Potassium deficiency results in accumulation of ultra-high molecular weight poly-beta-hydroxybutyrate in a methane-utilizing mixed culture.

Abstract:

AIMS:To investigate the effect of various single nutrient deficiencies on poly-beta-hydroxybutyrate (PHB) biosynthesis in a methane-utilizing mixed culture (dominant species Methylocystis sp. GB 25 DSM 7674). METHODS AND RESULTS:Poly-beta-hydroxybutyrate accumulation experiments were performed in 7 and 70 l bioreactors and initiated by potassium, sulfur or iron deficiency. After 24 h the PHB content reached levels of 33.6%, 32.6% and 10.4% respectively. Interestingly a polymer with an ultra-high average-weight molecular weight (M(w)) of 3.1 MDa was accumulated under potassium-limited conditions. When sulfur and iron were lacking M(w) were lower by 20.6 and 41.6%. Potassium-deficiency experiments were furthermore characterized by a maximum specific PHB formation rate 0.08 g g(-1)residual biomass (R) h(-1) and a yield coefficient of 0.45 g PHB g(-1) CH(4). CONCLUSIONS:Biosynthesis of an ultra-high M(w) PHB in a methane-utilizing mixed culture can be induced by potassium deficiency. SIGNIFICANCE AND IMPACT OF THE STUDY:This study greatly extends the knowledge in the field of bacterial biopolymer formation with gaseous substrates. The special system used here combines the use of methane a low-cost substrate available from natural and renewable sources with the possibility of employing a mixed-culture in an open system for the synthesis of a high-value product.

journal_name

J Appl Microbiol

authors

Helm J,Wendlandt KD,Jechorek M,Stottmeister U

doi

10.1111/j.1365-2672.2008.03831.x

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

1054-61

issue

4

eissn

1364-5072

issn

1365-2672

pii

JAM3831

journal_volume

105

pub_type

杂志文章
  • Isolation and identification of synthetic pyrethroid-degrading bacteria.

    abstract:AIMS:To isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (SPs) in sheep dips. METHODS AND RESULTS:SP-degrading bacteria were isolated from a mixed soil sample consisting of garden soil and soils from farms where SPs had been used. The two largest in size were then iden...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01558.x

    authors: Grant RJ,Daniell TJ,Betts WB

    更新日期:2002-01-01 00:00:00

  • Comparison of the Pseudalert™/Quanti-Tray® MPN test for the enumeration of Pseudomonas aeruginosa in cooling tower water with the ISO 16266 membrane filtration culture-based method.

    abstract:AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14586

    authors: Spies K,Pleischl S,Sartory D

    更新日期:2020-06-01 00:00:00

  • Improving qPCR methodology for detection of foaming bacteria by analysis of broad-spectrum primers and a highly specific probe for quantification of Nocardia spp. in activated sludge.

    abstract:AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13311

    authors: Asvapathanagul P,Olson BH

    更新日期:2017-01-01 00:00:00

  • Genetically modified bacteriophages in applied microbiology.

    abstract::Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13207

    authors: Bárdy P,Pantůček R,Benešík M,Doškař J

    更新日期:2016-09-01 00:00:00

  • Interleukin-8 gene expression and apoptosis induced by Salmonella Typhimurium in the presence of Bacillus probiotics in the epithelial cell.

    abstract:AIMS:This study aimed to evaluate the effects of three Bacillus probiotics on Salmonella Typhimurium, and interleukin-8 (IL-8) gene expression in the co-culture of the Bacillus and the pathogen in vitro. METHODS AND RESULTS:Bacillus subtilis, Bacillus indicus and Bacillus coagulans were initially turned to spore and h...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14898

    authors: Kawarizadeh A,Pourmontaseri M,Farzaneh M,Hosseinzadeh S,Ghaemi M,Tabatabaei M,Pourmontaseri Z,Pirnia MM

    更新日期:2020-10-15 00:00:00

  • Morphological and phenotypical characterization of Bacillus sporothermodurans.

    abstract:AIMS:Enumeration of resistant bacteria in ultra-high temperature (UHT) treated milk; morphological characterization and phenotyping of resistant strains by traditional and nontraditional methods and their identification by molecular biology. METHODS AND RESULTS:Modified standard plate count agar (PCA) and modified bra...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02371.x

    authors: Montanari G,Borsari A,Chiavari C,Ferri G,Zambonelli C,Grazia L

    更新日期:2004-01-01 00:00:00

  • Microbiological composition of raw milk from selected farms in the Camembert region of Normandy.

    abstract::Raw milk from 27 farms was sampled over 6 months for listerias, salmonellas, Yersinia enterocolitica and campylobacters. Total bacterial counts and somatic cell counts were measured. Lactococci, lactobacilli, dextran-producing leuconostocs, Brevibacterium linens, yeasts and moulds, Staphylococcus aureus and other Micr...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00166.x

    authors: Desmasures N,Bazin F,Guéguen M

    更新日期:1997-07-01 00:00:00

  • Prevalence, phenotypic traits and molecular characterization of emetic toxin-producing Bacillus cereus strains isolated from human stools in Korea.

    abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05277.x

    authors: Chon JW,Kim JH,Lee SJ,Hyeon JY,Song KY,Park C,Seo KH

    更新日期:2012-05-01 00:00:00

  • Continuous culture as a tool for investigating the growth physiology of heterotrophic hyperthermophiles and extreme thermoacidophiles.

    abstract::Although there is great scientific and technological interest in examining the physiology and bioenergetics of microorganisms from extreme environments, difficulties encountered in their cultivation and lack of genetic systems hampers the investigation of these issues. As such, we have adapted methods for continuous c...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.1998.tb05290.x

    authors: Rinker KD,Han CJ,Kelly RM

    更新日期:1998-12-01 00:00:00

  • In vitro antibacterial activity and antifungal mode of action of flocculosin, a membrane-active cellobiose lipid.

    abstract:AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04280.x

    authors: Mimee B,Pelletier R,Bélanger RR

    更新日期:2009-09-01 00:00:00

  • Identification and virulence of Aeromonas dhakensis, Pseudomonas mosselii and Microbacterium paraoxydans isolated from Nile tilapia, Oreochromis niloticus, cultivated in Mexico.

    abstract:AIMS:To identify bacterial pathogens of diseased NiIe tilapia Oreochromis niloticus and determine their virulence. METHODS AND RESULTS:Sixteen bacterial isolates were recovered from diseased Nile tilapias (O. niloticus) reared in floating cages in Adolfo Lopez Mateos (ALM), Sanalona and Dique IV dams in Sinaloa, Mexic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12280

    authors: Soto-Rodriguez SA,Cabanillas-Ramos J,Alcaraz U,Gomez-Gil B,Romalde JL

    更新日期:2013-09-01 00:00:00

  • Differential and synergistic effects of xylanase and laccase mediator system (LMS) in bleaching of soda and waste pulps.

    abstract:AIMS:Investigation of waste pulps and soda pulp bleaching with xylanase (X) and laccase mediator system (LMS) alone and in conjunction (one after the other) (XLMS). METHODS AND RESULTS:Soda and different grades of waste pulp fibres [used for making three-layered duplex sheets - top layer (TL), protective layer (PL) an...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03251.x

    authors: Kapoor M,Kapoor RK,Kuhad RC

    更新日期:2007-08-01 00:00:00

  • The adaptive response of Escherichia coli O157 in an environment with changing pH.

    abstract:AIMS:To predict and validate survival of non-acid adapted Escherichia coli O157 in an environment mimicking the human stomach. METHODS AND RESULTS:Survival was predicted mathematically from inactivation rates at various, but constant pH values. Predictions were subsequently validated experimentally in a pH-controlled ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01865.x

    authors: de Jonge R,Takumi K,Ritmeester WS,van Leusden FM

    更新日期:2003-01-01 00:00:00

  • Effects of temperature on metal tolerance and the accumulation of Zn and Pb by metal-tolerant fungi isolated from urban runoff treatment wetlands.

    abstract:AIMS:To investigate the ability of two fungi to accumulate Zn and Pb, the effect of temperature on their metal tolerance and possible mechanisms involved in metal accumulation. METHODS AND RESULTS:Beauveria bassiana and Rhodotorula mucilaginosa isolated from constructed wetlands receiving urban runoff were grown in mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04082.x

    authors: Purchase D,Scholes LN,Revitt DM,Shutes RB

    更新日期:2009-04-01 00:00:00

  • Validation of a real-time PCR for Haemophilus parasuis.

    abstract:AIMS:To validate a real-time PCR test for the diagnosis of Glässer's disease, a major pig disease caused by Haemophilus parasuis. METHODS AND RESULTS:The specificity of a real-time PCR amplifying the inf B gene was validated with 68 H. parasuis isolates and 36 strains of closely related species. As well, 239 samples o...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04526.x

    authors: Turni C,Pyke M,Blackall PJ

    更新日期:2010-04-01 00:00:00

  • The regulatory effects of whey retentate from bifidobacteria fermented milk on the microbiota of the Simulator of the Human Intestinal Microbial Ecosystem (SHIME).

    abstract:AIMS:To investigate the effects of whey retentate from Bifidobacteria fermented milk. METHODS AND RESULTS:The simulator of the human intestinal microbial ecosystem (SHIME) was used. The composition of the microbiota and its metabolic activities were analysed. Changes in the microbial composition became apparent within...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01482.x

    authors: Meddah AT,Yazourh A,Desmet I,Risbourg B,Verstraete W,Romond MB

    更新日期:2001-12-01 00:00:00

  • Modified semi-continuous fermentation for resuscitating nongrowing cells during high-temperature gluconic acid production by Acetobacter senegalensis.

    abstract:AIMS:The formation of metabolically inactive and nongrowing cells is an inevitable by-product of intensive fermentation. This study investigated whether co-feeding can be used to resuscitate nongrowing Acetobacter senegalensis cells to enable them to produce gluconic acid in successive fermentation runs at 38°C. METHO...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14371

    authors: Zarmehrkhorshid R,Shafiei R,Delvigne F

    更新日期:2019-10-01 00:00:00

  • Characterization of a bovine isolate Lactobacillus mucosae DPC 6426 which produces an exopolysaccharide composed predominantly of mannose residues.

    abstract:AIMS:To characterize Lactobacillus strains with EPS-producing ability compared with non-EPS-producing lactobacilli of the same species for technological performance including simulated gastrointestinal tract (GIT) conditions. METHODS AND RESULTS:Characterization of EPS-producing Lactobacillus mucosae DPC 6426 in detai...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12542

    authors: London LE,Price NP,Ryan P,Wang L,Auty MA,Fitzgerald GF,Stanton C,Ross RP

    更新日期:2014-08-01 00:00:00

  • Flow cytometry: a versatile technology for specific quantification and viability assessment of micro-organisms in multistrain probiotic products.

    abstract:AIMS:Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but nonculturable (VBNC) micro-organisms and nonviable cells. The aim of the study was to evaluate flow cytometry as a novel me...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13666

    authors: Chiron C,Tompkins TA,Burguière P

    更新日期:2018-02-01 00:00:00

  • The effect of medicated diets and level of feeding on caecal microbiota of lactating rabbit does.

    abstract:AIMS:To study the effect of the type of antibiotic used in medicated diets against pathogens and the feeding level on the microbial biodiversity in the rabbit caecum. METHODS AND RESULTS:Three groups of eight does were given a diet unsupplemented (NAB) or with 100 ppm of bacitracin (BAC) or tiamulin (TIA). Litter size...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03309.x

    authors: Abecia L,Fondevila M,Balcells J,Lobley GE,McEwan NR

    更新日期:2007-10-01 00:00:00

  • Three-percent sucrose acts as a thermostabilizer for cell-adapted foot-and-mouth disease virus without any negative effect on viral growth.

    abstract:AIMS:As cell-adapted foot-and-mouth disease virus (FMDV) with H56R mutation in VP3 has reduced thermostability, this study aimed to investigate the effect of thermostabilizers on cell-adapted FMDV for vaccine production. METHODS AND RESULTS:We examined the effect of 3% sucrose, 10% (or 25%) glycerol or 10% FBS on cell...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14565

    authors: Hwang JH,Moon Y,Lee G,Kim MY,Lee KN,Park JH,Lee M,Kim B,Kim SM

    更新日期:2020-05-01 00:00:00

  • Use of enzymatic methods for rapid enumeration of coliforms in freshwaters.

    abstract::Rapid enumeration methods based on the enzymatic hydrolysis of 4-methylumbelliferyl-beta-D-galactoside and 4-methylumbelliferyl-beta-D-glucuronide were optimized for freshwaters. The enzymes beta-D-galactosidase (GALase) and beta-D-glucuronidase (GLUase) were shown to be already induced in freshwaters when tested, res...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00977.x

    authors: George I,Petit M,Servais P

    更新日期:2000-03-01 00:00:00

  • Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis.

    abstract::Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01177.x

    authors: Ishii K,Fukui M,Takii S

    更新日期:2000-11-01 00:00:00

  • Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China.

    abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12456

    authors: Li D,Gu AZ,He M

    更新日期:2014-06-01 00:00:00

  • Effect of biochanin A on corn grain (Zea mays) fermentation by bovine rumen amylolytic bacteria.

    abstract:AIMS:The objective was to determine the effect of biochanin A (BCA), an isoflavone produced by red clover (Trifolium pratense L.), on corn fermentation by rumen micro-organisms. METHODS AND RESULTS:When bovine rumen bacterial cell suspensions (n = 3) were incubated (24 h, 39°C) with ground corn, amylolytic bacteria in...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13397

    authors: Harlow BE,Flythe MD,Aiken GE

    更新日期:2017-04-01 00:00:00

  • Linezolid-resistant MRSA-CC398 carrying the cfr gene, and MRSA-CC9 isolates from pigs with signs of infection in Spain.

    abstract:AIMS:To perform the molecular characterization of 23 Staphylococcus aureus isolates from pigs with signs of infections recovered in Spanish farms during 2018-2019. METHODS AND RESULTS:The antimicrobial resistance pattern and virulence profile were determined. The molecular typing was performed by different molecular t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14988

    authors: Ruiz-Ripa L,Bellés-Bellés A,Fernández-Fernández R,García M,Vilaró A,Zarazaga M,Torres C

    更新日期:2021-01-01 00:00:00

  • Automated dead-end ultrafiltration of large volume water samples to enable detection of low-level targets and reduce sample variability.

    abstract:AIMS:A Portable Multi-use Automated Concentration System (PMACS) concentrates micro-organisms from large volumes of water through automated dead-end ultrafiltration and backflushing. The ability to detect microbial targets from ground, surface and cooling tower waters collected using standard methods was compared with ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05345.x

    authors: Leskinen SD,Kearns EA,Jones WL,Miller RS,Bevitas CR,Kingsley MT,Brigmon RL,Lim DV

    更新日期:2012-08-01 00:00:00

  • Expression of Aeromonas caviae polyhydroxyalkanoate synthase gene in Burkholderia sp. USM (JCM15050) enables the biosynthesis of SCL-MCL PHA from palm oil products.

    abstract:AIMS:Burkholderia sp. USM (JCM15050) isolated from oil-polluted wastewater is capable of utilizing palm oil products and glycerol to synthesize poly(3-hydroxybutyrate) [P(3HB)]. To confer the ability to produce polymer containing 3-hydroxyhexanoate (3HHx), plasmid (pBBREE32d13) harbouring the polyhydroxyalkanoate (PHA)...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05189.x

    authors: Chee JY,Lau NS,Samian MR,Tsuge T,Sudesh K

    更新日期:2012-01-01 00:00:00

  • Enrichment and identification of bacteria capable of reducing chemical oxygen demand of anaerobically treated molasses spent wash.

    abstract:AIMS:The aim of this study was to isolate and identify bacterial strains capable of using recalcitrant compounds of molasses spent wash as sole carbon source from the soils of abandoned sites of distillery effluent discharge and characterize their ability of reducing the chemical oxygen demand (COD) of the spent wash. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02289.x

    authors: Ghosh M,Verma SC,Mengoni A,Tripathi AK

    更新日期:2004-01-01 00:00:00

  • Evidence of gushing induction by Penicillium oxalicum proteins.

    abstract:AIMS:The aim of this study was to elucidate whether grape-associated fungi exert an influence on gushing by their production of surface-active compounds. METHODS AND RESULTS:In preliminary experiments, 58 grape-associated isolates of species within Penicillium and Aspergillus genera were tested for their ability to mo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13366

    authors: Vogt EI,Kupfer VM,Vogel RF,Niessen L

    更新日期:2017-03-01 00:00:00