Abstract:
AIMS:To evaluate the potential for developing a quality index for a Danish modified atmosphere packaged (MAP) heat-processed and naturally contaminated pork meat product stored at 5 degrees C. METHODS AND RESULTS:The composition of the predominating microflora and changes in contents of tyramine, arginine, organic acids and sensory characteristics were analysed. The microflora was predominated by Lactobacillus sakei, Leuconostoc carnosum and Carnobacterium divergens. The presence of each species varied between products and batches resulting in limited usefulness of the concentrations of these bacteria or their metabolites as indices of quality. Furthermore, the three species differed in their metabolic activities as shown by use of a model meat extract. However, when MAP storage of the processed pork product was followed by aerobic storage then acetic acid showed some potential as a chemical indicator of sensory quality. CONCLUSION:Variation in processing parameters and spoilage microbiota limited the usefulness of concentrations of micro-organisms and their metabolites as indices of spoilage for the studied processed MAP pork product. SIGNIFICANCE AND IMPACT OF THE STUDY:The present study contributes to an understanding of the difficulties experienced in developing quality indices to be used in the control of microbial spoilage of processed MAP meat products.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Laursen BG,Byrne DV,Kirkegaard JB,Leisner JJdoi
10.1111/j.1365-2672.2008.04045.xsubject
Has Abstractpub_date
2009-02-01 00:00:00pages
543-53issue
2eissn
1364-5072issn
1365-2672pii
JAM4045journal_volume
106pub_type
杂志文章abstract:AIM:The effect of heavy metals presence on the decolourization of Reactive Black 5 by Pseudomonas aeruginosa was evaluated. METHODS AND RESULTS:In the current study, a newly isolated strain identified as P. aeruginosa strain Gb 30 was selected for its ability to remove high concentration of Reactive Black 5 and resist...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14262
更新日期:2019-06-01 00:00:00
abstract:AIM:To analyse the physiological response of Sphingobium sp. 22B to water stress. METHODS AND RESULTS:The strain was grown under excess of carbon source and then subjected to low (60RH) and high (18RH) water stress conditions for 96 h. Quantification of trehalose, glycogen, polyhydroxybutyrate (PHB) and transmission e...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13742
更新日期:2018-06-01 00:00:00
abstract:AIMS:To develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsrA) for use in quantitative real-time PCR detection of sulfate-reducing bacteria (SRB) in stored swine manure. METHODS AND RESULTS:Degenerate primer sets were developed to detect SRB in stored swine manure. These were comp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03900.x
更新日期:2008-12-01 00:00:00
abstract:AIM:The study was carried out to assess the agreement of API 50 CH fermentation data of food lactobacilli with their RAPD profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. METHODS AND RESULTS:API 50 CH, RAPD and DNA:DNA reassociation data for 42 lactobacilli from...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01202.x
更新日期:2000-12-01 00:00:00
abstract:AIMS:The aim of the present study was to develop a colorimetric LAMP assay for the detection of enteroviruses belonging to species A-D targeting the 5' untranslated region (5' UTR) of enteroviruses genome. METHODS AND RESULTS:The RNA was converted to cDNA by the reverse transcriptase and then amplified via LAMP by the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14770
更新日期:2021-01-01 00:00:00
abstract::Probiotics are live micro-organisms with beneficial effects on human health, which have the ability to counteract infections at different locations of the body. Clinical trials have shown that probiotics can be used as preventive and therapeutic agents in upper respiratory tract infections (URTIs) and otitis. Their me...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2012.05394.x
更新日期:2012-12-01 00:00:00
abstract:AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02700.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:Intraspecific differentiation and characterization of Vibrio alginolyticus strains isolated from cultured fish in Spain. MATERIALS AND RESULTS:Thirty-four Vibrio alginolyticus strains isolated from cultured fish were intraspecifically characterized on the basis of biochemical and exoenzymatic patterns, outer memb...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02078.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Utilization of silage in livestock farming is expected to increase in developing countries in the tropical and subtropical parts of the world. The aim of this study was to investigate the influence of nitrogen fertilization on the chemical composition of herbage, ensiling process and silage quality, and to contrib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04591.x
更新日期:2010-06-01 00:00:00
abstract:AIMS:To identify the chemical structure of a bacteriocin, thermophilin 1277, produced by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277 was purified and partial N-terminal sequence analysis revealed 6 unidentified amino acids amongst 31 amino acids residues. A 2.7-kbp region containing the t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04059.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:To develop and evaluate a novel genotypic test for rapid detection of rifampicin and isoniazid resistance of multidrug-resistant (MDR) Mycobacterium tuberculosis isolates by a multiplex probe array. METHODS AND RESULTS:A multiplex probe array was designed for genotypic test to simultaneously screen the mutations ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03350.x
更新日期:2007-10-01 00:00:00
abstract:AIMS:To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS:Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in bi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04508.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01727.x
更新日期:2002-01-01 00:00:00
abstract::The lethal toxin (LeTx) of Bacillus anthracis is the major virulence factor responsible for the death of infected animals and for cytolysis of cultured macrophages. Its catalytic component, LF, contains the characteristic zinc-binding motif of metalloproteases, it binds zinc and indirect evidence suggests that this hy...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00893.x
更新日期:1999-08-01 00:00:00
abstract:AIMS:The aims of the study were to characterize the O91 O-antigen gene cluster from Shiga toxin-producing Escherichia coli (STEC) O91 and to provide the basis for a specific PCR test for rapid detection of E. coli O91. METHODS AND RESULTS:The published primers complementary to JUMPstart and gnd gene, the conserved fla...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01743.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05277.x
更新日期:2012-05-01 00:00:00
abstract:AIMS:To assess the behaviour of Aspergillus section Nigri IOC 4573 in mango nectar as affected by temperature and pH. METHODS AND RESULTS:A central composite design (2(2)+2*2+3) was used to estimate the influence of temperature (17·2-22·8°C) and pH (3·28-4·7) on A. section Nigri growth (λ, lag time; μ, growth rate). S...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04803.x
更新日期:2010-09-01 00:00:00
abstract:AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02571.x
更新日期:2005-01-01 00:00:00
abstract::A bacterium (strain TJ330) capable of using carbon disulphide (CS2) as its sole energy source in an acidic environment was isolated from a peat biofilter used in experiments to remove CS2 and hydrogen sulphide (H2S) from air. Its physiology and taxonomy are described here. The strain oxidized CS2, H2S and elemental su...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01150.x
更新日期:2000-10-01 00:00:00
abstract:AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01200.x
更新日期:2001-01-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02242.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Non-Tuberculous Mycobacteria (NTM) are ubiquitous in nature. The data on prevalence of NTM under the RNTCP is scarce. Many NTM species have clinical significance, and hence their identification and speciation are important. METHODS AND RESULTS:It is a cross-sectional study conducted at the five RNTCP accredited c...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13604
更新日期:2018-01-01 00:00:00
abstract:AIMS:The purpose of this study was to purify and characterize a bacteriocin produced by Eikenella corrodens A32E2. METHODS AND RESULTS:Peptostreptococcus anaerobius ATCC27337 was used as indicator strain in antagonistic assays for bacteriocin-producing E. corrodens A32E2. Protein extraction was influenced by pH and bu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03565.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:The biopesticide effect of four green composts against fusarium wilt in melon plants and the effect of soil quality in soils amended with composts were assayed. METHODS AND RESULTS:The composts consisted of pruning wastes, with or without addition of coffee wastes (3/1 and 4/1, dry wt/dry wt) or urea (1000/1, dry...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02508.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine the composition and temporal stability of the gut (faecal) microbiota of sheep (Ovis aries). METHODS AND RESULTS:Microbial population dynamics was conducted using ARISA (28 sheep) and 16S rRNA sequencing (11 sheep). Firmicutes and Bacteroidetes were the predominant bacterial phyla, constituting ~80% ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14468
更新日期:2020-01-01 00:00:00
abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04411.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12767
更新日期:2015-05-01 00:00:00
abstract:AIMS:To investigate the influence of aerobic or vacuum pack storage of beef trimmings on the microbiology, colour and odour of subsequently produced mince. METHODS AND RESULTS:Trimmings stored aerobically for 7 or 10 days and in vacuum packs for 7, 10, 14 or 22 days at 0 or 5°C were minced, stored aerobically at 0 or ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04755.x
更新日期:2010-10-01 00:00:00
abstract:AIMS:Optimization of traditional processing of soybeans using response surface methodology (RSM) to achieve a minimum level of antinutritional factors (ANFs) in kinema. METHODS AND RESULTS:Central composite rotatable designs were used to optimize the processing stages of kinema preparation. In each stage, the linear o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12826
更新日期:2015-07-01 00:00:00
abstract::During the first half of the century, the majority of anthrax outbreaks in Canada occurred in the southern portions of Ontario and Quebec and were often associated with pastures contaminated by effluent from textile industries dealing with imported animal materials. In 1952, introduction of Federal regulations requiri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2672.1999.00872.x
更新日期:1999-08-01 00:00:00