Evaluation of novel fluorogenic substrates for the detection of glycosidases in Escherichia coli and enterococci.

Abstract:

AIMS:Enzyme substrates based on 4-methylumbelliferone are widely used for the detection of Escherichia coli and enterococci in water, by detection of beta-glucuronidase and beta-glucosidase activity respectively. This study aimed to synthesize and evaluate novel umbelliferone-based substrates with improved sensitivity for these two enzymes. METHODS AND RESULTS:A novel beta-glucuronide derivative based on 6-chloro-4-methylumbelliferone (CMUG) was synthesized and compared with 4-methylumbelliferyl-beta-D-glucuronide (MUG) using 42 strains of E. coli in a modified membrane lauryl sulfate broth. Over 7 h of incubation, the fluorescence generated from the hydrolysis of CMUG by E. coli was over twice that from MUG, and all of the 38 glucuronidase-positive strains generated a higher fluorescence with CMUG compared with MUG. Neither substrate caused inhibition of bacterial growth in any of the tested strains. Four beta-glucosidase substrates were also synthesized and evaluated in comparison with 4-methylumbelliferyl-beta-D-glucoside (MU-GLU) using 42 strains of enterococci in glucose azide broth. The four substrates comprised beta-glucoside derivatives of umbelliferone-3-carboxylic acid and its methyl, ethyl and benzyl esters. Glucosides of the methyl, ethyl and benzyl esters of umbelliferone-3-carboxylic acid, were found to be superior to MU-GLU for the detection of enterococci, especially after 18 h of incubation, while umbelliferone-3-carboxylic acid-beta-D-glucoside was inferior. However, the variability in detectable beta-glucosidase activity among the different strains of enterococci in short-term assays using the three carboxylate esters (7 h incubation) may compromise their use for rapid detection and enumeration of these faecal indicator bacteria. CONCLUSIONS:The beta-glucuronidase substrate CMUG appears to be a more promising detection system than the various beta-glucosidase substrates tested. SIGNIFICANCE AND IMPACT OF THE STUDY:The novel substrate CMUG showed enhanced sensitivity for the detection of beta-glucuronidase-producing bacteria such as E. coli, with a clear potential for application in rapid assays for the detection of this indicator organism in natural water and other environmental samples.

journal_name

J Appl Microbiol

authors

Perry JD,James AL,Morris KA,Oliver M,Chilvers KF,Reed RH,Gould FK

doi

10.1111/j.1365-2672.2006.03018.x

subject

Has Abstract

pub_date

2006-11-01 00:00:00

pages

977-85

issue

5

eissn

1364-5072

issn

1365-2672

pii

JAM3018

journal_volume

101

pub_type

杂志文章
  • Characterization of filamentous bacteriophage PE226 infecting Ralstonia solanacearum strains.

    abstract:AIMS:The aim of this study was to isolate and characterize new bacteriophages that infect a wide range of plant pathogenic Ralstonia solanacearum strains. METHODS AND RESULTS:Fifteen bacteriophages were isolated from pepper, tomato and tobacco plant rhizospheres infected with R. solanacearum. A host specificity analys...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04882.x

    authors: Murugaiyan S,Bae JY,Wu J,Lee SD,Um HY,Choi HK,Chung E,Lee JH,Lee SW

    更新日期:2011-01-01 00:00:00

  • The intrinsic cephalosporin resistome of Listeria monocytogenes in the context of stress response, gene regulation, pathogenesis and therapeutics.

    abstract::Intrinsic resistance to antibiotics is a serious therapeutic problem in the case of many bacterial species. The Gram-positive human pathogen Listeria monocytogenes is intrinsically resistant to broad spectrum cephalosporin antibiotics, which are commonly used in therapy of bacterial infections. Besides three penicilli...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.12989

    authors: Krawczyk-Balska A,Markiewicz Z

    更新日期:2016-02-01 00:00:00

  • Genetically modified bacteriophages in applied microbiology.

    abstract::Bacteriophages represent a simple viral model of basic research with many possibilities for practical application. Due to their ability to infect and kill bacteria, their potential in the treatment of bacterial infection has been examined since their discovery. With advances in molecular biology and gene engineering, ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13207

    authors: Bárdy P,Pantůček R,Benešík M,Doškař J

    更新日期:2016-09-01 00:00:00

  • Effect of crop management and sample year on abundance of soil bacterial communities in organic and conventional cropping systems.

    abstract:AIMS:To identify changes in the bacterial community, at the phylum level brought about by varied crop management. METHODS AND RESULTS:Next-generation sequencing methods were used to compare the taxonomic structure of the bacterial community within 24 agricultural soils managed with either organic or conventional metho...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12822

    authors: Orr CH,Stewart CJ,Leifert C,Cooper JM,Cummings SP

    更新日期:2015-07-01 00:00:00

  • Strain- and matrix-dependent adhesion of Lactobacillus plantarum is mediated by proteinaceous bacterial compounds.

    abstract:AIMS:The ability of 31 Lactobacillus plantarum strains to adhere to biological matrixes was evaluated, and the molecules involved in adherence were studied. METHODS AND RESULTS:Mucin, basement membrane proteins and Caco-2 cells were used in adhesion tests. These in vitro assays, together with a yeast agglutination tes...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.03086.x

    authors: Tallon R,Arias S,Bressollier P,Urdaci MC

    更新日期:2007-02-01 00:00:00

  • Rotavirus vaccine stability in the aquatic environment.

    abstract:AIMS:To evaluate the thermal and length of stability of the Rotaviruses (RV) vaccine (RotaTeq) in the aquatic environment. METHODS AND RESULTS:Surface freshwater, brackish and drinking water were spiked with RV vaccine strain and stored at 22 and 4°C. The virus infectivity and genome persistence were evaluated by plaq...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13021

    authors: Moresco V,Damazo NA,Barardi CR

    更新日期:2016-02-01 00:00:00

  • Lactic acid bacteria associated with a heat-processed pork product and sources of variation affecting chemical indices of spoilage and sensory characteristics.

    abstract:AIMS:To evaluate the potential for developing a quality index for a Danish modified atmosphere packaged (MAP) heat-processed and naturally contaminated pork meat product stored at 5 degrees C. METHODS AND RESULTS:The composition of the predominating microflora and changes in contents of tyramine, arginine, organic aci...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.04045.x

    authors: Laursen BG,Byrne DV,Kirkegaard JB,Leisner JJ

    更新日期:2009-02-01 00:00:00

  • Biofilm problems in dental unit water systems and its practical control.

    abstract::Dental chair units (DCUs) contain integrated systems that provide the instruments and services for a wide range of dental procedures. DCUs use water to cool and irrigate DCU-supplied instruments and tooth surfaces during dental treatment. Water is supplied to these instruments by a network of interconnected narrow-bor...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2008.04100.x

    authors: Coleman DC,O'Donnell MJ,Shore AC,Russell RJ

    更新日期:2009-05-01 00:00:00

  • Upgrading the phenolic content, antioxidant and antimicrobial activities of garden cress seeds using solid-state fermentation by Trichoderma reesei.

    abstract:AIMS:This study aims to evaluate the impact of solid-state fermentation (SSF) by Trichoderma reesei on the phenolic content, antioxidant and antimicrobial activities of garden cress seeds (GCS). METHODS AND RESULTS:The factorial statistical design was employed to optimize the SSF conditions, incubation time, pH, tempe...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14394

    authors: Abdel-Aty AM,Bassuiny RI,Barakat AZ,Mohamed SA

    更新日期:2019-11-01 00:00:00

  • FT-IR microspectroscopy for early identification of some clinically relevant pathogens.

    abstract:AIMS:To investigate the potentials and limitations of Fourier transform-infrared (FT-IR) microspectroscopy as a tool to identify, at the level of microcolonies, pathogenic bacteria frequently isolated in the clinical environment. METHODS AND RESULTS:A total of 1570 FT-IR spectra from 164 gram-positive and gram-negativ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02969.x

    authors: Sandt C,Madoulet C,Kohler A,Allouch P,De Champs C,Manfait M,Sockalingum GD

    更新日期:2006-10-01 00:00:00

  • A set of PCRs for rapid identification and characterization of Pseudomonas syringae phylogroups.

    abstract:AIMS:The aim of this study was to develop a rapid PCR-based method for the specific detection of individual phylogroups of the Pseudomonas syringae complex. METHODS AND RESULTS:Seven primer pairs were developed by analysing whole genomes of 54 Ps. syringae strains. The specificity and sensitivity of these primer pairs...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13017

    authors: Borschinger B,Bartoli C,Chandeysson C,Guilbaud C,Parisi L,Bourgeay JF,Buisson E,Morris CE

    更新日期:2016-03-01 00:00:00

  • Discovery of anti-microbial and anti-tubercular molecules from Fusarium solani: an endophyte of Glycyrrhiza glabra.

    abstract:AIMS:Glycyrrhiza glabra is a high-value medicinal plant thriving in biodiversity rich Kashmir Himalaya. The present study was designed to explore the fungal endophytes from G. glabra as a source of bioactive molecules. METHODS AND RESULTS:The extracts prepared from the isolated endophytes were evaluated for anti-micro...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13410

    authors: Shah A,Rather MA,Hassan QP,Aga MA,Mushtaq S,Shah AM,Hussain A,Baba SA,Ahmad Z

    更新日期:2017-05-01 00:00:00

  • Development of a silver-based dual-function antimicrobial laundry additive and textile coating for the decontamination of healthcare laundry.

    abstract:AIMS:To repurpose a silver-based antimicrobial textile coating product (Micro-Fresh 1911) as a dual-function antimicrobial laundry additive and textile coating. METHODS AND RESULTS:Survival of Escherichia coli or Staphylococcus aureus type and clinical isolates in a domestic 40°C wash was assessed with and without soi...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14850

    authors: Owen L,Laird K

    更新日期:2020-09-11 00:00:00

  • World Health Organization activities on anthrax surveillance and control.

    abstract::The achievements of a World Health Organization Anthrax Working Group, established in 1990, have been the production of two editions of guidelines on anthrax surveillance and control and the formulation of templates to assist countries in the construction of their surveillance and control programmes. The latter was ma...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00903.x

    authors: Turnbull PC,Hugh-Jones ME,Cosivi O

    更新日期:1999-08-01 00:00:00

  • Aerobic Cr(VI) reduction by Thermus scotoductus strain SA-01.

    abstract:AIM:To evaluate Thermus scotoductus SA-01's ability to reduce Cr(VI) aerobically. METHODS AND RESULTS:T. scotoductus SA-01 is able to reduce Cr(VI) aerobically when grown in a complex organic medium containing Cr(VI) concentrations up to 0.5 mmol l(-1). Suspension of T. scotoductus SA-01 cells also reduced Cr(VI) aero...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03429.x

    authors: Opperman DJ,van Heerden E

    更新日期:2007-11-01 00:00:00

  • Metalworking fluids biodiversity characterization.

    abstract:AIMS:Hypersensitivity pneumonitis of machinists associated with metalworking fluids (MWF) was recently linked to Mycobacterium immunogenum. In addition to Mycobacterium, impacts of continuous and massive contact to other micro-organisms, such as Pseudomonas, were little studied. This report intended to quantify and cha...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04433.x

    authors: Gilbert Y,Veillette M,Duchaine C

    更新日期:2010-02-01 00:00:00

  • Effects of sodium bisulfate on the bacterial population structure of dairy cow waste.

    abstract:AIMS:To determine the effects of sodium bisulfate (SBS) on the bacterial populations in cattle waste. METHODS AND RESULTS:We applied SBS at 0, 60, 70 or 100 kg week(-1) to cattle waste as it accumulated on the floors of four cattle pens, housing eight cattle each. We observed significant pH decreases in all of the tre...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05047.x

    authors: McGarvey JA,Stackhouse KR,Miller WG,Stanker LH,Hnasko R,Mitloehner F

    更新日期:2011-08-01 00:00:00

  • The antimicrobial effects of glucosinolates and their respective enzymatic hydrolysis products on bacteria isolated from the human intestinal tract.

    abstract:AIMS:The aim of the study was to evaluate the in vitro antibacterial activity of glucosinolates and their enzymatic hydrolysis product against bacteria isolated from the human intestinal tract. METHODS AND RESULTS:Using a disc diffusion bioassay, different doses of intact glucosinolates and their corresponding hydroly...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04180.x

    authors: Aires A,Mota VR,Saavedra MJ,Rosa EA,Bennett RN

    更新日期:2009-06-01 00:00:00

  • Killing of spores of Bacillus species by cetyltrimethylammonium bromide.

    abstract:AIMS:To investigate effects of the cationic surfactant cetyltrimethylammonium bromide (CTAB), a disinfectant, on spores of Bacillus species. METHODS AND RESULTS:The ability of CTAB to trigger release of Bacillus spores' large depot of dipicolinic acid (DPA) in a 1 : 1 chelate with Ca2+ (CaDPA), and to kill spores was ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14242

    authors: Dong W,Green J,Korza G,Setlow P

    更新日期:2019-05-01 00:00:00

  • In vitro effect of phenolic antioxidants on germination, growth and aflatoxin B accumulation by peanut Aspergillus section Flavi.

    abstract:AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was de...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02661.x

    authors: Passone MA,Resnik SL,Etcheverry MG

    更新日期:2005-01-01 00:00:00

  • Activities of muscadine grape skin and quercetin against Helicobacter pylori infection in mice.

    abstract:AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04870.x

    authors: Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

    更新日期:2011-01-01 00:00:00

  • Analysis of gyrA mutations in quinolone-resistant and -susceptible Campylobacter jejuni isolates from retail poultry and human clinical isolates by non-radioactive single-strand conformation polymorphism analysis and DNA sequencing.

    abstract:AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02242.x

    authors: Beckmann L,Müller M,Luber P,Schrader C,Bartelt E,Klein G

    更新日期:2004-01-01 00:00:00

  • Influence of isomalto-oligosaccharides on intestinal microbiota in rats.

    abstract:AIMS:Isomalto-oligosaccharides (IMO) with α(1 --> 6) and α(1 --> 4) glucosidic linkages are produced by enzymatic conversion of starch. IMO are only partially digestible but data on their influence on intestinal microbiota are limited. It was the aim of this study to investigate the effect of IMO diet on intestinal mic...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04984.x

    authors: Ketabi A,Dieleman LA,Gänzle MG

    更新日期:2011-05-01 00:00:00

  • Automated dead-end ultrafiltration of large volume water samples to enable detection of low-level targets and reduce sample variability.

    abstract:AIMS:A Portable Multi-use Automated Concentration System (PMACS) concentrates micro-organisms from large volumes of water through automated dead-end ultrafiltration and backflushing. The ability to detect microbial targets from ground, surface and cooling tower waters collected using standard methods was compared with ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05345.x

    authors: Leskinen SD,Kearns EA,Jones WL,Miller RS,Bevitas CR,Kingsley MT,Brigmon RL,Lim DV

    更新日期:2012-08-01 00:00:00

  • Solar UV reduces Cryptosporidium parvum oocyst infectivity in environmental waters.

    abstract:AIMS:To determine the effect of solar radiation on Cryptosporidium parvum in tap and environmental waters. METHODS AND RESULTS:Outdoor tank experiments and a cell culture infectivity assay were used to measure solar inactivation of C. parvum oocysts in different waters. Experiments conducted on days with different lev...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03658.x

    authors: King BJ,Hoefel D,Daminato DP,Fanok S,Monis PT

    更新日期:2008-05-01 00:00:00

  • Modelling of factors influencing the effect of osmotic solution on reduction of selected microorganisms.

    abstract:AIMS:The goal of this research is to model the effects of two osmotic solutions factors on the reduction of selected microorganisms, and to assess the application in osmotic dehydration process of animal raw materials from the aspect of microbiological safety. METHODS AND RESULTS:Sugar beet molasses and aquatic osmoti...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13927

    authors: Filipović I,Markov S,Filipović V,Filipović J,Vidaković A,Novković N,Rafajlovska V

    更新日期:2018-09-01 00:00:00

  • Molecular characterization of Oenococcus oeni genes encoding proteins involved in arginine transport.

    abstract:AIMS:This work was carried out to complete the sequence of the arc cluster involved in arginine catabolism in Oenococcus oeni, and particularly to characterize the genes encoding proteins involved in arginine transport. METHODS AND RESULTS:Using molecular cloning, two loci encoding proteins involved in the arginine-or...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.01907.x

    authors: Divol B,Tonon T,Morichon S,Gindreau E,Lonvaud-Funel A

    更新日期:2003-01-01 00:00:00

  • Purification and characterization of a bacteriocin-like compound (Lichenin) produced anaerobically by Bacillus licheniformis isolated from water buffalo.

    abstract:AIMS:To characterize a bacteriocin-like factor from Bacillus licheniformis 26 L-10/3RA isolated from buffalo rumen. METHODS AND RESULTS:The culture supernatant exhibited the antibacterial activity against a number of indicator organisms in a cut-well agar assay under anaerobic conditions. The inhibitory component was ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01429.x

    authors: Pattnaik P,Kaushik JK,Grover S,Batish VK

    更新日期:2001-10-01 00:00:00

  • Kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting.

    abstract:AIMS:  To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. METHODS AND RESULTS:  The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluor...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04964.x

    authors: Pawelczyk S,Bumann D,Abraham WR

    更新日期:2011-04-01 00:00:00

  • In vitro antibacterial activity and antifungal mode of action of flocculosin, a membrane-active cellobiose lipid.

    abstract:AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04280.x

    authors: Mimee B,Pelletier R,Bélanger RR

    更新日期:2009-09-01 00:00:00