Abstract:
AIMS:To investigate the effect of various single nutrient deficiencies on poly-beta-hydroxybutyrate (PHB) biosynthesis in a methane-utilizing mixed culture (dominant species Methylocystis sp. GB 25 DSM 7674). METHODS AND RESULTS:Poly-beta-hydroxybutyrate accumulation experiments were performed in 7 and 70 l bioreactors and initiated by potassium, sulfur or iron deficiency. After 24 h the PHB content reached levels of 33.6%, 32.6% and 10.4% respectively. Interestingly a polymer with an ultra-high average-weight molecular weight (M(w)) of 3.1 MDa was accumulated under potassium-limited conditions. When sulfur and iron were lacking M(w) were lower by 20.6 and 41.6%. Potassium-deficiency experiments were furthermore characterized by a maximum specific PHB formation rate 0.08 g g(-1)residual biomass (R) h(-1) and a yield coefficient of 0.45 g PHB g(-1) CH(4). CONCLUSIONS:Biosynthesis of an ultra-high M(w) PHB in a methane-utilizing mixed culture can be induced by potassium deficiency. SIGNIFICANCE AND IMPACT OF THE STUDY:This study greatly extends the knowledge in the field of bacterial biopolymer formation with gaseous substrates. The special system used here combines the use of methane a low-cost substrate available from natural and renewable sources with the possibility of employing a mixed-culture in an open system for the synthesis of a high-value product.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Helm J,Wendlandt KD,Jechorek M,Stottmeister Udoi
10.1111/j.1365-2672.2008.03831.xsubject
Has Abstractpub_date
2008-10-01 00:00:00pages
1054-61issue
4eissn
1364-5072issn
1365-2672pii
JAM3831journal_volume
105pub_type
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