Cytotoxicity and apoptosis induction of some selected marine bacteria metabolites.


AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find the potential active strain with apoptosis mechanism, a battery of apoptosis assays, including AO/EB staining, TUNEL assay (terminal-deoxynucleotidyl transferase mediated nick end labelling), gel electrophoresis and flow cytometry, were used to determine whether apoptosis was involved in HeLa cell cytotoxicity of marine bacterial extracts. The results indicated that four strains could induce cell shrinkage, cell membrane blebbing, formation of apoptotic body and DNA fragmentation. CONCLUSIONS:Crude extracts of 12 of 153 strains of marine bacteria showed cytotoxic effects with ID50 ranged from 77.20 to 199.84 microg ml(-1), in which eight strains of bacteria were associated bacteria. The metabolites in the strains of QD1-2, NJ6-3-1, NJ1-1-1 and SS6-4 were able to induce HeLa cells apoptosis. Furthermore, the assessment by flow cytometry indicated that the hypodiploid apoptotic cells increased in a time-dependent manner, suggesting that induced apoptosis occurred from 24 h to 48 h after the extracts treatment. SIGNIFICANCE AND IMPACT OF THE STUDY:Our results suggested that the compounds from fermentation in these four marine bacterial strains could be candidates for developing apoptosis specific anti-tumour agents with lower toxicity. This study indicated that associated marine bacteria could be good source to find cytotoxic metabolites, and some cytotoxic marine bacterial metabolites could have apoptosis mechanisms.


J Appl Microbiol


Lin J,Yan XJ,Zheng L,Ma HH,Chen HM




Has Abstract


2005-01-01 00:00:00














  • Comparison of the antibiotic resistance between genetically diverse and toxigenic Bacillus cereus sensu lato from milk, pepper and natural habitats.

    abstract:AIMS:Bacillus cereus sensu lato is a complex group of closely related bacteria, which are commonly present in the natural environment and food products. These organisms may cause food poisoning and spoilage as well as opportunistic infections. Thus far, their resistance to selected antibiotics has been explored only in...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Bartoszewicz M,Czyżewska U

    更新日期:2020-07-21 00:00:00

  • Co-overexpression of lmbW and metK led to increased lincomycin A production and decreased byproduct lincomycin B content in an industrial strain of Streptomyces lincolnensis.

    abstract:AIMS:To improve lincomycin A production and decrease the content of byproduct lincomycin B in an industrial lincomycin-producing strain. METHODS AND RESULTS:The in silico analysis indicated that LmbW could be involved in propylproline biosynthesis of lincomyin A. In this study, we constructed an lmbW deletion mutant a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Pang AP,Du L,Lin CY,Qiao J,Zhao GR

    更新日期:2015-10-01 00:00:00

  • Spelt and emmer flours: characterization of the lactic acid bacteria microbiota and selection of mixed starters for bread making.

    abstract:AIMS:This study aimed at characterizing the lactic acid bacteria microbiota and selecting mixed endogenous starters to be used for sourdough fermentation of spelt or emmer flours. METHODS AND RESULTS:Identification of lactic acid bacteria was carried out by partial sequencing of the 16S rRNA, recA, 16S/23S rRNA spacer...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Coda R,Nionelli L,Rizzello CG,De Angelis M,Tossut P,Gobbetti M

    更新日期:2010-03-01 00:00:00

  • Production of massoia lactone by Aureobasidium pullulans YTP6-14 isolated from the Gulf of Thailand and its fragrant biosurfactant properties.

    abstract:AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Luepongpattana S,Thaniyavarn J,Morikawa M

    更新日期:2017-12-01 00:00:00

  • Functional analysis of the biosynthetic gene cluster required for immunity and secretion of a novel Lactococcus-specific bacteriocin, lactococcin Z.

    abstract:AIMS:Characterization of the biosynthesis (secretion and immunity) of lactococcin Z. METHODS AND RESULTS:Lactococcin Z is produced by Lactococcus lactisQU 7. DNA sequence analysis revealed that the lactococcin Z gene cluster (c. 5·1 kb) includes four genes encoding putative biosynthetic proteins, LczB (self-immunity p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Daba GM,Ishibashi N,Zendo T,Sonomoto K

    更新日期:2017-11-01 00:00:00

  • Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894.

    abstract:AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Wang L,Hu X,Tao G,Wang X

    更新日期:2012-05-01 00:00:00

  • Specific detection of Escherichia coli isolated from water samples using polymerase chain reaction targeting four genes: cytochrome bd complex, lactose permease, beta-D-glucuronidase, and beta-D-galactosidase.

    abstract:AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Horakova K,Mlejnkova H,Mlejnek P

    更新日期:2008-10-01 00:00:00

  • Most probable number methodology for quantifying dilute concentrations and fluxes of Salmonella in surface waters.

    abstract:AIMS:To better understand and manage the fate and transport of Salmonella in agricultural watersheds, we developed a culture-based, five tube-four dilution most probable number (MPN) method for enumerating dilute densities of Salmonella in environmental waters. METHODS AND RESULTS:The MPN method was a combination of a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Jenkins MB,Endale DM,Fisher DS

    更新日期:2008-06-01 00:00:00

  • Solar UV reduces Cryptosporidium parvum oocyst infectivity in environmental waters.

    abstract:AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: King BJ,Hoefel D,Daminato DP,Fanok S,Monis PT

    更新日期:2008-05-01 00:00:00

  • A disclosure gel for visual detection of live Bacillus anthracis spores.

    abstract:AIMS:To develop a gel formulation to trigger a visual signal for rapid disclosure of the location and extent of surface contamination with viable Bacillus anthracis spores. METHODS AND RESULTS:Methylumbelliferyl-α-d-glucopyranoside was combined with hyaluronic acid to produce a gel that could be applied to a surface a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Robinson CV,Bishop AH

    更新日期:2019-06-01 00:00:00

  • Selective method for identification and quantification of Bifidobacterium animalis subspecies lactis BB-12 (BB-12) from the gastrointestinal tract of healthy volunteers ingesting a combination probiotic of BB-12 and Lactobacillus rhamnosus GG.

    abstract:AIM:To develop a novel validated method for the isolation of Bifidobacterium animalis ssp. lactis BB-12 (BB-12) from faecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. METHODS AND RESULTS:A novel method for isolating and ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,随机对照试验


    authors: Poutsiaka DD,Mahoney IJ,McDermott LA,Stern LL,Thorpe CM,Kane AV,Baez-Giangreco C,McKinney J,Davidson LE,Leyva R,Goldin B,Snydman DR

    更新日期:2017-05-01 00:00:00

  • Evaluation of group-specific, 16S rRNA-targeted scissor probes for quantitative detection of predominant bacterial populations in dairy cattle rumen.

    abstract:AIMS:To develop a suite of group-specific, rRNA-targeted oligonucleotide scissor probes for the quantitative detection of the predominant bacterial groups within the ruminal microbial community with the rRNA cleavage reaction-mediated microbial quantification method. METHODS AND RESULTS:Oligonucleotides that complemen...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Uyeno Y,Sekiguchi Y,Tajima K,Takenaka A,Kurihara M,Kamagata Y

    更新日期:2007-11-01 00:00:00

  • Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells.

    abstract:AIMS:The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. METHOD AND RESULTS:We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic acti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Jeong SY,Park SY,Kim YH,Kim M,Lee SJ

    更新日期:2008-03-01 00:00:00

  • Development of multiplex real-time PCR for simultaneous detection of three Potyviruses in tobacco plants.

    abstract:AIMS:To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus (TEV), Potato virus Y (PVY) and Tobacco vein banding mosaic virus (TVBMV). METHODS AND RESULTS:Specific primer and probe combinations for TEV and TVBMV were developed from the coa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Dai J,Peng H,Chen W,Cheng J,Wu Y

    更新日期:2013-02-01 00:00:00

  • Inhibition of Bacillus licheniformis LMG 19409 from ropy cider by enterocin AS-48.

    abstract:AIMS:To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. METHODS AND RESULTS:Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Grande MJ,Lucas R,Abriouel H,Valdivia E,Ben Omar N,Maqueda M,Martínez-Cañamero M,Gálvez A

    更新日期:2006-08-01 00:00:00

  • Inhibition of multidrug-resistant Staphylococci by sodium chlorate and select nitro- and medium chain fatty acid compounds.

    abstract:AIMS:Determine the antimicrobial effects of 5 μmol ml-1 sodium chlorate, 9 μmol ml-1 nitroethane or 2-nitropropanol as well as lauric acid, myristic acid and the glycerol ester of lauric acid Lauricidin® , each at 5 mg ml-1 , against representative methicillin-resistant staphylococci, important mastitis- and opportunis...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Božić A,Anderson RC,Arzola-Alvarez C,Ruiz-Barrera O,Corral-Luna A,Castillo-Castillo Y,Arzola-Rubio A,Poole TL,Harvey RB,Hume ME,Beier RC,Nisbet DJ

    更新日期:2019-05-01 00:00:00

  • Mechanisms of killing of spores of Bacillus subtilis by dimethyldioxirane.

    abstract:AIMS:To determine the mechanisms of Bacillus subtilis spore resistance to and killing by a novel sporicide, dimethyldioxirane (DMDO) that was generated in situ from acetone and potassium peroxymonosulfate at neutral pH. METHODS AND RESULTS:Spores of B. subtilis were effectively killed by DMDO. Rates of killing by DMDO...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Paul M,Atluri S,Setlow B,Setlow P

    更新日期:2006-11-01 00:00:00

  • Pathogenic potential of Escherichia coli O157 and O26 isolated from young Belgian dairy calves by recto-anal mucosal swab culturing.

    abstract:AIMS:The purpose of this study was to investigate the occurrence of Escherichia coli O157 and O26 on Belgian dairy cattle farms, the presence of virulence genes in the confirmed isolates and the association of E. coli O26 presence with calf diarrhoea. METHODS AND RESULTS:In total, 233 recto-anal mucosal swabs (RAMS) w...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Engelen F,Thiry D,Devleesschauwer B,Heyndrickx M,Mainil J,De Zutter L,Cox E

    更新日期:2020-10-26 00:00:00

  • Cloning and study of the expression of a novel cry1Ia-type gene from Bacillus thuringiensis subsp. kurstaki.

    abstract:AIMS:Cloning and expression of a new cry1Ia-type gene of Bacillus thuringiensis. METHODS AND RESULTS:PCR amplification, using gene cry1I-specific primers revealed the presence of such a gene in the strain BNS3 of Bacillus thuringiensis subsp. kurstaki. The cloning and sequencing from BNS3 of the cry1Ia-type gene, call...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Tounsi S,Zouari N,Jaoua S

    更新日期:2003-01-01 00:00:00

  • Survival of Campylobacter jejuni and Escherichia coli in groundwater during prolonged starvation at low temperatures.

    abstract:AIMS:To evaluate the survival of Campylobacter jejuni relative to that of Escherichia coli in groundwater microcosms varying in nutrient composition. METHODS AND RESULTS:Studies were conducted in groundwater and deionized water incubated for up to 470 days at 4 degrees C. Samples were taken for culturable and total ce...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Cook KL,Bolster CH

    更新日期:2007-09-01 00:00:00

  • Assessment of adenovirus, hepatitis A virus and rotavirus presence in environmental samples in Florianopolis, South Brazil.

    abstract:AIMS:To assess the presence of human adenovirus (HAdV), hepatitis A (HAV) virus and rotavirus A (RV-A) in environmental samples from the Southern region of Brazil and to provide viral contamination data for further epidemiological studies and governmental actions. METHODS AND RESULTS:Water samples from various sources...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Rigotto C,Victoria M,Moresco V,Kolesnikovas CK,Corrêa AA,Souza DS,Miagostovich MP,Simões CM,Barardi CR

    更新日期:2010-12-01 00:00:00

  • Shoe soles as a potential vector for pathogen transmission: a systematic review.

    abstract::Shoe soles are possible vectors for infectious diseases. Although studies have been performed to assess the prevalence of infectious pathogens on shoe soles and decontamination techniques, no systematic review has ever occurred. The aim of this study was to perform a systematic review of the literature to determine th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审


    authors: Rashid T,VonVille HM,Hasan I,Garey KW

    更新日期:2016-11-01 00:00:00

  • Widespread distribution of the bacteriocin-producing lactic acid cocci in Miso-paste products.

    abstract:AIMS:A survey was conducted on the ecological distribution of bacteriocin-producing lactic acid bacteria in Miso-pastes, a typical fermented food in Japan. METHODS AND RESULTS Nine Miso-pastes were sampled for isolation of bacteriocin-producers. Almost all isolated enterococcal strains produced bacteriocins but no isol...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Onda T,Yanagida F,Uchimura T,Tsuji M,Ogino S,Shinohara T,Yokotsuka K

    更新日期:2002-01-01 00:00:00

  • The adaptive response of Escherichia coli O157 in an environment with changing pH.

    abstract:AIMS:To predict and validate survival of non-acid adapted Escherichia coli O157 in an environment mimicking the human stomach. METHODS AND RESULTS:Survival was predicted mathematically from inactivation rates at various, but constant pH values. Predictions were subsequently validated experimentally in a pH-controlled ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: de Jonge R,Takumi K,Ritmeester WS,van Leusden FM

    更新日期:2003-01-01 00:00:00

  • Salt stress induces programmed cell death in prokaryotic organism Anabaena.

    abstract:AIMS:Our main interest is to check if programmed cell death (PCD) can occur in prokaryotic algae and if the morphological and biochemical features of PCD are conserved. METHODS AND RESULTS:Using TUNEL labelling, fluorescence and light microscopy and DNA gel electrophoresis, we found that cell death with features simil...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Ning SB,Guo HL,Wang L,Song YC

    更新日期:2002-01-01 00:00:00

  • Combined effect of bacteriocin-producing lactic acid bacteria and lactoperoxidase system activation on Listeria monocytogenes in refrigerated raw milk.

    abstract::The bactericidal activity of three bacteriocin-producing lactic acid bacteria alone and in combination with milk lactoperoxidase (LP) system activation against Listeria monocytogenes in refrigerated raw milk was studied. After 4 d at 4 degrees C, the population of L. monocytogenes in milk inoculated with bacteriocin-p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Rodríguez E,Tomillo J,Nuñez M,Medina M

    更新日期:1997-09-01 00:00:00

  • Biofilm problems in dental unit water systems and its practical control.

    abstract::Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审


    authors: Coleman DC,O'Donnell MJ,Shore AC,Russell RJ

    更新日期:2009-05-01 00:00:00

  • Identification of antimicrobial metabolites produced by a potential biocontrol Actinomycete strain A217.

    abstract:AIMS:To extract and identify the metabolites of strain A217 as well as its antifungal spectrum and control effect on various plant pathogens. METHODS AND RESULTS:Strain A217 was identified as a Streptomyces sp. which was most similar to Streptomyces lienomycini. An antimicrobial spectrum test indicated that strain A21...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: He H,Hao X,Zhou W,Shi N,Feng J,Han L

    更新日期:2020-04-01 00:00:00

  • Rumen bacteria at work: bioaugmentation strategies to enhance biogas production from cow manure.

    abstract:AIMS:To investigate the effects of different bioaugmentation strategies for enhancing the biogas production from cow manure and evaluate microbial community patterns. METHODS AND RESULTS:Co-inoculation with cow rumen fluid and cow rumen-derived enriched microbial consortia was evaluated in anaerobic batch tests at 36°...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Ozbayram EG,Akyol Ç,Ince B,Karakoç C,Ince O

    更新日期:2018-02-01 00:00:00

  • The vegetative compatibility group to which the US biocontrol agent Aspergillus flavus AF36 belongs is also endemic to Mexico.

    abstract:AIMS:To assess frequencies of the Aspergillus flavus atoxigenic vegetative compatibility group (VCG) YV36, to which the biocontrol agent AF36 belongs, in maize-growing regions of Mexico. METHODS AND RESULTS:Over 3500 A. flavus isolates recovered from maize agroecosystems in four states of Mexico during 2005 through 20...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章


    authors: Ortega-Beltran A,Grubisha LC,Callicott KA,Cotty PJ

    更新日期:2016-04-01 00:00:00