Comparison of the Pseudalert™/Quanti-Tray® MPN test for the enumeration of Pseudomonas aeruginosa in cooling tower water with the ISO 16266 membrane filtration culture-based method.

Abstract:

AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with the ISO method. METHODS AND RESULTS:Samples were analysed by both methods and the generated data were analysed according to ISO 17994 and showed that Pseudalert resulted in significantly higher counts and a better recovery of P. aeruginosa than the ISO method for 10 and 100 ml sample volumes. CONCLUSIONS:Pseudalert represents a significant improvement in the enumeration of P. aeruginosa from cooling tower water and related samples. The advantages of Pseudalert became apparent in a better performance, a more than 10-fold higher upper quantification limit when using Quanti-Tray/2000 and a shorter incubation time with no requirement for further confirmation, resulting in a faster reporting of results. SIGNIFICANCE AND IMPACT OF THE STUDY:The (Pseudalert/Quanti-Tray) MPN method offers a more efficient and sensitive test for enumerating P. aeruginosa from cooling tower waters, thus significantly improving management of occupational safety during cleaning and maintenance activities of cooling towers.

journal_name

J Appl Microbiol

authors

Spies K,Pleischl S,Sartory D

doi

10.1111/jam.14586

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

1843-1850

issue

6

eissn

1364-5072

issn

1365-2672

journal_volume

128

pub_type

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