Abstract：
AIMS:To investigate the effect of diet on the survival of Salmonella in the bovine abomasum. METHODS AND RESULTS:Five fistulated cows were randomly assigned to one of five diets denoted as: (i) 100% grass, (ii) grass + 5·3 kg DM concentrate, (iii) 100% grass silage, (iv) 100% hay and (v) maize/grass silage plus concentrates. Rumen fluid was harvested from each dietary treatment and inoculated with nonacid (NA) and acid-adapted (AA) 5-strain Salmonella cocktails. After 24-h incubation period, Salmonella were acid challenged to synthetic abomasum fluid (SAF, pH 2·5) for 5 h to determine their resistance to low pH. The study found that the volatile fatty acids composition and the pH profile of bovine rumen fluid were significantly altered (P <0·05) by some of the dietary treatments but not others. Regression analysis found that significantly higher numbers of acid-adapted Salmonella survived in SAF after incubation in rumen fluid from diets 1, 2 and 4, but fewer significant differences were found between diets for nonacid-adapted Salmonella. The results suggest that the acid-adapted cells were subjected to a higher level of cell injury than the nonadapted cells. CONCLUSIONS: Pre-incubation in rumen fluid did influence the resistance of nonacid and acid-adapted Salmonella to SAF but it was dependant on the dietary treatment fed to the cows. SIGNIFICANCE AND IMPACT OF THE STUDY: This study examined the use of diet, as a modulating factor to limit the bovine excretion of Salmonella with a view to providing a scientific basis for the design of dietary management controls in the future.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Lenahan M,Kelly S,Fanning S,Bolton DJdoi
10.1111/j.1365-2672.2010.04836.xsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
2060-8issue
6eissn
1364-5072issn
1365-2672pii
JAM4836journal_volume
109pub_type
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