Abstract:
AIMS:To provide molecular fingerprinting evidence of the contribution of wildlife vectors in the on-farm epidemiology of Salmonella Enteritidis infections. METHODS AND RESULTS:Salmonella Enteritidis strains were isolated from wildlife and from farm environment samples collected in 10 egg layer farms. Isolates were typed using plasmid profiling, XbaI-pulsed field gel electrophoresis and PstI-SphI ribotyping. In all 10 farms we were able to identify the same S. Enteritidis clones in wildlife vectors and farm environment. On several occasions the same clones were found before and after cleansing and disinfecting the farm premises. Also in some instances the same clones were present in mice samples, egg contents and spent hens. CONCLUSIONS:Definitive molecular evidence for the involvement of several wildlife species (mice, rats, flies, litter beetles and foxes) in the maintenance of S. Enteritidis infection on farms has been presented. Failures in biosecurity seriously compromise the control of this pathogen on laying farms. SIGNIFICANCE AND IMPACT OF THE STUDY:This paper reports on the use of molecular tools for the study of the epidemiology of S. Enteritidis. It gives useful information to be considered in control programmes for this organism on poultry farms.
journal_name
J Appl Microbioljournal_title
Journal of applied microbiologyauthors
Liebana E,Garcia-Migura L,Clouting C,Clifton-Hadley FA,Breslin M,Davies RHdoi
10.1046/j.1365-2672.2003.01924.xsubject
Has Abstractpub_date
2003-01-01 00:00:00pages
1024-9issue
6eissn
1364-5072issn
1365-2672pii
1924journal_volume
94pub_type
杂志文章abstract:AIMS:To clarify the effects of Lactobacillus gasseri CP2305 (CP2305) on quality of life and clinical symptoms and its functional mechanisms in patients with irritable bowel syndrome (IBS). METHODS AND RESULTS:After the patients were administered CP2305 daily for 4 weeks, the IBS-severity index score was significantly ...
journal_title:Journal of applied microbiology
pub_type: 临床试验,杂志文章
doi:10.1111/jam.13329
更新日期:2017-01-01 00:00:00
abstract:AIM:To screen a lactic acid bacteria (LAB) strain for good adaption in alfalfa ensiling and to evaluate its effects on alfalfa ensiling. METHODS AND RESULTS:Lactobacillus plantarum (LP) strain ZZU203 was selected due to its superior ability to acidify structural carbohydrate metabolite and alfalfa powder. Alfalfa was ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2020-08-01 00:00:00
abstract:AIMS:Mineral deficiencies cause several health problems in the world, especially for populations consuming cereal-based diets rich in the anti-nutrient phytate. Our aim was to characterize the phytate-degrading capacity of the yeast Pichia kudriavzevii TY13 and its secretion of phytase. METHODS AND RESULTS:The phytase...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12767
更新日期:2015-05-01 00:00:00
abstract:AIMS:Microbial induced calcium carbonate precipitation (MICP) is one of the bio-cementation methods for improving granular soils. This study evaluate the feasibility of obtaining a bacterial solution with high optical density and urease activity by an inexpensive corn steep liquor (CSL) medium in non-sterile conditions...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14866
更新日期:2020-10-07 00:00:00
abstract:AIMS:A method for rapid and simultaneous detection, identification and enumeration of specific micro-organisms using Peptide Nucleic Acid (PNA) probes is presented. METHODS AND RESULTS:The method is based on a membrane filtration technique. The membrane filter was incubated for a short period of time. The microcolonie...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01230.x
更新日期:2001-02-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the common occurrence of reticuloendotheliosis virus (REV) among Gallid herpesvirus 2 (GaHV-2) infected chickens. The possible cause of this co-occurrence may be linked to contaminated vaccine stocks, which were also examined. METHODS AND RESULTS:The study was conducted on...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12734
更新日期:2015-04-01 00:00:00
abstract:AIM:The aim of the present study was to investigate the in vitro antioxidant activity of yeast strains isolated from virgin olive oil. METHODS AND RESULTS:The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free-radical scavenging activity of single cultures of 24 yeast strains belonging to eight species isolated from virgin ol...
journal_title:Journal of applied microbiology
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更新日期:2020-03-01 00:00:00
abstract:AIMS:The aim of this study was to elucidate the effect of arginine on the growth, arginine metabolism and amino acid consumption profiles of Streptococcus thermophilus T1C2. METHODS AND RESULTS:The growth kinetics, intracellular pH, extracellular osmotic pressure, expression of key genes in the arginine metabolism pat...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13221
更新日期:2016-09-01 00:00:00
abstract:AIMS:The purpose of this work was to study the involvement of micro-organisms, which develop together with Botrytis cinerea on grapes, in the SO2 binding power of musts. METHODS AND RESULTS:Yeasts and bacteria were involved. Most bacteria were acetic acid bacteria, mainly of the Gluconobacter genus. Unlike oxidative y...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2001.01200.x
更新日期:2001-01-01 00:00:00
abstract:AIMS:To develop qPCR broad-spectrum primers combined with a Nocardia genus-specific probe for the identification of a broad spectrum of Nocardia spp. and to analyse the effects of using this developed primer and probe set on the ability to quantify Nocardia spp. in mixed DNA. METHODS AND RESULTS:The consequences of us...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13311
更新日期:2017-01-01 00:00:00
abstract:AIMS:Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia pastoris. And to obtain a purified and characterized recombinant laccase to analyse the biotechnological application potential. METHODS AND RESULTS:Lac I was cloned an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13720
更新日期:2018-06-01 00:00:00
abstract:AIMS:Investigation of waste pulps and soda pulp bleaching with xylanase (X) and laccase mediator system (LMS) alone and in conjunction (one after the other) (XLMS). METHODS AND RESULTS:Soda and different grades of waste pulp fibres [used for making three-layered duplex sheets - top layer (TL), protective layer (PL) an...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2006.03251.x
更新日期:2007-08-01 00:00:00
abstract:AIMS:The application of essential oils (EOs) and their components as food preservatives is promising but requires a deeper understanding of their mechanisms of action. This study aims to evaluate the effects of thyme EO, carvacrol, citral and 2-(E)-hexenal, on whole-genome gene expression (the transcriptome), as well a...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14048
更新日期:2018-11-01 00:00:00
abstract:AIMS:To evaluate the intraspecific diversity of the fish pathogen Flavobacterium columnare. METHODS AND RESULTS:Genetic variability among Fl. columnare isolates was characterized using restriction fragment length polymorphism analysis of the 16S rDNA gene, intergenic spacer region (ISR) sequencing, and amplified fragm...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2004.02314.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Due to their antimicrobial activity, silver nanoparticles (Ag-NPs) are being increasingly used in a number of industrial products. The accumulation of Ag-NPs in the soil might affect plant growth-promoting rhizobacteria and, in turn, the plants. We describe the effects of Ag-NPs on the soil bacteria Azotobacter vi...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.12779
更新日期:2015-05-01 00:00:00
abstract:AIMS:A culture medium based on apple bagasse was designed and tested as a substrate for biomass production of conventional and unconventional native wine yeasts. METHODS AND RESULTS:The physicochemical characterization of the apple bagasse was carried out and its potential utility as a constituent of a complete cultur...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14216
更新日期:2019-05-01 00:00:00
abstract:AIMS:In order to add to the existing knowledge about structural diversity of biosurfactants, marine environment was chosen to discover a new type of biosurfactant-producing fungus. METHODS AND RESULTS:A number of fungi were collected from the Gulf of Thailand and examined for biosurfactant productivities. A dimorphic ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13598
更新日期:2017-12-01 00:00:00
abstract:AIMS:To investigate the prevalence and genotypic/phenotypic characters of emetic toxin-producing Bacillus cereus strains isolated from sporadic food poisoning cases in Korea. METHODS AND RESULTS:The prevalence of emetic B. cereus was determined in 56 899 stool samples from sporadic food poisoning cases in Korea betwee...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05277.x
更新日期:2012-05-01 00:00:00
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journal_title:Journal of applied microbiology
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2002.01743.x
更新日期:2002-01-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.13386
更新日期:2017-03-01 00:00:00
abstract:AIMS:The absence of standardized methods for quantifying faecal indicator bacteria (FIB) in sand hinders comparison of results across studies. The purpose of the study was to compare methods for extraction of faecal bacteria from sands and recommend a standardized extraction technique. METHODS AND RESULTS:Twenty-two m...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2009.04440.x
更新日期:2009-11-01 00:00:00
abstract::The surface hydrophobicity, viability and biocontrol ability of Penicillium oxalicum spores, produced either in aerial or submerged culture, were characterized. A phase distribution test showed that spores produced in both methods of culture were highly hydrophobic, but those produced in aerial culture were more hydro...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.01189.x
更新日期:2000-11-01 00:00:00
abstract:AIMS:This study aimed to explore the possibility of using the Gluconacin from Gluconacetobacter diazotrophicus strain PAL5 in the biological control of diverse sugarcane phytopathogenic bacteria. METHODS AND RESULTS:An in silico analysis was first employed to determine the phylogenetic relationship between Gram-negati...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14074
更新日期:2018-08-22 00:00:00
abstract:AIMS:To study the succession of cultivated and uncultivated microbes during the traditional curing process of skate. METHODS AND RESULTS:The microbial diversity was evaluated by sequencing 16Sr RNA clone libraries and cultivation in variety of media from skate samples taken periodically during a 9-day curing process. ...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1365-2672.2012.05349.x
更新日期:2012-08-01 00:00:00
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journal_title:Journal of applied microbiology
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更新日期:2018-09-01 00:00:00
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journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1111/jam.14990
更新日期:2021-01-07 00:00:00
abstract::Cattle are considered the major reservoir for Escherichia coli O157:H7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. A sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically...
journal_title:Journal of applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00938.x
更新日期:1999-12-01 00:00:00
abstract::Cancer is a prominent cause of morbidity and mortality worldwide, in spite of advances in therapeutic interventions and supportive care. In 2018 alone, there were 18·1 million new cancer cases and 9·6 million deaths indicating the need for novel anticancer agents. Plant-based products have often been linked with prote...
journal_title:Journal of applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/jam.14981
更新日期:2020-12-23 00:00:00