Abstract:
AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with the ISO method. METHODS AND RESULTS:Samples were analysed by both methods and the generated data were analysed according to ISO 17994 and showed that Pseudalert resulted in significantly higher counts and a better recovery of P. aeruginosa than the ISO method for 10 and 100 ml sample volumes. CONCLUSIONS:Pseudalert represents a significant improvement in the enumeration of P. aeruginosa from cooling tower water and related samples. The advantages of Pseudalert became apparent in a better performance, a more than 10-fold higher upper quantification limit when using Quanti-Tray/2000 and a shorter incubation time with no requirement for further confirmation, resulting in a faster reporting of results. SIGNIFICANCE AND IMPACT OF THE STUDY:The (Pseudalert/Quanti-Tray) MPN method offers a more efficient and sensitive test for enumerating P. aeruginosa from cooling tower waters, thus significantly improving management of occupational safety during cleaning and maintenance activities of cooling towers.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Spies K,Pleischl S,Sartory Ddoi
10.1111/jam.14586subject
Has Abstractpub_date
2020-06-01 00:00:00pages
1843-1850issue
6eissn
1364-5072issn
1365-2672journal_volume
128pub_type
杂志文章abstract:AIMS:A high-quality inoculum of Gluconacetobacter xylinus is important to produce bacterial cellulose (BC), a versatile biomaterial. This work aims to develop a method of preparing an inoculum of this bacterium with high cell density and without mutants. METHODS AND RESULTS:Inocula of G. xylinus ACCC 10220 without and...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13193
更新日期:2016-09-01 00:00:00
abstract:AIMS:To evaluate the relationship between the genomospecies, phenotypic profile and pathogenicity for carp of 37 motile Aeromonas strains. METHODS AND RESULTS:Aeromonas strains were identified to genomospecies level by the 16S rDNA restriction fragment length polymorphism (RFLP) method and characterized phenotypically...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01784.x
更新日期:2002-01-01 00:00:00
abstract:AIM:This study was performed to isolate and characterize novel antifungal lipopeptide from Bacillus cereus. METHODS AND RESULTS:Elucidation of its chemical structure was carried out by electrospray ionization mass spectra (ESI-MS) and Fourier transform infrared spectroscopy (FT-IR). The compound is a cyclic heptapepti...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12324
更新日期:2013-12-01 00:00:00
abstract:AIMS:To develop a protocol for environmental sampling to detect parvoviruses of dogs and cats in the environment. METHODS AND RESULTS:Environmental contamination was carried out using different dilutions of parvovirus-contaminated materials; further field samplings were performed in areas in which clinical cases of pa...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14971
更新日期:2020-12-16 00:00:00
abstract:AIMS:This study was performed to analyse the molecular characteristics of genes encoding for the major virulence factors in Bacillus anthracis vaccine strain 'Carbosap' compared with the wild B. anthracis strain, to evaluate the basis of attenuation. METHODS AND RESULTS:The molecular characteristics of the B. anthraci...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01660.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:A microarray was developed to simultaneously detect Cryptosporidium parvum, Cryptosporidium hominis, Enterococcus faecium, Bacillus anthracis and Francisella tularensis in water. METHODS AND RESULTS:A DNA microarray was designed to contain probes that specifically detected C. parvum, C. hominis, Ent. faecium, B. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12073
更新日期:2013-02-01 00:00:00
abstract:AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13311
更新日期:2017-01-01 00:00:00
abstract:AIMS:To develop species-specific monitoring techniques for rapid detection of Bacteroides and Parabacteroides inhabiting the mouse intestine by fluorescence in situ hybridization. METHODS AND RESULTS:The specificity of oligonucleotide probes was evaluated by fluorescence whole-cell hybridization. Oligonucleotide probe...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05039.x
更新日期:2011-07-01 00:00:00
abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13021
更新日期:2016-02-01 00:00:00
abstract:AIM:This study examined the antifungal activity of the combination of tyrosol and farnesol against Candida albicans and Candida glabrata in the planktonic state or forming biofilms. METHODS AND RESULTS:The effect of drug association against Candida planktonic cells was assessed by the fractional inhibitory concentrati...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13513
更新日期:2017-08-01 00:00:00
abstract:AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03018.x
更新日期:2006-11-01 00:00:00
abstract:AIMS:To apply a denaturing gradient gel electrophoretic (DGGE) method to quantify and profile individual strains during a mixed culture fermentation. METHODS AND RESULTS:DNA was extracted during the culture of lactic acid bacteria (LAB) and amplified in a multiple competitive PCR (cPCR) using general primers targeting...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02058.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To determine the mathematical kinetic rates and mechanisms of acclimated perchlorate (ClO)-reducing microbial cultures by incorporating a term to relate the inhibitory effect of high salinity during biological reduction of concentrated perchlorate solutions. METHODS AND RESULTS:Salt toxicity associated with the b...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02950.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:To assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, Thermophilin 1277, production by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277, which was produced by S. thermophilus SBT1277, showed an antimicrobial activity against several lactic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03159.x
更新日期:2007-04-01 00:00:00
abstract:AIMS:Silage is grass, preserved by fermentation and used as winter feed for cattle. The impact of a range of current grass silage preparation practices on the survival of Escherichia coli C600φ3538(Δvtx2 ::cat) and on the induction, release and infectivity of free phage were investigated. METHODS AND RESULTS:Wilted an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13431
更新日期:2017-05-01 00:00:00
abstract::A novel antibacterial substance produced by a strain isolated from Bulgarian yellow cheese was characterized. The producer strain was identified by molecular typing to belong to the species Lactobacillus delbrueckii, which is a rare producer of bacteriocins. The inhibitory agent was heat stable and active against lact...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.853568.x
更新日期:1998-09-01 00:00:00
abstract:AIM:Insect frass samples were collected from Drosophila melanogaster, Plodia interpunctella, Rhyzopertha dominica, Sitophilus granarius, Sitophilus oryzae, Sitophilus zeamais, Tribolium confusum and Tribolium castaneum to elucidate if they can be the origin of Type I sourdough micro-organisms (Lactobacillus sanfrancisc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13546
更新日期:2017-10-01 00:00:00
abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00412.x
更新日期:1998-05-01 00:00:00
abstract:AIM:Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts was studied with different compost types, storage conditions and inoculum levels. METHODS AND RESULTS:A cocktail of three E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or p...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13719
更新日期:2018-05-01 00:00:00
abstract:AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12820
更新日期:2015-07-01 00:00:00
abstract:AIMS:To investigate the antibacterial efficacy of vancomycin towards Staphylococcus aureus under aerobic and anaerobic conditions, and to assess the influence of oxygen on the duration of the post-antibiotic effect (PAE) after exposure to vancomycin. METHODS AND RESULTS:Culture-based techniques and flow cytometric mea...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01594.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02741.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:The aim of this study was to evaluate the inactivation efficiency of Listeria monocytogenes ATC(L3) C 7644 and Salmonella enterica serovar Typhimurium strain DS88 by combined treatment of hypericin (Hyp)-based photosensitization and high power pulsed light (HPPL). METHODS AND RESULTS:Cells were incubated with Hyp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05296.x
更新日期:2012-06-01 00:00:00
abstract:AIMS:To determine the transcriptional responses of Trichophyton rubrum to the artificial substance, PH11B. METHODS AND RESULTS:The broth microdilution assay for antifungal susceptibility testing of dermatophytes was used to measure the minimum inhibitory concentration (MIC) of PH11B. cDNA microarray technology and rea...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03521.x
更新日期:2007-12-01 00:00:00
abstract:AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13309
更新日期:2016-12-01 00:00:00
abstract:AIMS:To evaluate the use of relatively low levels of hydrogen peroxide vapour (HPV) for the inactivation of Bacillus anthracis spores within an indoor environment. METHODS AND RESULTS:Laboratory-scale decontamination tests were conducted using bacterial spores of both B. anthracis Ames and Bacillus atrophaeus inoculat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13284
更新日期:2016-12-01 00:00:00
abstract:AIMS:The aims of this study were to evaluate the ability of exogenous Lactobacillus acidophilus strain NCFM to survive through the human gastro-intestinal (GI) tract, and to evaluate the selectivity of Rogosa SL medium for faecal lactobacilli. METHODS AND RESULTS:The composition of the faecal lactobacilli of 10 health...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01767.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:Spodoptera exigua is a serious pest of many agricultural crops. However, the bacterial communities of S. exigua are poorly studied, particularly over their entire life cycle. We aimed to study the biodiversity of the microbiota across the life cycle of S. exigua and to provide a better and obtain insight into new ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14190
更新日期:2019-04-01 00:00:00
abstract:AIMS:To identify and characterize adhesion-associated proteins in the potential probiotic Lactobacillus fermentum BCS87. METHODS AND RESULTS:Protein suspensions obtained from the treatment of Lact. fermentum BCS87 with 1 mol 1(-1) LiCl were analysed by Western blotting using HRP-labelled porcine mucus and mucin. Two a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04368.x
更新日期:2009-12-01 00:00:00
abstract::Basil essential oils, including basil sweet linalool (BSL) and basil methyl chavicol (BMC), were screened for antimicrobial activity against a range of Gram-positive and Gram-negative bacteria, yeasts and moulds using an agar well diffusion method. Both essential oils showed antimicrobial activity against most of the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00338.x
更新日期:1998-02-01 00:00:00