Abstract:
AIMS:To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. METHODS AND RESULTS:One hundred fifty strains belonging to Lactobacillus spp. and Weissella spp., plus eight type strains, one for each species, and two unidentified isolates, were characterized by randomly amplified polymorphic DNA (RAPD) and SDS-PAGE of cell-wall proteins. The RAPD analysis separated the eight type strains but did not always assign all the strains of a species to the same group, while SDS-PAGE cell-wall protein profiles were species-specific. Frequently, strains isolated from sourdoughs of the same geographical origin or produced by similar raw material/process parameters showed similar RAPD and/or cell-wall profiles. CONCLUSIONS:The combined use of the RAPD and cell-wall protein analysis represents a useful tool to classify large adventitious microbial populations and to discriminate the diversity of the strains. SIGNIFICANCE AND IMPACT OF THE STUDY:This study represents a typing of a large collection of flour/sourdough LAB and provides evidence of the advantage of using two independent methods in the classification and traceability of microorganisms.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Corsetti A,De Angelis M,Dellaglio F,Paparella A,Fox PF,Settanni L,Gobbetti Mdoi
10.1046/j.1365-2672.2003.01874.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
641-54issue
4eissn
1364-5072issn
1365-2672pii
1874journal_volume
94pub_type
杂志文章abstract::Thirty-eight Lactococcus strains, isolated from raw milk produced in two dairy areas in Normandy, were identified at the phenotypic level. Only Lactococcus lactis strains with the lactis phenotype were found in the milk samples. Most strains fermented lactose (97%) and showed proteinase activity (76%). Isolates were c...
journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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abstract::Cerein 7 is a peptidic antibiotic produced by Bacillus cereus Bc7 (CECT 5148) at the end of exponential growth but before sporulation onset. Cerein 7 has a broad spectrum of antibacterial activity against Gram-positive bacteria, but it is inactive against Gram-negative bacteria. The sequence of its amino-terminal end ...
journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2000.01123.x
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abstract:AIMS:The aims of this study were to: (1) estimate the effectiveness of ultraviolet radiation (UV) and sulfuric-acid based fertilizer (SA), at reducing levels of generic Escherichia coli in surface irrigation water and on produce and surface soil in open produce fields; and (2) describe the population dynamics of generi...
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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abstract:AIM:Identification and enumeration of foodborne pathogens in food stuffs are valuable concerns. In the present study, starch-blood-egg yolk-polymyxin B-trimethoprim-ceftazidime (SBYPTC) agar was established to isolate and specify the number of Bacillus cereus in food products. METHODS AND RESULTS:The effectiveness of ...
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journal_title:Journal of applied microbiology
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doi:10.1046/j.1365-2672.2001.01482.x
更新日期:2001-12-01 00:00:00
abstract:AIMS:A relative quantification system (RQ-PCR) was used to monitor the correlations between the activity of the nor-1 (=aflD) gene of Aspergillus flavus using real-time PCR in relation to phenotypic aflatoxin B(1) (AFB(1) ) production and populations of A. flavus in stored peanuts at three water activity levels (a(w) ,...
journal_title:Journal of applied microbiology
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更新日期:2020-09-11 00:00:00