A 210-min solid phase cytometry test for the enumeration of Escherichia coli in drinking water.

Abstract:

:A 210-min-test for the enumeration of Escherichia coli in drinking water is described, based on solid phase cytometry (SPC) and a two-step enzymatic procedure for fluorescence labelling of single cells and small microcolonies. The test involves membrane filtration through a 25-mm black polyester filter, induction of beta-glucuronidase in the retained target cells, fluorescence labelling with fluorescein-di-beta-Dglucuronide as an enzyme substrate and laser scanning of the membrane filter. Scan results can be confirmed on-line by epifluorescence microscopy. Application to 149 naturally contaminated and uncontaminated well, tap, out-of-pump centre (distribution), surface and sewage-spiked water samples indicated > or =90% agreement and equivalence with plate count methods, including Chromocult Coliform agar and m FC agar. In 5.4% of all samples examined, SPC detected between 1 and 11 E. coli per 100 ml, while the two plate methods yielded negative results. Cases of a negative SPC result but a positive E. coli count on both reference media were not observed. This test would primarily be useful for 'emergency' monitoring of drinking water when rapid results are crucial.

journal_name

J Appl Microbiol

authors

Van Poucke SO,Nelis HJ

doi

10.1046/j.1365-2672.2000.01125.x

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

390-6

issue

3

eissn

1364-5072

issn

1365-2672

journal_volume

89

pub_type

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