Abstract:
AIMS:The objective of this study was to investigate the effect of temperature (5-25°C) on epiphytic lactic acid bacteria (LAB) populations during 60 days of fermentation of whole-plant corn silage. METHODS AND RESULTS:Vacuum bag mini-silos of chopped whole-plant corn were incubated at five different temperatures (5, 10, 15, 20 and 25°C), according to a completely randomized design with four repetitions. The silos were opened and sampled on day 0, 1, 2, 3, 7, 28 and 60. At 20 and 25°C, Lactobacillus plantarum- and Pediococcus pentosaceus-related operational taxonomic units (OTU) dominated the fermentation within 1 day. After 7 days, the OTU related to the heterofermentative species Lactobacillus buchneri began to appear and it eventually dominated silages incubated at these temperatures. Population dynamic of LAB at 5 and 10°C was different. At these temperatures, Leuconostoc citreum OTU was identified at the beginning of the fermentation. Thereafter, Lactobacillus sakei- and Lactobacillus curvatus-related OTU appeared and quickly prevailed. Corn silage at 15°C acted as a transition between 20-25°C and 5-10°C, in terms of LAB diversity and succession. CONCLUSION:The conditions of silage incubation temperature affect species diversity of LAB population with notable difference along the temperature gradient. Colder temperature conditions (5 and 10°C) have led to the identification of LAB species never observed in corn silage. SIGNIFICANCE AND IMPACT OF THE STUDY:This study demonstrated the impact of temperature gradient on the diversity and some important population shift of lactic acid bacteria communities during fermentation of corn silage.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Zhou Y,Drouin P,Lafrenière Cdoi
10.1111/jam.13198subject
Has Abstractpub_date
2016-09-01 00:00:00pages
657-71issue
3eissn
1364-5072issn
1365-2672journal_volume
121pub_type
杂志文章abstract:AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02472.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To develop and evaluate cross-priming amplification (CPA) combined with immuno-blotting for the detection of coagulase-positive Staphylococci including Staphylococcus aureus. METHODS AND RESULTS:Twenty-four sets of cross and detection primers were designed according to four sequences of coagulase gene in Staph. a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12836
更新日期:2015-07-01 00:00:00
abstract:AIMS: The effect of refrigeration on the seafood-borne pathogen Vibrio vulnificus was investigated in terms of genotype selection and persistence in refrigerated oysters. METHODS AND RESULTS: Naturally occurring numbers of V. vulnificus in oysters from two different locations were compared during a 2-week period und...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05306.x
更新日期:2012-07-01 00:00:00
abstract:AIMS:Recombinant Saccharomyces cerevisiae strains harbouring different levels of xylulokinase (XK) activity and effects of XK activity on utilization of xylulose were studied in batch and fed-batch cultures. METHODS AND RESULTS:The cloned xylulokinase gene (XKS1) from S. cerevisiae was expressed under the control of t...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.02055.x
更新日期:2003-01-01 00:00:00
abstract::A rhamnolipid-producing strain of Pseudomonas aeruginosa GL1 was isolated from a bacterial community growing on a mixture of polycyclic aromatic hydrocarbons (PAH) as sole carbon source. Strain GL1 did not grow on PAH but grew on known degradation metabolites of phenanthrene (O-phthalic acid) and of naphthalene (salic...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1998.00412.x
更新日期:1998-05-01 00:00:00
abstract:AIMS:The aim of this study was to develop and optimize a novel method that combines ethidium bromide monoazide (EMA) staining with real-time PCR for the detection of viable Escherichia coli O157:H7 in ground beef. EMA can penetrate dead cells and bind to intracellular DNA, preventing its amplification via PCR. METHODS...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04358.x
更新日期:2009-11-01 00:00:00
abstract:AIMS:As part of a field-based study of the distribution and persistence of Salmonella infection on commercial egg-laying farms, sampling was carried out on one or more occasions in egg-packing areas of 12 farms infected with Salm. Enteritidis. METHODS AND RESULTS:Salmonellas were isolated by cultural methods. Contamin...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01817.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To determine the differences in competitive fitness among Escherichia coli strains with different plasmid profiles when grown in suspension with commensal faecal bacteria from growing swine fed chlortetracycline-supplemented or unsupplemented diets. METHODS AND RESULTS:Five multiple drug-resistant (MDR) E. coli s...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05365.x
更新日期:2012-09-01 00:00:00
abstract:AIMS:To investigate the in vitro antibacterial activity and antifungal mode of action of flocculosin, a cellobiose lipid produced by Pseudozyma flocculosa. METHODS AND RESULTS:When tested against clinical bacterial isolates, the compound was particularly active against Gram-positive bacteria and its effect was not mit...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04280.x
更新日期:2009-09-01 00:00:00
abstract:AIMS:To determine the occurrence of Escherichia coli harbouring virulence markers of shiga- or entero-toxins and resistance to antimicrobials in surface waters. METHODS AND RESULTS:Surface water samples were collected at six locations of the river Gomti. E. coli isolates (n = 90) were characterized for their pathogeni...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2008.03879.x
更新日期:2008-12-01 00:00:00
abstract:AIMS:To study the accumulation of the bacterial living cells (LC) and dead cells (DC) in a mixed-species biofilm developed in a 3 l biotrickling filter (BTF) challenged with toluene. METHODS AND RESULTS:The bacterial LC and DC within the biofilm developed on polypropylene Pall rings in a toluene-degrading BTF were enu...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2003.01914.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To monitor seasonal variations in the microbiology of maize silage and to determine whether the risk of fungal spoilage varies during whole-year storage. METHODS AND RESULTS:A continuous survey of 20 maize silage stacks was conducted over a period from three to 11 months after ensiling. Filamentous fungi, yeasts ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04729.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:Three broadly used typing methods were employed in order to assess and compare the identification and classification of environmental Pseudomonas strains. The reproducibility, typeability and discriminatory power of the methods were also compared to evaluate their application. Finally, the potential impact on publ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02691.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02837.x
更新日期:2006-04-01 00:00:00
abstract:AIM:To develop a faster and easier in vivo method to screen compounds for anti-oxidant activity using a microbial system. METHODS AND RESULTS:Bacterial redox sensor-based assay systems were applied. The activities of SoxR and OxyR, the bacterial redox sensors, were monitored to probe the intracellular redox status thr...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04514.x
更新日期:2010-04-01 00:00:00
abstract:AIMS:The biopesticide effect of four green composts against fusarium wilt in melon plants and the effect of soil quality in soils amended with composts were assayed. METHODS AND RESULTS:The composts consisted of pruning wastes, with or without addition of coffee wastes (3/1 and 4/1, dry wt/dry wt) or urea (1000/1, dry...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02508.x
更新日期:2005-01-01 00:00:00
abstract:AIM:Evaluation of the genetic variability of stolbur phytoplasma infecting grapevines, bindweeds and vegetables, collected in different central and southern Italian regions. MATERIALS AND RESULTS:Phytoplasma isolates belonging to stolbur subgroup 16SrXII-A were subjected to molecular characterization by polymerase cha...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2010.04835.x
更新日期:2010-12-01 00:00:00
abstract::The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01189.x
更新日期:2000-11-01 00:00:00
abstract:AIM:To study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous Enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. METHODS AND RESULTS:Six Enterococcus faecalis strains were paired with 15 other microbial isolates to form mix...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03187.x
更新日期:2007-05-01 00:00:00
abstract:AIMS:To investigate the occurrence and extent of Saccharomyces cerevisiae and Oenococcus oeni interactions. METHODS AND RESULTS:Interactions between S. cerevisiae and O. oeni were investigated by double-layer and well-plate assays showing the occurrence of specific interactions for each yeast-malolactic bacteria (MLB)...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2005.02579.x
更新日期:2005-01-01 00:00:00
abstract:AIM:To apply a quantitative reverse-transcription PCR (qRT-PCR) method to determine the total viable count (TVC) on meat samples. METHODS AND RESULTS:Using two sets of primers to target the ribonuclease-P (RNase P) RNA transcripts of Gram-positive and Gram-negative bacteria, standard curves were generated using the Li...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04636.x
更新日期:2010-07-01 00:00:00
abstract::Although there is great scientific and technological interest in examining the physiology and bioenergetics of microorganisms from extreme environments, difficulties encountered in their cultivation and lack of genetic systems hampers the investigation of these issues. As such, we have adapted methods for continuous c...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.1998.tb05290.x
更新日期:1998-12-01 00:00:00
abstract:AIMS:The chitinolytic activity of an actinomycete, isolated from a tropical acidic ferrasol (FAO) under cerrado (savanna) vegetation, is reported. METHODS AND RESULTS:Selection of the strain was based on spot inoculation on solid colloidal chitin medium. The use of chemotaxonomic, morphological and physiological proce...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01294.x
更新日期:2001-04-01 00:00:00
abstract:AIMS:The aim of the study was to check whether two flavanols ((-)-epigallocatechin gallate and (+)-catechin) can ameliorate oxidative stress (OS) accompanying and contributing to the lethal effects of heating (50°C) and freezing-thawing on the yeast Saccharomyces cerevisiae. METHODS AND RESULTS:The flavanols studied i...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14170
更新日期:2019-03-01 00:00:00
abstract:AIMS: The germination-arrest factor (GAF) produced by Pseudomonas fluorescens WH6, and identified as 4-formylaminooxyvinylglycine, specifically inhibits the germination of a wide range of grassy weeds. This study was undertaken to determine whether GAF has antimicrobial activity in addition to its inhibitory effects on...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2011.05098.x
更新日期:2011-10-01 00:00:00
abstract::Water-buffalo Mozzarella (WBM) cheese is one of the several 'pasta filata' or stretched curd cheeses that originated in southern Italy, traditionally manufactured from raw milk employing natural whey starter cultures. Lactose- and galactose-fermenting yeasts isolated from WBM were studied to evaluate their role in the...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00926.x
更新日期:2000-01-01 00:00:00
abstract:AIMS:To contribute to the understanding of Cytisus scoparius success at invading and establishing itself in Australia. METHODS AND RESULTS:Root-nodule bacteria isolated from C. scoparius, growing on five different sites and originally introduced to Australia, were compared with isolates from indigenous plants growing ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.02902.x
更新日期:2006-06-01 00:00:00
abstract:AIMS:The ability of 31 Lactobacillus plantarum strains to adhere to biological matrixes was evaluated, and the molecules involved in adherence were studied. METHODS AND RESULTS:Mucin, basement membrane proteins and Caco-2 cells were used in adhesion tests. These in vitro assays, together with a yeast agglutination tes...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03086.x
更新日期:2007-02-01 00:00:00
abstract:AIMS:Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium). METHODS AND RESULTS:A deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14044
更新日期:2018-11-01 00:00:00
abstract:AIMS:This study was performed to analyse the molecular characteristics of genes encoding for the major virulence factors in Bacillus anthracis vaccine strain 'Carbosap' compared with the wild B. anthracis strain, to evaluate the basis of attenuation. METHODS AND RESULTS:The molecular characteristics of the B. anthraci...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01660.x
更新日期:2002-01-01 00:00:00