Abstract:
AIM:Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch-blood-egg yolk-polymyxin B-trimethoprim-ceftazidime (SBYPTC) agar was established to isolate and specify the number of Bacillus cereus in food products. METHODS AND RESULTS:The effectiveness of the developed medium in selecting for B. cereus from pure cultures and food matrixes naturally contaminated by high levels of microbiota was estimated, and the results were compared with that of two commercially available MYPA and PMBA media. In pure cultures, there were no significant differences in the recoverability of B. cereus among the three media, however, SBYPTC agar showed a greater exclusivity. To examine SBYPTC performance in food, B. cereus were artificially inoculated into lettuce and potato samples with high background microbiota in two separated experiments. There were no significant differences between MYPA and PEMBA. However, SBYPTC manifested greater selectivity and exclusivity and made the differentiation easier by allowing growth of B. cereus in separated colonies and inhibiting competing microflora. CONCLUSION:Our results showed that SBYPTC has high selective properties in comparison with MYPA and PEMBA. Thus, it can be considered as a useful tool to monitor the existence and the number of B. cereus in foods especially those contaminated with high levels of microflora. SIGNIFICANCE AND IMPACT OF THE STUDY:In the food industry, SBYPTC can be employed for food quality assurance to monitor B. cereus in food products contaminated with high levels of microbiota.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Talahmeh N,Abu-Rumeileh S,Al-Razem Fdoi
10.1111/jam.14561subject
Has Abstractpub_date
2020-05-01 00:00:00pages
1440-1447issue
5eissn
1364-5072issn
1365-2672journal_volume
128pub_type
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