Abstract:
AIMS:The present study focuses on the impact of two different drying technologies and the influence of protectants on process survival and storage stability of the two lactic acid bacterial strains Enterococcus faecium and Lactobacillus plantarum. METHODS AND RESULTS:After incubation with the protectants glucose, sucrose, trehalose, and maltodextrin the concentrated bacterial suspensions were subjected to fluidized bed drying and lyophilization and subsequently stored at 4, 22, and 35 degrees C for half a year. Lactobacillus plantarum turned out to be more sensitive to both drying methods than Ent. faecium. Without the addition of a protectant cells of both strains suffered higher losses during fluidized bed drying. Elevated storage temperatures correlate with a higher decline of viable bacterial cells. CONCLUSIONS:Although survival rates varied between the strains, the nonreducing disaccharides revealed overall best protection for both investigated lactic acid bacteria during processing and storage. The addition of protective carbohydrates can prevent the decline in viability during fluidized bed drying. SIGNIFICANCE AND IMPACT OF THE STUDY:The influence of protectants proved to be species specific and therefore needs to be determined on a case-to-case basis. Survival rates, duration, and energy consumption appear to be the crucial parameters to evaluate the economy of production processes for industrial starter cultures.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Strasser S,Neureiter M,Geppl M,Braun R,Danner Hdoi
10.1111/j.1365-2672.2009.04192.xsubject
Has Abstractpub_date
2009-07-01 00:00:00pages
167-77issue
1eissn
1364-5072issn
1365-2672pii
JAM4192journal_volume
107pub_type
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