Development and comparison of SYBR Green quantitative real-time PCR assays for detection and enumeration of sulfate-reducing bacteria in stored swine manure.

abstract：AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...

journal_title：Journal of applied microbiology

authors： Boehm AB,Griffith J,McGee C,Edge TA,Solo-Gabriele HM,Whitman R,Cao Y,Getrich M,Jay JA,Ferguson D,Goodwin KD,Lee CM,Madison M,Weisberg SB

更新日期：2009-11-01 00:00:00

abstract：AIMS:Four commercially available robotic vacuum cleaners were assessed for sampling efficiency of wet disseminated Bacillus atrophaeus spores on carpet, polyvinyl chloride (PVC) and laminate flooring. Furthermore, their operability was evaluated and decontamination efficiency of one robot was assessed, using a sodium h...

journal_title：Journal of applied microbiology

authors： Thompson KA,Paton S,Pottage T,Bennett AM

更新日期：2018-10-01 00:00:00

abstract：AIM:To determine the effect of different carbohydrate-based finishing diets on fermentation characteristics and the shedding of Escherichia coli and enterohaemorrhagic E. coli (EHEC) virulence genes in cattle faeces. METHODS AND RESULTS:The size of faecal E. coli populations and fermentation characteristics were ascer...

journal_title：Journal of applied microbiology

authors： Gilbert RA,Tomkins N,Padmanabha J,Gough JM,Krause DO,McSweeney CS

更新日期：2005-01-01 00:00:00

abstract：AIMS:This work aimed at using a pool of selected enterococci and fungal proteases to hydrolyse wheat gluten during long-time fermentation. METHODS AND RESULTS:A liquid dough made with wheat flour (20% w/w) was fermented with three Enterococcus strains (dough A) or with the combination of enterococci and Rhizopus oryza...

journal_title：Journal of applied microbiology

authors： M'hir S,Rizzello CG,Di Cagno R,Cassone A,Hamdi M

更新日期：2009-02-01 00:00:00

abstract：AIMS:Salmonella Hadar, Salmonella Brancaster and Salmonella Enteritidis are the main Salmonella enterica ssp. enterica serovars isolated from poultry in Senegal. Our objective was to analyse the pulsed-field gel electrophoresis (PFGE) and antibioresistance patterns of strains belonging to these serovars and to assess t...

journal_title：Journal of applied microbiology

authors： Cardinale E,Perrier Gros-Claude JD,Rivoal K,Rose V,Tall F,Mead GC,Salvat G

更新日期：2005-01-01 00:00:00

abstract：:The distribution of micro-organisms in mature Serra, a traditional Portuguese cheese made from unpasteurised ewes' milk without added starter culture, was examined by light microscopy and electron microscopy. Four populations of micro-organisms were recognized according to their position within the cheese: (i) those p...

journal_title：Journal of applied microbiology

authors： Parker ML,Gunning PA,Macedo AC,Malcata FX,Brocklehurst TF

更新日期：1998-04-01 00:00:00

abstract：AIMS:The objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against Listeria monocytogenes in a model meat marinade and on marinated pork tenderloins. METHODS AND RESULTS:In a first s...

journal_title：Journal of applied microbiology

authors： Kramer B,Thielmann J,Hickisch A,Muranyi P,Wunderlich J,Hauser C

更新日期：2015-03-01 00:00:00

abstract：AIMS:Algae are favourable as a biofuel source because of the potential high oil content and fast generation of biomass. However, one of the challenges for this technology is achieving high oil content while maintaining exponential or high growth of the organism. Introducing a two-stage reactor to optimize both growth a...

journal_title：Journal of applied microbiology

authors： Csavina JL,Stuart BJ,Riefler RG,Vis ML

更新日期：2011-08-01 00:00:00

abstract：AIMS:The aim of this study is to report on the hydrolytic action of Alcaligenes faecalis isolated from soil samples and its ability to degrade ochratoxin A. METHODS AND RESULTS:An A. faecalis strain was identified and characterized by employing both a phenotypic analysis and 16S rDNA sequence analysis. The results sho...

journal_title：Journal of applied microbiology

authors： Zhang HH,Wang Y,Zhao C,Wang J,Zhang XL

更新日期：2017-09-01 00:00:00

abstract：AIMS:Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the ...

journal_title：Journal of applied microbiology

authors： Zwe YH,Yuk HG

更新日期：2020-12-01 00:00:00

abstract：:The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...

journal_title：Journal of applied microbiology

authors： Wang Y,McAllister TA,Yanke LJ,Cheeke PR

更新日期：2000-05-01 00:00:00

abstract：AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...

journal_title：Journal of applied microbiology

authors： Bravo SME,Morales M,Del Mónaco SM,Caballero AC

更新日期：2019-05-01 00:00:00

abstract：AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...

journal_title：Journal of applied microbiology

authors： Guo XL,Leng P,Yang Y,Yu LG,Lou HX

更新日期：2008-03-01 00:00:00

abstract：:Medical devices provide critical care and diagnostic applications through patient contact. Sterility assurance level (SAL) may be defined as the probability of a single viable micro-organism occurring on an item after a sterilization process. Sterilization microbiology often relies upon using an overkill validation me...

journal_title：Journal of applied microbiology

authors： McEvoy B,Lynch M,Rowan NJ

更新日期：2020-11-06 00:00:00

abstract：AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...

journal_title：Journal of applied microbiology

authors： Brandl H,Bachofen R,Bischoff M

更新日期：2005-01-01 00:00:00

abstract：AIMS:To investigate the biofilm inhibitory activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against Staphylococcus aureus and Pseudomonas aeruginosa biofilms. METHODS AND RESULTS:The pulp of red pitahaya and the leaves of red spinach were extracted using methanol fo...

journal_title：Journal of applied microbiology

authors： Yong YY,Dykes G,Lee SM,Choo WS

更新日期：2019-01-01 00:00:00

abstract：AIM:This study focuses on investigating the molecular and physiological characteristics of Prevotella intermedia after molecular oxygen exposure (MOE) and the effect on drug susceptibility patterns. METHODS AND RESULTS:Samples of P. intermedia were used as parent strains: ATCC25611 and four clinical isolates. Strains ...

journal_title：Journal of applied microbiology

authors： dos Santos SG,Diniz CG,da Silva VL,Souza NC,de Lima FL,Bomfim MR,de Carvalho MA,Farias LM

更新日期：2007-10-01 00:00:00

abstract：:Cancer is a prominent cause of morbidity and mortality worldwide, in spite of advances in therapeutic interventions and supportive care. In 2018 alone, there were 18·1 million new cancer cases and 9·6 million deaths indicating the need for novel anticancer agents. Plant-based products have often been linked with prote...

journal_title：Journal of applied microbiology

authors： Soopramanien M,Khan NA,Siddiqui R

更新日期：2020-12-23 00:00:00

abstract：AIMS:In biosand filters (BSF), treatment is largely driven by the development of a biolayer (schmutzdecke) which establishes itself during the startup phase. In this study, the effect of changing influent total organic carbon (TOC) loading on the removal efficiency of Vibrio cholerae in laboratory-operated BSFs was qua...

journal_title：Journal of applied microbiology

authors： Danley-Thomson AA,Huang EC,Worley-Morse T,Gunsch CK

更新日期：2018-09-01 00:00:00

abstract：AIMS:To compare the production of recombinant cellulase enzymes in two Saccharomyces species so as to ascertain the most suitable heterologous host for the degradation of cellulose-based biomass and its conversion into bioethanol. METHOD AND RESULTS:cDNA copies of genes representing the three major classes of cellulas...

journal_title：Journal of applied microbiology

authors： Fitzpatrick J,Kricka W,James TC,Bond U

更新日期：2014-07-01 00:00:00

abstract：AIMS:To develop descriptive models for the combined effect of temperature (10-40 degrees C) and water activity (0.850-0.980) on the growth of two ochratoxin A producing strains of Aspergillus carbonarius from Greek wine grapes on a synthetic grape juice medium. METHODS AND RESULTS:Fungal growth was measured as changes...

journal_title：Journal of applied microbiology

authors： Tassou CC,Panagou EZ,Natskoulis P,Magan N

更新日期：2007-12-01 00:00:00

abstract：AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...

journal_title：Journal of applied microbiology

authors： Guo L,Long M,Huang Y,Wu G,Deng W,Yang X,Li B,Meng Y,Cheng L,Fan L,Zhang H,Zou L

更新日期：2015-07-01 00:00:00

abstract：AIMS:To isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (SPs) in sheep dips. METHODS AND RESULTS:SP-degrading bacteria were isolated from a mixed soil sample consisting of garden soil and soils from farms where SPs had been used. The two largest in size were then iden...

journal_title：Journal of applied microbiology

authors： Grant RJ,Daniell TJ,Betts WB

更新日期：2002-01-01 00:00:00

abstract：:Due to the increasing number of nosocomial infections and multidrug-resistant bacterial strains, Staphylococcus aureus is now a major worldwide concern. Rapid detection and characterization of this bacterium has become an important issue for biomedical applications. Biosensors are increasingly appearing as low-cost, e...

journal_title：Journal of applied microbiology

authors： Templier V,Roupioz Y

更新日期：2017-11-01 00:00:00

abstract：:The inactivation of Bacillus subtilis spores by ultrasonic treatments under static pressure (Mano-Sonication, MS) and a combined MS/heat treatment (Mano-Thermo-Sonication) was investigated. The sporicidal effect of MS treatments depended on static pressure, amplitude of ultrasonic waves and treatment temperature. At 7...

journal_title：Journal of applied microbiology

authors： Raso J,Palop A,Pagán R,Condón S

更新日期：1998-11-01 00:00:00

abstract：AIMS:To investigate the effects of the endophyte Bacillus subtilisALB629 on the growth of cacao seedlings at early developmental stage and to evaluate its antimicrobial properties. METHODS AND RESULTS:Germinating cacao seeds were inoculated with ALB629, and seedlings growth was evaluated 30 days later. Significant inc...

journal_title：Journal of applied microbiology

authors： Falcäo LL,Silva-Werneck JO,Vilarinho BR,da Silva JP,Pomella AW,Marcellino LH

更新日期：2014-06-01 00:00:00

abstract：AIMS:The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and...

journal_title：Journal of applied microbiology

authors： Novinscak A,Filion M

更新日期：2019-07-01 00:00:00

abstract：AIMS: To develop a novel Vero cell assay that implements a real-time cell electronic sensing (RT-CES) system for the determination of the presence of verotoxin-producing Escherichia coli (VTEC). The assay overcomes the major drawbacks in conventional Vero cell assay, for example, labour-intensive and time-consuming. M...

journal_title：Journal of applied microbiology

authors： Xing JZ,Zhu L,Huang B,Chen J,Gabos S

更新日期：2012-08-01 00:00:00

abstract：AIM:Nano-biotechnologically synthesizing silver nanoparticles via canthaxanthin pigment extracted from Dietzia maris AURCCBT01 and assessing their cytotoxic therapeutic potential against human keratinocyte cell line (HaCaT) were the key objectives of this study. METHODS AND RESULTS:The pigment extracted from D. maris ...

journal_title：Journal of applied microbiology

authors： Venil CK,Malathi M,Velmurugan P,Renuka Devi P

更新日期：2020-10-08 00:00:00

abstract：AIMS:To study the phenotypic characteristics of Aeromonas spp. from environmental and clinical samples in Spain and to cluster these strains by numerical taxonomy. METHODS AND RESULTS:A collection of 202 Aeromonas strains isolated from bivalve molluscs, water and clinical samples was tested for 64 phenotypic propertie...

journal_title：Journal of applied microbiology

authors： Miñana-Galbis D,Farfán M,Lorén JG,Fusté MC

更新日期：2002-01-01 00:00:00