Molecular diagnostics on the toxigenic potential of Fusarium spp. plant pathogens.

Abstract:

AIMS:We propose and test an efficient and rapid protocol for the detection of toxigenic Fusarium isolates producing three main types of Fusarium-associated mycotoxins (fumonisins, trichothecenes and zearelanone). METHODS AND RESULTS:The novel approach utilizes partially multiplexed markers based on genes essential for mycotoxin biosynthesis (fumonisin--fum6, fum8; trichothecenes--tri5, tri6; zearalenone, zea2) in Fusarium spp. The protocol has been verified by screening a collection of 96 isolates representing diverse species of filamentous fungi. Each Fusarium isolate was taxonomically identified through both molecular and morphological techniques. The results demonstrate a reliable detection of toxigenic potential for trichothecenes (sensitivity 100%, specificity 95%), zearalenone (sensitivity 100%, specificity 100%) and fumonisins (sensitivity 94%, specificity 88%). Both presence and identity of toxin biosynthetic genes were further confirmed by direct sequencing of amplification products. CONCLUSIONS:The cross-species-specific PCR markers for key biosynthetic genes provide a sensitive detection of toxigenic fungal isolates, contaminating biological material derived from agricultural fields. SIGNIFICANCE AND IMPACT OF THE STUDY:The conducted study shows that a PCR-based assay of biosynthetic genes is a reliable, cost-effective, early warning system against Fusarium contamination. Its future use as a high-throughput detection strategy complementing chemical assays enables effective targeted application of crop protection products.

journal_name

J Appl Microbiol

authors

Dawidziuk A,Koczyk G,Popiel D,Kaczmarek J,Buśko M

doi

10.1111/jam.12488

subject

Has Abstract

pub_date

2014-06-01 00:00:00

pages

1607-20

issue

6

eissn

1364-5072

issn

1365-2672

journal_volume

116

pub_type

杂志文章
  • Gut microbiota of animals living in polluted environments are a potential resource of anticancer molecules.

    abstract::Cancer is a prominent cause of morbidity and mortality worldwide, in spite of advances in therapeutic interventions and supportive care. In 2018 alone, there were 18·1 million new cancer cases and 9·6 million deaths indicating the need for novel anticancer agents. Plant-based products have often been linked with prote...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.14981

    authors: Soopramanien M,Khan NA,Siddiqui R

    更新日期:2020-12-23 00:00:00

  • Evaluating the role of total organic carbon in predicting the treatment efficacy of biosand filters for the removal of Vibrio cholerae in drinking water during startup.

    abstract:AIMS:In biosand filters (BSF), treatment is largely driven by the development of a biolayer (schmutzdecke) which establishes itself during the startup phase. In this study, the effect of changing influent total organic carbon (TOC) loading on the removal efficiency of Vibrio cholerae in laboratory-operated BSFs was qua...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.13909

    authors: Danley-Thomson AA,Huang EC,Worley-Morse T,Gunsch CK

    更新日期:2018-09-01 00:00:00

  • Common occurrence of Gallid herpesvirus-2 with reticuloendotheliosis virus in chickens caused by possible contamination of vaccine stocks.

    abstract:AIMS:The aim of this study was to investigate the common occurrence of reticuloendotheliosis virus (REV) among Gallid herpesvirus 2 (GaHV-2) infected chickens. The possible cause of this co-occurrence may be linked to contaminated vaccine stocks, which were also examined. METHODS AND RESULTS:The study was conducted on...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12734

    authors: Woźniakowski G,Mamczur A,Samorek-Salamonowicz E

    更新日期:2015-04-01 00:00:00

  • Solar disinfection of drinking water contained in transparent plastic bottles: characterizing the bacterial inactivation process.

    abstract::A series of experiments is reported to identify and characterize the inactivation process in operation when drinking water, heavily contaminated with a Kenyan isolate of Escherichia coli, is stored in transparent plastic bottles that are then exposed to sunlight. The roles of optical and thermal inactivation mechanism...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00455.x

    authors: McGuigan KG,Joyce TM,Conroy RM,Gillespie JB,Elmore-Meegan M

    更新日期:1998-06-01 00:00:00

  • Nutritional requirements and simplified cultivation medium to study growth and energetics of a sourdough lactic acid bacterium Lactobacillus fermentum Ogi E1 during heterolactic fermentation of starch.

    abstract:AIMS:Nutritional requirements of Lactobacillus fermentum Ogi E1 were studied in order to define a simplified fermentation medium. METHODS AND RESULTS:When grown with MRS-medium in 2l bioreactors, a biphasic pattern of growth and metabolite production was observed. Study of nutritional requirements resulted in a simpli...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01272.x

    authors: Calderon M,Loiseau G,Guyot JP

    更新日期:2001-04-01 00:00:00

  • Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA-894.

    abstract:AIMS:To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. METHODS AND RESULTS:A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were pur...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2012.05263.x

    authors: Wang L,Hu X,Tao G,Wang X

    更新日期:2012-05-01 00:00:00

  • A rapid method for the evaluation of both extrinsic and intrinsic contamination and resulting spoilage of water-in-oil emulsions.

    abstract:AIMS:To develop a method for studying the microbial spoilage of water-in-oil emulsions and to use this to investigate (i) the intrinsic stability of water-in-oil formulations and (ii) Pseudomonas aeruginosa SP1-induced spoilage of a proprietary emulsion. METHODS AND RESULTS:Aliquots of test emulsion were placed into w...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2004.02239.x

    authors: O'May GA,Allison DG,Gilbert P

    更新日期:2004-01-01 00:00:00

  • Antimicrobial and disinfectant resistance of Escherichia coli isolated from giant pandas.

    abstract:AIMS:The study aims to demonstrate the antimicrobial and disinfectant resistance phenotypes and genotypes of Escherichia coli isolates obtained from giant pandas (Ailuropoda melanoleuca). METHODS AND RESULTS:Antimicrobial testing was performed according to the standard disk diffusion method. The minimal inhibitory con...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12820

    authors: Guo L,Long M,Huang Y,Wu G,Deng W,Yang X,Li B,Meng Y,Cheng L,Fan L,Zhang H,Zou L

    更新日期:2015-07-01 00:00:00

  • The application of viable count procedures for measuring viable cells in the various marine environments.

    abstract:AIMS:To investigate whether the use of direct viable count (DVC), quantitative viable count (qDVC), colony-forming units and the contribution of capsule-bearing bacteria to the total number of bacteria and esterase-active bacteria could be used to clearly differentiate viable cells in various trophic status of seawater...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04411.x

    authors: Altug G,Cardak M,Ciftci PS,Gurun S

    更新日期:2010-01-01 00:00:00

  • Modelling salinity inhibition effects during biodegradation of perchlorate.

    abstract:AIMS:To determine the mathematical kinetic rates and mechanisms of acclimated perchlorate (ClO)-reducing microbial cultures by incorporating a term to relate the inhibitory effect of high salinity during biological reduction of concentrated perchlorate solutions. METHODS AND RESULTS:Salt toxicity associated with the b...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2006.02950.x

    authors: Park C,Marchand EA

    更新日期:2006-07-01 00:00:00

  • Characterization and validation of a chemiluminescent assay based on a 1,2-dioxetane for rapid detection of enterococci in contaminated water and comparison with standard methods and qPCR.

    abstract:AIMS:To evaluate the potential for using a novel chemiluminescence-based enzyme assay for rapid detection of enterococci in water contaminated with faecal waste. METHODS AND RESULTS:The novel assay (EntLight) was based on the enzymatic hydrolysis of the chemiluminescent 1,2-dioxetane [(4-methoxy-4(3-β-d-glucoside-4-ch...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.05051.x

    authors: Bukh AS,Roslev P

    更新日期:2011-08-01 00:00:00

  • Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.

    abstract:AIMS:To assess the applicability of the 16S-23S rDNA internal spacer regions (ISR) as targets for PCR detection of Azospirillum ssp. and the phytostimulatory plant growth-promoting rhizobacteria seed inoculant Azospirillum lipoferum CRT1 in soil. METHODS AND RESULTS:Primer sets were designed after sequence analysis of...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2009.04393.x

    authors: Baudoin E,Couillerot O,Spaepen S,Moënne-Loccoz Y,Nazaret S

    更新日期:2010-01-01 00:00:00

  • Analysis of environmental Escherichia coli isolates for virulence genes using the TaqMan PCR system.

    abstract:AIMS:To assess the presence of virulence genes in environmental and foodborne Escherichia coli isolates using the TaqMan PCR system. METHODS AND RESULTS:Three TaqMan pathogen detection kits called O157:H7, StxI and StxII were used to investigate the presence of virulence genes in Escherichia coli isolates. All 54 food...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.02023.x

    authors: Davis KC,Nakatsu CH,Turco R,Weagant SD,Bhunia AK

    更新日期:2003-01-01 00:00:00

  • Mutagens manufactured in fungal culture may affect DNA/RNA of producing fungi.

    abstract::Self-produced mutagens in culture by fungi may affect DNA analysis of the same fungi. This has not been considered previously. Many fungi produce numerous mutagenic secondary metabolites (SM) in culture. There is a paradox of growing fungi in media to produce representative DNA which also support mutagenic SM. This is...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2672.2008.04024.x

    authors: Paterson RR,Lima N

    更新日期:2009-04-01 00:00:00

  • The effects of cleaning and disinfection in reducing Salmonella contamination in a laboratory model kitchen.

    abstract:AIMS:To establish a laboratory model to compare the effectiveness of detergent-based disinfection procedures for reducing cross-contamination risks during handling of contaminated chicken. METHODS AND RESULTS:During handling of chickens, artificially contaminated with Salmonella enteritidis PT4, the organism was widel...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2003.02127.x

    authors: Barker J,Naeeni M,Bloomfield SF

    更新日期:2003-01-01 00:00:00

  • In vitro potential antioxidant activity of indigenous yeasts isolated from virgin olive oil.

    abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14520

    authors: Ciafardini G,Zullo BA

    更新日期:2020-03-01 00:00:00

  • Activities of muscadine grape skin and quercetin against Helicobacter pylori infection in mice.

    abstract:AIMS:To explore the preventative potential of muscadine grape skin (MGS) and the single flavonoid, quercetin, as an alternative means for ameliorating Helicobacter pylori infection and/or the H. pylori-induced inflammatory response in mice. METHODS AND RESULTS:The antimicrobial and anti-inflammatory properties of MGS ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04870.x

    authors: Brown JC,Wang J,Kasman L,Jiang X,Haley-Zitlin V

    更新日期:2011-01-01 00:00:00

  • A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans.

    abstract:AIMS:This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. METHODS AND RESULTS:The effect of Candida infection on host cell viability was evaluated by the microscopy of t...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12817

    authors: Boros-Majewska J,Turczyk Ł,Wei X,Milewski S,Williams DW

    更新日期:2015-07-01 00:00:00

  • Characterization and mechanism of action of cerein 7, a bacteriocin produced by Bacillus cereus Bc7.

    abstract::Cerein 7 is a peptidic antibiotic produced by Bacillus cereus Bc7 (CECT 5148) at the end of exponential growth but before sporulation onset. Cerein 7 has a broad spectrum of antibacterial activity against Gram-positive bacteria, but it is inactive against Gram-negative bacteria. The sequence of its amino-terminal end ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.01123.x

    authors: Oscáriz JC,Pisabarro AG

    更新日期:2000-08-01 00:00:00

  • Evaluation of real-time PCR methods for quantification of Acanthamoeba in anthropogenic water and biofilms.

    abstract:AIMS:To assess two real-time PCR methods (the Riviere and Qvarnstrom assays) for environmental Acanthamoeba. METHODS AND RESULTS:DNA extracted from Acanthamoeba castellanii taken from water and biofilms of cooling towers was analysed by the Riviere and Qvarnstrom assays. To quantify environmental Acanthamoeba, the cal...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2010.04708.x

    authors: Chang CW,Wu YC,Ming KW

    更新日期:2010-09-01 00:00:00

  • Kinetics of carbon sharing in a bacterial consortium revealed by combining stable isotope probing with fluorescence-activated cell sorting.

    abstract:AIMS:  To determine the kinetics of substrate fluxes in a microbial community in order to elucidate the roles of the community members. METHODS AND RESULTS:  The kinetics of substrate sharing in a bacterial consortium were measured by a new analytical approach combining immunostaining, stable isotope probing and fluor...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2011.04964.x

    authors: Pawelczyk S,Bumann D,Abraham WR

    更新日期:2011-04-01 00:00:00

  • Shoe soles as a potential vector for pathogen transmission: a systematic review.

    abstract::Shoe soles are possible vectors for infectious diseases. Although studies have been performed to assess the prevalence of infectious pathogens on shoe soles and decontamination techniques, no systematic review has ever occurred. The aim of this study was to perform a systematic review of the literature to determine th...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/jam.13250

    authors: Rashid T,VonVille HM,Hasan I,Garey KW

    更新日期:2016-11-01 00:00:00

  • Culture conditions influencing phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle.

    abstract:AIMS:The effects of pH, temperature, phytate, glucose, phosphate and surfactants on the phytase production of Mitsuokella jalaludinii, a new bacterial species from the rumen of cattle, were evaluated. METHOD AND RESULTS:By using batch fermentation, it was found that phytase production of M. jalaludinii was induced by ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2002.01727.x

    authors: Lan GQ,Abdullah N,Jalaludin S,Ho YW

    更新日期:2002-01-01 00:00:00

  • Production of conjugated linoleic acid by dairy starter cultures.

    abstract::Nineteen different strains of lactobacilli, lactococci, streptococci and propionibacteria commonly used as dairy starter cultures were tested for their ability to produce conjugated linoleic acid (CLA) from free linoleic acid in vitro. Two strains of Propionibacterium freudenreichii ssp. freudenreichii and one strain ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1998.00481.x

    authors: Jiang J,Björck L,Fondén R

    更新日期:1998-07-01 00:00:00

  • Drought degree constrains the beneficial effects of a fungal endophyte on Atractylodes lancea.

    abstract:AIMS:Plants, fungal endophytes (FEs) and the changing environment interact with each other forming an interlaced network. This study evaluates nonadditive and interactive effects of the FE Acremonium strictum and drought treatment on Atractylodes lancea plantlets. METHODS AND RESULTS:By applying FEs (meristem cultures...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.12615

    authors: Yang T,Ma S,Dai CC

    更新日期:2014-11-01 00:00:00

  • Low temperature bioremediation of oil-contaminated soil using biostimulation and bioaugmentation with a Pseudomonas sp. from maritime Antarctica.

    abstract:AIMS:To identify native Antarctic bacteria capable of oil degradation at low temperatures. METHODS AND RESULTS:Oil contaminated and pristine soils from Signy Island (South Orkney Islands, Antarctica) were examined for bacteria capable of oil degradation at low temperatures. Of the 300 isolates cultured, Pseudomonas st...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2005.02678.x

    authors: Stallwood B,Shears J,Williams PA,Hughes KA

    更新日期:2005-01-01 00:00:00

  • Viability and contribution to proteolysis of an adjunct culture of Lactobacillus plantarum in two model cheese systems: cheddar cheese-type and soft-cheese type.

    abstract:AIMS:The influence of the cheese-making process, ripening conditions and primary starter on the viability and proteolytic activity of an adjunct culture of Lactobacillus plantarum I91 was assessed in two miniature cheese models, representative of Cremoso Argentino and Cheddar cheeses. METHODS AND RESULTS:Cheeses with ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2008.03813.x

    authors: Milesi MM,McSweeney PL,Hynes ER

    更新日期:2008-09-01 00:00:00

  • Functional analysis of superoxide dismutase of Salmonella typhimurium in serum resistance and biofilm formation.

    abstract:AIMS:Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium). METHODS AND RESULTS:A deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/jam.14044

    authors: Wang Y,Yi L,Zhang J,Sun L,Wen W,Zhang C,Wang S

    更新日期:2018-11-01 00:00:00

  • Purification and characterization of a bacteriocin-like compound (Lichenin) produced anaerobically by Bacillus licheniformis isolated from water buffalo.

    abstract:AIMS:To characterize a bacteriocin-like factor from Bacillus licheniformis 26 L-10/3RA isolated from buffalo rumen. METHODS AND RESULTS:The culture supernatant exhibited the antibacterial activity against a number of indicator organisms in a cut-well agar assay under anaerobic conditions. The inhibitory component was ...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2001.01429.x

    authors: Pattnaik P,Kaushik JK,Grover S,Batish VK

    更新日期:2001-10-01 00:00:00

  • Persistence of Shiga toxin-producing Escherichia coli O26 in various manure-amended soil types.

    abstract:AIMS:To evaluate the behaviour of Shiga toxin-producing Escherichia coli (STEC) O26 strains inoculated in manure-amended soils under in vitro conditions. METHODS AND RESULTS:Four green fluorescent protein (GFP)-labelled STEC O26 strains were inoculated in duplicate (at 10(6) CFU g(-1)) in three different manure-amende...

    journal_title:Journal of applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2672.2007.03532.x

    authors: Fremaux B,Prigent-Combaret C,Delignette-Muller ML,Mallen B,Dothal M,Gleizal A,Vernozy-Rozand C

    更新日期:2008-01-01 00:00:00