Abstract:
AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by sodium phytate. Inclusion of glucose into rice bran-soybean milk (RB-SM) medium inhibited the phytase production. Supplementation of 0.05-0.5% of phosphate to RB-SM medium neither inhibited nor stimulated the phytase production. The optimum pH and temperature for the phytase production of M. jalaludinii were 7.02 and 39 degrees C, respectively. Surfactants such as Tween-80 (0.5% and 1.0%) and Triton X-100 (1.0%) did not affect the phytase production. CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY:The study has provided important data on the culture conditions affecting phytase production of M. jalaludinii, which will facilitate future research on the optimization of fermentation processes for production of high phytase activity by M. jalaludinii.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Lan GQ,Abdullah N,Jalaludin S,Ho YWdoi
10.1046/j.1365-2672.2002.01727.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
668-74issue
4eissn
1364-5072issn
1365-2672pii
1727journal_volume
93pub_type
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