Abstract:
AIM:To elucidate the effect of chrysin on α-haemolysin production by Staphylococcus aureus and protection against pneumonia in a murine model. METHODS AND RESULTS:Haemolysis, Western blot and real-time RT-PCR assays were performed to evaluate the effect of chrysin on α-haemolysin secretion by Staph. aureus. The efficacy of chrysin against human alveolar epithelial cell injury by α-haemolysin was tested using live/dead staining or by measuring lactate dehydrogenase activity. Furthermore, we determined the protective effect of chrysin against Staph. aureus pneumonia through histopathology experiments in a mouse model. The production of α-haemolysin by Staph. aureus was inhibited when presented with an increasing subinhibitory concentration of chrysin in vitro. Consistent with this result, chrysin prevented α-haemolysin-mediated cell injury and protected mice from Staph. aureus pneumonia. CONCLUSIONS:Chrysin is a potent inhibitor of α-haemolysin expression by Staph. aureus, and it conferred a significant degree of protection against Staph. aureus pneumonia. SIGNIFICANCE AND IMPACT OF STUDY:The chrysin-mediated inhibition of α-haemolysin production and protection against Staph. aureus pneumonia may offer a new strategy in combating pathogen infections.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Wang J,Qiu J,Dong J,Li H,Luo M,Dai X,Zhang Y,Leng B,Niu X,Zhao S,Deng Xdoi
10.1111/j.1365-2672.2011.05170.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
1551-8issue
6eissn
1364-5072issn
1365-2672journal_volume
111pub_type
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