Abstract:
AIMS:To determine the effects of water activity (a(W); 0.995-0.90), temperature (5, 18, 25 and 30 degrees C), time of incubation (7-35 days) and their interactions on tenuazonic acid (TA) production on 2% soybean-based agar by two Alternaria alternata strains isolated from soybean in Argentina. METHODS AND RESULTS:TA production by two isolates of A. alternata was examined under interacting conditions of a(W), temperature and time of incubation on 2% soybean-based agar. Maximum TA production was obtained for both strains at 0.98 a(W), but at 30 and 25 degrees C for the strains for RC 21 and RC 39, respectively. The toxin concentration varied considerably depending on a(W), temperature, incubation time and strain interactions. TA was produced over the temperature range from 5 to 30 degrees C and a(W) range from 0.92 to 0.995, however at 5 and 18 degrees C little TA was produced at a(W) below 0.94. Contour maps were developed from these data to identify areas where conditions indicate a significant risk for TA accumulation. CONCLUSIONS:The optimum and marginal conditions for TA production by A. alternata on soybean-based agar were identified. The results indicated that TA production by A. alternata is favoured by different temperatures in different strains. SIGNIFICANCE AND IMPACT OF THE STUDY:Data obtained provide very useful information for predicting the possible risk factors for TA contamination of soybean as the a(W) and temperature range used in this study simulate those occurring during grain ripening. The knowledge of TA production under marginal or sub-optimal temperature and a(W) conditions for growth are relevant as improper storage conditions accompanied by elevated temperature and moisture content in the grain can favour further mycotoxin production and lead to reduction in grain quality.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Oviedo MS,Ramirez ML,Barros GG,Chulze SNdoi
10.1111/j.1365-2672.2009.04301.xsubject
Has Abstractpub_date
2009-10-01 00:00:00pages
1186-92issue
4eissn
1364-5072issn
1365-2672pii
JAM4301journal_volume
107pub_type
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