Abstract:
AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was determined. METHODS AND RESULTS:Assays on the lag phase of germination, germination percentage, germ tube elongation rate, lag phase, growth rate and AFB(1) production by three strains of Aspergillus flavus and three of Aspergillus parasiticus were carried out in vitro on peanut extract meal agar conditioned at different water activities (a(w): 0.982, 0.971, 0.955, 0.937). The antioxidants PP and BHA efficiently inhibited the germination of the two species tested at the doses 10 and 20 mmol(-1). The antioxidants PP and BHA at 1 mmol l(-1) and THB at 20 mmol l(-1) reduced the germ tube elongation rate most effectively, regardless of a(w) levels. An increase in the lag time and a reduction in the growth rate of 100% of the strains was observed, this was due to the action of BHT at the doses 10 and 20 mmol(-1) at 0.982, 0.971 and 0.955 a(w), although these treatments stimulated the AFB(1) accumulation in most of the fungi tested. The more effective antioxidants were PP and BHA, which increased the lag phase, reduced the growth rate and AFB(1) production in all of the strains at the four a(w) assayed. At concentrations 10 and 20 mmol l(-1), these antioxidants totally inhibited fungal development. CONCLUSIONS:The study shows that the antioxidants BHA and PP are effective fungal inhibitors to peanut Aspergillus section Flavi in wide range of water activity. SIGNIFICANCE AND IMPACT OF THE STUDY:The results suggest that phenolic antioxidants, BHA and PP, can be effective fungitoxicants on aflatoxigenic strains in peanut at industrial level.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Passone MA,Resnik SL,Etcheverry MGdoi
10.1111/j.1365-2672.2005.02661.xsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
682-91issue
3eissn
1364-5072issn
1365-2672pii
JAM2661journal_volume
99pub_type
杂志文章abstract:AIMS:To determine the effects of sodium bisulfate (SBS) on the bacterial populations in cattle waste. METHODS AND RESULTS:We applied SBS at 0, 60, 70 or 100 kg week(-1) to cattle waste as it accumulated on the floors of four cattle pens, housing eight cattle each. We observed significant pH decreases in all of the tre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05047.x
更新日期:2011-08-01 00:00:00
abstract:AIMS:Lactic acid bacteria (LAB) were isolated and sequenced from the faeces of healthy dogs. Five of these strains were selected and further characterized to clarify the potential of these strains as probiotics for canine. METHODS AND RESULTS:LAB were found in 67% (14/21) of the canine faeces samples when plated on La...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02884.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:The purpose of this study was to evaluate the ISO 16266 membrane filtration culture-based method for the detection of Pseudomonas aeruginosa from cooling tower and related water samples and to compare the performance of the Pseudalert MPN method (Pseudalert™/Quanti-Tray® ) for the enumeration of P. aeruginosa with...
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更新日期:2020-06-01 00:00:00
abstract:AIMS:Our aim was to assess the diversity of the nutrient germination response of Bacillus cereus spores. METHODS AND RESULTS:B. cereus spore germination was monitored by decrease in optical density using a Bioscreen C analyser in response to the major germinant substances inosine and l-alanine. Spores of a set of 12 s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03847.x
更新日期:2008-10-01 00:00:00
abstract:AIMS:To investigate the genetic basis of Cr(VI) resistance and its reduction to Cr(III) in indigenous bacteria isolated from tannery effluent. METHODS AND RESULTS:Four bacteria resistant to high Cr(VI) levels were isolated and identified as Bacillus spp. Their Cr(VI) reduction ability was tested. To assess the genetic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04326.x
更新日期:2009-11-01 00:00:00
abstract:AIMS: In this study, we compare seven different methods which have been designed or modified to extract total DNA from raw milk and raw milk cheese with a view to its subsequent use for the PCR of bacterial DNA. MATERIALS AND RESULTS: Seven extraction methods were employed to extract total DNA from these foods, and ...
journal_title:Journal of applied microbiology
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doi:10.1111/j.1365-2672.2012.05294.x
更新日期:2012-07-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the effect of ethanol exposure on the expression level of geranylgeranyl pyrophosphate synthase gene involved in the metabolism of Oenococcus oeni to probe the mechanisms of ethanol tolerance correlated with adaptive changes. METHODS AND RESULTS:The evaluation of ten poten...
journal_title:Journal of applied microbiology
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更新日期:2014-01-01 00:00:00
abstract:AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2009-07-01 00:00:00
abstract:AIM:Physiological race determination of 143 Fusarium oxysporum f. sp. lycopersici (FOL) isolates collected along 30 years in major tomato-producing regions of Brazil. MATERIALS AND RESULTS:Physiological races were determined via root-dipping inoculation of differential tomato accessions and by the PCR-based marker sys...
journal_title:Journal of applied microbiology
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doi:10.1111/jam.14966
更新日期:2020-12-11 00:00:00
abstract::Traditional microbiological methods are still used extensively for analysis of micro-organisms in water. However, they are inefficient due to a high labour input requirement, a low sample capacity, and often a long time lag before results are available. Analytical stages involving incubation and growth (enrichments an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2672.1998.tb05300.x
更新日期:1998-12-01 00:00:00
abstract:AIMS:To develop a PCR-based method for reliable detection of Escherichia coli that enables its differentiation from biochemically and phylogenetically related bacteria. METHODS AND RESULTS:Using multiplex PCR targeting four genes (cytochrome bd complex, lactose permease, beta-d-glucuronidase, and beta-d-galactosidase)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03838.x
更新日期:2008-10-01 00:00:00
abstract:AIM:In this study, we investigated the effect of plagiochin E (PLE), a botanic-derived phenolic natural product, on reversal of fungal resistance to fluconazole (FLC) in vitro and the related mechanism. METHODS AND RESULTS:A synergistic action of PLE and FLC was observed in the FLC-resistant Candida albicans strains a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03617.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:The aims of this study were to characterize the molecular variations in the quinolone resistance-determining region (QRDR) of gyrA among quinolone-resistant and -susceptible Campylobacter jejuni isolates originating from foods of animal origin and human infections and to evaluate the suitability of the single-stra...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02242.x
更新日期:2004-01-01 00:00:00
abstract::The influence of growth phase on the evolution of cellular fatty acids (CFA) and survival of Sinorhizobium and Bradyrhizobium during desiccation and storage at different levels of relative humidity (R. H.) was studied. Lactobacillic, cis vaccenic and palmitic acids were the major fatty acids of S. meliloti RCR 2011, B...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00860.x
更新日期:1999-10-01 00:00:00
abstract::Nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (CLA) from free linoleic acid in vitro. Two strains of Propionibacterium freudenreichii ssp. freudenreichii and one strain ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00481.x
更新日期:1998-07-01 00:00:00
abstract:AIMS:In this study, pulsed-electric fields (PEF) and high-voltage electrical discharges (HVED) are proposed as new techniques for the microbial stabilization of red wines before bottling. The efficiency of the treatment was then evaluated. METHODS AND RESULTS:PEF and HVED-treatments have been applied to wine for the i...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12981
更新日期:2016-01-01 00:00:00
abstract:AIMS:The objective of this study was the fundamental investigation of the antimicrobial efficiency of various hop extracts against selected foodborne pathogens in vitro, as well as their activity against Listeria monocytogenes in a model meat marinade and on marinated pork tenderloins. METHODS AND RESULTS:In a first s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12717
更新日期:2015-03-01 00:00:00
abstract:AIMS:To characterize the two-component cell lysis cassette comprised of holin (Hyb5) and endolysin (Lyb5) encoded by Lactobacillus fermentum temperate bacteriophage phiPYB5, and illustrate the potential application of Lyb5 as therapeutic agents. METHODS AND RESULTS:The hyb5-lyb5 cassette was cloned from the genome lib...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03953.x
更新日期:2008-12-01 00:00:00
abstract:AIM:To develop co-aggregated bacterial inoculant comprising of Methylobacterium oryzae CBMB20/Methylobacterium suomiense CBMB120 strains with Azospirillum brasilense (CW903) strain and testing their efficiency as inoculants for plant growth promotion (PGP). METHODS AND RESULTS:Biofilm formation and co-aggregation effi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12384
更新日期:2014-02-01 00:00:00
abstract::We report a quantitative PCR which utilizes primers from a conserved 23S rDNA sequence identified in nine different spoilage bacteria commonly present in meat. The PCR detected the spoilage bacteria by amplifying a specific 207 bp sequence from their chromosomal DNA. Quantification of PCR product by electrochemilumine...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1997.00368.x
更新日期:1997-03-01 00:00:00
abstract:AIMS:To analyse genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an 8-year period and to assess the relative contribution of mutation and recombination in shaping their diversity. METHODS AND RESULTS:By using random amplified polymorphic DNA (RAPD) P...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12858
更新日期:2015-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2019-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01294.x
更新日期:2001-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04700.x
更新日期:2010-07-01 00:00:00
abstract:AIMS:To assess the inhibitory activity and the influence of culture condition on the growth and bacteriocin, Thermophilin 1277, production by Streptococcus thermophilus SBT1277. METHODS AND RESULTS:Thermophilin 1277, which was produced by S. thermophilus SBT1277, showed an antimicrobial activity against several lactic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03159.x
更新日期:2007-04-01 00:00:00
abstract:AIMS:To investigate the microbiota in marinated, vacuum-packed pork and to characterize isolated bacteria with regard to their spoilage potential. METHODS AND RESULTS:Laboratory marinated pork meat and commercial products from three Norwegian producers were examined. Lactic acid bacteria dominated in all products at t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04183.x
更新日期:2009-06-01 00:00:00
abstract::The volatile oils of black pepper [Piper nigrum L. (Piperaceae)], clove [Syzygium aromaticum (L.) Merr. & Perry (Myrtaceae)], geranium [Pelargonium graveolens L'Herit (Geraniaceae)], nutmeg [Myristica fragrans Houtt. (Myristicaceae), oregano [Origanum vulgare ssp. hirtum (Link) Letsw. (Lamiaceae)] and thyme [Thymus vu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00969.x
更新日期:2000-02-01 00:00:00
abstract:AIMS:To add a spore germination step in order to reduce decontamination temperature and time requirements compared to the current hot, humid air decontamination parameters, which are 75-80°C, ≥72 h, 70-90% RH, down to ≤60°C and ≤24 h total decontamination time. METHODS AND RESULTS:Bacillus anthracis spore germination ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14474
更新日期:2020-01-01 00:00:00
abstract::The goals of this review are to summarize the current knowledge on the application of Lactobacillus salivarius as a probiotic in animals and humans, and to address safety concerns with its use on live hosts. Overall, several strains of L. salivarius are well established probiotics with multiple applications in animal ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.13438
更新日期:2017-07-01 00:00:00
abstract:AIMS:This study focuses on the bactericidal mechanism of the new combination of disinfectant glutaraldehyde-didecyldimethylammonium bromide (GA-DDAB) against Escherichia coli. METHODS AND RESULTS:Escherichia coli were exposed to GA-DDAB, and assays for cell morphology, K+ and Ca2+ leakage, H+ -ATPase activity and DNA ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13384
更新日期:2017-03-01 00:00:00