to pathogen multidrug resistance.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Ben Jabeur M,Somai-Jemmali L,Hamada Wdoi
10.1111/jam.13408subject
Has Abstractpub_date
2017-04-01 00:00:00pages
932-939issue
4eissn
1364-5072issn
1365-2672journal_volume
122pub_type
杂志文章abstract：AIMS:To understand factors that impact solar-powered electricity generation by Rhodobacter sphaeroides in a single-chamber microbial fuel cell (MFC). METHODS AND RESULTS:The MFC used submerged platinum-coated carbon paper anodes and cathodes of the same material, in contact with atmospheric oxygen. Power was measured ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2007.03580.x
更新日期：2008-03-01 00:00:00
abstract：AIMS:Dielectric barrier discharge (DBD) plasma is used for sterilization of contaminated inanimate surfaces but seldomly optimized and depends upon the type of organisms and the plasma treatment duration, (net energy deposited) this efficacy varies. The proposed study was designed to see biological responses of one of ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2010.04834.x
更新日期：2010-12-01 00:00:00
abstract：AIMS:To analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. METHODS AND RESULTS:By using random amplified polymorphic DNA (RAPD) P...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12858
更新日期：2015-09-01 00:00:00
abstract：AIMS:The aim was to evaluate (i) the resistance of Escherichia coli BJ4 to citral in a buffer system as a function of citral concentration, treatment medium pH, storage time and initial inoculum size, (ii) the role of the sigma factor RpoS on citral resistance of E. coli, (iii) the role of the cell envelope damage in t...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04597.x
更新日期：2010-06-01 00:00:00
abstract：AIMS:The objective of this study was to obtain a phenotypic and genotypic profile of Salmonella enterica including multidrug-resistant (MDR) isolates from food-producing animals and clinical isolates, as well as their genetic relatedness in two different States of Mexico (Jalisco and State of Mexico). METHODS AND RESU...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13615
更新日期：2018-01-01 00:00:00
abstract：AIMS:To evaluate four DNA extraction methods to elucidate the most effective method for bacterial DNA recovery from human milk (HM). METHODS AND RESULTS:Human milk DNA was extracted using the following methods: (i) Qiagen MagAttract Microbial DNA Isolation Kit (kit QM), (ii) Norgen Milk Bacterial DNA Isolation Kit (ki...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14780
更新日期：2021-01-01 00:00:00
abstract：AIMS:The aim of this study was to characterize anaerobic ammonium oxidation (anammox) community in sediments of the Dongjiang River, a drinking water source of Hong Kong. METHODS AND RESULTS:The diversity and distribution of the anammox community were investigated based on a comparative analyses of 16S rRNA and hydraz...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12367
更新日期：2014-02-01 00:00:00
abstract：:The exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in Lysobacter, 11 new species having been described since 2005, and in Stenotrophomonas with six new species since 2000. The new species of Lysobacter, isol...
journal_title：Journal of applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-2672.2009.04471.x
更新日期：2010-03-01 00:00:00
abstract：:Two abundant surface proteins, EA1 and Sap, are components of the Bacillus anthracis surface layer (S-layer). Their corresponding genes have been cloned, shown to be clustered on the chromosome and sequenced. EA1 and Sap each possess three 'S-layer homology' motifs. Single and double disrupted mutants were constructed...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1999.00882.x
更新日期：1999-08-01 00:00:00
abstract：:Levels of somatic and F-specific coliphages, and phages infecting Bacteroides fragilis were measured in 257 samples collected in different freshwater environments with different levels and characteristics of faecal pollution. In samples with recent pollution of domestic origin, the numbers of the three groups of phage...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1997.00354.x
更新日期：1997-03-01 00:00:00
abstract：:Thirty isolates of Listeria monocytogenes and 18 of L. innocua obtained from different short-ripened cheeses manufactured in Asturias (northern Spain), were compared with each other and with reference strains using serotype, phage type and pulsed-field restriction endonuclease digestion profiles analysis of the total ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1998.00339.x
更新日期：1998-02-01 00:00:00
abstract：AIMS:To evaluate the production and stability of laccases by Pleurotus ostreatus in liquid co-cultures with Trichoderma viride as a function of infection time and agitation rate. METHODS AND RESULTS:Pleurotus ostreatus cultures were infected with T. viride spores at 30 and 48 h. Maximal laccase volumetric activity was...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04493.x
更新日期：2010-03-01 00:00:00
abstract：AIMS:To calculate fermentation efficiency in a continuous ethanol production process, we aimed to develop a robust mathematical method based on the analysis of metabolic by-product formation. METHODS AND RESULTS:This method is in contrast to the traditional way of calculating ethanol fermentation efficiency, where the...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13240
更新日期：2016-10-01 00:00:00
abstract：AIMS:To validate an in vitro model for the analysis of physiological and ecological responses to sugar challenge in bacterial populations, and subsequent changes in enamel mineralization. METHODS AND RESULTS:A seven-organism bacterial consortium was grown in a biofilm mode on enamel and hydroxyapatite (HA) surfaces in...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2001.01263.x
更新日期：2001-03-01 00:00:00
abstract：AIMS:To obtain bacteria with PKS (polyketide synthase) genes and antimicrobial activity from sponges. METHODS AND RESULTS:Eighteen bacteria with KS (ketosynthase) genes were identified by polymerase chain reaction (PCR) screening of 98 isolates from South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysid...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04241.x
更新日期：2009-08-01 00:00:00
abstract：AIMS:To evaluate the performance of three Salmonella plating media (Rambach, Xylose Lysine Deoxycholate agar and modified Brilliant Green Agar plus Novobiocin) as part of the ISO 6579: 2002 (Annex D) on poultry environmental samples. METHODS AND RESULTS:The samples analysed were those for the European Union Salmonella...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04386.x
更新日期：2009-12-01 00:00:00
abstract：AIMS:We aimed at expressing heterologous proteins in Paenibacillus larvae, the causative agent of American Foulbrood of honey bees, as a prerequisite for future studies on the molecular pathogenesis of P. larvae infections. METHODS AND RESULTS:For this purpose, we established a protocol for the transformation of the p...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2011.05214.x
更新日期：2012-03-01 00:00:00
abstract：AIMS:The aims of this study were to characterize the antibacterial activity and the chemotype of Litsea cubeba leaf essential oil (EO) harvested in North Vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (Cyprinus carpio) challenged ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13160
更新日期：2016-08-01 00:00:00
abstract：AIMS:The objective of this study was to compare qualitatively and quantitatively the results of identification of the bacteria present in milk samples from cows with subclinical mastitis using multiplex qPCR assay and matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS® ) after ba...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14358
更新日期：2019-09-01 00:00:00
abstract：AIMS:Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but nonculturable (VBNC) micro-organisms and nonviable cells. The aim of the study was to evaluate flow cytometry as a novel me...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13666
更新日期：2018-02-01 00:00:00
abstract：AIM:Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch-blood-egg yolk-polymyxin B-trimethoprim-ceftazidime (SBYPTC) agar was established to isolate and specify the number of Bacillus cereus in food products. METHODS AND RESULTS:The effectiveness of ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.14561
更新日期：2020-05-01 00:00:00
abstract：AIMS:To investigate multiple tolerance of Saccharomyces cerevisiae obtained through a laboratory strategy of adaptive evolution in acetic acid, its relation with enzymatic ROS detoxification and bioethanol 2G production. METHODS AND RESULTS:After adaptive evolution in acetic acid, a clone (Y8A) was selected for its to...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.13917
更新日期：2018-09-01 00:00:00
abstract：:Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes fr...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2009.04578.x
更新日期：2010-05-01 00:00:00
abstract：AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02242.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To determine the reliable combination of protocols for specific detection and identification of R. solanacearum race 3 biovar 2 (R3bv2) through a comprehensive comparison among currently available techniques. METHODS AND RESULTS:Sensitivity and specificity of the conventional isolation, bioassay, serological assa...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12585
更新日期：2014-10-01 00:00:00
abstract：:The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.2000.00821.x
更新日期：2000-01-01 00:00:00
abstract：AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2004.02289.x
更新日期：2004-01-01 00:00:00
abstract：AIMS:To evaluate the potential for developing a quality index for a Danish modified atmosphere packaged (MAP) heat-processed and naturally contaminated pork meat product stored at 5 degrees C. METHODS AND RESULTS:The composition of the predominating microflora and changes in contents of tyramine, arginine, organic aci...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1365-2672.2008.04045.x
更新日期：2009-02-01 00:00:00
abstract：AIMS:The environmental pathogen Streptococcus uberis causes intramammary infections in dairy cows. Because biofilm growth might contribute to Strep. uberis mastitis, we conducted a biological screen to identify genes potentially involved in the regulation of biofilm growth. METHODS AND RESULTS:By screening a transposo...
journal_title：Journal of applied microbiology
pub_type： 杂志文章
doi：10.1111/jam.12664
更新日期：2015-01-01 00:00:00
