Abstract:
AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin-producing Microcystis varied across sampling periods and locations, which were assessed using qPCR with primers specific to Microcystis 16S rDNA and mcyA genes. The 16S rDNA from two lakes were relatively diverse. However, mcyA genes were rather conservative and were >97% identical to reference sequences. The highly positive correlations between mcyA and microcystin presence (r = 0·671 in Tai; r = 0·799 in Yang-cheng) suggested that mcyA can be used as a good biomarker for microcystin productions. CONCLUSION:The results demonstrated that Microcystis were genetically diverse between these conjunctive lakes; however, mcyA genes were relatively conservative in two lakes. Quantifying mcyA by qPCR was an efficient tool for monitoring toxic Microcystis. SIGNIFICANCE AND IMPACT OF THE STUDY:This study has improved our understanding of observable differences within and between each lake on spatial and temporal scales. And the discovery of new mcyA sequences in natural water enriched the understanding of phylogenetic diversity of Microcystis and toxin-production-related mcy gene.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Li D,Gu AZ,He Mdoi
10.1111/jam.12456subject
Has Abstractpub_date
2014-06-01 00:00:00pages
1482-94issue
6eissn
1364-5072issn
1365-2672journal_volume
116pub_type
杂志文章abstract:AIM:To develop a novel validated method for the isolation of Bifidobacterium animalis ssp. lactis BB-12 (BB-12) from faecal specimens and apply it to studies of BB-12 and Lactobacillus rhamnosus GG (LGG) recovered from the healthy human gastrointestinal (GI) tract. METHODS AND RESULTS:A novel method for isolating and ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,随机对照试验
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abstract::Ecological and physiological effects of the sulphate-reducing bacterium (SRB) Desulfovibrio desulfuricans on other intestinal organisms were investigated in anaerobic chemostats (dilution rate approximately 0.2 h-1). Reproducible defined bacterial communities were used in these experiments, comprising 14 different sac...
journal_title:Journal of applied microbiology
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更新日期:1998-08-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2003.02079.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:
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journal_title:Journal of applied microbiology
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更新日期:2015-10-01 00:00:00
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journal_title:Journal of applied microbiology
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abstract::Members of the genus Nocardiopsis are an ecologically versatile and biotechnologically important group of Actinomycetes. Most of the isolates are halotolerant or halophilic and they prevail in soils, marine environments or hypersaline locations. To aid their survival under these conditions, they mainly produce extremo...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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更新日期:2016-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04092.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03468.x
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03255.x
更新日期:2007-08-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02218.x
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journal_title:Journal of applied microbiology
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更新日期:2012-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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更新日期:2017-10-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2014-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03432.x
更新日期:2007-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-08-22 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00339.x
更新日期:1998-02-01 00:00:00
abstract:AIMS:Pseudomonas spp. are considered the most important milk spoilage organisms. Here we describe development of a fluorescence in situ hybridization (FISH) probe specific for detection and enumeration of Pseudomonas spp. in milk. METHODS AND RESULTS:16S rRNA sequences were analysed to develop specific oligonucleotide...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01930.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To develop an effective multiplex polymerase chain reaction (PCR) for the simultaneous detection of three important Vibrio species, Vibrio cholerae (Vc), V. parahaemolyticus (Vp) and V. vulnificus (Vv) using the groEL gene, a potential phylogenetic marker. METHODS AND RESULTS:Three species-specific primer sets we...
journal_title:Journal of applied microbiology
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更新日期:2013-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2021-01-22 00:00:00