Abstract:
AIMS:This project aimed to investigate the mechanism of attachment of the vegetative cells and spores of thermophilic bacilli to stainless steel with a view to devising strategies to limit biofilm development and survival. METHODS AND RESULTS:Spores and vegetative cells of bacterial isolates were exposed to protein denaturing agents (sodium dodecyl sulphate (SDS) and trypsin) and polysaccharide removing agents (sodium metaperiodate, trichloroacetic acid (TCA) and lysozyme). Treatment with sodium metaperiodate, TCA and lysozyme increased the number of vegetative cells attaching in many of the strains studied, while SDS and trypsin decreased attachment. Spores attached to stainless steel in greater numbers than vegetative cells, and the various treatments had less effect on this attachment than for vegetative cells. Viability of the cells or spores was not an important factor in attachment, as cells and spores rendered non-viable also attached to stainless steel in similar numbers. Coating the stainless steel with skim milk proteins decreased the attachment of both vegetative cells and spores. There was no correlation between the degree of attachment and the amount of extracellular polysaccharide (EPS) produced by each strain, surface hydrophobicity or zeta potential of vegetative cells or spores, though spores were found to be more hydrophobic than vegetative cells. CONCLUSIONS:The results suggest that biofilm formation by these thermophilic bacilli is probably a multifactorial process, and that cell-surface proteins play a very important role in the initial process of attachment during the formation of biofilms by these bacteria. SIGNIFICANCE AND IMPACT OF THE STUDY:This information will provide direction for developing improved cleaning systems to control biofilms of thermophilic bacilli in dairy manufacturing plants.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Parkar SG,Flint SH,Palmer JS,Brooks JDdoi
10.1046/j.1365-2672.2001.01323.xsubject
Has Abstractpub_date
2001-06-01 00:00:00pages
901-8issue
6eissn
1364-5072issn
1365-2672pii
jam1323journal_volume
90pub_type
杂志文章abstract:AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04440.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:The objective of this study was to compare qualitatively and quantitatively the results of identification of the bacteria present in milk samples from cows with subclinical mastitis using multiplex qPCR assay and matrix-assisted laser desorption-ionization time of flight mass spectrometry (MALDI-TOF MS® ) after ba...
journal_title:Journal of applied microbiology
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更新日期:2019-09-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2010-03-01 00:00:00
abstract:AIMS:We evaluated the effects of Bacillus anthracis surrogates, low surface concentrations, surface materials and assay methods on false-negative rate (FNR) and limit of detection (LOD95 ) for recovering Bacillus spores using a macrofoam-swab sampling procedure. METHODS AND RESULTS:Bacillus anthracis Sterne or Bacillu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2018-05-01 00:00:00
abstract:AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...
journal_title:Journal of applied microbiology
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更新日期:2017-01-01 00:00:00
abstract:AIMS:To monitor emissions of NH(3) and N(2)O during composting and link these to ammonia oxidation rates and the community structure of ammonia oxidizing bacteria (AOB). METHODS AND RESULTS:A laboratory-scale compost reactor treating organic household waste was run for 2 months. NH(3) emissions peaked when pH started ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04111.x
更新日期:2009-05-01 00:00:00
abstract:AIMS:Application of molecular techniques to ecological studies has unveiled a wide diversity of micro-organisms in natural communities, previously unknown to microbial ecologists. New lineages of Archaea were retrieved from several non-extreme environments, showing that these micro-organisms are present in a large vari...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01297.x
更新日期:2001-05-01 00:00:00
abstract:AIMS:Changes in fermentation pattern during the treatment of organic wastes containing solid materials by thermophilic anaerobic microflora were investigated with respect to product formation and bacterial community structure during hydrogen production. METHODS AND RESULTS:Anaerobic microflora enriched from sludge com...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02939.x
更新日期:2006-08-01 00:00:00
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2002.01651.x
更新日期:2002-01-01 00:00:00
abstract:AIM:To investigate the immunoprotection of three recombinant proteins derived from the Vibrio harveyi outer membrane proteins (OMPs) OmpK, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and their fusion OmpK-GAPDH as vaccine candidates from vibriosis of large yellow croaker (Pseudosciaena crocea). METHODS:The ompK g...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03386.x
更新日期:2007-11-01 00:00:00
abstract:AIMS:To determine whether the beer-spoilage ability is an intrinsic character of Lactobacillus paracollinoides and identify a genetic marker for differentiating the beer-spoilage ability of strains belonging to this species. METHODS AND RESULTS:The ribotype of a nonspoilage strain, Lact. brevis ATCC8291, was found to ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02350.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The aims of this study were to: (1) estimate the effectiveness of ultraviolet radiation (UV) and sulfuric-acid based fertilizer (SA), at reducing levels of generic Escherichia coli in surface irrigation water and on produce and surface soil in open produce fields; and (2) describe the population dynamics of generi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.15011
更新日期:2021-01-22 00:00:00
abstract:AIMS:(i) To determine the aflatoxin B1 (AFB1 ) adsorption and desorption dynamics in the presence of Lactobacillus rhamnosus RC007 under simulated transit of AFB1 at each gastrointestinal tract (GIT-saliva, stomach and intestine) stage consecutively and then, separately, (ii) to study the ability of L. rhamnosus RC007 ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2019-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.04008.x
更新日期:2009-02-01 00:00:00
abstract::Techniques for the separation/concentration of micro-organisms from background food matrices can be applied to increase the speed of analysis and ease of isolation and detection of target micro-organisms. One recent example of such a technique is the immunomagnetic separation (IMS) procedure that has been used for the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:1997-08-01 00:00:00
abstract:AIMS:Fungal infection is still a life-threatening risk for the immunocompromised population such as AIDS patients and those who receive treatments with immunosuppressors and/or frequent administrations of wide-spectrum antibiotics, which inevitably lead to the drug resistance and unbalanced microflora populations. The ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02760.x
更新日期:2006-01-01 00:00:00
abstract:AIM:The aim of this study was to further characterize a bacterial culture (VUN 10,010) capable of benzo[a]pyrene cometabolism. METHODS AND RESULTS:The bacterial culture, previously characterized as a pure culture of Stenotrophomonas maltophilia (VUN 10,010), was found to also contain another bacterial species (Mycobac...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02087.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Molecular subtyping is commonly used in foodborne disease surveillance and microbial source tracking. There is a knowledge gap regarding the molecular ecology of foodborne pathogens in non-food-associated environments. The objective of this study was to isolate and subtype foodborne pathogens from pristine natural...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13657
更新日期:2018-02-01 00:00:00
abstract:AIM:A new medium, EC-Blue-10, containing chromogenic and fluorogenic substrates, KNO(3) and sodium pyruvate has been developed for the rapid simultaneous detection and enumeration of total coliforms and Escherichia coli in water. METHODS AND RESULTS:Two evaluations of EC-Blue-10 were carried out. Firstly, EC-Blue-10 w...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2007.03627.x
更新日期:2008-04-01 00:00:00
abstract:AIMS:To develop and evaluate primer sets targeted to the dissimilatory sulfite reductase gene (dsrA) for use in quantitative real-time PCR detection of sulfate-reducing bacteria (SRB) in stored swine manure. METHODS AND RESULTS:Degenerate primer sets were developed to detect SRB in stored swine manure. These were comp...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03900.x
更新日期:2008-12-01 00:00:00
abstract::Members of the genus Nocardiopsis are an ecologically versatile and biotechnologically important group of Actinomycetes. Most of the isolates are halotolerant or halophilic and they prevail in soils, marine environments or hypersaline locations. To aid their survival under these conditions, they mainly produce extremo...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.04964.x
更新日期:2011-04-01 00:00:00
abstract:AIMS:To investigate the effect of diet on the survival of Salmonella in the bovine abomasum. METHODS AND RESULTS:Five fistulated cows were randomly assigned to one of five diets denoted as: (i) 100% grass, (ii) grass + 5·3 kg DM concentrate, (iii) 100% grass silage, (iv) 100% hay and (v) maize/grass silage plus concen...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04836.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:To investigate low molecular weight compounds produced in vitro by Lysobacter capsici AZ78 and their toxic activity against sporangia of plant pathogenic oomycetes. METHODS AND RESULTS:Assays carried out in vitro showed that L. capsici AZ78 drastically inhibits the growth of plant pathogenic oomycetes. Accordingl...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12611
更新日期:2014-10-01 00:00:00
abstract:AIM:To assess Pediococcus acidilactici as a dietary supplement for on-growing red tilapia (Oreochromis niloticus). METHODS AND RESULTS:Tilapia were fed either a control diet or control diet supplemented with Ped. acidilactici at 10(7) CFU g(-1) for 32 days. Ped. acidilactici colonized the intestinal tract and signific...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04713.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:Biofilms in water distribution systems represent a far more significant reservoir of micro-organisms than the water phase. Biofilms are (i) resistant to disinfectants, (ii) nuclei for microbial regrowth, (iii) a refuge for pathogens, (iv) accompanied by taste and odour problems, and (v) corrode surfaces. The effec...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01974.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To evaluate the phenotypic expression of auto-aggregation, adhesion to mucin and biofilm formation of lactobacilli isolated from human vagina and the presence of related genes. METHODS AND RESULTS:Seven different strains of three Lactobacillus species (Lactobacillus gasseri, Lactobacillus rhamnosus and Lactobacil...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12642
更新日期:2014-12-01 00:00:00
abstract:AIMS:The transfer of tetO gene conferring resistance to tetracycline was studied between Campylobacter jejuni strains, in the digestive tract of chickens. METHODS AND RESULTS:In vitro conjugation experiments were first performed in order to select donor/recipient couples for further in vivo assay. Then, chickens were ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02306.x
更新日期:2004-01-01 00:00:00
abstract:AIM:Evaluation of the thermal and physical conditions for inactivation of adenovirus (AdV), porcine sapelovirus 1 (PSV1) and the economically important viruses porcine epidemic diarrhoea virus (PEDV) and porcine circovirus 2 (PCV2) in the production of spray-dried porcine plasma (SDPP). METHODS AND RESULTS:Citrate-tre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14235
更新日期:2019-06-01 00:00:00
abstract:AIMS:The aims of present study were to evaluate the abundances, genetic diversity of total and microcystin-producing Microcystis over temporal and spatial scales, and to investigate relationships among Microcystis and water parameters in Tai and Yang-cheng lakes. METHODS AND RESULTS:Abundances of total and microcystin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12456
更新日期:2014-06-01 00:00:00