Environment and virulence factors of Vibrio cholerae strains isolated in Argentina.

Abstract:

AIMS:To determine the presence of Vibrio cholerae in different areas of Argentina in three sample types, to determine the composition of planktonic communities in areas at which this pathogen was detected and to characterize the virulence properties and antimicrobial resistance of the recovered environmental isolates. METHODS AND RESULTS:Water and plankton samples were collected in marine, brackish and freshwater environments. Vibrio cholerae non-O1, non-O139 was isolated in 36.1% of the samples analysed. The micro-organism was detected in freshwater but not in marine or brackish samples. No relationship was found between isolation of V. cholerae and presence of any species of plankton. All the isolates presented very similar virulence profiles by PCR, lacking ctxA and tcpA El Tor and containing hlyA (98.7%), rtxA (99.0%), toxR (98.7%) and stn-sto (1.9%). Resistance to ampicillin was found in both Tucumán (21%) and Buenos Aires isolates (45%). CONCLUSIONS:We identified two geographic areas in Argentina where V. cholerae was present: freshwaters of the rivers from Tucumán and the Río de la Plata. SIGNIFICANCE AND IMPACT OF THE STUDY:The identification of V. cholerae strains in the environment, carrying both virulence factors and resistance to antimicrobial agents, highlight the need for a continuous and active surveillance of this pathogen.

journal_name

J Appl Microbiol

authors

Fraga SG,Pichel M,Costagliola M,Cecilia M,Jurquiza V,Peressutti S,Caffer MI,Aulet O,Hozbor C,Tracanna BC,de Gamundi AV,Hernández D,Ramírez FC,Akselman R,Binsztein N

doi

10.1111/j.1365-2672.2007.03468.x

subject

Has Abstract

pub_date

2007-12-01 00:00:00

pages

2448-56

issue

6

eissn

1364-5072

issn

1365-2672

pii

JAM3468

journal_volume

103

pub_type

杂志文章
  • The synergy of resveratrol and alcohol against Helicobacter pylori and underlying anti-Helicobacter pylori mechanism of resveratrol.

    abstract:AIMS:To determine individual antibacterial and synergistic antibacterial effects of resveratrol and alcohol against Helicobacter pylori 26695 in vitro, and to elucidate the underlying mechanism of action of resveratrol against H. pylori. METHODS AND RESULTS:The minimum inhibitory concentrations (MICs) and time-killing...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14531

    authors: Xia M,Chen H,Liu S

    更新日期:2020-04-01 00:00:00

  • Prior exposure of agriculture cephalosporin ceftiofur impaired conjugation of blaCTX-M-65 gene-bearing plasmid in Salmonella Saintpaul.

    abstract:AIMS:Although a link between agricultural cephalosporin use and resistance in Salmonella has been demonstrated with the drug ceftiofur, the underlying mechanism of the correlation is unclear. This study investigated the impact of ceftiofur exposure in S. Saintpaul on ceftriaxone resistance, the gene expression and the ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14749

    authors: Zwe YH,Yuk HG

    更新日期:2020-12-01 00:00:00

  • Arcobacter butzleri isolates exhibit pathogenic potential in intestinal epithelial cell models.

    abstract:AIMS:The pathogenic potential of Arcobacter butzleri isolates on human (HT-29/B6) and porcine epithelial (IPEC-J2) cells was investigated by in vitro assays. METHODS AND RESULTS:Five of six A. butzleri isolates were able to adhere and invade HT-29/B6 cells while only four isolates adhered and two invaded IPEC-J2 cells...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12979

    authors: Karadas G,Bücker R,Sharbati S,Schulzke JD,Alter T,Gölz G

    更新日期:2016-01-01 00:00:00

  • The influence of packaging methodology on the microbiological and fatty acid profiles of refrigerated African catfish fillets.

    abstract:AIMS:The shelf-life of refrigerated catfish fillets was determined at 2 degrees C, to simulate retail conditions, using two types of packaging materials, vacuum packing (VP) and oxygen permeable packaging (OPP). METHODS AND RESULTS:Representative samples (n=5) from both types of packaging methods were drawn at random ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01346.x

    authors: Anelich LE,Hoffman LC,Swanepoel MJ

    更新日期:2001-07-01 00:00:00

  • Non-Tuberculosis mycobacterium speciation using HPLC under Revised National TB Control Programme (RNTCP) in India.

    abstract:AIMS:Non-Tuberculous Mycobacteria (NTM) are ubiquitous in nature. The data on prevalence of NTM under the RNTCP is scarce. Many NTM species have clinical significance, and hence their identification and speciation are important. METHODS AND RESULTS:It is a cross-sectional study conducted at the five RNTCP accredited c...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13604

    authors: Sebastian G,Nagaraja SB,Vishwanatha T,Voderhobli M,Vijayalakshmi N,Kumar P

    更新日期:2018-01-01 00:00:00

  • Activities of muscadine grape skin and quercetin against Helicobacter pylori infection in mice.

    abstract:AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04870.x

    authors: Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

    更新日期:2011-01-01 00:00:00

  • Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts as influenced by compost type, storage condition and inoculum level.

    abstract:AIM:Survival of Escherichia coli O157:H7 and Salmonella enterica in animal waste-based composts was studied with different compost types, storage conditions and inoculum levels. METHODS AND RESULTS:A cocktail of three E. coli O157:H7 or S. enterica strains was inoculated into dairy manure-based composts (A and B) or p...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13719

    authors: Chen Z,Kim J,Jiang X

    更新日期:2018-05-01 00:00:00

  • Antimicrobial activity of hop extracts against foodborne pathogens for meat applications.

    abstract:AIMS:The objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against Listeria monocytogenes in a model meat marinade and on marinated pork tenderloins. METHODS AND RESULTS:In a first s...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12717

    authors: Kramer B,Thielmann J,Hickisch A,Muranyi P,Wunderlich J,Hauser C

    更新日期:2015-03-01 00:00:00

  • Antibiotic resistance in bacteria associated with coarse atmospheric particulate matter in an urban area.

    abstract:AIMS:To assess antibiotic resistance in airborne bacteria associated with coarse particulate matter (PM10) in an urban area, with specific considerations about the Staphylococcus genus. METHODS AND RESULTS:Disc diffusion test was performed on 243 microbial strains, isolated from PM10 in winter and summer and belonging...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05018.x

    authors: Gandolfi I,Franzetti A,Bertolini V,Gaspari E,Bestetti G

    更新日期:2011-06-01 00:00:00

  • Combination of antagonistic yeasts with two food additives for control of brown rot caused by Monilinia fructicola on sweet cherry fruit.

    abstract:AIMS:To evaluate beneficial effect of two food additives, ammonium molybdate (NH4-Mo) and sodium bicarbonate (NaBi), on antagonistic yeasts for control of brown rot caused by Monilinia fructicola in sweet cherry fruit under various storage conditions. The mechanisms of action by which food additives enhance the efficac...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02821.x

    authors: Qin GZ,Tian SP,Xu Y,Chan ZL,Li BQ

    更新日期:2006-03-01 00:00:00

  • The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017.

    abstract:AIMS:To optimize the nutritional and environmental conditions for growth of and poly-beta-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. METHODS AND RESULTS:An isolate, identified as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammoni...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01590.x

    authors: Borah B,Thakur PS,Nigam JN

    更新日期:2002-01-01 00:00:00

  • Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR.

    abstract:AIMS:The aim of this work was to evaluate the antiviral activities of Baccharis dracunculifolia (extract and essential oil), propolis and some isolated compounds (caffeic and cinnamic acids) against poliovirus type 1 (PV1) replication in HEp-2 cells. METHOD:Three different protocols (pre-, simultaneous and post-treatm...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04354.x

    authors: Búfalo MC,Figueiredo AS,de Sousa JP,Candeias JM,Bastos JK,Sforcin JM

    更新日期:2009-11-01 00:00:00

  • Effects of small increases in copper levels on culturable basidiomycetous yeasts in low-nutrient soils.

    abstract:AIMS:Investigating the effect of perturbations, with relatively low Cu concentrations, on yeast community composition in low-nutrient virgin soil. METHODS AND RESULTS:Culturable soil yeast populations were monitored at an experimental site treated with the fungicide copper oxychloride (10 mg Cu per kg soil). Yeast num...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04770.x

    authors: Vreulink JM,Stone W,Botha A

    更新日期:2010-10-01 00:00:00

  • Identification of a Brevibacterium marker gene specific to poultry litter and development of a quantitative PCR assay.

    abstract:AIM:To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. METHODS AND RESULTS:Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method ta...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04666.x

    authors: Weidhaas JL,Macbeth TW,Olsen RL,Sadowsky MJ,Norat D,Harwood VJ

    更新日期:2010-07-01 00:00:00

  • Recovery and chemical disinfection of foot-and-mouth disease and African swine fever viruses from porous concrete surfaces.

    abstract:AIMS:Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV) from porous concrete. Compare ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14694

    authors: Gabbert LR,Neilan JG,Rasmussen M

    更新日期:2020-11-01 00:00:00

  • Preparation of an inoculum of Gluconacetobacter xylinus without mutants in shaken culture.

    abstract:AIMS:A high-quality inoculum of Gluconacetobacter xylinus is important to produce bacterial cellulose (BC), a versatile biomaterial. This work aims to develop a method of preparing an inoculum of this bacterium with high cell density and without mutants. METHODS AND RESULTS:Inocula of G. xylinus ACCC 10220 without and...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13193

    authors: Wang ZG,Xiang D,Wang XB,Li CF

    更新日期:2016-09-01 00:00:00

  • Quantification and genetic diversity of total and microcystin-producing Microcystis during blooming season in Tai and Yang-cheng lakes, China.

    abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12456

    authors: Li D,Gu AZ,He M

    更新日期:2014-06-01 00:00:00

  • Proposal of a method for the genetic transformation of Gordonia jacobaea.

    abstract:AIMS:Gordonia jacobaea is a recently isolated bacterial species with potential industrial application on account of its ability to store large quantities of trans-canthaxanthin. Its genetic manipulation is, however, difficult and cumbersome owing to the presence of mycolic acids in the cell wall and, especially, becaus...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02806.x

    authors: Veiga-Crespo P,Feijoo-Siota L,de Miguel T,Poza M,Villa TG

    更新日期:2006-03-01 00:00:00

  • Effect of environmental and physiological factors on the antibacterial activity of Curvularia haloperoxidase system against Escherichia coli.

    abstract:AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02491.x

    authors: Hansen EH,Schäfer T,Molin S,Gram L

    更新日期:2005-01-01 00:00:00

  • Modelling the lag time and growth rate of Aspergillus section Nigri IOC 4573 in mango nectar as a function of temperature and pH.

    abstract:AIMS:To assess the behaviour of Aspergillus section Nigri IOC 4573 in mango nectar as affected by temperature and pH. METHODS AND RESULTS:A central composite design (2(2)+2*2+3) was used to estimate the influence of temperature (17·2-22·8°C) and pH (3·28-4·7) on A. section Nigri growth (λ, lag time; μ, growth rate). S...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04803.x

    authors: Silva AR,Sant'Ana AS,Massaguer PR

    更新日期:2010-09-01 00:00:00

  • Apple bagasse as a substrate for the propagation of Patagonian wine yeast biomass.

    abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14216

    authors: Bravo SME,Morales M,Del Mónaco SM,Caballero AC

    更新日期:2019-05-01 00:00:00

  • Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction.

    abstract::An environmental soil survey to detect Burkholderia pseudomallei was performed during the dry and wet seasons in Darwin, Northern Territory, Australia. Soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. Selective cultu...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:

    authors: Brook MD,Currie B,Desmarchelier PM

    更新日期:1997-05-01 00:00:00

  • Ready-to-eat vegetable salads served in Nigerian restaurants: a potential source of multidrug-resistant bacteria.

    abstract:AIM:Public health promotion of fruit and vegetables as healthier sources of nutrition in Nigeria has led to an increase in consumption of fruit and vegetables, particularly in the form of ready-to-eat (RTE) salads. However, the continual association of drug-resistant bacteria with fruit and vegetables creates an additi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14693

    authors: Okafor-Elenwo EJ,Imade OS

    更新日期:2020-11-01 00:00:00

  • In vitro effect of phenolic antioxidants on germination, growth and aflatoxin B accumulation by peanut Aspergillus section Flavi.

    abstract:AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was de...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02661.x

    authors: Passone MA,Resnik SL,Etcheverry MG

    更新日期:2005-01-01 00:00:00

  • Transcriptomic approach and membrane fatty acid analysis to study the response mechanisms of Escherichia coli to thyme essential oil, carvacrol, 2-(E)-hexanal and citral exposure.

    abstract:AIMS:The application of essential oils (EOs) and their components as food preservatives is promising but requires a deeper understanding of their mechanisms of action. This study aims to evaluate the effects of thyme EO, carvacrol, citral and 2-(E)-hexenal, on whole-genome gene expression (the transcriptome), as well a...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14048

    authors: Siroli L,Braschi G,de Jong A,Kok J,Patrignani F,Lanciotti R

    更新日期:2018-11-01 00:00:00

  • Taxonomic and functional diversity of atrazine-degrading bacterial communities enriched from agrochemical factory soil.

    abstract:AIMS:To characterize atrazine-degrading potential of bacterial communities enriched from agrochemical factory soil by analysing diversity and organization of catabolic genes. METHODS AND RESULTS:The bacterial communities enriched from three different sites of varying atrazine contamination mineralized 65-80% of (14) C...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04700.x

    authors: Udiković-Kolić N,Hršak D,Devers M,Klepac-Ceraj V,Petrić I,Martin-Laurent F

    更新日期:2010-07-01 00:00:00

  • Biological control of Pseudomonas syringae pv. aptata on sugar beet with Bacillus pumilus SS-10.7 and Bacillus amyloliquefaciens (SS-12.6 and SS-38.4) strains.

    abstract:AIM:Assessment of biological control of Pseudomonas syringae pv. aptata using crude lipopeptide extracts (CLEs) of two Bacillus amyloliquefaciens strains (SS-12.6 and SS-38.4) and one Bacillus pumilus strain (SS-10.7). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of CLEs and their combinations agains...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14070

    authors: Nikolić I,Berić T,Dimkić I,Popović T,Lozo J,Fira D,Stanković S

    更新日期:2019-01-01 00:00:00

  • Standard method for deposition of dry, aerosolized, silica-coated Bacillus spores onto inanimate surfaces.

    abstract:AIMS:To evaluate a standard aerosolization method for uniformly depositing threat-representative spores onto surfaces. METHODS AND RESULTS:Lyophilized Bacillus anthracis ΔSterne spores, coated in silica, were aerosolized into a containment chamber and deposited onto nine surface types by two independent laboratories. ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12509

    authors: Harnish D,Heimbuch BK,McDonald M,Kinney K,Dion M,Stote R,Rastogi V,Smith L,Wallace L,Lumley A,Schreuder-Gibson H,Wander JD

    更新日期:2014-07-01 00:00:00

  • Characterization of a fibronectin-binding protein from Lactobacillus casei BL23.

    abstract:AIMS:To characterize the functionality of the Lactobacillus casei BL23 fbpA gene encoding a putative fibronectin-binding protein. METHODS AND RESULTS:Adhesion tests showed that L. casei BL23 binds immobilized and soluble fibronectin in a protease-sensitive manner. A mutant with inactivated fbpA showed a decrease in bi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04508.x

    authors: Muñoz-Provencio D,Pérez-Martínez G,Monedero V

    更新日期:2010-03-01 00:00:00

  • Purification and characterization of a branched-chain amino acid aminotransferase from Lactobacillus paracasei subsp. paracasei CHCC 2115.

    abstract:AIM:Purification and characterization of an aminotransferase (AT) specific for the degradation of branched-chain amino acids from Lactobacillus paracasei subsp. paracasei CHCC 2115. METHODS AND RESULTS:The purification protocol consisted of anion exchange chromatography, affinity chromatography and hydrophobic interac...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02163.x

    authors: Thage BV,Rattray FP,Laustsen MW,Ardö Y,Barkholt V,Houlberg U

    更新日期:2004-01-01 00:00:00