Abstract:
AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory concentrations (MICs) of disinfectants were determined using the agar dilution method. All isolates were screened for the presence of antimicrobial and disinfectant resistance genes and further analysed for genetic relatedness by pulse-field gel electrophoresis (PFGE). Results showed that 46·6% of the isolates were resistant to at least one antimicrobial. Escherichia coli isolates showed resistance to fewer antimicrobials as panda age increased. Among antimicrobial-resistant E. coli isolates, the antimicrobial resistance genes blaCTX-M (88·2%) and sul1 (92·3%) were most prevalent. The disinfectant resistance genes emrE, ydgE/ydgF, mdfA and sugE(c) were commonly present (68·2-98·9%), whereas qac and sugE(p) were relatively less prevalent (0-21·3%). The frequencies of resistance genes tended to be higher in E. coli isolated in December than in July, and PFGE profiles were also more diverse in isolates in December. The qacEΔ1 and sugE(p) genes were higher in adolescent pandas than in any other age groups. PFGE revealed that antimicrobial resistance correlated well with sampling time and habitat. CONCLUSIONS:This study demonstrated that antimicrobial and disinfectant resistance was common in giant panda-derived E. coli, and the antimicrobial resistance was associated with sampling time and habitat. Escherichia coli could serve as a critical vector in spreading disinfectant and antimicrobial resistance. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first study that demonstrated the phenotypic and genetic characterizations of antimicrobial and disinfectant resistance in E. coli isolates from more than 60 giant pandas. Frequent transfer of pandas to other cages may lead to the dissemination of antimicrobial resistance. The study highlights the need for regularly monitoring the antimicrobial and disinfectant resistance in bacteria from giant pandas.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Guo L,Long M,Huang Y,Wu G,Deng W,Yang X,Li B,Meng Y,Cheng L,Fan L,Zhang H,Zou Ldoi
10.1111/jam.12820subject
Has Abstractpub_date
2015-07-01 00:00:00pages
55-64issue
1eissn
1364-5072issn
1365-2672journal_volume
119pub_type
杂志文章abstract:AIMS:The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14283
更新日期:2019-07-01 00:00:00
abstract:AIMS:To investigate the sporicidal mechanisms of microwave irradiation on Bacillus licheniformis spores. METHODS AND RESULTS:We measured spore viability and the release of DNA and proteins, and performed transmission electron microscopy (TEM). A microwave oven (0.5 kW) was modified to output power at 2.0 kW, which all...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04056.x
更新日期:2009-03-01 00:00:00
abstract::The achievements of a World Health Organization Anthrax Working Group, established in 1990, have been the production of two editions of guidelines on anthrax surveillance and control and the formulation of templates to assist countries in the construction of their surveillance and control programmes. The latter was ma...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00903.x
更新日期:1999-08-01 00:00:00
abstract:AIM:Physiological race determination of 143 Fusarium oxysporum f. sp. lycopersici (FOL) isolates collected along 30 years in major tomato-producing regions of Brazil. MATERIALS AND RESULTS:Physiological races were determined via root-dipping inoculation of differential tomato accessions and by the PCR-based marker sys...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14966
更新日期:2020-12-11 00:00:00
abstract:AIM:To evaluate the antifungal activity of extracts of Chamaecostus cuspidatus against Candida and Trichophyton species. METHODS AND RESULTS:Crude ethanol extracts of leaves, stems and rhizomes were prepared and evaluated for antimicrobial activity. Only the rhizomes extract (RE) showed antifungal activity but had no ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14783
更新日期:2021-01-01 00:00:00
abstract::The bactericidal activity of three bacteriocin-producing lactic acid bacteria alone and in combination with milk lactoperoxidase (LP) system activation against Listeria monocytogenes in refrigerated raw milk was studied. After 4 d at 4 degrees C, the population of L. monocytogenes in milk inoculated with bacteriocin-p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00243.x
更新日期:1997-09-01 00:00:00
abstract:AIMS:To evaluate efficacy of Saccharomyces cerevisiae fermentate prebiotic (EH) in protection of intestinal barrier integrity in rats during heat stress, to analyze the impact of heat stress and preventive treatment with EH on the structure of the gut microbiota. METHODS AND RESULTS:Two groups of rats were treated ora...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14361
更新日期:2019-10-01 00:00:00
abstract:AIMS:To compare survival of enterohaemorrhagic Escherichia coli (EHEC) strains of two clonal groups in a simulated gastric environment and to quantify the effect of storage in an acidic food, apple juice, on subsequent survival of EHEC in the simulated gastric environment. METHODS AND RESULTS:To characterize acid resi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03099.x
更新日期:2007-02-01 00:00:00
abstract:AIM:To describe the relationship between the concentration of different indicator bacteria in red meat. METHODS AND RESULTS:Enumeration data for aerobic plate count (APC), Enterobacteriaceae, coliforms and Escherichia coli biotype I were analysed from an Australia-wide survey of beef carcasses, sheep carcasses, frozen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03060.x
更新日期:2007-01-01 00:00:00
abstract::A total of 21 plant-associated Serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rDNA, PCR fingerprints using BOX prim...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01064.x
更新日期:2000-06-01 00:00:00
abstract::Sustainable economic and industrial growth is the need of the hour and it requires renewable energy resources having better performance and compatibility with existing fuel infrastructure from biological routes. Isoprenoids (C ≥ 5) can be a potential alternative due to their diverse nature and physiochemical propertie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.12871
更新日期:2015-09-01 00:00:00
abstract:AIMS:To evaluate the inhibitory effect of five structurally different imidazolium salts on the in vitro growth of plant pathogenic bacteria that belong to divergent taxonomic genera as well as their ability to reduce the severity of common bacterial blight of common bean caused by Xanthomonas axonopodis pv. phaseoli an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14575
更新日期:2020-05-01 00:00:00
abstract::The study of wild strains from natural habitats is a useful means of understanding better the heterogeneity within a species of biotechnological importance, and of obtaining atypical isolates with unknown capabilities. In the present research carried out on different Lactobacillus helveticus strains isolated from natu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00312.x
更新日期:1998-01-01 00:00:00
abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12456
更新日期:2014-06-01 00:00:00
abstract:AIMS:To study the effects of temperature, pH and yeast extract (YE) concentration on growth and exopolysaccharide (EPS) production by Propionibacterium acidi-propionici DSM 4900 cultivated on milk microfiltrate. METHODS AND RESULTS:A multifactorial approach using a Response Surface Methodology (RSM) was followed. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01310.x
更新日期:2001-05-01 00:00:00
abstract::Pseudomonas fluorescens RO98, a raw milk isolate, was inoculated into McKellar's minimal salts medium and incubated at 25 degrees C for 48 h to allow production of protease. A zinc-metalloacid protease was purified from the cell-free concentrate by anion exchange and gel filtration chromatography. The purified proteas...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01108.x
更新日期:2000-08-01 00:00:00
abstract:AIMS:To investigate the interactions of Salmonella enterica with abiotic and plant surfaces and their effect on the tolerance of the pathogen to various stressors. METHODS AND RESULTS:Salmonella strains were tested for their ability to form biofilm in various growth media using a polystyrene plate model. Strong biofil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04152.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:To investigate the effects of whey retentate from Bifidobacteria fermented milk. METHODS AND RESULTS:The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01482.x
更新日期:2001-12-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14520
更新日期:2020-03-01 00:00:00
abstract:AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02058.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To determine the effects of herbal essential oils on Trichophyton spp. growth and to evaluate the effects of Pelargonium graveolens oil and its main components citronellol and geraniol combined with ketoconazole against Trichophyton spp. METHODS AND RESULTS:Growth inhibition of six Trichophyton spp. by herbal ess...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02417.x
更新日期:2004-01-01 00:00:00
abstract:AIMS: The aim of this study was to generate temperature-sensitive Edwardsiella tarda mutant and to evaluate potential of the mutant as a vaccine in olive flounder (Paralichthys olivaceus). METHODS AND RESULTS: A temperature-sensitive E. tarda mutant was generated by replacement of alr gene promoter with cI857-λP(R) pr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05328.x
更新日期:2012-08-01 00:00:00
abstract:AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04192.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:To identify changes in the bacterial community, at the phylum level brought about by varied crop management. METHODS AND RESULTS:Next-generation sequencing methods were used to compare the taxonomic structure of the bacterial community within 24 agricultural soils managed with either organic or conventional metho...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12822
更新日期:2015-07-01 00:00:00
abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12960
更新日期:2015-12-01 00:00:00
abstract:AIMS:This study was to investigate and to characterize methicillin-resistant coagulase-positive staphylococci (MRCoPS) harboring in dogs and people associated with dogs in Thailand. METHODS AND RESULTS:Staphylococci were collected from 100 dogs, 100 dog owners, 200 small animal veterinarians and 100 people without pet...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12545
更新日期:2014-08-01 00:00:00
abstract:AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02371.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:This study was performed to investigate the effects of Megasphaera elsdenii (Me), Saccharomyces cerevisiae (SC) and lactic acid bacteria (FP - Lactobacillus fermentum plus Lactobacillus plantarum) alone or in combination on biogas production and ruminal biofermentation parameter in a heterofermenter system. METHO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14990
更新日期:2021-01-07 00:00:00
abstract::A novel antibacterial substance produced by a strain isolated from Bulgarian yellow cheese was characterized. The producer strain was identified by molecular typing to belong to the species Lactobacillus delbrueckii, which is a rare producer of bacteriocins. The inhibitory agent was heat stable and active against lact...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.853568.x
更新日期:1998-09-01 00:00:00
abstract::Self-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.2008.04024.x
更新日期:2009-04-01 00:00:00