Heterologous expression of enterocin AS-48 in several strains of lactic acid bacteria.

Abstract:

AIMS:Enterococcus faecalis produces a cationic and circular enterocin, AS-48, of 7149 Da, the genetic determinants of which are located within the pMB2 plasmid. We have compared enterocin AS-48 production by different enterococci species with that of other 'safe' lactic acid bacteris (LAB) (GRAS status) and looked into the subsequent application of this enterocin in food production. METHODS AND RESULTS:In an effort to exploit this system for the heterologous expression of enterocin AS-48, a number of vectors containing the as-48 cluster were constructed and used to transform several LAB strains (genera Enterococcus, Lactococcus and Lactobacillus) CONCLUSION:Heterologous production of enterocin AS-48 failed when bacteria other than those belonging to the genus Enterococcus were used as hosts, although expression of a partial level of resistance against AS-48 were always detected, ruling out the possibility of a lack of recognition of the enterococcal promoters. SIGNIFICANCE AND IMPACT OF THE STUDY:Our results reveal the special capacity of species from the genus Enterococcus to produce AS-48, an enterocin that requires a post-transcriptional modification to generate a circular peptide with a wide range of inhibitory activity against pathogenic and spoilage bacteria. Preliminary experiments in foodstuffs using nonvirulent enterococci with interesting functional properties reveal the possibility of a biotechnological application of these transformants.

journal_name

J Appl Microbiol

authors

Fernández M,Martínez-Bueno M,Martín MC,Valdivia E,Maqueda M

doi

10.1111/j.1365-2672.2006.03194.x

subject

Has Abstract

pub_date

2007-05-01 00:00:00

pages

1350-61

issue

5

eissn

1364-5072

issn

1365-2672

pii

JAM3194

journal_volume

102

pub_type

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