Abstract:
AIMS:The goal was to study the effects of sub-minimum inhibitory concentrations (sub-MICs) of amoxicillin (AMX) on various physiological responses and virulence determinants in a commensal strain of Escherichia coli. MATERIALS AND RESULTS:The commensal strain was passaged under various sub-MICs of AMX and its effect on bacterial growth, motility, biofilm formation, expression of outer membrane proteins (OMPs) and cell adhesion was analysed. Bacterial growth was diminished at 1/2 and 1/4 MICs of AMX with significant reduction in growth rate. Using crystal violet (CV) assays and quantification of surface polysaccharides we observed strong biofilm formation, together with reduced swimming motility in E. coli at 1/2 MIC of AMX. Differential OMP expression upon AMX sub-MIC exposure coincided with enhanced cell adhesion to HT-29 cells in vitro. The results demonstrated that sub-MICs of AMX can stimulate unpredictable changes in commensal bacterial strains which can be a potent source for the propagation of antibiotic resistance. CONCLUSIONS:The study reports that AMX at 1/2 MIC significantly compromised bacterial growth and swimming motility, alongside inducing biofilm formation. This was also accompanied by upregulation of a single OMP which subsequently increased cell adhesion capabilities in E. coli at 1/2 MIC, thereby enhancing its colonization and survival abilities within the gut microsphere. SIGNIFICANCE AND IMPACT OF THE STUDY:For the first time, the effects of AMX sub-MICs on a commensal E. coli strain were described. The results corroborate on how antibiotics can act as stimulatory molecules and determine the pathogenicity of commensal bacteria in vivo that can disseminate resistance to other intestinal pathogens or microbes.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Chadha Jdoi
10.1111/jam.14987subject
Has Abstractpub_date
2021-01-02 00:00:00eissn
1364-5072issn
1365-2672pub_type
杂志文章abstract:AIMS:The objective of this study was a comprehensive characterization of physiological changes of Salmonella enterica induced by intense broad spectrum pulsed light (PL). After exposing the bacteria to this nonthermal decontamination technology on a gel surface, multiple viability parameters beyond culturability were a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13231
更新日期:2016-10-01 00:00:00
abstract:AIMS:Growth of the lactic acid bacterium (LAB) Oenococcus oeni, which is involved in malolactic fermentation during the winemaking process, is stimulated by peptides originating from yeast. In this study, we investigated the impact of peptides on O. oeni growth, peptidase activity and the expression of genes encoding t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04032.x
更新日期:2009-03-01 00:00:00
abstract:AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02055.x
更新日期:2003-01-01 00:00:00
abstract::The achievements of a World Health Organization Anthrax Working Group, established in 1990, have been the production of two editions of guidelines on anthrax surveillance and control and the formulation of templates to assist countries in the construction of their surveillance and control programmes. The latter was ma...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00903.x
更新日期:1999-08-01 00:00:00
abstract:AIMS:The aims of this study were to identify the structure of antibacterial lipopeptide (LP) produced by Bacillus amyloliquefaciens strain FJAT-2349, to analyse the effects of the culture medium and temperature on LP production and to assess the biocontrol efficiency of the LPs against tomato bacterial wilt. METHODS A...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14213
更新日期:2019-05-01 00:00:00
abstract::Bacterial isolates from bean-sprouts were screened for anti-Listeria monocytogenes bacteriocins using a well diffusion method. Thirty-four of 72 isolates inhibited the growth of L. monocytogenes Scott A. One, HPB 1688, which had the biggest inhibition zone against L. monocytogenes Scott A, was selected for subsequent ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00262.x
更新日期:1997-10-01 00:00:00
abstract:AIMS:To characterize the two-component cell lysis cassette comprised of holin (Hyb5) and endolysin (Lyb5) encoded by Lactobacillus fermentum temperate bacteriophage phiPYB5, and illustrate the potential application of Lyb5 as therapeutic agents. METHODS AND RESULTS:The hyb5-lyb5 cassette was cloned from the genome lib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03953.x
更新日期:2008-12-01 00:00:00
abstract:AIMS:To investigate the influence of avilamycin (AVM) administration and its subsequent withdrawal on the emergence and disappearance of AVM-resistant enterococci in the intestine of broiler chickens. METHODS AND RESULTS:Five chicks each of C, L and H groups were given the basal diet, the basal diet supplemented with ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03140.x
更新日期:2007-04-01 00:00:00
abstract:AIMS:To develop a suite of group-specific, rRNA-targeted oligonucleotide scissor probes for the quantitative detection of the predominant bacterial groups within the ruminal microbial community with the rRNA cleavage reaction-mediated microbial quantification method. METHODS AND RESULTS:Oligonucleotides that complemen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03443.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To obtain bacteria with PKS (polyketide synthase) genes and antimicrobial activity from sponges. METHODS AND RESULTS:Eighteen bacteria with KS (ketosynthase) genes were identified by polymerase chain reaction (PCR) screening of 98 isolates from South China Sea sponges, Stelletta tenuis, Halichondria rugosa, Dysid...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04241.x
更新日期:2009-08-01 00:00:00
abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01590.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14586
更新日期:2020-06-01 00:00:00
abstract::A number of bacterial strains used for meat fermentations were screened for proteolytic activity. A strain of Micrococcus which was found to be proteolytic was evaluated for the effects of environmental conditions on its proteolytic activity against pork myofibrillar proteins using response surface methodology. Three ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00829.x
更新日期:1999-12-01 00:00:00
abstract:AIMS:To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS:The ApcMin/+ mouse model was used to study the potential preventive effect of LGG on intestinal poly...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13386
更新日期:2017-03-01 00:00:00
abstract:AIM:Calibration of impedance measurement was performed vs the Association Françoise de Normalisation (AFNOR) MPN method with a view to rapid enumeration of Escherichia coli in live marine bivalve molluscs. METHODS AND RESULTS:Linear regression models between log10 MPN and detection time (DT) were adjusted for several ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02218.x
更新日期:2004-01-01 00:00:00
abstract::Pseudomonas fluorescens DR54 showed antagonistic properties against plant pathogenic Pythium ultimum and Rhizoctonia solani both in vitro and in planta. Antifungal activity was extractable from spent growth media, and fractionation by semi-preparative HPLC resulted in isolation of an active compound, which was identif...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00798.x
更新日期:1999-07-01 00:00:00
abstract:AIMS:Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has emerged as a severe bacterial disease of cultured shrimp. To identify the key virulence factors, two AHPND-causing V. parahaemolyticus (VpAHPND ) strains (123 and 137) and two non-VpAHPND strains (HZ56 and ATCC 17082) were select...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14922
更新日期:2020-11-05 00:00:00
abstract:AIMS:In this work, the 'hi-tech' complex biocleaning and restoration of the 14th-century fresco Triumph of Death (5·6 × 15·0 m) at the Camposanto Monumental Cemetery (Pisa, Italy) is reported. Since 2000, the restoration based on the biological cleaning of noble medieval frescoes, has been successfully utilized in this...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13913
更新日期:2018-09-01 00:00:00
abstract::The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00821.x
更新日期:2000-01-01 00:00:00
abstract:AIMS:To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis. METHODS AND RESULTS:The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples o...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04526.x
更新日期:2010-04-01 00:00:00
abstract:AIMS:Evaluation of the antibacterial activity of cultivable bacteria associated with the marine sponges Hymeniacidon perlevis and Halichondria panicea against multi-drug-resistant Staphylococcus aureus. METHODS AND RESULTS:One hundred and fourteen bacterial isolates were recovered from H. perlevis and H. panicea. Anti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14999
更新日期:2021-01-09 00:00:00
abstract::The survival of Bacteroides thetaiotaomicron strain BTX under rumen-simulating conditions was studied. Strain BTX is a recombinant variant of strain 5482 engineered for the production of high levels of xylanase, an enzyme important in the degradation of hemicellulose. Strain BTX was not inhibited by compounds present ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00152.x
更新日期:1997-06-01 00:00:00
abstract:AIMS:A number of previous studies have shown that plant extracts can inhibit formation of dental plaque. The ability of extracts of Rosmarinus officianalis L., Salvia officianalis L., unfermented cocoa, red grape seed and green tea to inhibit plaque bacteria, glucosyltransferase activity, glucan and plaque formation in...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05380.x
更新日期:2012-10-01 00:00:00
abstract:AIMS:Root rot caused by a group of fungi is a serious disease in mulberry. This study aims to identify and characterize Rhizopus oryzae and other fungal species associated with root rot of mulberry in India. METHODS AND RESULTS:Rotted root samples were collected from the mulberry gardens from four states of Southern I...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14959
更新日期:2020-12-04 00:00:00
abstract:AIMS:Poor colonization of the rhizosphere is a major constraint of seed treatment biological control. The objectives of this study were to; examine the colonization of the rhizosphere of sugar beet seedlings by selected rhizobacteria; determine the influence of the host rhizosphere and percolating water on the distribu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01523.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol-acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen-restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04668.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:Ascospores of Talaromyces macrosporus are constitutively dormant and germinate after a strong external shock, classically a heat treatment. This fungus is used as a model system to study heat resistance leading to food spoilage after pasteurization. This study evaluates the effect of high pressure on the germinati...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02133.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To investigate effects of the cationic surfactant cetyltrimethylammonium bromide (CTAB), a disinfectant, on spores of Bacillus species. METHODS AND RESULTS:The ability of CTAB to trigger release of Bacillus spores' large depot of dipicolinic acid (DPA) in a 1 : 1 chelate with Ca2+ (CaDPA), and to kill spores was ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14242
更新日期:2019-05-01 00:00:00
abstract::Currently, symbiotic rhizobia (sl., rhizobium) refer to the soil bacteria in α- and β-Proteobacteria that can induce root and/or stem nodules on some legumes and a few of nonlegumes. In the nodules, rhizobia convert the inert dinitrogen gas (N2 ) into ammonia (NH3 ) and supply them as nitrogen nutrient to the host pla...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14960
更新日期:2020-12-10 00:00:00
abstract:AIMS:To investigate the nitrogen-microbial community dynamics during composting of a mixture of nitrifying waste activated sludge (WAS) and fine organic fraction of residual household waste (RHW). To examine whether the addition of nitrifying sludge could promote ammonia oxidation and reduce ammonia emissions. METHODS...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12651
更新日期:2014-12-01 00:00:00