Abstract:
AIM:Assess performance of broth microdilution (BMD) as well as agar dilution methods for antimicrobial susceptibility testing of Staphylococci using tetrazolium salt. METHODS AND RESULTS:Minimum inhibitory concentration (MIC) of eight antimicrobials; vancomycin (VA), linezolid, oxacillin, gentamicin (CN), tetracycline, ciprofloxacin, erythromycin and clindamycin was investigated for 80 isolates of Staphylococci by BMD with the addition of dimethyl thiazole diphenyl tetrazolium bromide (MTT), agar dilution with the addition of MTT and triphenyl tetrazolium chloride at the standard bacterial concentration together with addition of MTT at an experimental bacterial concentration. BMD (MTT) showed the highest agreement in comparison with the standard BMD. CONCLUSIONS:Colorimetric BMD was rapid and easy to interpret. Colorimetric agar dilution (MTT) was less tedious than BMD. SIGNIFICANCE AND IMPACT OF THE STUDY:Colorimetric antibiotic susceptibility is a good option to provide rapid reliable results for critically ill patients. In addition, agar dilution (MTT) helps to investigate outbreaks of methicillin-resistant Staphylococcus aureus (MRSA), VISA or VRSA. BMD (MTT) can be performed routinely to detect VA MIC in MRSA blood stream infections and hospital acquired pneumonia, where, high VA MIC is associated with a higher mortality rate.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Mahmoud BS,ElMasry SA,Fahim NAEMM,Abd ElSattar MA,Shaker OAdoi
10.1111/jam.14347subject
Has Abstractpub_date
2019-09-01 00:00:00pages
693-700issue
3eissn
1364-5072issn
1365-2672journal_volume
127pub_type
杂志文章abstract:AIMS:The aims of this study were to identify the Fusarium isolates based on translation elongation factor (tef) 1α sequence, to determine the genetic diversity among isolates and species using selected microsatellite markers and to examine the pathogenicity of Fusarium isolates causing fruit rot disease of banana. MET...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13582
更新日期:2017-12-01 00:00:00
abstract:AIMS:To establish a laboratory model to compare the effectiveness of detergent-based disinfection procedures for reducing cross-contamination risks during handling of contaminated chicken. METHODS AND RESULTS:During handling of chickens, artificially contaminated with Salmonella enteritidis PT4, the organism was widel...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02127.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To identify dominant bacteria in grain (barley)-fed cattle for isolation and future use to increase the efficiency of starch utilization in these cattle. METHODS AND RESULTS:Total DNA was extracted from samples of the rumen contents from eight steers fed a barley diet for 9 and 14 days. Bacterial profiles were ob...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03492.x
更新日期:2007-12-01 00:00:00
abstract::Thirty-six strains of Escherichia coli isolated from animals in Bario, a remote area in Sarawak, Malaysia, were examined for presence of plasmid DNA and their susceptibility to nine antimicrobial agents. Of the total 36 isolates, five bovine and six canine isolates were found to contain plasmid DNA ranging in sizes fr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract:AIMS:To evaluate ATP bioluminescence-based hygiene monitoring systems under conditions relevant to fish processing environments. METHODS AND RESULTS:The ATP bioluminescence of fish fractions that are potentially present after insufficient cleaning of fish processing environments was determined. Different fractions and...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14292
更新日期:2019-07-01 00:00:00
abstract:AIMS:Chlamydia trachomatis and herpes simplex virus (HSV) are the most prevalent bacterial and viral sexually transmitted infections. Due to the chronic nature of their infections, they are able to interact with titanium-dioxide (TiO2 ) nanoparticles (NPs) applied as food additives or drug delivery vehicles. The aim of...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13560
更新日期:2017-11-01 00:00:00
abstract:AIMS:To evaluate archaeal diversity in natural and impacted habitats from Rio de Janeiro state, Brazil, a tropical region of South America. METHODS AND RESULTS:16S rRNA gene was amplified directly by polymerase chain reaction (PCR) from genomic DNA, extracted from Guanabara Bay (GB) water, halomarine sediment (HS), mu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03230.x
更新日期:2007-07-01 00:00:00
abstract:AIMS:To develop an effective multiplex polymerase chain reaction (PCR) for the simultaneous detection of three important Vibrio species, Vibrio cholerae (Vc), V. parahaemolyticus (Vp) and V. vulnificus (Vv) using the groEL gene, a potential phylogenetic marker. METHODS AND RESULTS:Three species-specific primer sets we...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12056
更新日期:2013-02-01 00:00:00
abstract::The changes in bacterial counts during the storage of a natural mineral water from a French spring were studied. Samples were taken from the spring and the bottling line. Viable cultivable (VC) bacteria were counted on R2A medium. Total counts, viable and dead bacteria were counted using the LIVE/DEAD Bac Light VIABIL...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12675
更新日期:2015-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01323.x
更新日期:2001-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13448
更新日期:2017-06-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04059.x
更新日期:2009-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13382
更新日期:2017-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00412.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) ,...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04820.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:To examine associations between growth rate within bacterial populations and survival patterns following treatment with antimicrobial agents. METHODS AND RESULTS:Time survival data were generated for the inactivation of Escherichia coli populations, grown as batch and continuous cultures, exposed to ciprofloxacin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02079.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:The aim was to isolate, identify and characterize yeasts present in rumen fluid and to select strains showing potential as probiotics. METHODS AND RESULTS:Rumen fluid was sampled from 4 herds of dairy and beef cattle and 77 yeast isolates were identified. Initial screening was based on the capacity to maintain vi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14350
更新日期:2019-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.853464.x
更新日期:1998-09-01 00:00:00
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journal_title:Journal of applied microbiology
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abstract:AIMS:Vibrio vulnificus is a common bacterial pathogen causing haemorrhagic septicaemia in eel farming. This study investigates the immunogenicity of an outer membrane protein U (OmpU) of V. vulnificus and the feasibility of the protein as a new subunit vaccine against V. vulnificus. METHODS AND RESULTS:Partial gene se...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-08-14 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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更新日期:2010-03-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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更新日期:2016-02-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01468.x
更新日期:2001-12-01 00:00:00
abstract:AIM:The aim of this study was to characterize the microbial community involved in anaerobic degradation of petroleum hydrocarbon under low- and moderate-temperature conditions. METHODS AND RESULTS:Sulfate-reducing enrichment cultures growing on crude oil and p-xylene were established at low and moderate temperatures. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04886.x
更新日期:2011-01-01 00:00:00
abstract:AIM:To evaluate the bacterial composition of collared peccary semen and foreskin mucosa, and to verify the sensitivity of isolates to antimicrobials used in semen conservation and to Aloe vera gel, which is an alternative external cryoprotectant. METHODS AND RESULTS:Nine foreskin mucosa and ejaculate samples from adul...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14663
更新日期:2020-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01021.x
更新日期:2000-04-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14394
更新日期:2019-11-01 00:00:00
abstract::A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00455.x
更新日期:1998-06-01 00:00:00