Comparison of toxicogenic and immunosuppressive capacity of Aspergillus fumigatus strains isolated from clinical and corn silage samples.

Abstract:

AIMS:To compare clinical and environmental Aspergillus fumigatus strains on their toxicogenic and immunosuppressive capacity. METHODS AND RESULTS:A total of 51 strains of A. fumigatus isolated from clinical and corn silage samples were assayed. All A. fumigatus strains were assayed for gliotoxin production, therefore strains with different gliotoxin capacities and isolated from different sources were selected and assayed for their effects on bovine macrophages and lymphocytes. Spore diffusates (SDs) obtained from all A. fumigatus strains were able to inhibit macrophage phagocytosys, regardless of their gliotoxin production capacity. However, most but not all strains were able to inhibit bactericidal activity. SDs from all A. fumigatus strains reduced lymphocytes viability. The heat treatment was not always able to inhibit the negative effect on immune cells. CONCLUSIONS:There was no difference between clinical and environmental isolates in their toxicogenic and immunosuppressive capacity. Gliotoxin would not be responsible for the immunosuppressive activity observed by the assayed A. fumigatus strains. However, gliotoxin could be present in the SD, together with some other substances. SIGNIFICANCE AND IMPACT OF THE STUDY:The results obtained suggest that any environmental strain of A. fumigatus is a putative infectious strain. Prevention measures should be applied to control environmental Aspergillus conidia.

journal_name

J Appl Microbiol

authors

Dogi C,Alonso V,Fochesato A,Poloni V,Cavaglieri L

doi

10.1111/jam.12673

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

175-81

issue

1

eissn

1364-5072

issn

1365-2672

journal_volume

118

pub_type

杂志文章
  • Development of multiplex real-time PCR for simultaneous detection of three Potyviruses in tobacco plants.

    abstract:AIMS:To develop a multiplex real-time PCR assay using TaqMan probes for the simultaneous detection and quantification of Tobacco etch virus (TEV), Potato virus Y (PVY) and Tobacco vein banding mosaic virus (TVBMV). METHODS AND RESULTS:Specific primer and probe combinations for TEV and TVBMV were developed from the coa...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12071

    authors: Dai J,Peng H,Chen W,Cheng J,Wu Y

    更新日期:2013-02-01 00:00:00

  • Rapid quantification of viable legionellae in water and biofilm using ethidium monoazide coupled with real-time quantitative PCR.

    abstract:AIMS:To optimize ethidium monoazide (EMA) coupled with real-time quantitative PCR (qPCR) and to evaluate its environmental applicability on quantifying viable legionellae in water and biofilm of cooling towers and hot water systems. METHODS AND RESULTS:EMA (0.9-45.5 microg ml(-1)) and propidium monoazide (PMA, 0.9 and...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04678.x

    authors: Chen NT,Chang CW

    更新日期:2010-08-01 00:00:00

  • Aflatoxin contamination of groundnut and maize in Zambia: observed and potential concentrations.

    abstract:AIMS:The aims of the study were to quantify aflatoxins, the potent carcinogens associated with stunting and immune suppression, in maize and groundnut across Zambia's three agroecologies and to determine the vulnerability to aflatoxin increases after purchase. METHODS AND RESULTS:Aflatoxin concentrations were determin...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13448

    authors: Kachapulula PW,Akello J,Bandyopadhyay R,Cotty PJ

    更新日期:2017-06-01 00:00:00

  • A metagenomic study of the preventive effect of Lactobacillus rhamnosus GG on intestinal polyp formation in ApcMin/+ mice.

    abstract:AIMS:To investigate the in vivo effects of Lactobacillus rhamnosus GG (LGG) on intestinal polyp development and the interaction between this single-organism probiotic and the gut microbiota therein. METHODS AND RESULTS:The ApcMin/+ mouse model was used to study the potential preventive effect of LGG on intestinal poly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13386

    authors: Ni Y,Wong VH,Tai WC,Li J,Wong WY,Lee MM,Fong FL,El-Nezami H,Panagiotou G

    更新日期:2017-03-01 00:00:00

  • Sampling and inactivation of wet disseminated spores from flooring materials, using commercially available robotic vacuum cleaners.

    abstract:AIMS:Four commercially available robotic vacuum cleaners were assessed for sampling efficiency of wet disseminated Bacillus atrophaeus spores on carpet, polyvinyl chloride (PVC) and laminate flooring. Furthermore, their operability was evaluated and decontamination efficiency of one robot was assessed, using a sodium h...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13910

    authors: Thompson KA,Paton S,Pottage T,Bennett AM

    更新日期:2018-10-01 00:00:00

  • Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci.

    abstract:AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03018.x

    authors: Perry JD,James AL,Morris KA,Oliver M,Chilvers KF,Reed RH,Gould FK

    更新日期:2006-11-01 00:00:00

  • Evaluation of micro-organism-detaching efficacy from meat samples by spindle or stomacher treatment and quality analysis of suspensions.

    abstract:AIMS:We investigated and compared the efficacy of a new apparatus for detaching micro-organisms from meat samples. METHODS AND RESULTS:The efficacy of Spindle and stomacher in detaching micro-organisms from meat samples was evaluated. Also, evaluation of appropriateness of suspensions generated by both methods for car...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13075

    authors: Kim SJ,Kim DK,Kang DH

    更新日期:2016-04-01 00:00:00

  • A Streptococcus uberis transposon mutant screen reveals a negative role for LiaR homologue in biofilm formation.

    abstract:AIMS:The environmental pathogen Streptococcus uberis causes intramammary infections in dairy cows. Because biofilm growth might contribute to Strep. uberis mastitis, we conducted a biological screen to identify genes potentially involved in the regulation of biofilm growth. METHODS AND RESULTS:By screening a transposo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12664

    authors: Salomäki T,Karonen T,Siljamäki P,Savijoki K,Nyman TA,Varmanen P,Iivanainen A

    更新日期:2015-01-01 00:00:00

  • In vitro potential antioxidant activity of indigenous yeasts isolated from virgin olive oil.

    abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14520

    authors: Ciafardini G,Zullo BA

    更新日期:2020-03-01 00:00:00

  • Generation of bioaerosols during manual mail unpacking and sorting.

    abstract:AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02700.x

    authors: Brandl H,Bachofen R,Bischoff M

    更新日期:2005-01-01 00:00:00

  • Ochratoxin A removal in synthetic and natural grape juices by selected oenological Saccharomyces strains.

    abstract:AIMS:To assess, for the first time the efficiency in removing ochratoxin A (OTA) from laboratory medium [yeast peptone glucose (YPG)], synthetic grape juice medium (SGM) and natural grape juice by viable and dead (heat and acid-treated) oenological Saccharomyces strains (five S. cerevisiae and one S. bayanus) compared ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02385.x

    authors: Bejaoui H,Mathieu F,Taillandier P,Lebrihi A

    更新日期:2004-01-01 00:00:00

  • Molecular detection and genotyping of human noroviruses in influent and effluent water at a wastewater treatment plant in Japan.

    abstract:AIMS:To investigate the prevalence, seasonality and genotype distribution of human noroviruses (NoVs) in wastewater in Japan. METHODS AND RESULTS:Influent and effluent water samples were collected monthly for a year from a wastewater treatment plant and examined for the presence of genogroups I and II (GI and GII) NoV...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05231.x

    authors: Kitajima M,Haramoto E,Phanuwan C,Katayama H,Furumai H

    更新日期:2012-03-01 00:00:00

  • Assessment of bismuth thiols and conventional disinfectants on drinking water biofilms.

    abstract:AIMS:Biofilms in water distribution systems represent a far more significant reservoir of micro-organisms than the water phase. Biofilms are (i) resistant to disinfectants, (ii) nuclei for microbial regrowth, (iii) a refuge for pathogens, (iv) accompanied by taste and odour problems, and (v) corrode surfaces. The effec...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01974.x

    authors: Codony F,Domenico P,Mas J

    更新日期:2003-01-01 00:00:00

  • The survival of filoviruses in liquids, on solid substrates and in a dynamic aerosol.

    abstract:AIMS:Filoviruses are associated with high morbidity and lethality rates in humans, are capable of human-to-human transmission, via infected material such as blood, and are believed to have low infectious doses for humans. Filoviruses are able to infect via the respiratory route and are lethal at very low doses in exper...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04778.x

    authors: Piercy TJ,Smither SJ,Steward JA,Eastaugh L,Lever MS

    更新日期:2010-11-01 00:00:00

  • Conjugal transfer of plasmids and antibiotic resistance among Escherichia coli isolated from animals in a rural area in Sarawak (Malaysia).

    abstract::Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes fr...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:

    authors: Son R,Rusu G,Karim MI

    更新日期:1997-02-01 00:00:00

  • Comparison of the sensitivity of manual and automated immunomagnetic separation methods for detection of Shiga toxin-producing Escherichia coli O157:H7 in milk.

    abstract:AIM:To determine the sensitivity of methods for detection of injured and uninjured Escherichia coli O157:H7 (E. coli O157) in raw and pasteurized milk. METHODS AND RESULTS:Raw milk, pasteurized milk with 1.5% fat content and pasteurized milk with 3.5% fat content were spiked with E. coli O157 at low levels. The sample...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01646.x

    authors: Reinders RD,Barna A,Lipman LJ,Bijker PG

    更新日期:2002-01-01 00:00:00

  • In vitro evaluation of metal chelators as potential metallo- β -lactamase inhibitors.

    abstract:AIMS:This study aimed at investigating the use of metal chelators as potential metallo-β-lactamase inhibitors (MBL). METHODS AND RESULTS:The minimum inhibitory concentration (MIC) of meropenem was ascertained alone and in combination with various concentrations of macrocyclic (1,4,7- triazacyclononane-1-glutaric acid-...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13085

    authors: Azumah R,Dutta J,Somboro AM,Ramtahal M,Chonco L,Parboosing R,Bester LA,Kruger HG,Naicker T,Essack SY,Govender T

    更新日期:2016-04-01 00:00:00

  • Elevation of Francisella philomiragia subsp. noatunensis Mikalsen et al. (2007) to Francisella noatunensis comb. nov. [syn. Francisella piscicida Ottem et al. (2008) syn. nov.] and characterization of Francisella noatunensis subsp. orientalis subsp. nov.,

    abstract:AIMS:This study was conducted to clarify the taxonomic status of Francisella sp. strain Ehime-1, a fish pathogen, in relation to the fish pathogens F. piscicida and F. philomiragia subsp. noatunensis and to F. philomiragia subsp. philomiragia. METHODS AND RESULTS:Francisella sp. Ehime-1 was compared to F. piscicida, F...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04092.x

    authors: Ottem KF,Nylund A,Karlsbakk E,Friis-Møller A,Kamaishi T

    更新日期:2009-04-01 00:00:00

  • Vertical profiles of community and activity of methanotrophs in landfill cover soils of different age.

    abstract:AIM:Aerobic CH4 oxidation is an important process controlling CH4 release from landfills to the atmosphere. The aim of this study was to investigate the link between CH4 oxidation activity and methanotrophs abundance and diversity in landfill cover soils of different age. METHODS AND RESULTS:Among the three investigat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12263

    authors: Kong JY,Su Y,Zhang QQ,Bai Y,Xia FF,Fang CR,He R

    更新日期:2013-09-01 00:00:00

  • Identification and characterization of the two-component cell lysis cassette encoded by temperate bacteriophage phiPYB5 of Lactobacillus fermentum.

    abstract:AIMS:To characterize the two-component cell lysis cassette comprised of holin (Hyb5) and endolysin (Lyb5) encoded by Lactobacillus fermentum temperate bacteriophage phiPYB5, and illustrate the potential application of Lyb5 as therapeutic agents. METHODS AND RESULTS:The hyb5-lyb5 cassette was cloned from the genome lib...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03953.x

    authors: Wang S,Kong J,Zhang X

    更新日期:2008-12-01 00:00:00

  • Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis.

    abstract::Microbial succession during a laboratory-scale composting process of garbage was analysed by denaturing gradient gel electrophoresis (DGGE) combined with measurement of physicochemical parameters such as temperature, pH, organic acids, total dissolved organic carbon and water-soluble humic substance. From the temperat...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01177.x

    authors: Ishii K,Fukui M,Takii S

    更新日期:2000-11-01 00:00:00

  • DNA extraction method influences human milk bacterial profiles.

    abstract:AIMS:To evaluate four DNA extraction methods to elucidate the most effective method for bacterial DNA recovery from human milk (HM). METHODS AND RESULTS:Human milk DNA was extracted using the following methods: (i) Qiagen MagAttract Microbial DNA Isolation Kit (kit QM), (ii) Norgen Milk Bacterial DNA Isolation Kit (ki...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14780

    authors: Cheema AS,Stinson LF,Lai CT,Geddes DT,Payne MS

    更新日期:2021-01-01 00:00:00

  • Phages of enteric bacteria in fresh water with different levels of faecal pollution.

    abstract::Levels of somatic and F-specific coliphages, and phages infecting Bacteroides fragilis were measured in 257 samples collected in different freshwater environments with different levels and characteristics of faecal pollution. In samples with recent pollution of domestic origin, the numbers of the three groups of phage...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1997.00354.x

    authors: Araujo RM,Puig A,Lasobras J,Lucena F,Jofre J

    更新日期:1997-03-01 00:00:00

  • Adaptive changes in geranylgeranyl pyrophosphate synthase gene expression level under ethanol stress conditions in Oenococcus oeni.

    abstract:AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12351

    authors: Cafaro C,Bonomo MG,Salzano G

    更新日期:2014-01-01 00:00:00

  • The role of tissue type, sampling and nucleic acid purification methodology on the inferred composition of Pacific oyster (Crassostrea gigas) microbiome.

    abstract:AIMS:This study evaluated methods to sample and extract nucleic acids from Pacific oysters to accurately determine the microbiome associated with different tissues. METHODS AND RESULTS:Samples were collected from haemolymph, gill, gut and adductor muscle, using swabs and homogenates of solid tissues. Nucleic acids wer...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14326

    authors: Pathirana E,McPherson A,Whittington R,Hick P

    更新日期:2019-08-01 00:00:00

  • Vaginal microbial communities from synchronized heifers and cows with reproductive disorders.

    abstract:AIM:To evaluate changes in the resident microbial population in the cranial vaginal mucosa induced by a progesterone-releasing intravaginal device (PRID) compared to the vaginal microbiota of cows with reproductive disorders. METHODS AND RESULTS:Vaginal discharge was evaluated by clinical examination and a Vaginitis D...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13239

    authors: Gonzalez Moreno C,Fontana C,Cocconcelli PS,Callegari ML,Otero MC

    更新日期:2016-11-01 00:00:00

  • Anti-Listeria bacteriocin-producing bacteria from raw ewe's milk in northern Greece.

    abstract:AIMS:The isolation and partial characterization of anti-Listeria bacteriocin producing strains present in milk from areas of northern Greece in view of their potential use as protective cultures in food fermentations. METHODS AND RESULTS:Three hundred and thirty-two isolates were obtained from milk samples intended fo...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04932.x

    authors: Chanos P,Williams DR

    更新日期:2011-03-01 00:00:00

  • Shoe soles as a potential vector for pathogen transmission: a systematic review.

    abstract::Shoe soles are possible vectors for infectious diseases. Although studies have been performed to assess the prevalence of infectious pathogens on shoe soles and decontamination techniques, no systematic review has ever occurred. The aim of this study was to perform a systematic review of the literature to determine th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13250

    authors: Rashid T,VonVille HM,Hasan I,Garey KW

    更新日期:2016-11-01 00:00:00

  • UV-B radiation reduces in vitro germination of Metarhizium anisopliae s.l. but does not affect virulence in fungus-treated Aedes aegypti adults and development on dead mosquitoes.

    abstract:AIMS:Control of diurnal Aedes aegypti with mycoinsecticides should consider the exposure of fungus-treated adults to sunlight, and especially to UV-B radiation that might affect activity of conidia applied on the mosquito's surface. METHODS AND RESULTS:Germination of Metarhizium anisopliae s.l. IP 46 conidia on SDAY m...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13309

    authors: Falvo ML,Pereira-Junior RA,Rodrigues J,López Lastra CC,García JJ,Fernandes ÉK,Luz C

    更新日期:2016-12-01 00:00:00

  • Amoebae in domestic water systems: resistance to disinfection treatments and implication in Legionella persistence.

    abstract:AIMS:Monitoring of microbial changes during and after application of various disinfection treatments in a model domestic water system. METHODS AND RESULTS:A pilot-scale domestic water system consisting of seven galvanized steel re-circulation loops and copper dead legs was constructed. Culture techniques, confocal las...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02391.x

    authors: Thomas V,Bouchez T,Nicolas V,Robert S,Loret JF,Lévi Y

    更新日期:2004-01-01 00:00:00