Abstract:
AIM:To identify a DNA sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources. METHODS AND RESULTS:Faecally contaminated poultry litter and soils were used as source material for the development of a quantitative polymerase chain reaction (qPCR) method targeting the 16S rRNA gene of a Brevibacterium sp. The identified sequence had 98% nucleotide identity to the 16S rRNA gene of Brevibacterium avium. The qPCR method was tested on 17 soiled litter samples; 40 chicken faecal samples; and 116 nontarget faecal samples from cattle, swine, ducks, geese, and human sewage collected across the United States. The 571-bp product was detected in 76% of poultry-associated samples, but not in 93% of faecal samples from other sources. Marker concentrations were 10(7) -10(9) gene copies per gram in soiled litter, up to 10(5) gene copies per gram in spread-site soils, and 10(7) gene copies per litre in field run-off water. Results were corroborated by a blinded study conducted by a second laboratory. CONCLUSION:The poultry-specific PCR product is a useful marker gene for assessing the impact of faecal contamination as a result of land-applied poultry litter. SIGNIFICANCE AND IMPACT OF THE STUDY:This study describes the first quantitative, sensitive and specific microbial source tracking method for the detection of poultry litter contamination.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Weidhaas JL,Macbeth TW,Olsen RL,Sadowsky MJ,Norat D,Harwood VJdoi
10.1111/j.1365-2672.2010.04666.xsubject
Has Abstractpub_date
2010-07-01 00:00:00pages
334-47issue
1eissn
1364-5072issn
1365-2672pii
JAM4666journal_volume
109pub_type
杂志文章abstract:AIMS:To investigate the effect of media composition and agroindustrial residues on bovicin HC5 production by Streptococcus bovis HC5. METHODS AND RESULTS:Batch cultures of S. bovis HC5 were grown in basal medium containing different carbon and nitrogen sources. The activity of cell-free and cell-associated bovicin HC5...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2009.04212.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:To characterize the non-spoilage variant obtained from beer-spoilage Lactobacillus brevis ABBC45C and to identify a potential genetic marker capable of discriminating beer-spoilage L. brevis strains from non-spoilers. METHODS AND RESULTS:A non-spoilage variant was obtained from beer-spoilage L. brevis ABBC45C by ...
journal_title:Journal of applied microbiology
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abstract:AIMS:To determine the activity of enterocin AS-48 against ropy-forming Bacillus licheniformis from cider. METHODS AND RESULTS:Enterocin AS-48 was tested on B. licheniformis LMG 19409 from ropy cider in MRS-G broth, fresh-made apple juice and in two commercial apple ciders (A and B). Bacillus licheniformis was rapidly ...
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abstract:AIMS:To study Bacillus clausii from a pharmaceutical product (Enterogermina O/C, N/R, SIN, T) and reference strains (B. clausii and Bacillus subtilis) for eco-physiological aspects regarding the gut environment. METHODS AND RESULTS:Spores and vegetative cells were challenged in vitro miming the injury of gastrointesti...
journal_title:Journal of applied microbiology
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abstract:AIMS:The aim of this study was to evaluate the persistence of Pseudomonas fluorescens LBUM677 in the rhizosphere of Buglossoides arvensis under agricultural field conditions and determine if B. arvensis intraspecific genetic variations affect the capacity of LBUM677 to colonize its rhizosphere and increase seed oil and...
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abstract:AIMS:Bacillus cereus sensu lato is a complex group of closely related bacteria, which are commonly present in the natural environment and food products. These organisms may cause food poisoning and spoilage as well as opportunistic infections. Thus far, their resistance to selected antibiotics has been explored only in...
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更新日期:2002-01-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.1997.00312.x
更新日期:1998-01-01 00:00:00
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更新日期:2017-12-01 00:00:00
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更新日期:2017-03-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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更新日期:2004-01-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.01021.x
更新日期:2000-04-01 00:00:00
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更新日期:2016-12-01 00:00:00