Abstract:
AIMS:To investigate effects of the cationic surfactant cetyltrimethylammonium bromide (CTAB), a disinfectant, on spores of Bacillus species. METHODS AND RESULTS:The ability of CTAB to trigger release of Bacillus spores' large depot of dipicolinic acid (DPA) in a 1 : 1 chelate with Ca2+ (CaDPA), and to kill spores was investigated. CTAB-triggered CaDPA release from spores of Bacillus subtilis, Bacillus cereus and Bacillus megaterium, but was not followed by completion of germination. CaDPA release triggered by CTAB increased at higher temperatures, and was optimal for B. subtilis spores at pH 9·4 and 30 μg ml-1 CTAB. CTAB also killed Bacillus spores as shown by plate counts and vital staining of treated dormant spores, and after their germination. However, B. cereus and B. megaterium spores were more CTAB-sensitive than were B. subtilis spores. CaDPA release from and killing of CTAB-treated spores of isogenic B. subtilis mutants lacking germination proteins was also examined, and compared with effects of the well-known germinant dodecylamine on spores, to determine how CTAB exerts its effects on spores. CONCLUSIONS:The results of this investigation showed that CTAB kills spores of three Bacillus species, perhaps by damaging the spore inner membrane, although it is also possible that some killing by this agent follows its triggering of spore germination. SIGNIFICANCE AND IMPACT OF THE STUDY:The results of this work indicate that CTAB is also a disinfectant, but also a sporicide, and may be a useful adjunct in spore decontamination, especially at higher temperatures.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Dong W,Green J,Korza G,Setlow Pdoi
10.1111/jam.14242subject
Has Abstractpub_date
2019-05-01 00:00:00pages
1391-1401issue
5eissn
1364-5072issn
1365-2672journal_volume
126pub_type
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00261.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2006.03017.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01054.x
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2002.01771.x
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更新日期:2002-01-01 00:00:00
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2002-01-01 00:00:00
abstract::An extracellular alpha-amylase gene from the hyperthermophilic archaeon Pyrococcus woesei has been cloned and sequenced. The 1.4-kb protein-coding sequence is identical to that of the corresponding alpha-amylase gene of the closely related species P. furiosus. By using a shuttle cloning vector for halophilic bacteria,...
journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.00988.x
更新日期:2000-03-01 00:00:00