Chrysin protects mice from Staphylococcus aureus pneumonia.

Abstract:

AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The efficacy of chrysin against human alveolar epithelial cell injury by α-haemolysin was tested using live/dead staining or by measuring lactate dehydrogenase activity. Furthermore, we determined the protective effect of chrysin against Staph. aureus pneumonia through histopathology experiments in a mouse model. The production of α-haemolysin by Staph. aureus was inhibited when presented with an increasing subinhibitory concentration of chrysin in vitro. Consistent with this result, chrysin prevented α-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia. CONCLUSIONS:Chrysin is a potent inhibitor of α-haemolysin expression by Staph. aureus, and it conferred a significant degree of protection against Staph. aureus pneumonia. SIGNIFICANCE AND IMPACT OF STUDY:The chrysin-mediated inhibition of α-haemolysin production and protection against Staph. aureus pneumonia may offer a new strategy in combating pathogen infections.

journal_name

J Appl Microbiol

authors

Wang J,Qiu J,Dong J,Li H,Luo M,Dai X,Zhang Y,Leng B,Niu X,Zhao S,Deng X

doi

10.1111/j.1365-2672.2011.05170.x

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

1551-8

issue

6

eissn

1364-5072

issn

1365-2672

journal_volume

111

pub_type

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