Abstract:
AIMS:To investigate the changes in the surface properties of Lactobacillus rhamnosus GG during growth, and relate them with the ability of the Lactobacillus cells to adhere to Caco-2 cells. METHODS AND RESULTS:Lactobacillus rhamnosus GG was grown in complex medium, and cell samples taken at four time points and freeze dried. Untreated and trypsin treated freeze dried samples were analysed for their composition using SDS-PAGE analysis and Fourier transform infrared spectroscopy (FTIR), hydrophobicity and zeta potential, and for their ability to adhere to Caco-2 cells. The results suggested that in the case of early exponential phase samples (4 and 8 h), the net surface properties, i.e. hydrophobicity and charge, were determined to a large extent by anionic hydrophilic components, whereas in the case of stationary phase samples (13 and 26 h), hydrophobic proteins seemed to play the biggest role. Considerable differences were also observed between the ability of the different samples to adhere to Caco-2 cells; maximum adhesion was observed for the early stationary phase sample (13 h). The results suggested that the adhesion to Caco-2 cells was influenced by both proteins and non-proteinaceous compounds present on the surface of the Lactobacillus cells. CONCLUSION:The surface properties of Lact. rhamnosus GG changed during growth, which in return affected the ability of the Lactobacillus cells to adhere to Caco-2 cells. SIGNIFICANCE AND IMPACT OF THE STUDY:The levels of adhesion of Lactobacillus cells to Caco-2 cells were influenced by the growth time and reflected changes on the bacterial surface. This study provides critical information on the physicochemical factors that influence bacterial adhesion to intestinal cells.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Deepika G,Green RJ,Frazier RA,Charalampopoulos Ddoi
10.1111/j.1365-2672.2009.04306.xsubject
Has Abstractpub_date
2009-10-01 00:00:00pages
1230-40issue
4eissn
1364-5072issn
1365-2672pii
JAM4306journal_volume
107pub_type
杂志文章abstract::The effects of steroidal saponins (SAP) isolated from Yucca schidigera extract on ruminal bacteria and fungi were investigated in pure culture studies. Prevotella bryantii, Ruminobacter amylophilus, Selenomonas ruminantium and Streptococcus bovis were cultured through ten 24-h transfers in ruminal fluid medium contain...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01054.x
更新日期:2000-05-01 00:00:00
abstract:AIMS: To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. METHODS AND RESULTS: The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluor...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.04964.x
更新日期:2011-04-01 00:00:00
abstract:AIMS:To identify proteins regulating antimicrobial peptide (AMP) resistance in Vibrio parahaemolyticus using membrane subproteome analysis. METHODS AND RESULTS:Three synthetic AMPs (Q4, Q6 and H1) and a natural one from fish (pleurocidin) were used for selection of AMP-resistant strains. Differential expression patter...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04544.x
更新日期:2010-04-01 00:00:00
abstract:AIMS:The aim of this study was to examine the contribution of a five-gene islet (lmo0444 - lmo0448) to the growth of Listeria monocytogenes under suboptimal conditions. METHODS AND RESULTS:Bioinformatics and PCR analyses revealed that a five-gene islet is present in c. half of all L. monocytogenes strains examined (66...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04726.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:Root rot caused by a group of fungi is a serious disease in mulberry. This study aims to identify and characterize Rhizopus oryzae and other fungal species associated with root rot of mulberry in India. METHODS AND RESULTS:Rotted root samples were collected from the mulberry gardens from four states of Southern I...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14959
更新日期:2020-12-04 00:00:00
abstract:AIMS:To develop sensitive quantitative PCR assays for the two groups of pathogens responsible for Fusarium seedling blight in wheat: Fusarium group (Fusarium culmorum and Fusarium graminearum) and Microdochium group (Microdochium nivale and Microdochium majus); and to use the assays to assess performance of fungicide s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03190.x
更新日期:2007-06-01 00:00:00
abstract:AIMS:The isolation of lytic bacteriophage of Vibrio harveyi with potential for phage therapy of bacterial pathogens of phyllosoma larvae from the tropical rock lobster Panulirus ornatus. METHODS AND RESULTS:Water samples from discharge channels and grow-out ponds of a prawn farm in northeastern Australia were enriched...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04578.x
更新日期:2010-05-01 00:00:00
abstract:AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02385.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To determine whether isolates of Listeria monocytogenes differ in their ability to adsorb and form biofilms on a food-grade stainless steel surface. METHODS AND RESULTS:Strains were assessed for their ability to adsorb to a test surface over a short time period. Although some differences in numbers of bound cells...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01419.x
更新日期:2001-10-01 00:00:00
abstract:AIMS:The purpose of this study was to compare the effects of feeding virginiamycin or bacitracin methylene disalicylate (BMD), two in-feed antibiotics typically used by commercial poultry producers in the United States, on the chicken gastrointestinal microbiota. METHODS AND RESULTS:454 pyrosequencing of the V6-V8 reg...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12960
更新日期:2015-12-01 00:00:00
abstract:AIMS:Lactococcus lactis N8 and Saccharomyces boulardii SAA655 were investigated for their ability to synthesize B-vitamins (riboflavin and folate) and their functional role as microbial starters in idli fermentation. METHODS AND RESULTS:In this study, ultra-high performance liquid chromatography and microbiological as...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13453
更新日期:2017-06-01 00:00:00
abstract:AIMS:To determine the ability of a bismuth thiol to control floc formation in a multispecies population of micro-organisms obtained from the activated sludge unit of a wastewater treatment plant. The molecular level mechanisms by which bismuth-2-3-dimercapto-1-propanol (BisBAL) inhibits bioaggregation are also elucidat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05002.x
更新日期:2011-06-01 00:00:00
abstract::An alkalophilic, environmental micro-organism, Bacillus sp. BG-11, has been isolated and characterized. It produced 76 U ml-1 of chitinase in liquid batch fermentation after 72 h of incubation at 50 degrees C using chitin-enriched medium. The molecular weight of purified chitinase was estimated to be 41 kDa by SDS-PAG...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01016.x
更新日期:2000-05-01 00:00:00
abstract:AIMS:To investigate bacterial communities of tyre monofill sites, colonization of tyre material by bacteria and the effect of tyre leachate on bacteria. METHODS AND RESULTS:Culturable bacteria were isolated from buried tyre shreds and identified using fatty acid methyl ester analysis. Isolates belonged to taxonomic gr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04157.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12456
更新日期:2014-06-01 00:00:00
abstract::With the emergence of antibiotic resistance, the interest for antimicrobial agents has recently increased again in public health. Copper was recognized in 2008 by the United States Environmental Protection Agency (EPA) as the first metallic antimicrobial agent. This led to many investigations of the various properties...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13681
更新日期:2018-05-01 00:00:00
abstract::Biocides (antiseptics and disinfectants) are widely used in hospitals and pharmaceutical industries for contamination control. The emergence of reduced susceptibility to biocides is the major concern and this is caused by various factors, among which plasmid-mediated resistance is common. Many publications describe th...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14118
更新日期:2019-04-01 00:00:00
abstract:AIMS:Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS:B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03847.x
更新日期:2008-10-01 00:00:00
abstract:AIMS:To determine the prevalence of enterotoxin-producing Staphylococcus intermedius in dogs and pigeons. METHODS AND RESULTS:A total of 106 S. intermedius isolates from 44 dogs and 62 pigeons were tested for the production of enterotoxins A, B, C and D by reverse passive latex agglutination (RPLA) and for sec-canine ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02264.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To enhance the recovery of f(2) bacteriophage and poliovirus by an established method based on the adsorption to and elution from positively-charged Al(OH)(3)-treated silica gel. METHODS AND RESULTS:Polyaluminum Chloride (PAC) was added to water samples to neutralize the negatively charged materials, which can re...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04041.x
更新日期:2009-02-01 00:00:00
abstract:AIM:Introduction of a protocol for broad-range diagnosis of bacterial infections, which remain negative in culture. METHODS AND RESULTS:The new TaqMan real-time PCR assay amplifies part of the 16S rRNA gene. Species are identified by subsequent sequencing and phylogenetic blast analysis. The analytical sensitivity sho...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03648.x
更新日期:2008-04-01 00:00:00
abstract::The regrowth of micro-organisms in the water distribution system is assisted by the presence of biodegradable organic matter (BDOC). The low concentration of available organic carbon in this type of water favours the growth of bacteria belonging to the Pseudomonadaceae family, as this group can grow better at low conc...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01021.x
更新日期:2000-04-01 00:00:00
abstract:AIMS:This study was performed to investigate the effects of Megasphaera elsdenii (Me), Saccharomyces cerevisiae (SC) and lactic acid bacteria (FP - Lactobacillus fermentum plus Lactobacillus plantarum) alone or in combination on biogas production and ruminal biofermentation parameter in a heterofermenter system. METHO...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14990
更新日期:2021-01-07 00:00:00
abstract:AIMS:To identify native Antarctic bacteria capable of oil degradation at low temperatures. METHODS AND RESULTS:Oil contaminated and pristine soils from Signy Island (South Orkney Islands, Antarctica) were examined for bacteria capable of oil degradation at low temperatures. Of the 300 isolates cultured, Pseudomonas st...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02678.x
更新日期:2005-01-01 00:00:00
abstract::During the first half of the century, the majority of anthrax outbreaks in Canada occurred in the southern portions of Ontario and Quebec and were often associated with pastures contaminated by effluent from textile industries dealing with imported animal materials. In 1952, introduction of Federal regulations requiri...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1046/j.1365-2672.1999.00872.x
更新日期:1999-08-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12351
更新日期:2014-01-01 00:00:00
abstract:AIMS:To validate the use of the air pouch system to predict and examine early immune responses induced by the presumptive probiotics Lactobacillus paracasei subsp. paracasei B112, DC205, DC215 and DC412 strains in the gut mucosa. METHODS AND RESULTS:Only the DC412 strain interacted strongly with the cells forming the ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04421.x
更新日期:2010-01-01 00:00:00
abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14216
更新日期:2019-05-01 00:00:00
abstract::The effect of different levels of salt, sodium nitrite, polyphosphate and various sugars on growth, pigment production, protease activity and culture pH caused by Monascus purpureus was studied in broth medium and ground meat. The addition of sodium chloride (> 50.0 g l(-1)) and polyphosphate (> 3.0g l(-1)) to broth m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00821.x
更新日期:2000-01-01 00:00:00
abstract:AIMS:To compare clinical and environmental Aspergillus fumigatus strains on their toxicogenic and immunosuppressive capacity. METHODS AND RESULTS:A total of 51 strains of A. fumigatus isolated from clinical and corn silage samples were assayed. All A. fumigatus strains were assayed for gliotoxin production, therefore ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12673
更新日期:2015-01-01 00:00:00